一种海洋来源ETP类化合物GQQ-ZML-2抑制H358细胞增殖作用与机制的研究

摘要：目的 探究化合物GQQ-ZML-2对H358细胞(KRASG12C)的增殖抑制作用并初步研究其作用机制。方法 采用磺酰罗丹明B法（SRB法）测定GQQ-ZML-2对不同KRAS突变的肿瘤细胞的细胞毒;采用流式细胞术检测细胞周期和细胞凋亡;利用荧光探针DCFH-DA检测细胞内总活性氧（ROS）水平;Western Blotting检测相关信号蛋白的表达水平;结果 GQQ-ZML-2选择性抑制H358细胞增殖,以浓度依赖的方式抑制克隆形成并将细胞周期阻滞于G2/M期而诱导细胞凋亡。机制研究表明GQQ-ZML-2引起KRAS G12C依赖的ROS产生,抑制AKT/mTOR和JAK2/STAT3信号通路。结论 GQQ-ZML-2是一种新型的具有选择性抑制H358细胞增殖作用的海洋来源化合物,其作用机制可能与诱导KRASG12C依赖的ROS增加相关。本文研究为开发新型的拮抗具有KRASG12C突变肿瘤的药物提供了实验依据。     

用熟悉的语言唤醒对全身麻醉患者苏醒质量的影响

目的 观察用熟悉的语言唤醒对全身麻醉患者苏醒质量的影响.方法 全凭静脉全身麻醉下手术患者100例随机分为观察组和对照组,每组50例.对照组患者围麻醉期给予常规处理.观察组在麻醉苏醒期实施熟悉的语言唤醒:术前1 d通过访视和患者建立融洽关系;术毕,在麻醉药停止使用后,用其熟悉的声音呼唤其姓名,每隔15～20 s呼喊1次,...     

Effects of Forging and Heat Treatment on Martensite Lath,Recrystallization and Mechanical Properties Evolution of 18Ni(250) Maraging Steel

The manufacturing process of maraging steel parts include forging, heat treatment and other technological links, and the strengthening mechanism at different stages is different, which has an important impact on the process design of forgings. To investigate the strengthening behavior of maraging steel, forging experiments with different deformation amounts and heat treatment conditions were carried out, and the microstructural and mechanical properties evolution of 18Ni(250) steel was analyzed. The experimental results show that the size of the martensite lath is affected by multiple factors such as the influence of grain size, recrystallization and martensite substructure fraction. The strengthening mechanism of maraging steel during forging and heat treatment is different. Forging combined with heat treatment can refine grains, and the internal defects of the original material can be better eliminated. The thermal deformation can better play the role of grain refinement compared with cyclic phase transformation, which can improve the plasticity of 18Ni(250) maraging steel.     

Assessing the Association between Important Dietary Habits and Osteoporosis: A Genetic Correlation and Two-Sample Mendelian Randomization Study

Objective: Osteoporosis (OP) is the most common bone disease. The genetic and metabolic factors play important roles in OP development. However, the genetic basis of OP is still elusive. The study aimed to explore the relationships between OP and dietary habits. Methods: This study used large-scale genome-wide association study (GWAS) summary statistics from the UK Biobank to explore potential associations between OP and 143 dietary habits. The GWAS summary data of OP included 9434 self-reported OP cases and 444,941 controls, and the GWAS summary data of the dietary habits included 455,146 participants of European ancestry. Linkage disequilibrium score regression (LDSC) was used to detect the genetic correlations between OP and each of the 143 dietary habits, followed by Mendelian randomization (MR) analysis to further assess the causal relationship between OP and candidate dietary habits identified by LDSC. Results: The LDSC analysis identified seven candidate dietary habits that showed genetic associations with OP including cereal type such as biscuit cereal (coefficient = −0.1693, p value = 0.0183), servings of raw vegetables per day (coefficient = 0.0837, p value = 0.0379), and spirits measured per month (coefficient = 0.115, p value = 0.0353). MR analysis found that OP and PC17 (butter) (odds ratio [OR] = 0.974, 95% confidence interval [CI] = (0.973, 0.976), p value = 0.000970), PC35 (decaffeinated coffee) (OR = 0.985, 95% CI = (0.983, 0.987), p value = 0.00126), PC36 (overall processed meat intake) (OR = 1.035, 95% CI = (1.033, 1.037), p value = 0.000976), PC39 (spirits measured per month) (OR = 1.014, 95% CI = (1.011, 1.015), p value = 0.00153), and servings of raw vegetables per day (OR = 0.978, 95% CI = (0.977, 0.979), p value = 0.000563) were clearly causal. Conclusions: Our findings provide new clues for understanding the genetic mechanisms of OP, which focus on the possible role of dietary habits in OP pathogenesis.     

