Role of TGF-β/Smad signaling pathway in pulmonary fibrosis after rheumatoid arthritis in mice北大核心CSCD

Aim To investigate the role of TGF-β/Smad signaling pathway in rheumatoid arthritis (RA) - associated postinterstitial pulmonary fibrosis in mice. Methods The mouse model of RA was constructed by subcutaneous administration of complete Freund's adjuvant (CFA) and chicken II collagen (Col-II) to the tail root of mice. The blank group was given the same amount of distilled water, and the control group was given the same amount of glacial acetic acid (solvent). The degree of toe swelling (joint swelling degree and arthritis index) was monitored to evaluate the mouse modeling. The pathological changes of mouse lung tissues were observed by HE and Masson staining. The expression of TGF-β in lung tissues were observed by immunohistochemical staining. The level of hydroxyproline in lung tissues was measured by chemiluminescence method. The expressions of Smad2, Smad3 and phosphorylated p-Smad2 and phosphorylated p-Smad3 in lung tissues were detected by Western blot. Results Compared with blank group and solvent group, the joint swelling and arthritis index of model group significantly increased. Twenty-one days after administration, HE staining showed inflammatory changes in lung interstitium of the model group, Masson staining showed collagen fiber deposition and obvious fibrosis in lung interstitium of the model group, and immunohistochemical staining showed that the expression of TGF-β in cytoplasm of lung interstitial cells of the model group increased, which was brown and yellow. Meanwhile, hydroxyproline was significantly raised in lung tissue homogenate of the model group. Further WB analysis showed that compared with blank group and solvent group, the expression of p-Smad2 and pSmad3 in lung tissues of the model group was significantly up-regulated (P < 0. 05, P < 0. 01). Conclusions RA can give rise to pulmonary fibrosis, and the expressions of p-Smad2 and p-Smad3 are up-regulated, which is be pivotal in pulmonary fibrosis and RA-related post-interstitial pulmonary fibrosis. © 2023 Publication Centre of Anhui Medical University. All rights reserved.     

Continuous positive airway pressure treatment on blood pressure rhythm in hypertension patients with obstructive sleep apnea

<正>Objective To investigate the effect of continuous positive airway pressure(CPAP) on blood pressure and blood pressure rhythm in hypertensive patients with sleep apnea syndrome.Methods Hypertensive patients with obstructive sleep apnea syndrome consecutively recruited between 2020 and 2022 were randomly divided into two groups,the CPAP group and the control group.Both groups were routinely treated with antihypertensive drugs,and patients in the CPAP group were additionally treated with C...     

多发性甲状腺结节伴癌变456例临床分析

[目的]探讨多发性甲状腺结节伴癌变的诊断、术式选择。[方法]回顾性分析2000年1月至2010年1月治疗的456例多发性甲状腺结节伴癌变患者的临床病理特征、手术方式等临床资料。[结果]456例甲状腺多发结节伴甲状腺癌中,合并结节性甲状腺肿、桥本氏病、甲状腺腺瘤等良性疾病伴发乳头状癌431例,占94.5%,滤泡状癌21例,占4.6%,髓样癌4例,占0.9%。[结论]甲状腺结节数目与甲状腺癌无直接关系,不能将结节数目作为判断甲状腺结节是否为癌的依据。对手术切取的结节应常规行术中冰冻病理切片,明确性质以指导手术方式。