Synthesis and biocompatibility of an experimental glass ionomer cement prepared by a non-hydrolytic sol-gel method

The aims of this study were to demonstrate the synthesis of an experimental glass ionomer cement (GIC) by the non-hydrolytic sol-gel method and to evaluate its biocompatibility in comparison to a conventional glass ionomer cement (Vidrion R). Four polyethylene tubes containing the tested cements were implanted in the dorsal region of 15 rats, as follows: GI - experimental GIC and GII - conventional GIC. The external tube walls was considered the control group (CG). The rats were sacrificed 7, 21 and 42 days after implant placement for histopathological analysis. A four-point (I-IV) scoring system was used to graduate the inflammatory reaction. Regarding the experimental GIC sintherization, thermogravimetric and x-ray diffraction analysis demonstrated vitreous material formation at 110oC by the sol-gel method. For biocompatibility test, results showed a moderate chronic inflammatory reaction for GI (III), severe for GII (IV) and mild for CG (II) at 7 days. After 21 days, GI presented a mild reaction (II); GII, moderate (III) and CG, mild (II). At 42 days, GI showed a mild/absent inflammatory reaction (II to I), similar to GII (II to I). CG presented absence of chronic inflammatory reaction (I). It was concluded that the experimental GIC presented mild/absent tissue reaction after 42 days, being biocompatible when tested in the connective tissue of rats.     

Preventive effect of an iron varnish on bovine enamel erosion in vitro

Objective

The aim of this study was to evaluate, in vitro, the effect of an experimental varnish containing iron on the dissolution of bovine enamel by carbonated beverage.

Methods

Eighty specimens were randomly allocated to four groups (n = 20 per group), according to the following treatments: Fe varnish (FeV, 10 mmol/L Fe), F varnish (FV, 2.71% F), placebo varnish (PV) and control (not treated, NT). The varnishes were applied in a thin layer and removed after 6 h. Then, the samples were submitted to six cycles, alternating re- and demineralisation (only 1 day). Demineralisation was performed with the beverage Coca-Cola^® (10 min, 30 mL/block) and remineralisation with artificial saliva for 1 h. In order to determine the amount of enamel dissolved, the wear was analysed by profilometry. Data were analysed by ANOVA and Tukey's test (p < 0.05).

Results

The mean wear (±S.E.) was significantly lesser for the FeV (0.451 ± 0.018 μm) when compared to the other treatments. The FV caused significantly less wear (0.554 ± 0.022 μm) when compared to PV (0.991 ± 0.039 μm) and NT (1.014 ± 0.033), which did not significantly differ from each other.

Conclusions

The results suggest that the iron varnish can interfere with the dissolution of dental enamel in the presence of acidic beverages.     

Proliferative activity in oral salivary gland tumors: the role of PCNA and AgNOR assessed by a double staining technique

The aim of this study was to evaluate the role of PCNA and AgNOR in the assessment of salivary gland tumor proliferation using a double staining technique. Ten cases of pleomorphic adenoma (PA) and seventeen cases of adenoid cystic carcinoma (ACC) were examined. Numeric and morphometric parameters of AgNOR were evaluated and compared in PCNA-positive and PCNA-negative nuclei. There were statistically significant differences in AgNOR numbers, perimeters and contour indices between PCNA-positive and -negative nuclei in the PA samples. The ACC samples demonstrated significant differences only in the AgNOR areas. Our results show that in salivary gland tumors there is not always a relationship between proliferative activity evaluated by AgNOR numeric and morphometric parameters and PCNA immunostaining.     

Delayed formation of a lower second premolar

Delayed odontogenesis of a lower second premolar is presented in a case treated without extraction and focuses on the 7-year follow-up of the delayed tooth bud. The follow-up was initiated when the crown formation was diagnosed and was finished when the tooth erupted completely into the orthodontically provided space, which enabled the orthodontic leveling and alignment of the delayed premolar. The long follow-up indicates that delayed tooth buds may develop completely and normally.     

Evaluation of oral and nasal odor in patients with and without cleft lip and palate: preliminary report.

OBJECTIVE: To evaluate oral and nasal halitosis parameters in patients with and without clefts. DESIGN: Randomized and prospective study. Patients with and without clefts were evaluated as to oral and nasal halitosis. SETTING: University of S?o Paulo, Bauru Dental School and Hospital for Rehabilitation of Craniofacial Anomalies, Bauru, Brazil. PATIENTS: Twelve patients with clefts and 12 without clefts were evaluated, and no exclusion criteria were followed. INTERVENTIONS: Nasal and oral halitosis were measured with the use of a portable sulfide monitor during a single visit. RESULTS: One hundred percent of the patients with clefts had altered values for volatile sulfur compounds. Only 33.3% of the patients without clefts had at least one strong value for nasal halimeter measurements, and 58.3% of these patients showed alteration in the nasal values. Statistical evaluation, made using the Mann-Whitney U test, classifying the nasal halimeter values as normal, weak, and strong, showing a statistical significant group difference (p = .003). There was no significant difference in the oral halimeter values between the two groups. CONCLUSIONS: Patients with cleft lip and palate had a tendency to present higher values for the nasal halimeter measurements, when compared with patients without clefts.     

Bifid mandibular condyle: case report and etiological considerations

Bifid mandibular condyle, usually diagnosed on routine radiographic examination, is described in the literature as a rare entity. Its cause is controversial, and it has no predilection by sex or ethnic background. Dental professionals should have some knowledge of this anatomic abnormality, as well as its implications for function and appropriate treatment modalities, so that they can be alert to this potential diagnosis. This paper reports an unusual case of bifid mandibular condyle with possible traumatic cause, with emphasis on the radiographic and tomographic findings.     

Eruption of teeth associated with a dentigerous cyst by only marsupialization treatment: a case report

The purpose of this case report was to describe the management of a mandibular dentigerous cyst in a 10-year-old male patient. Without any clinical symptom, the lesion was detected in a routine panoramic radiograph showing a well-defined osteolytic lesion that measured 3 cm in diameter, partially surrounding the crown of the canine, first molar, and second premolar displacing the teeth to the mandible's lower border. The apex of the teeth were still open. Under local anesthesia, the patient was treated by: extraction of the left deciduous canine and first and second deciduous molars; and marsupialization of the cystic cavity. After 30 months, the premolar had erupted and an orthodontic therapy was started to maintain space for the canine.