多层螺旋CT胃成像及2D MPR对胃病的显示

CT技术的进步包括多层螺旋CT的引进以及3D成像软件的开发,2DMPR和CT胃造影在同一组数据内可以获得胃部疾病的完整信息,而传统的方法则需要行胃镜、上消化道造影（UGIS）、内镜超声和CT共同完成。多层螺旋CT可以无创评价胃壁以及胃周病变范围,检测和评价胃的恶性肿瘤以及胃的各种炎性病变。     

传染病研究动态

乙肝病毒抑制干扰素α的信号传导,儿童乙型肝炎疫苗接种后表面抗原的血清转换与母亲乙型-肝炎标志物有关,意大利局部卫生区乙型和丙型肝炎病毒的家庭内传播,乙型肝炎病毒的半减期,日本分离出Clostridium Difficile027突变株     

敲低着丝粒蛋白U对顺铂耐药卵巢癌细胞自我更新、顺铂耐药和Wnt/β-catenin信号活性的抑制作用

目的:探讨下调着丝粒蛋白U(CENPU)对卵巢癌(OC)细胞顺铂耐药的影响,并分析其作用机制.方法:采用Western blotting法检测OC细胞(OVCAR3和SKOV3细胞)和顺铂耐药OC细胞(OVCAR3/DDP和SKOV3/DDP细胞)中CENPU蛋白表达水平.将OVCAR3/DDP和SKOV3/DDP细胞...     

联合检测对地中海贫血实验诊断的应用价值

目的 评价红细胞平均体积（MCV）、红细胞脆性试验和血红蛋白（Hb）电泳在地中海贫血诊断及其产前筛查中的价值。方法 选择2004年至2005年间在我院进行系统产前检查的已被基因诊断证实的210例地中海贫血夫妇的筛查实验结果进行回顾性分析。结果 MCV、红细胞脆性试验及Hb电泳单项检测在地中海贫血产前筛查的灵敏度和特异度分别为97．6％、89．5％、90．0％及76．0％、89．3％、97．3％;MCV与Hb电泳、红细胞脆性试验与Hb电泳2项平行联合检测的灵敏度及特异度分别为100．0％、86．7％及99．0％、89．3％,系列联合检测的灵敏度及特异度分别为89．0％、99．7％及87．1％、100．0％。MCV、红细胞脆性试验及Hb电泳3项平行联合检测的灵敏度及特异度分别为100．0％、79．7％;3项系列联合检测灵敏度及特异度为86．7％、100．0％。经u检验,平行联合检测的灵敏度与各单项检测灵敏度之间、系列联合检测的特异度与各单项检测特异度之间差异有统计学意义（P〈0．05）。结论 MCV、红细胞脆性试验及Hb电泳3项联合检测是地中海贫血诊断及其产前筛查中最理想的试验方法。     

围绝经期综合征患者心率变异性与中医辨证分型关系的探索

目的研究心率变异性(HRV)与围绝经期综合征中医辨证分型的关系,探索中医证的客观化。方法通过对87例围绝经期综合征患者与32例正常对照组HRV的频域分析,观察、分析肝肾阴虚型、阴虚阳亢型、气阴两虚型围绝经期综合征与正常对照组HRV各指标的差异。结果围绝经期综合征患者三型之间TP、VLF、LF的差异显著,总的趋势从肝肾阴虚型、阴虚阳亢型、气阴两虚型依次降低(P<0.05,P<0.01);气阴两虚型较其他两型TP、VLF、LF、HF、LF/HF比值、VLF/HF比值、SD、LFnorm降低,HF、HFnorm升高,差异显著(P<0.05,P<0.01);肝肾阴虚型与正常对照组间HRV差异不显著(P>0.05);结论HRV的部分指标可能为围绝经期综合征中医辨证分型的客观化提供参考。     

针刺治疗假性球麻痹吞咽困难89例

假性球麻痹属于上运动神经元延髓麻痹,常见于两侧半球血管病变,如脑梗死、多发性腔隙性梗塞、脑出血等疾病。临床表现为构音障碍,吞咽困难,饮水呛咳,患者不能进食、进水,出现营养不良。笔者运用针刺治疗假性球麻痹89例,疗效显著,现报道如下。