超声弹性成像诊断乳腺局灶性病变价值的初步探讨

目的探讨超声弹性成像对乳腺局灶性病变鉴别诊断的价值。方法应用超声弹性成像对219例患者共290个乳腺结节进行检查,采用5分评分法进行评价。所有患者均行手术经病理证实。结果良性153例共218个结节,恶性66例共72个结节,超声弹性成像诊断恶性病变敏感性为81．9％,特异性为95．0％,准确性为91．7％。结论超声弹性成像有助于乳腺肿瘤良恶性鉴别。     

带旋髂深血管蒂髂骨瓣移植治疗胫骨骨不连

胫骨骨不连,特别是胫骨中下段骨不连临床上较常见,治疗较困难。2000年3月~2005年3月,我们采用带旋髂深血管蒂髂骨瓣移植治疗胫骨骨不连52例,取得了较好疗效。1材料与方法1.1病例资料本组52例,男39例,女13例,年龄17~62岁。胫骨中段骨折14例,下段骨折38例。开放性骨折47例,闭合性骨折5例。粉碎性骨折38例,伴有骨缺损者19例。首次内固定采用钢板11例,交锁髓内钉内固定28例,外固定架固定9例,双矩形钉内固定3例,管形石膏1例。经二次手术内固定16例。病程6~21个月。1.2治疗方法手术在硬膜外麻醉下分两组进行。先将需修复部位的瘢痕组织切除,彻底切…     

复方小柴胡汤对荷瘤小鼠NK细胞功能的影响

目的 探讨复方小柴胡汤(XCHT)对EAC荷瘤小鼠的免疫功能的影响.方法 用3种不同浓度的复方小柴胡汤对EAC荷瘤小鼠灌胃10 d,观察其肿瘤生长情况以及用乳酸脱氢酶释放法检测NK细胞的活性.结果 高、中和低浓度组NK细胞活性分别为(44.21±3.10)%,(51.09±4.94)%和(37.30±2.02)%,与空白对照组(31.26±2.79)%比较,NK细胞活性升高(P＜0.05),而阴性对照组(26.90±1.84)%NK细胞活性显著降低(P＜0.05);高、中和低浓度组的肿瘤质量为(300.6±48.9)mg,(226.5±36.4)mg和(445.9±60.2)mg,与阴性对照组(661.5±102.8)mg比较,均能抑制肿瘤的生长(P＜0.05),其中以中浓度组效果最为明显.结论 经过复方小柴胡汤灌胃的EAC荷瘤小鼠NK细胞活性均升高,3种不同浓度的复方小柴胡汤均使肿瘤生长减慢.     

Efficient Use of Graphene Oxide in Layered Cement Mortar

Graphene oxide (GO) has been found to be an attractive nanomaterial to improve the properties of cementitious composites. However, the use of GO in the industry is limited by its high cost. To achieve a higher cost/performance ratio, GO can be strategically applied in certain parts of cementitious composites structure according to the principle of functionally graded materials. In this study, graded distribution of GO in cement mortar was achieved by sequentially casting a fresh GO-incorporated cement layer on another cement mortar layer. The mechanical properties, especially flexural strength, of layered cement mortar were found to be dependent on the GO content, the delay time, and the interface formed due to layering fabrication. With the GO incorporated in the tensile region only (30% of the total depth), the flexural strength of the layered beam attained 90.91% of that of the beam, with GO uniformly distributed throughout the sample. Based on the results of rapid chloride migration tests, when 12 mm GO-incorporated cement mortar layer was used, the chloride migration coefficient was reduced by 21.45%. It was also found that the measured chloride migration coefficient of layered cement mortar agreed with the series model. The present investigation provides an efficient approach to use GO in cement-based materials from the perspective of mechanical and durability properties.     

