Protective effect of probucol on liver injury induced by carbon tetrachloride in rats

Aim  

To explore protective effect of probucol on liver injury induced by carbon tetrachloride (CCl₄) in rats.     

玻璃体腔注射曲安奈德术后继发性青光眼的发生率和危险因素分析

目的:分析玻璃体腔注射曲安奈德术后继发性青光眼的发生率和危险因素。方法:对连续收治的256例有玻璃体腔注射曲安奈德适应证的各类眼底病患者实施单次玻璃腔注射4mg/0.1mL曲安奈德手术,观察其术后眼压变化情况。主要适应证包括湿性年龄相关性黄斑变性,其它原因如糖尿病性视网膜病变、视网膜中央静脉阻塞、视网膜分支静脉阻塞,视网膜静脉周围炎等引起的黄斑水肿性疾病。结果:256例256眼经至少3mo的追踪观察随访中,56眼发生继发性青光眼,发病率为21.9%。结论:继发性青光眼与年龄、性别、血压、糖尿病、玻璃体腔注药术的不同适应证、术前是否行白内障手术、是否行光动力治疗、是否行玻璃体切除手术等因素没有相关性。注药前青光眼病史的存在和基础眼压高于19mmHg等情况为术后继发性青光眼的显著危险因素。     

老年人肺动脉高压59例临床分析

目的:调查65岁以上老年人肺动脉高压(PAH)患病情况及肺动脉高压分型.方法:回顾性分析2005-05/2007-05月间我科收治的59例老年肺动脉高压患者临床资料.结果:与左心疾病有关的PAH为93.22%,其中与左房或左室心脏病相关的PAH 48例;与左心瓣膜性心脏病相关的PAH 7例.与肺呼吸道疾病和(或)缺氧有关的PAH为5.08%,慢性阻塞性肺疾病3例,垂体腺瘤肢端肥大症1例.年龄和肺动脉压相关系数为0.29(P>0.05).25例中重度肺动脉高压患者中心功能Ⅲ级12例,Ⅳ级8例,3例患者死亡.结论:高血压病和冠心病等左心疾病是老年PAH最常见类型.重度肺动脉高压提示预后不良.老年PAH患者年龄与肺动脉压无关.     

Role of serum angiopoietin-2 level in screening for esophageal squamous cell cancer and its precursors

Background Angiopoietin-2 (Ang-2) is one of the critical regulators of tumor angiogenesis. Studies have shown a significant correlation of Ang-2 expression to tumor invasion and metastasis in various human cancers, but little is known about the serum Ang-2 (sAng-2) levels in esophageal squamous cell cancer (ESCC) and its precursors. In this study, we aimed to investigate its role in screening for ESCC and its precursors.Methods We carried out a free endoscopic screening in Feicheng City, a high ESCC incidence area in Shandong Province of China. Serum samples were collected as follows: 91 from normal subjects, 44 from patients with esophagitis, 85 from patients with hyperplasia, and 13 from patients with early ESCC. In addition, 28 serum samples were obtained from patients with invasive ESCC undergoing surgery in People’s Hospital of Feicheng City. All the subjects of the five groups were diagnosed by histopathology. The sAng-2 levels were tested and compared, and the diagnostic power in early or/and invasive ESCC was calculated in terms of sensitivity and other parameters.Results The sAng-2 levels were (22.0±5.5), (21.3±3.2), (20.5±3.3), (24.0±5.0), and (29.8±5.0) U/ml in normal, esophagitis, hyperplasia, early ESCC, and invasive ESCC groups respectively. It was significantly higher in early ESCC than inhyperplasia group (P=0.009). The invasive ESCC group showed the highest Ang-2 level among all groups (all P=0.000). The sensitivities of sAng-2 to early and invasive ESCC were 23.1% and 78.6% respectively.Conclusion sAng-2 level is related to carcinogenesis and progression of ESCC, but it can not be used to screen for early ESCC.     

