Suppressive effect of immature erythroid cells on the B-cell proliferation

Immature erythroid cells suppress the proliferative response of preactivated B lymphocytes to lipopolysaccharide. The same effect is observed when recombinant human interleukin-2 or culture medium conditioned by preactivated T or B cells is added to cultured cells. Suppressive nucleate erythroid cells are resistant to leucine methyl ester. Translated fromByulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 123, No. 1, pp. 66–70, January, 1997     

连续核移植对异种山羊(Bore)克隆胚胎发育的影响

目的探讨连续核移植与异种山羊克隆胚胎发育之间的关系。方法分别以波尔(Bore)山羊耳部成纤维细胞、山羊-兔异种克隆桑椹胚卵裂球为核供体,以兔卵母细胞为受体,进行连续核移植。结果共构建145枚异种原代重构卵、73枚继Ⅰ代及20枚继II代重构卵,经电融合后,获得重构胚数分别为90、58和14枚,融合率分别为62.1%、79.5%和70%;162枚重构胚在同等条件下进行体外共培养,卵裂率分别为72.2%、75.9%和28.6%,囊胚率分别为10%、13.8%和0%;融合率方面,原代、继I及继II重构卵之间无显著差异(P>0.05);早期发育率无显著差异,但继Ⅰ代重构胚高于原代重构胚,相反,继II代远低于前两者,差异极显著(P<0.01)。结论波尔山羊体细胞核经第1次核移植过程中异种受体卵胞质作用后,比高度分化的体细胞更有利于重构胚的发育,但异种山羊克隆胚卵裂球反复多次暴露在异种卵胞质中,可能不利于细胞核的发育。     

The significance of a rapid cold hemagglutination test for detecting mycoplasma infections in children with asthma exacerbation.

BACKGROUND AND PURPOSE: Mycoplasma pneumoniae infection is a frequent cause of community-acquired respiratory infections in children and adults. However, standardized, rapid, specific methods for its diagnosis are lacking. The relationship between M. pneumoniae infection and asthma exacerbation has been recently discussed in the literature. We investigated the accuracy of rapid detection of mycoplasma infection by cold hemagglutination test compared to conventional enzyme immunoassays. The clinical characteristics of mycoplasma infection seen during emergent visits in asthmatic children were reviewed. METHODS: We retrospectively reviewed medical records of patients with asthma exacerbation visiting the Department of Pediatric Emergency, National Taiwan University Hospital, over a 12-month period. Subjects 2-18 years of age diagnosed with asthma at our outpatient clinic were included in this study. Patients with immunodeficiency, congenital anomalies, neurological diseases and irregular follow-up were excluded. RESULTS: A total of 269 children (174 males and 95 females) with a mean (+/- standard deviation) age of 6.15 +/- 3.08 years were included. The prevalence of asthma exacerbation in regular follow-up patients was 13.4%, and as many as 19.6% of cases (74/378 person-times) required hospitalization. Asthma attacks were most prevalent during December. 126 patients had both rapid cold hemagglutination testing and mycoplasma immunoglobulin M titers determined using acute blood samples drawn in the emergency room; 46 (36.5%) of these patients demonstrated mycoplasma infection. Sensitivity and specificity of the rapid cold hemagglutination test was 78.3% and 41.3%, respectively. The positive predictive value was 43.4%. Comparison of patients with or without mycoplasma infection revealed no differences in gender, age, chest X-ray findings, and most symptoms/signs and laboratory data, except that more signs of fever and auscultatory rales were seen in the non-mycoplasma infection group. CONCLUSIONS: Mycoplasma infections could be an exacerbating factor for asthma, and the rapid cold hemagglutination test should not be a guideline for prescribing macrolides in the emergency room.     

Monitoring the level of radioactive contamination of surfaces by sources of gamma-radiation in departments of radionuclide diagnosis

Oncology Research Center, Russian Academy of Medical Sciences, Moscow. Translated from Meditsinskaya Tekhnika, No. 6, pp. 9–10, November–December, 1992.     

Role of glycolysis in the genesis of early postocclusion arrhythmias

Antiarrhythmic effects of phosphorylated glycolysis intermediates glucose-1-phosphate, fructose-1,6-diphosphate, and phosphoenolpyruvate in early postocclusion arrhythmias are shown in rat experiments; in contrast to these, D-glucose, sodium pyruvate, and monoiodacetate, a glycolysis inhibitor, exert no appreciable antiarrhythmic effect. Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 117, N ^o 6, pp. 625–626, June, 1994 Presented by P. V. Sergeev, Member of the Russian Academy of Medical Sciences.     

Methodology of functional studies in nephrologic clinic

Authors have worked out the methodology of functional diagnosis in nephrologic clinic—strategy, tactics and individual programme—on the basis of their own experience. The strategy is based on the natural functions of excretory and incretory renal function and quantitative and qualitative characteristics of indices. The tactics is based on the natural functions of evolution of renal diseases and probable exogenic and endogenic influences. The individual program involves standardization of conditions, complex usage of methods and integration of the results obtained. The interrelation between the methodologic foundations and their components is presented.     

伊柔美白霜对家兔皮肤祛斑效果的观察

目的考察伊柔美白霜的祛斑功效。方法在家兔皮肤上涂抹补骨脂酊，待日光照射使皮肤上形成黄褐斑后，涂抹伊柔美白霜。结果涂抹伊柔美白霜20d后，黄褐斑有明显减退。结论伊柔美白霜具有脱色、增白的功效。