人体肝癌和癌周宿主肝甲种胎儿蛋白免疫酶标记定位及其形态学的观察

近年来,国内外一些学者为了探索甲胎蛋白的细胞来源,及其与肝癌发生发展的关系,采用各种免疫标记技术对甲胎蛋白在动物引癌过程、胚胎肝和人体肝癌细胞中的分布、标记细胞的形态进行了光镜和电镜的观察。本工作使用间接免疫酶标抗体方法,对22例人体原发性肝细胞癌手术切除的标本,进行了有关甲胎蛋白在癌组织,尤其是在癌周宿主肝组织中的分布及其在光学显微镜下的形态学观察。     

SMMC-7721人体肝癌细胞株的电镜和光镜观察

1977年,本室培养建成了SMMC-7721人体肝癌细胞株,迄今已传至120代,可作为肝癌防治和基础理论研究的细胞实验模型。人体肝癌细胞株的建立,国内外均有报道。但专门研究人体肝癌体外培养细胞超微结构的资料尚未见到。因此,在本校电子显微镜室的协助下,我们对此株细胞作了电     

ERYTHROCYTE INSULIN RECEPTORS IN NON-INSULIN DEPENDENT DIABETES MELLITUS

In 49 noninsulin dependent diabetes melrlitus (NIDDM) patients (33 males, 16 females) the con centration decreased of insulin receptor on erythro- cytes and normal levels of fasting plasma insulin as compared with the control groups, suggesting that hyperinsulinemia may not be the sole cause for re ceptor defects in NIDDM. Comparison of insulin receptors between obese and nonobese.NIDDM pa- tients indicated that such defects were more severe in obese NIDDM patients than those in nonobese ones. A slight but signifciant increase of plasma hCH and cortisol was found in NIDDM, but it is unlikely that the decrease of insulin receptors in NIDDM is caused by the sligMy increased antago nistic hormones. The factors leading to insulin re ceptor defects in NIDDM are discussed.     

伯氨喹啉对实验动物心脏的损害——组织学和超微结构的观察

为了观察伯氨喹啉的毒性作用,我们用不同剂量的伯氨喹啉对狗、猴和大鼠作毒性试验,着重检查动物心脏的形态病变。看到大多数中毒狗的心肌有颗粒交性,嗜伊红变性、空泡变性,凝固性坏死和溶解坏死,少数有炎症反应,狗心脏传导系统也有上述多种组织病变。电镜检查见中毒狗心肌纤维内线粒体肿胀、横管肌浆网扩张,肌原纤维密集或溶解、核畸形、染色质凝聚,甚至细胞坏死。病变随用药剂量增大而加剧。这些病变可设想为临床心脏功能变化的形态学证据。实验大鼠也有少数出现心肌嗜伊红变性。实验猴未见明显的心脏病变而有明显的肝脏病变。不同种类的实验动物对伯氨喹啉的中毒反应虽有差异,但心脏损害应视为8-氨基喹啉类药物的重要毒性作用。     

固体激光器恒温控制系统的改进

随着科技的不断发展,越来越多的激光技术被应用在医疗设备中。特别是眼科类激光设备层出不穷,对各类眼科疾病的诊断和治疗带来了极大的发展,为病人解除了病痛。在我国,近十年来借助激光设备开展的治疗近视眼的手术有了很大范围的提升和普及。目前在角膜屈光手术中主要以ArF准分     

多元化合作维修服务模式探索实践

本文对售后服务现状进行分析,提出了多元化合作维修服务模式和采取的具体对策,并应用流程来实现分级管理,从而达到保证维修质量和控制成本.     

Flow Cytometric Analysis of DNA in Bone Tumors Using NucIear Suspension

The types of DNA content ean be divided into four groups:(1)diploids andnera-diploids;(2)triploids;(3)tetraploidy and hyperploidy aneuploids;(4)biclonal DHAcontent.Recent studies show that measuring DNA content by flow cytometry(FCM) can beapplied to most primary bone lumors in pointing out clinicatly relevant informattons.Ii thisstudy,cellular DHA content of 33 primary bone tvenors was analysed by FCM Isolated ratcle-ar suspensions were prepared by a simple,rapid and effective method using 10％ formatin-fctedand paraffin-embeuded bone tumor specimens.The results showed that 10 benign(inchtding 5Grade I giant cell tumors of bone) and 5 histologically questionable tumors had nornufl DNAcontent (diploids or near-diploids)and the other 7 questionable and of the 11 malignanttumors had abnormul DNA content(aneuploids).The cell cycle distribution analysis showedthat the aneuploidy tumors had higher proponion of S-phase and G2+M-phase cells than the nor-mal ploidy tumors,indicating there were differences in proliferative activity.The method alsoshowed that beniga and low-malignant primary bone tumors were diploids or near-diptoids,andhigh-malignant cnes were aneuploids Compared with typically pathological grading,the flowDNA analysis of bone tumors can more objectively point out their biological behavior andprognosis.     

黄曲霉毒素B_1诱发大鼠肝癌的癌前病变与肝癌的组织发生学

用大鼠31只,喂食纯品AFB_1诱发肝癌观察其癌前病变及肝癌的组织发生学。其癌前病变有肝细胞不典型增生(58.06％);嗜酸性细胞增生灶(51.61％),用IIP-AFP多呈阳性,嗜碱性细胞增生灶(29.03％),IIP-AFP呈阴性。诱发出肝癌11例(35.48％),多中心起源7例、单中心起源4例。Ⅰ级分化9例,Ⅱ级分化2例。大细胞性肝癌7例,作IIP-AFP3例,有2例阳性,小细胞性肝癌4例,2例作IIP-AFP,1例阳性。6例肝癌作电镜观察显示大细胞性肝癌分化较好、小细胞性肝癌分化较差的亚微结构。其核仁均质化较为特殊。作者提出了AFB_1诱发肝癌的组织发生的设想,并认为肝癌的多中心与单中心起源均可存在。     

新抗疟化合物的临床前毒性研究——Ⅴ.5-对氟苯氧基伯喹柠檬酸盐的急性、亚急性毒性观察(1)

磷酸伯氨喹啉(简称伯喹)是间日疟治疗中迄今为止,视为毒性最小、效果最好的抗复发药。即使这样,伯喹仍有疗程过长,毒性较大等缺点,临床应用受到一定的限制。为此,多少年来国内外学者致力于伯喹结构的改造和构效关系的探索,借以寻找高效低毒的新抗复发药,已成为当前抗疟药研究中     

当前医疗设备维修服务环境SWOT分析与战略思考

本文采用SWOT分析法,对医疗设备维修服务的内外因素进行分析,找出作为医院的内在优势和弱势,外在机会和挑战,并提出在医疗设备维修服务战略方面的进攻、调整、防御和生存的四种策略的思考.