转基因过表达肌浆网钙ATP酶基因治疗慢性心力衰竭的实验研究

目的研究肌浆网钙ATP酶2a(SERCA2a)转基因在非缺血性和缺血性心力衰竭中的治疗价值。方法构建含SERCA2acDNA的重组腺病毒和腺相关病毒载体,制备腹主动脉缩窄和心肌梗死心力衰竭两种心衰大鼠模型,分别采用经膈将病毒液(2×1011病毒颗粒)注入心包腔,和局部心肌注射方法导入病毒,术后10~30d经颈动脉插管至左心室进行血流动力学检测。结果重组腺相关病毒rAAV2-SERCA2a导入主动脉束扎心衰大鼠心包腔后10d、30d其血流动力学与对照治疗组(rAAV2-EGFP)组相比有明显改善,30d左室收缩压(LVSP)、左室内压最大上升速率(+dp/dt)、左室内压最大下降速率(-dp/dt)分别提高57%(94mmHgvs147mmHg)、110%(5350mmHg/svs11225mmHg/s)和99.8%(4198mmHg/svs8390mmHg/s),左室舒张末压(LVEDP)则降低60%(22mmHgvs9mmHg)。转导SERCA2a后,rAAV-SERCA2a导入组在30d时SERCA2a蛋白质表达明显高于心衰组和rAAV2-EGFP导入组,达到对照组大鼠水平。重组腺病毒rAV-SERCA2a导入心肌梗死心力衰竭心衰大鼠心肌后21d血流动力学明显优于rAAV2-EGFP导入组,LVSP、+dp/dt和-dp/dt分别提高28%(86mmHgvs110mmHg)、41%(4272mmHg/svs6026mmHg/s)和71%(2789mmHg/svs4756mmHg/s),LVEDP则降低70%(3.89mmHgvs-5.34mmHg),但未能纠正至对照组水平。结论重组腺相关病毒rAAV2-SERCA2a和腺病毒rAV-SERCA2a,介导SERCA2a在大鼠心肌过表达,可有效改善心衰大鼠模型血流动力学状况。     

大鼠成体心肌细胞的分离和培养

目的: 探索稳定的大鼠成体心肌细胞的分离和培养方法. 方法: 应用主动脉逆行生物酶(5 g/L胰蛋白酶及1 g/L胶原酶)灌流法分离大鼠成体心肌细胞,免疫荧光染色法鉴定分离的心肌细胞,比较有无血清培养对心肌细胞的影响. 结果: 心肌细胞的存活率为95%以上;存活细胞呈杆状,形态完整,长宽比约为4～6∶ 1;无血清培养心肌细胞只能存活1 wk,形态不发生改变;而加血清培养心肌细胞可存活2 wk以上,但超微结构发生了改变,且不同血清浓度对成纤维细胞生长具有不同的作用. 结论: 主动脉逆行生物酶(胶原酶和蛋白酶)灌流法是比较理想的心肌细胞分离方法;血清对成体心肌细胞的培养起着重要作用.     

HA（＋ZrO2＋Y2O3）／Ti6Al4V复合涂层的微结构与生物活性

采用射频磁控溅射法成功制备了HA（＋ZrO2＋Y2O3）／Ti6Al4V生物复合涂层。借助于XRD、SEM、FTIR及AFM等对溅射涂层的相组成、微观形貌和界面结合进行了研究，并以模拟体液试验探讨了涂层的生物活性。实验结果表明：磁控溅射的复合涂层呈非晶态，经过退火处理，可以使其完全转化为晶态；复合涂层的微观表面凹凸不平，并呈现网状结构和较多的孔隙，其孔隙直径约为0．5μm-2μm；涂层的生长模式为层状加岛状生长；复合涂层在模拟体液中浸泡一段时间后，表面覆盖一层新生物质——含有CO^2-3的类骨磷灰石，其晶粒非常小，它与自然骨中无机相的结构成份相似．因此复合涂层具有良好生物相容性和生物活性。     

Alcohol-Related Disorders in Beijing, China: Prevalence, Socio-Demographic Correlates, and Unmet Need for Treatment

Background:  The study aimed to determine the prevalence of alcohol use, episodic heavy drinking, and alcohol dependence and their socio-demographic correlates in Beijing, China.
Methods:  A total of 5,926 subjects were randomly selected in Beijing and interviewed using the Composite International Diagnostic Interview (CIDI 1.0). Data on basic socio-demographic and current major medical conditions were also collected.
Results:  The 12-month prevalence of alcohol use and episodic heavy drinking were 32.5 and 13.8%, respectively. The 12-month and lifetime prevalence of alcohol dependence were 1.7 and 4.3%, respectively. Age above 24 years, male sex, being married and employed, low education level (junior high school, primary school or illiterate), rural residence, and having comorbid psychiatric disorders were all significantly associated with a higher likelihood of alcohol-related disorders. Only 2.4% of the subjects with alcohol dependence were receiving treatment, and a mere 1.4% had sought treatment from mental health professionals.
Conclusions:  Nationwide surveys are urgently needed to further explore the prevalence of alcohol-related disorders in China.