A VAM-Based Equivalent Model for Triangular Honeycomb Sandwich Panels: Comparison with Numerical and Experimental Data

Due to their complex microstructures, the research on the static and dynamic behaviors of triangular honeycomb sandwich panels (triangular HSPs) is limited. In this study, the effective plate properties of triangular HSP was obtained by the homogenizing of the unit cell, and then the input to a VAM-based two-dimensional equivalent plate model (2D-EPM) to perform static and dynamic analyses. The accuracy of the proposed model for predicting the equivalent stiffness of the triangular HSP was verified by three-point bending experiments of 3D-printed specimens. Then, the static displacement, global buckling, and free vibrations predicted by 2D-EPM were verified with the results from three-dimensional finite element model simulations under various boundary conditions. The influences of structural parameters (including angle, core wall thickness, and cell side length of the unit cell) on the static and dynamic characteristics of triangular HSPs were also investigated, which can provide a useful tool for the modeling and evaluation of triangular HSPs under different conditions.     

Antigenicity Alternations of Variant PEDV S Protein Disclosed by Linear B Cell Epitope Mapping

The spike protein (S) plays a crucial role in porcine epidemic diarrhea virus (PEDV) infection and induces neutralizing antibodies. Mutations of the S protein are supposed to provide the main antigenic shift leading to the antigenic escape of PEDVs. It is therefore a significant question how much accumulation of antigenic shift could lead to the antigenic escape of the variant PEDV. To provide an answer in the study, B cell epitopes (BCEs) on the S protein of the PEDV vaccine strain CV777 (S^CV777) and variant strain SD2014 (S^SD2014) were mapped using biosynthetic peptides and rabbit anti-PEDV S serum. Seventy-nine and 68 linear BCEs were identified from S^CV777 and S^SD2014, respectively. While 66.2% of the BCEs of S^SD2014 could be recognized by anti-S^CV777 serum and 67.1% of S^CV777 BCEs could be recognized by anti-S^SD2014 serum, more than 40% of the BCEs identified using anti-S^CV777 serum on S^CV777 could not be recognized by anti-S^SD2014 serum and vice versa. The completely shared BCEs took low percentages of 29.4% and 25.3% for S^SD2014 and S^CV777, respectively. These results indicate a low conservation of antigenicity of the S protein compared to a relatively high amino acid sequence similarity of 92.2% between the two strains. The study provided a BCE shift reference of PEDV antigenic escape and surveillance control.     

许旺细胞的IGF-Ⅰ基因转染及其mRNA和蛋白表达

为探索类胰岛素生长因子Ⅰ（ＩＧＦⅠ）基因转染许旺细胞的可能性,采用体外培养许旺细胞（ＳＣｓ）,构建ＩＧＦⅠ逆转录病毒载体,利用基因转染技术将ＩＧＦⅠ基因转入ＳＣｓ,核酸分子杂交及细胞免疫组化检测ＳＣｓ 表达ＩＧＦⅠｍ ＲＮＡ及其蛋白水平,辅以正常ＳＣｓ作对照。结果：ｐＬＸＳＮＩＧＦⅠ转染的ＳＣｓ在体外相同培养条件下较正常ＳＣｓ 表达ＩＧＦⅠｍ ＲＮＡ 及其蛋白水平能力分别增强４８ 和３２ 倍,均有显著意义。研究表明,利用基因转染技术可将外源性ＩＧＦⅠ基因转入ＳＣｓ,提高ＳＣｓ中的ＩＧＦⅠ的含量和活性     

蝶枕角与颅底诸结构至耳眼平面垂直距离的纵向比较

目的:为人颅底的生长发育研究提供正常的颅底结构测量数据和参照标准;初步分析颅底生长发育趋势和特点。方法:对人不同生长发育阶段颅底标本诸结构进行观察和测量,并对其进行相互比较和纵向分析。结果:颅底在不同发育阶段各有其趋势和特点,蝶枕角随着年龄的增长逐渐变小,但性别之间无差异,颅前窝诸结构变化幅度最大,颅中窝的变化相对最小,11项测量指标在不同生长发育阶段的变化各异。结论:观察测量数据可为人颅底生长发育进一步研究提供依据,蝶枕角、耳眼(FH)平面可作为颅底生长发育的观察测量指标和参照平面。     

30 d-6°头低位卧床对人下肢脂肪体积的影响

目的 观察模拟失重对人下肢脂肪体积的影响,验证磁共振水脂分离成像技术在测量人下肢脂肪容积中的可行性.方法 14名男性健康志愿者,-6°头低位卧床30 d,使用磁共振水脂分离成像(Dixon)技术采集卧床前后的下肢横断面解剖图像,测量卧床前后下肢脂肪体积.结果 与卧床前相比,卧床后所有志愿者下肢总脂肪体积及大腿段脂肪体积显著增加(P＜0.001);小腿段脂肪体积呈增加趋势,但无统计学差异(P＞0.05).结论 使用MRI水脂分离成像可以清晰地显示卧床前后下肢脂肪体积的变化;-6°头低位卧床30 d后下肢脂肪体积明显增加.     

