5种黄连生物碱大鼠体外肝代谢特征比较

目的 探讨黄连中5种结构相似异喹啉生物碱大鼠体外肝代谢的选择性。方法 采用大鼠体外肝微粒体温孵方法,通过考察温孵时间、微粒体蛋白浓度以及底物浓度对小檗碱、巴马汀、黄连碱、表小檗碱和药根碱代谢的影响,求得代谢反应动力学参数K_m、V_max和CL_int。结果 5种黄连生物碱成分的体外肝代谢动力学参数K_m和CL_int间差异明显（P＜0.05）。结论 黄连中5种异喹啉生物碱大鼠体外肝代谢存在显著选择性。     

毛细管气相色谱法定量测定壬代环戊双醚

采用毛细管柱定量法测定壬代环戊双醚,结果:线性范围为0.28～15 μg,线性方程Y=1.7015×105 0.086562×107 X,平均回收率为99.98%,满足定量分析的要求.     

双侧海绵窦段巨大动脉瘤分期颅内外血管架桥的护理

颈内动脉海绵窦段动脉瘤约占颅内动脉瘤的3％～6％。双侧海绵窦段巨大动脉瘤较为少见，治疗难度大，并发症及病死率高。我院采用分期自体血管移植颅内外架桥建立颅内外高流量分流后，再行动脉瘤孤立技术成功治愈1例双侧海绵窦段巨大动脉瘤患者，现将护理体会报道如下。     

气管切开患者鼻饲致食物反流的原因分析和护理对策

由于脑部的各种疾患,神经外科患者会有不同程度的意识障碍.此类患者常会出现呼吸道及脑缺氧等并发症,常采用气管切开,使这些患者不能经口进食,需进行鼻饲饮食.     

经鼻内镜下改良鼻咽血管纤维瘤切除术

如何减少经鼻内镜下鼻咽纤维血管瘤切除术的出血量，缩短手术时间，对该手术的推广非常重要，我们引对此点进行了改进。     

颅内囊肿的内镜治疗

目的探讨神经内镜治疗颅内囊肿的方法。方法采用神经内镜治疗17例颅内囊肿,其中蛛网膜囊肿8例,透明隔囊肿5例,侧脑室内囊肿1例,脑脓肿2例,脑实质内猪囊肿虫病囊肿1例。结果本组病例随访6~24个月,所有囊肿腔缩小或消失,脑组织复位,术后临床症状消失,颅内高压缓解。结论神经内镜手术治疗颅内囊肿微创、安全、高效。     

Conversion of mononuclear cells from human umbilical cord blood into hepatocyte-like cells

Objective: To evaluate the differentiation of human umbilical cord blood cells into hepatocyte-like cells. Methods: Mononuclear cells (MNCs) derived from human umbilical cord blood were isolated using Ficoll. The experiment was derived into 3 categories:(1) MNCs co-cultured with 50 mg minced liver tissue separated by a trans-well membrane and then collected at 0 h,24 h,48 h and 72 h; (2) MNCs cultured along supplemented with 100 ml/L FBS. 100μ/ml penicillin, 100μg/ml streptomycin. 4. 7μg/ml linoleic acid, 1×ITS, 10-4 mol/L L-Ascorbic acid 2-P and a combination of FGF4 (100 ng/ml) and HGF (20 ng/mL). Cells were then collected at 0 d and 16 d to examine the expression profile of hepatocyte correlating markers;(3) 0. 2-0. 3 ml of MNCs with a cell density of 2×107/ml were transplanted into prepared recipient mice [n = 12, injected with 0. 4 ml/kg (20%) CCl4 and 150 ng/kg 5-fluorouracil (5-Fu) prior the transplant 24 h and 48 h, respectively] via injection through tail vein. Mice were sacrificed 4 weeks after transplantation. The hepatocyte correlating mRNAs and proteins were determined by RT-PCR, immunohistochemical analysis and immunoflurence technique. Results: (1) After 72 h. a number of glycogen positive stained cells were observed with MNCs co-cultured with damaged mouse liver tissues. The expression of hepatocyte markers, human albumin (ALB),α-fetal protein (AFP) and human GATA4 mRNA and proteins were detected by RT-PCR and immunohistochemistry as well. For the confirmation, the DNA sequencing of PCR products was performed. In control groups, MNCs co-cultured with normal mouse hepatocytes or MNCs cultured alone, all markers remained negative. (2) In growth factor supplemented culture system, MNCs developed into larger volume with richer cytoplasm and binucleation after 16 d. Positive expression of ALB, AFP, CK18 and CK19 mRNA were detected with RT-PCR, and ALB positive staining was observed by immunocytochemistry as well. In contrast. MNCs cultured without exogenous growth factors scarcely attached to the culture dish and ALB mRNA was not detected. (3) In transplantation experiment, both of ALB and AFP mRNA were detected by RT-PCR and HSA, PCNA and ALB positive staining were observed in the livers of recipient mice by immunocytochemistry. Conclusion: MNCs from human umbilical cord blood could convert into hepatocyte-like cells in 3 different ways, indicating their potential use in the clinic applications for the treatment of human liver diseases.     

康欣口服液质量标准的改进

目的建立改进康欣口服液标准的方法。方法采用薄层色谱(TLC)法对康欣口服液中的丹参、地骨皮进行定性鉴别;采用高效液相色谱(HPLC)法测定淫羊藿苷的含量。结果定性鉴别方法专属性强。在5.36~26.8μg范围内淫羊藿苷峰面积与浓度线性关系良好,r=0.999 8;样品平均加样回收率为100.4%(n=9),RSD为1.84%。结论该法简便、快速、结果准确,可有效控制康欣口服液的质量。     

中西医结合治疗肛周尖锐湿疣72例

目的 探讨减少和防止肛周尖锐湿疣电离子治疗术后感染和复发的方法.方法 肛周尖锐湿疣患者共102例,分为治疗组72例,对照组30例.对照组使用CHR-IIB型多功能治疗仪,术后创面用红霉素软膏涂擦;治疗组除对照组方法外,结合术后中药熏蒸坐浴,每日1次,连续7～10 d为1个疗程.结果 治疗组有效率94.4%,感染率1.4%,复发率2.8%;对照组有效率70.0%,感染率33.3%,复发率33.3%.结论 电离子治疗仪治疗肛周尖锐湿疣术后结合中药熏蒸坐浴治疗疗效较好,感染率和复发率低.     

印迹基因H_(19)在少弱精子症中印迹状态的检测

目的:通过研究印迹基因H19在少弱精子症中的印迹状态,探讨H19与精子发生障碍的相关性。方法:采用聚合酶链式反应与逆转录-聚合酶链式反应,结合限制性片段长度多态性分析法(RFLP),对36例异常精液和15例正常精液标本进行H19印迹状态检测。结果:15例正常精液没有发现印迹丢失,36例异常精液检测出3例印迹丢失,印迹丢失率为8.3%。结论:H19印迹丢失与精子发生障碍有关,可能影响精子的数量。