全文获取类型
收费全文 | 32610篇 |
免费 | 3301篇 |
国内免费 | 2262篇 |
专业分类
耳鼻咽喉 | 226篇 |
儿科学 | 351篇 |
妇产科学 | 208篇 |
基础医学 | 3364篇 |
口腔科学 | 488篇 |
临床医学 | 4359篇 |
内科学 | 4688篇 |
皮肤病学 | 321篇 |
神经病学 | 1616篇 |
特种医学 | 1150篇 |
外国民族医学 | 15篇 |
外科学 | 2965篇 |
综合类 | 6429篇 |
现状与发展 | 13篇 |
预防医学 | 2453篇 |
眼科学 | 931篇 |
药学 | 3729篇 |
52篇 | |
中国医学 | 2250篇 |
肿瘤学 | 2565篇 |
出版年
2024年 | 100篇 |
2023年 | 540篇 |
2022年 | 1529篇 |
2021年 | 2099篇 |
2020年 | 1470篇 |
2019年 | 1195篇 |
2018年 | 1313篇 |
2017年 | 1175篇 |
2016年 | 1067篇 |
2015年 | 1674篇 |
2014年 | 2056篇 |
2013年 | 1865篇 |
2012年 | 2753篇 |
2011年 | 2778篇 |
2010年 | 1934篇 |
2009年 | 1581篇 |
2008年 | 1698篇 |
2007年 | 1627篇 |
2006年 | 1565篇 |
2005年 | 1247篇 |
2004年 | 1044篇 |
2003年 | 1152篇 |
2002年 | 871篇 |
2001年 | 749篇 |
2000年 | 565篇 |
1999年 | 552篇 |
1998年 | 282篇 |
1997年 | 327篇 |
1996年 | 243篇 |
1995年 | 207篇 |
1994年 | 170篇 |
1993年 | 93篇 |
1992年 | 120篇 |
1991年 | 113篇 |
1990年 | 88篇 |
1989年 | 66篇 |
1988年 | 74篇 |
1987年 | 62篇 |
1986年 | 52篇 |
1985年 | 32篇 |
1984年 | 17篇 |
1983年 | 13篇 |
1982年 | 6篇 |
1981年 | 3篇 |
1980年 | 3篇 |
1979年 | 3篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
51.
Different applications of polymerases with and without proofreading activity in single-nucleotide polymorphism analysis 总被引:2,自引:0,他引:2
Zhang J Li K Liao D Pardinas JR Chen L Zhang X 《Laboratory investigation; a journal of technical methods and pathology》2003,83(8):1147-1154
With the completion of the human genome project, single-nucleotide polymorphisms (SNPs) have become the focus of intense study in biomedical research. Polymerase-mediated primer extension has been employed in a variety of SNP assays. However, these SNP assays using polymerase without proofreading function are compromised by their low reliability. Using a newly developed short amplicon harboring restriction enzyme site, EcoR-I, we were able to compare the single-base discrimination abilities of polymerases with and without proofreading function in primer extension in a broad range of annealing temperatures. Thermodynamic analysis demonstrated a striking single-nucleotide discrimination ability of polymerases with proofreading function. Using unmodified 3'-end allele-specific primers, only template-dependent products were generated by polymerase with proofreading activity. This powerful single-base discrimination ability of exo(+) polymerases was further evaluated in primer extension using three types of 3' terminally modified allele-specific primers. As compared with the poor fidelity in primer extension of polymerases lacking 3' exonuclease activity, this study provides convincing evidence that the use of proofreading polymerases in combination with 3'-end modified allele-specific primers can be a powerful new strategy for the development of SNP assays. 相似文献
52.
The effects of repetitive activation upon voltage-dependent calcium currents (ICa) and transmitter release were studied in dissociated cell cultures of fetal mouse spinal cord and dorsal root ganglion. Sodium and potassium currents were suppressed with tetrodotoxin (TTX) and tetraethylammonium (TEA) ions, 4-aminopyridine (4-AP), and cesium sulfate. Calcium currents were compared under voltage clamp before and after a series of depolarizing clamp pulses in spinal cord (SC) and dorsal root ganglion (DRG) neurons. Repetitive activation resulted in an exponential decline in ICa, with the decrease in ICa being much more marked in DRG compared with SC neurons. Both voltage-dependent inactivation and inactivation related to the intracellular movement of Ca2+ appeared to be involved in the decrement in ICa with repetitive activation. A decrease in transmitter output occurred with repetitive activation in DRG neurons but not in SC neurons (either excitatory or inhibitory). DRG neuron synaptic boutons had fewer mitochondria than did the boutons of either excitatory or inhibitory of SC neurons. The decrement in both ICa and synaptic transmitter output in DRG neurons could last for prolonged periods (at least minutes) following repetitive activation. We hypothesize that this vulnerability of DRG neurons to repetitive activation may be related, at least in part, to a relative incapacity to maintain a low intracellular calcium ion concentration [Ca]i during periods of increased calcium ingress associated with excitation. Such an incapacity to buffer [Ca]i may be one mechanism leading to the inactive synapses seen in some studies in vitro and in vivo of synaptic transmission. 相似文献
53.
