隐动脉筋膜蒂的交腿皮瓣修复小腿严重软组织缺损

目的探讨一种小腿严重软组织缺损的修复方法。方法以健侧隐动脉为蒂的顺行或逆行交腿皮瓣修复患侧小腿软组织缺损。结果本组8例均有效地覆盖受区创面,随访三个月,皮瓣全部成活。结论该皮瓣不牺牲小腿的重要血管,质地与受区相似,方法简单易行,血运可靠,血管蒂长,是修复小腿严重软组织缺损的较好方法。     

果胶酶治疗胃石症的临床研究

目的 探讨胃石症的发病机制。分析和总结果胶酶治疗胃石症的疗效。方法 在体外将山楂，柿子嚼碎后置入新鲜胃液，分为加白酒和不加白酒两组后放入37℃恒温箱内，观察胃石形成的经过及时间，再分别加入果胶酶，观察其解聚过程及时间，对54例胃石症患服用果胶酶治疗并观察疗效。结果 加白酒组形成胃石的速度较快，果胶酶能有效溶解胃石。54例患服用果胶酶6h后吞钡透视见胃石完全消失，症状，体征完全缓解。结论 果胶酶能有效溶解胃石。是治疗胃石症的有效方法。     

舰船员慢性荨麻疹与马拉色菌相关性研究

OBJECTIVE: To investigate the association of Malassezia furfur with chronic urticaria in the crew members of ships. METHODS: A comparative mycological study of 126 crew members of ships with chronic urticaria and 45 normal control subjects was carried out. The 82 urticaria patients identified as positive for Malassezia furfur were divided into groups A and B to receive treatment with antihistaminics (group A) and antihistaminics combined with 2% ketoconazole shampoo(group A). RESULTS: The carrier rates of Malassezia furfur were significantly higher in urticaria patients than in the normal control subjects (P<0.01), but in view of the case ratios of the final cure or improvement, no significant difference was observed between the two groups by the end of the treatment courses (P>0.05). But 6 to 8 weeks from the end of the treatment course, better therapeutic effect was noted in group B (P<0.01), with higher rate of negative Malassezia furfur findings (P<0.01). CONCLUSION: Malassezia furfur may play an important role in the prevalence of chronic urticaria among the crew members, and anti-fungal treatment may produce better long-term therapeutic effect.     

重症SARS的X线影像表现

目的探讨重症严重急性呼吸综合征(SARS)的X线表现.方法回顾性分析37例重症SARS患者的一系列X线胸片变化规律.结果重症SARS患者胸片表现为两肺阴影迅速进展,大部分区域受累.有3种表现类型:①19例表现为弥漫大片云雾状影,病灶无明显分界,肺纹理显示不清;②13例表现为弥漫棉团状密影,边缘模糊,肺纹理增粗;③5例表现为多发大团块密影,边缘模糊,可见支气管气相.如无合并症发生,对症治疗有效,1个月左右肺部阴影消失.6例肺部合并G-杆菌感染,5例合并纵隔气肿,出现合并症时,肺部阴影延迟吸收.结论重症SARS患者的X线影像为肺部密影快速进展至两肺大部分区域,易合并纵隔气肿及肺部感染.     

Effect of Sirpα1 on the expression of nuclear factor-kappa B in hepatocellular carcinoma

BACKGROUND:Signal regulatory protein alpha1(Sirpα1) is a member of Sirps families containing four immunoreceptor tyrosine-based inhibitory motifs(ITIMs) domains in the cytoplasm of and an activated substrate of receptor tyrosine kinase(RTK),that negatively regulates the RTK-dependent cell proliferating signal transduction pathway.Previously we found that Sirpα1 was closely associated with the occurrence and development of hepatocellular carcinoma(HCC)as well as liver regeneration.Since it is unclear about the regulatory mechanisms,we established the cell line transfected Sirpα1 gene and preliminarily clarified the mechanisms by which Sirpα1 negatively regulates the carcinogenesis and development of HCC. METHODS:Liver cancer Sk-Hep1 cell was respectively transfected with plasmids of pLXSN,pLXSN-Sirpα1 and pLXSN-Sirpα1Δ4Y 2 ,screened with the drug of G418(1200 μg/ml),and various transfected Sk-Hep1 cell lines were obtained.The protein expressions of P65,P50,IκBα,cyclin D1 and Fas in various Sk-Hep1 cell lines were determined by Western blotting,and P65 and P50 were localized by the immunofluorescence technique. RESULTS:Sirpα1 could significantly upregulate the protein expression of IκBα(vs.other cell lines,P<0.05) in the Sk-Hep1 cell,and downregulate the protein expressions of P65,P50 and cyclin D1(vs.other cell lines, P<0.05)in the Sk-Hep1 cell.P65 protein expression was mainly localized in the cytoplasm in the pLXSN Sk-Hep1 cell,and in the nucleus of the Sk-Hep1 cell with mutantSirpα1Δ4Y 2 ,but in nucleus of the Sk-Hep1 cell with wild Sirpα1.P50 protein expression was localized in the cytoplasm and nucleus of the pLXSN Sk-Hep1 cell,but in the nucleus of the Sk-Hep1 cell with wild Sirpα1 and mutant Sirpα1Δ4Y 2 plasmid. CONCLUSIONS:Sirpα1 might negatively regulate and control the abnormal proliferation of liver cancer cells by influencing the protein content and localization of nuclear factor-kappa B,then influence the expression of cyclins such as cyclin D1 in the signal transduction pathway.It may be one of the important mechanisms by which Sirpα1 negatively regulates the carcinogenesis and development of HCC.     

