Neurogenesis in the adult hippocampus: history,regulation, and prospective roles

Background: The hippocampus is one of the sites in the mammalian brain that is capable of continuously generating controversy. Adult neurogenesis is a remarkable process, and yet an intensely debatable topic in contemporary neuroscience due to its distinctiveness and conceivable impact on neural activity. The belief that neurogenesis continues through adulthood has provoked remarkable efforts to describe how newborn neurons differentiate and incorporate into the adult brain. It has also encouraged studies that investigate the consequences of inadequate neurogenesis in neuropsychiatric and neurodegenerative diseases and explore the potential role of neural progenitor cells in brain repair. The adult nervous system is not static; it is subjected to morphological and physiological alterations at various levels. This plastic mechanism guarantees that the behavioral regulation of the adult nervous system is adaptable in response to varying environmental stimuli. Three regions of the adult brain, the olfactory bulb, the hypothalamus, and the hippocampal dentate gyrus, contain new-born neurons that exhibit an essential role in the natural functional circuitry of the adult brain. Purpose/Aim: This article explores current advancements in adult hippocampal neurogenesis by presenting its history and evolution and studying its association with neural plasticity. The article also discusses the prospective roles of adult hippocampal neurogenesis and describes the intracellular, extracellular, pathological, and environmental factors involved in its regulation. Abbreviations AHN Adult hippocampal neurogenesis

AKT Protein kinase B

BMP Bone Morphogenic Protein

BrdU Bromodeoxyuridine

CNS Central nervous system

DG Dentate gyrus

DISC1 Disrupted-in-schizophrenia 1

FGF-2 Fibroblast Growth Factor 2

GABA Gamma-aminobutyric acid

Mbd1 Methyl-CpG-binding domain protein 1

Mecp2 Methyl-CpG-binding protein 2

mTOR Mammalian target of rapamycin

NSCs Neural stem cells

OB Olfactory bulb; P21: cyclin-dependent kinase inhibitor 1

RBPj Recombination Signal Binding protein for Immunoglobulin Kappa J Region

RMS Rostral migratory Stream

SGZ Subgranular zone

Shh Sonic hedgehog

SOX2 SRY (sex determining region Y)-box 2

SVZ Subventricular zone

Wnt3 Wingless-type mouse mammary tumor virus

     

Walk-in Model for Ill Care in an Urban Academic Pediatric Clinic

Objectives

Since the Institute of Medicine's 2001 charge to reform health care, there has been a focus on the role of the medical home. Access to care in the proper setting and at the proper time is central to health care reform. We aimed to increase the volume of patients receiving care for acute illnesses within the medical home rather than the emergency department or urgent care center from 41% to 60%.

Rationale and design of the coronary artery calcium consortium: A multicenter cohort study

Background

Although coronary artery calcium (CAC) has been investigated for over two decades, there is very limited data on the association of CAC with cause of death. The CAC Consortium is a large ongoing multi-center observational cohort of individuals who underwent non-contrast cardiac-gated CAC testing and systematic, prospective, long-term follow-up for mortality with ascertainment of cause of death.

Attitudes of Lebanese University Students Towards Surgical Hymen Reconstruction

Many cultural and religious beliefs place virginity at a high level of social significance, in that women who lose their virginity before marriage may face humiliation, ostracism, divorce, and extreme violence. This led to an increase in the demand for virginity restoration through surgical hymen reconstruction among these cultures. However, data regarding the acceptance of hymenoplasty in societies that consider sexuality a taboo are scarce. In this cross-sectional study, we investigated the effects of gender and religion on sexual attitudes towards hymenoplasty, premarital sex, and virginity in a sample of 600 Lebanese university students. Our findings showed that approval of hymenoplasty was low among participants regardless of gender (25.7 % men vs. 19.1 % women) and religious affiliations (22.5 % Muslims vs. 22.3 % Christians). Arguments for rejection were rooted in moral ethics and personal convictions: “form of deceiving and cheating” (80.7 %) and “betrayal of honesty in the relationship” (80.4 %). Reasons for acceptance included: personal belief in “women’s rights, autonomy, and freedom” (72.2 %) and “physical harm and death” (63.5 %).Male participants were more likely to approve premarital coital sex than females (61.0 vs. 27.3 %). Muslims were also more likely to reject marrying a non-virgin than Christians (39.9 vs. 18.0 %). Female participants expressed more tolerance towards marrying a non-virgin male partner (78.3 vs. 57.3 %). Low acceptance of hymenoplasty among Lebanese university students was found to be related to moral ethics and personal convictions independently from gender and religious affiliation. Differences in sexual attitudes towards premarital coital sex and virginity, however, were more significantly influenced by culture and religion.     