利多卡因对缺氧/复氧后肝细胞Bcl-2、Caspase-3蛋白表达及细胞凋亡的影响

目的探讨利多卡因预处理对肝细胞缺氧/复氧后Bcl-2、Caspase-3蛋白表达及细胞凋亡的影响。方法将体外培养的肝细胞分为三组:缺氧/复氧组(Ⅰ组)、利多卡因预处理组(Ⅱ组)和正常对照组(Ⅲ组),每组10份。检测肝细胞缺氧/复氧培养后细胞培养液中谷丙转氨酶(ALT)、谷草转氨酶(AST)浓度,肝细胞Bcl-2、Caspase-3蛋白表达、肝细胞凋亡率及超微结构变化。结果Ⅰ、Ⅱ组细胞培养液中ALT、AST浓度、肝细胞Caspase-3蛋白表达和肝细胞凋亡率较Ⅲ组均升高(P<0.05),透射电镜示肝细胞损伤,可见凋亡细胞;Ⅱ组细胞复氧后培养液中ALT、AST浓度、肝细胞Caspase-3蛋白表达和细胞凋亡率均低于Ⅰ组(P<0.05),而肝细胞Bcl-2蛋白表达较Ⅰ组升高(P<0.05),透射电镜观察也显示Ⅱ组肝细胞比Ⅰ组损伤轻微。结论利多卡因预处理可以在一定程度上减轻缺氧/复氧后肝细胞损伤,降低细胞凋亡率,保护机制可能与Bcl-2、Caspase-3蛋白表达有关。     

原发性阴道恶性黑色素瘤患者预后影响因素分析:32例病例报道及系统评价

目的 探讨原发性阴道恶性黑色素瘤(PMMV)的预后及其影响因素.方法 收集郑州大学第一附属医院2013年1月至2018年6月收治的32例和中英文文献报道69例PMMV患者的临床病理资料,汇总后系统评价PMMV患者临床病理特征、预后及其影响因素.结果 共纳入101例PMMV患者.平均发病年龄61岁,79.2％为绝经后女性...     

含毒性中药饮片中成药的使用分析及管理

目的:分析上海中医药大学附属市中医医院(以下简称“本院”)含毒性中药饮片中成药的使用情况,为医疗机构合理管理含毒性中药饮片中成药提供参考.方法:统计本院含毒性中药饮片中成药的品种,对其说明书、所含毒性饮片的类型、毒性大小以及各科室使用情况等进行系统分析.结果:本院对于含毒性中药饮片中成药的临床应用仍存在不合理用药现象....     

护理干预对中青年慢性乙型肝炎患者生活方式的影响

目的 探讨护理干预对中青年慢性乙型肝炎(CHB)患者生活方式的影响.方法 将322例患者按就诊顺序分为观察组和对照组各161例.在不改变原治疗方案的前提下,观察组分别在饮食、休息、烟酒、运动、情绪等方面进行有计划、有针对性的护理干预措施,对照组则给予常规健康教育.比较两组生活方式、肝功能的转变情况.结果 观察组干预后生...     

Identification of FDA-Approved Drugs and Bioactives that Protect Hair Cells in the Zebrafish (Danio rerio) Lateral Line and Mouse (Mus musculus) Utricle

The hair cells of the larval zebrafish lateral line provide a useful preparation in which to study hair cell death and to screen for genes and small molecules that modulate hair cell toxicity. We recently reported preliminary results from screening a small-molecule library for compounds that inhibit aminoglycoside-induced hair cell death. To potentially reduce the time required for development of drugs and drug combinations that can be clinically useful, we screened a library of 1,040 FDA-approved drugs and bioactive compounds (NINDS Custom Collection II). Seven compounds that protect against neomycin-induced hair cell death were identified. Four of the seven drugs inhibited aminoglycoside uptake, based on Texas-Red-conjugated gentamicin uptake. The activities of two of the remaining three drugs were evaluated using an in vitro adult mouse utricle preparation. One drug, 9-amino-1,2,3,4-tetrahydroacridine (tacrine) demonstrated conserved protective effects in the mouse utricle. These results demonstrate that the zebrafish lateral line can be used to screen successfully for drugs within a library of FDA-approved drugs and bioactives that inhibit hair cell death in the mammalian inner ear and identify tacrine as a promising protective drug for future studies.     

Coexistence of acquired hemophilia A and epidermolysis bullosa acquisita: Two case reports and published work review

Epidermolysis bullosa acquisita (EBA) is a rare chronic subepidermal bullous autoimmune disease. The occurrence of acquired hemophilia A (AHA) is low and so the coexistence of EBA and AHA is extremely rare. We herein described a case of EBA coexisting with AHA and a case of EBA coexisting with AHA and hepatitis B. These EBA may be related to the pathogenesis of AHA. In this study, we analyzed the clinical features in the two Chinese cases of EBA coexisting with AHA, and found esophageal hemorrhage and hematemesis were the main symptoms of both patients. Cyclosporin, prednisone and lamivudine effectively control EBA with AHA and hepatitis B. The dose of cyclosporin should be more than 4 mg/kg per day and the period of treatment should be longer than 5 months to reduce the risk of EBA co‐occurring with AHA.