小儿乙状结肠冗长症21例诊治分析

目的总结小儿乙状结肠冗长症的临床特点、诊断及治疗方法。方法回顾性分析我院近7年来收治的21例乙状结肠冗长症患儿的病例资料,并结合文献讨论临床表现及诊疗方法。结果21例均获痊愈,经3个月～6年随访,患儿排便功能恢复,发育状况良好。结论小儿乙状结肠冗长症以慢性便秘和间歇性腹痛为主要症状;诊断主要根据钡剂灌肠检查,部分病例应与先天性巨结肠、特发性巨结肠鉴别;治疗方法首选非手术疗法,无效时行手术治疗。     

The remedial effect of soluble interleukin-1 receptor type Ⅱ on endometriosis in the nude mouse model

Objective： Recent studies have shown that the local expression of soluble interleukin （IL） -1 receptor type Ⅱ （slL-1 R Ⅱ ） in endometrial tissue of women with endometriosis is decreased, and the depression of IL-1 R Ⅱ was more significant in infertile women than that in fertile women with endometriosis. In this research, we investigated the remedial effect of slL-1-R Ⅱ administration on endometriosis in the nude mouse model. Methods： Nineteen nude model mice with endometriosis were randomly divided into three groups： group A was treated by intraperitoneal administration with only slL-1 R Ⅱ for two weeks, group B was similarly treated with only IL- 1, and group C （control） was administered saline. After 2 weeks, the size of the ectopic endometrial lesions was calculated, and the expression of vascular endothelial growth factor （VEGF） and B-cell lymphoma leukemia-2 （Bcl- 2） were detected by immunohistochemistry. The IL-8 and VEGF levels in the peritoneal fluid （PF） and serum were also measured by enzyme-linked immunosorbent assay （ELISA）. Results： The mean size of ectopic endometrial lesion did not differ between the three groups （P 〉 0.05）. Compared with the control, the expression of VEGF and Bcl-2 was significantly lower in group A, and higher in group B. In the three groups, the levels of IL-8 in the PF and serum were highest in group A, and lowest in group B. Conclusion： slL-1 R Ⅱ may suppresse hyperplasia of ectopic endometriosis, perhaps by reducing the expression of certain cytokines, such as VEGF, IL-8, and Bcl-2, which could provide a new clinical strategy for the treatment of endometriosis.     

踝关节骨折治疗体会

目的:通过分析骨折受伤机制,探讨骨折治疗方法。方法:采取保守、手术及中药辨证施治。结果:平均随访时间8个月,优良率达95%。结论:明确受伤机制,准确的诊断分型及辨证施治可以取得良好的疗效。     

An antibody against Siglec-15 promotes bone formation and fracture healing by increasing TRAP+mononuclear cells and PDGF-BB secretion

INTRODUCTION Siglec-15 is a member of the sialic acid-binding immunoglobulintype lectin (Siglecs) family, a group of cell-surface receptors that potentially reg...     

多药耐药基因相关蛋白在原发性肝癌中的表达及意义

目的探讨原发性肝细胞癌中耐药基因相关蛋白P-糖蛋白(P-gp)、DNA拓扑异构酶-Ⅱ(Topo-Ⅱ)、谷胱苷肽-S-转移酶(GST-π)的表达及其意义。方法应用免疫组织化学方法S-P法检测49例原发性肝癌组织、14例肝硬化组织及13例正常肝组织中P-gp、Topo-II、GST-π的表达。结果肝癌组三者表达均高于肝硬化组及正常组(P<0.05),肝硬化组与正常组之间两者表达差异无统计学意义。P-gp表达与肿瘤Edmondson分级呈负相关;Topo-II表达则与Edmondson分级和转移呈正相关。P-gp、Topo-II表达呈负相关。结论联合检测肝癌耐药基因相关蛋白P-gp、Topo-II、GST-π的表达有助于临床判断肝癌对化疗的敏感性及预后。