Assessment of phytochemical content in human milk during different stages of lactation

ObjectiveThe present study reports the presence of several carotenoids and flavonoids in human milk samples.MethodsSamples were collected from 17 women who delivered healthy term babies (≥37 wk of gestation) at 1-, 4-, and 13-wk postpartum intervals.ResultsEpicatechin (63.7–828.5 nmol/L), epicatechin gallate (55.7–645.6 nmol/L), epigallocatechin gallate (215.1–2364.7 nmol/L), naringenin (64.1–722.0 nmol/L), kaempferol (7.8–71.4 nmol/L), hesperetin (74.8–1603.1 nmol/L), and quercetin (32.5–108.6 nmol/L) were present in human milk samples with high inter-/intraindividual variability. With the exception of kaempferol, the mean flavonoid content in human milk was not statistically different among lactation stages. In contrast, carotenoids α-carotene (59.0–23.2 nmol/L), β-carotene (164.3–88.0 nmol/L), α-cryptoxanthin (30.6–13.5 nmol/L), β-cryptoxanthin (57.4–24.8 nmol/L), zeaxanthin (46.3–21.4 nmol/L), lutein (121.2–56.4 nmol/L), and lycopene (119.9–49.5 nmol/L) significantly decreased from weeks 1 to 13 of lactation.ConclusionThe observed differences in the relative concentrations of the two phytochemical classes in human milk may be a result of several factors, including dietary exposure, stability in the milk matrix, efficiency of absorption/metabolism, and transfer from plasma to human milk. These data support the notion that flavonoids, as with carotenoids, are dietary phytochemicals present in human milk and potentially available to breast-fed infants.     

An evaluation of impression techniques for multiple internal connection implant prostheses

STATEMENT OF PROBLEM: Movement of impression copings inside the impression material using an open-tray impression technique during clinical and laboratory phases may cause inaccuracy in transferring the 3-dimensional spatial orientation of implants intraorally to the definitive cast. Consequently the restoration may require corrective procedures. PURPOSE: This in vitro study evaluated the accuracy of 3 different impression techniques using polyether impression material to obtain a precise definitive cast for a multi-unit implant restoration with multiple internal connection implants. MATERIAL AND METHODS: A reference acrylic resin model with 4 internal connection implants (3i Implant Innovations) was fabricated. Forty-five medium-consistency polyether impressions (Impregum Penta) of this model were made with square impression copings using an open-tray technique. Three groups of 15 specimens each were made with different impression techniques: in the first group, nonmodified square impression copings were used (NM group); in the second group, square impression copings were used and joined together with autopolymerizing acrylic resin before the impression procedure (R [resin] group); and in the third group, square impression copings previously airborne-particle abraded and coated with the manufacturer-recommended impression adhesive were used (M [modified] group). Matching implant replicas were screwed into the square impression copings in the impressions. Impressions were poured with ADA type IV stone (New Fujirock). A single calibrated examiner blinded to the nature of the impression technique used examined all definitive casts to evaluate the positional accuracy (mum) of the implant replica heads using a profile projector (at original magnification x10). These measurements were compared to the measurements calculated on the reference resin model which served as control. Data were analyzed with a 1-way analysis of variance at alpha=.05, followed by the Student Newman-Keuls test (alpha=.05). RESULTS: The data obtained with the profile projector revealed significant differences within the 3 impression techniques ( P <.001). The Student Newman-Keuls procedure disclosed significant differences between the groups, with group R casts being significantly more accurate than group NM and group M casts ( P =.05). The mean distance (+/-SD) between the posterior implants compared to the reference acrylic resin model was 18.17 mum (+/- 6.4) greater for group R casts, 41.27 mum (+/- 8.4) greater for group M casts, and 46.21 mum (+/- 8.9) greater for group NM casts. Distances between the anterior implants were also greater than those recorded on the reference model. The distance was 15.23 mum (+/- 5.9) greater on group R casts, 38.17 mum (+/- 8.3) greater on group M casts, and 43.23 mum (+/- 8.7) greater on group NM casts. CONCLUSION: Within the limitations of this study, improved accuracy of the definitive cast was achieved when the square impression copings joined together with autopolymerizing acrylic resin were used to make an impression of multiple internal connection implants.     

Two patterns of histologic healing in an intrabony defect following treatment with enamel matrix derivative: a human case report

Human histologic evidence of periodontal regeneration following treatment of intrabony defects with enamel matrix derivative has yielded inconsistent results in recent case reports. A 46-year-old woman presenting one deep intrabony defect at the distal root of a mandibular first molar scheduled for extraction was selected for enamel matrix derivative therapy. During surgery, a notch was placed at the most apical level of calculus on the experimental root. Nine months postsurgery, a block section including the distal root and surrounding periodontal tissues was obtained and processed in a mesiodistal plane. Histologic analysis demonstrated two different patterns of healing along the proximal and furcal surfaces. Regeneration with new cellular cementum, bone, and periodontal ligament with functional fiber orientation was observed on the distal aspect of the root, whereas the furcal surface healed through ankylosis. This report underlines the biologic variability in wound healing following enamel matrix derivative therapy in periodontal intrabony defects and within the same defect. Host-specific intrinsic and/or extrinsic factors accounting for this variability remain to be investigated.