54.
N Tsai Z X Gao C L Xu K Zhu G B Yin H Z Shi Y C Jia 《Annales médico-psychologiques》1987,145(7):595-597
The concept and taxonomy of periodic psychosis still remains in controversy for the past 100 years. In China, it was first reported by Ji, and thereafter various rinds of terminology and views were given by different authors. In the presentation, clinical investigation and follow up were carried out in 62 cases. In many of these cases, the so-called "periodic psychosis" or "phasophrenia" was early clinical features of schizophrenia or manic-depression. In those cases with apparent symptoms and signs of diencephalic function, differential diagnosis should be carried out from organic brain syndrome. In a small number of cases, their clinical manifestations, conforming to previous diagnostic criteria, might be an uncommon syndrome. It was concluded by the authors that until now periodic could be considered only as a clinical syndrome rather than a disease entity. 相似文献
55.
Bolun Cheng Xiaomeng Chu Xuena Yang Yan Wen Yumeng Jia Chujun Liang Yao Yao Jing Ye Shiqiang Cheng Li Liu Cuiyan Wu Feng Zhang 《Nutrients》2021,13(4)
Dietary habits have considerable impact on brain development and mental health. Despite long-standing interest in the association of dietary habits with mental health, few population-based studies of dietary habits have assessed depression and fluid intelligence. Our aim is to investigate the association of dietary habits with depression and fluid intelligence. In total, 814 independent loci were utilized to calculate the individual polygenic risk score (PRS) for 143 dietary habit-related traits. The individual genotype data were obtained from the UK Biobank cohort. Regression analyses were then conducted to evaluate the association of dietary habits with depression and fluid intelligence, respectively. PLINK 2.0 was utilized to detect the single nucleotide polymorphism (SNP) × dietary habit interaction effect on the risks of depression and fluid intelligence. We detected 22 common dietary habit-related traits shared by depression and fluid intelligence, such as red wine glasses per month, and overall alcohol intake. For interaction analysis, we detected that OLFM1 interacted with champagne/white wine in depression, while SYNPO2 interacted with coffee type in fluid intelligence. Our study results provide novel useful information for understanding how eating habits affect the fluid intelligence and depression. 相似文献
56.
Chen Yao Zhong Qinyi Luo Jiaxin Tang Yujia Li Mingshu Lin Qian Willey James Allen Chen Jyu-Lin Whittemore Robin Guo Jia 《Prevention science》2022,23(7):1156-1168
Prevention Science - This study aimed to evaluate the efficacy of an intensive lifestyle modification program tailored to rural Chinese women with prior gestational diabetes mellitus compared with... 相似文献
57.
目的调查少数民族地区青少年对新冠疫情的认知及应对情况。方法采用分层抽样的方法,通过“问卷星”在线平台对香格里拉市1949名在校学生进行调查。结果结果显示,81.94%的学生在2020年1月初之前已知晓疫情;83.12%的学生通过电视和微信知晓疫情;9333%的学生认为病毒通过飞沫传播;94.80%的学生会通过戴口罩、不聚餐等方式进行防护;疫情相关知识的正确率为71.44%。不同年龄、性别、民族、专业、生源地、家庭年收入的学生对部分调查问题的差异有统计学意义(P<0.05)。结论学生对疫情认知整体情况较好,部分问题仍有较大提升空间。学校应做好疫情常态化防控,完善心理疏导体系,多措并举,引导学生做好自我防护。 相似文献
58.
59.
Obesityisassociatedwithinsulinresis tance (IR) ,whichplaysacriticalroleinthedevelopmentoftype 2diabetesmellitus.ThemechanismofIRisrelatedtotheabnormalitiesofinsulinreceptor,pre receptor,post recep tor ,resultingindefectivemetabolicpathwayofglucose,lipidand pr… 相似文献
60.
Yi Lu Jia Tian Shangren Wang Xiao Wang Yuxuan Song Kang Liu Kechong Zhou Yongjiao Yang Xiaoqiang Liu 《Andrologia》2021,53(5):e14038
The study was designed and conducted to evaluate the plant-based diet status and its association with erectile function (EF) in Chinese young healthy men. From July 2018 to May 2020, 116 objectively proved physically and psychologically healthy men were selected. Clinical questionnaires, detailed physical examinations and blood tests were all assessed. An overall plant-based diet index (PDI) and a healthful plant-based diet index (hPDI) were developed from each participant to measure plant-based diet quantitatively. The EF was measured by both the International Index of Erectile Function-5 (IIEF-5) and the surrogated measures of endothelial function, peripheral levels of nitric oxide (NO) and E-selectin. In subjects, the mean PDI and hPDI were 53.8 ± 8.2 and 54.7 ± 8.9 respectively. Results of multivariate analysis showed that both PDI and hPDI were negatively associated with BMI (all p < .05), but had no relationships with NO, E-selectin, TT levels and IIEF-5 scores (all p > .05). In a subgroup of male population, Chinese young healthy men, the plant-based diet, measured by PDI and hPDI, was not related to erectile function. The population can adhere to plant-based diet to keep healthy without concerns of negative influence on erectile function. 相似文献