微波治疗慢性单纯性鼻炎 205 例

目的　探讨微波治疗慢性单纯性鼻炎的疗效。方法　采用EBH IV型耳鼻喉综合微波治疗仪治疗慢性单纯性鼻炎 2 0 5例 ,之后用达芬霖液体喷雾剂喷鼻 1～ 2周 ,1个月后检查统计分析治疗结果。结果　治愈 181例 (88.3% ) ,好转 2 2例 (10 .7% ) ,无效 2例 (1.0 % ) ,总有效 2 0 3例 (99.0 % )。结论　应用微波治疗慢性单纯性鼻炎具有操作简单、无出血及焦痂和治疗效果好等优点     

新型重组人肿瘤坏死因子药代动力学的实验方法

目的 为探讨新型重组人肿瘤坏死因子（nrTNF α）药代动力学而建立生物样品中nrhTNFα的检测方法。方法 （1）方法Ⅰ（RA）：动物肌注^125 Ⅰ标记的nrhTNFα后，测定各生物样品中的总放射活性；（2）方法Ⅱ（HPLC－RA）；上述生物样品无经HPLC分离后，收集并测定其中^125Ⅰ－nrhTNFα部分的放射活性；（3）方法 ⅢELISA法。结果 RA，HPLC－RA法检测的nrhTNFα质量浓度与放射性呈直线相关（RA，r=0.999HPLC－RA,r=0.999)。ELISA法证明nrhTNFα的酶联免疫检测药盒适用于nrhTNFα的检测，nrhTNFα的标准曲线与nrhTNFα的标准曲线显正相关，r=0.994,这一检测系统与其他细胞因子包括IFNα，TNFβ，IL－2和IL－6无交叉反应。结论 RA，HPLC－RA和ELISA法均可作为nrhTNFα的药代动力学实验方法。     

早期培养学生临床思维能力的探讨

临床思维能力是临床能力的核心和基础,提高临床思维能力是医学教育的终极目标之一,学生应尽早得到临床思维能力的培养和训练。通过对常用诊疗技术课程教学进行相应的改革实践,即应用多媒体技术支持的病案教学与临床见习整合的方法,培养学生的临床思维能力,经实践观察,这些教改措施与提高学生的临床思维能力有正相关关系。     

肝内HBcAg与血清学指标的关系及慢性乙型肝炎发病机理的探讨

We detected the presence and distribution of HBcAg in the liver by immunohistochemistry (ABC method) and the presence of HBV-DNA in serum (spot hybridization) and anti-HBe in serum (ELISA) from 59 cases of hepatitis B hospitalized in our hospital, including 47 cases of CAH, 5 cases of CPH, and 7 cases of subacute fulminant hepatitis. 1. HBcAg in the liver was detected in 25 out of 47 cases (53%) of CAH, in 2 out of 5 cases of CPH and in 4 out of 7 cases of subacute fulminant hepatitis. The total percentage was 53% (31/59). 2. There was no positive correlation between HBV replication activity and liver disease activity (P greater than 0.05). Our results did not support the hypothesis that suggests a direct cytopathic effect of HBV. Oppositely, the fact was that the presence, the amount and the patterns of HBcAg in the liver, and the presence of HBV-DNA in serum were predominant in mild CAH compared with those in severe CAH, predominant in CAH without cirrhosis compared with those in CAH with cirrhosis. There was a tendency of inverse correlation between HBV replication activity and liver disease activity. The results above were in line with the concept that HBcAg expressed on the surface of infected hepatocytes may be relevant target for T lymphocyte cytotoxicity. The results have suggested that an immune response to HBV is present, leading to the destruction of most infected cells. 3. There was a positive correlation between HBV-DNA in serum and HBcAg in the liver (P less than 0.005), indicating that HBV-DNA in serum can represent HBV replication.(ABSTRACT TRUNCATED AT 250 WORDS)