盐酸多奈哌齐对血管性痴呆小鼠海马ERK2及p-ERK表达变化的影响

目的 探讨盐酸多奈哌齐对血管性痴呆(VD)小鼠海马中细胞外信号调节激酶(ERK2、p-ERK)表达变化的影响.方法 采用双侧颈总动脉反复缺血-再灌注法制备小鼠VD模型.将小鼠随机分为对照组、模型组及药物组,药物组给予盐酸多奈哌齐灌胃治疗.术后第29、30天,经跳台试验和水迷宫试验对各组小鼠进行行为学成绩测试,用免疫组化方法观察各组小鼠海马CA1区ERK2及海马CA3区p.ERK的表达变化.结果 盐酸多奈哌齐明显改善了VD小鼠学习、记忆成绩(P＜0.05).模型组海马CA1区ERK2的阳性细胞数目较对照组,药物组减少(P＜0.05),阳性细胞平均光密度值亦较对照组、药物组降低(P＜0.05);CA3区p-ERK阳性部位平均光密度值较对照组、药物组降低(P＜0.05).而药物组与对照组间上述变化无显著性差异(P＞0.05).结论 ERK2的表达减少可能参与了VD的发病机制,盐酸多奈哌齐通过增加乙酰胆碱含量,后者作用于毒蕈碱乙酰胆碱受体(M受体)激活ERK(p-ERK),有助于改善学习和记忆功能.     

Sorafenib enhances effects of transarterial chemoembolization for hepatocellular carcinoma: a systematic review and meta-analysis

Background and aim

Combination therapy of sorafenib and transarterial chemoembolization (TACE) showed benefits for hepatocellular carcinoma (HCC). This systematic review aims for evaluation of efficacy and safety between sorafenib plus TACE and TACE alone for HCC.

Methods

We systematically searched multi-databases to identify eligible studies. Studies comparing sorafenib combined with TACE and TACE alone for HCC were included.

Results

Nine studies with 900 patients (sorafenib + TACE = 446, TACE = 454) were finally included. Sorafenib combined with TACE significantly reduced 6-month mortality [OR 0.24, 95 % confidential interval (CI) 0.09–0.68, P = 0.007] and 1-year mortality (OR 0.35, 95 % CI 0.21–0.56, P < 0.0001), but did not decrease 2-year mortality (OR 0.58, 95 % CI 0.14–2.46, P = 0.46). Although combination therapy tend to reduce 3-month (OR 0.76, 95 % CI 0.52–1.10, P = 0.15) and 6-month progression free rate (OR 0.27, 95 % CI 0.07–1.05, P = 0.06), the changes were not significant. Additionally, objective response ratio (OR 0.39, 95 % CI 0.19–0.78, P = 0.008) and clinical benefit ratio (OR 0.27, 95 % CI 0.15–0.50, P < 0.0001) also favored for combination therapy, which, however, caused higher morbidity, especially hand-foot skin reaction (OR 53.71, 95 % CI 28.86–99.93, P < 0.00001), hematological events (OR 14.8, 95 % CI 6.07–36.07, P < 0.00001), diarrhea (OR 6.62, 95 % CI 3.82–11.45, P < 0.00001), hypertension (OR 5.03, 95 % CI 3.02–8.38, P < 0.00001), rash/desquamation (OR 5.67, 95 % CI 3.58–8.99, P < 0.00001), and fatigue (OR 2.5, 95 % CI 1.09–5.72, P = 0.03).

Conclusion

Combination of sorafenib and TACE showed survival and clinical benefits in patients with HCC, though enhanced morbidity.     

miR-96调控MYB表达与儿童急性髓系白血病细胞侵袭及凋亡活性相关性分析

目的:分析儿童急性髓系白血病(AML)血清miR-96靶向调控沉默转录因子MYB表达水平与侵袭及凋亡活性的相关性。方法:选择2017年1月至2019年11月中国人民解放军联勤保障部队第九二八医院确诊AML患儿共65例(其中35例初发,30例完全缓解),同时选择30例免疫性血小板减少患儿作为对照组。分析两组患儿的临床特征。qRT-PCR检测并比较两组患儿外周血标本中miR-96与MYB的表达水平;通过脂质体Lipofectamine2000介导将miR-96 mimics及其阴性对照(NC)、inhibitor-miR-96及其NC转染至4组HL60细胞,采用Western blot检测各组细胞MYB表达水平;采用Transwell法检测细胞的侵袭能力;分别在24、48和72 h采用四甲基偶氮唑蓝(MTT)细胞增殖试验检测细胞增殖活性;采用流式细胞术检测细胞凋亡率。结果:与对照组比较,AML患儿miR-96呈高表达,而MYB呈低表达(P<0.05)。与完全缓解AML患儿相比,初发AML患儿miR-96高表达,MYB呈低表达(P<0.05)。Western blot检测显示,miR-96转染后4组HL60细胞MYB的表达水平存在差异(P<0.05),miR-96 mimics组最低,miR-96 NC组与inhibitor-miR-96 NC组次之,inhibitor-miR-96组最高(P<0.05),而miR-96 NC组与inhibitor-miR-96 NC组无差异(P>0.05)。Transwell法检测显示,miR-96过表达对HL60细胞侵袭具有促进效应。MTT细胞增殖试验显示,miR-96促进HL60细胞增殖,抑制凋亡,具有时间依赖性(r=0.804)。流式细胞术检测结果显示,miR-96抑制HL60细胞凋亡作用。结论:miR-96通过靶向负调控MYB表达水平影响AML细胞侵袭及凋亡水平,可能成为治疗儿童AML的一个新策略。     

两年新兵基础训练中应力性骨折的前瞻性研究

为查明我军新兵应力性骨折（ＳＦ）的发生情况，并为致伤因素和干预措施研究提供基础，作通过两个年度的前瞻性研究，得出我军某甲种步军师新兵基础训练期间ＳＦ的发生率为３４％或１２人次／（１００人．月）步兵分队的发生率最高（３９．２％），炮兵分队（２３．５％）又高于勤务分队（１２．７％），ＳＦ单位分布状况与各单位的实际训练强度成正比，两年度ＳＦ时间分布特征不同，因训练计划的变化而变化，ＳＦ可见于下肢各骨，     

Acute Toxicity of a Novel anti-fouling Material Additive DCOIT to Marine Chlorella sp

DCOIT (4,5-dichloro-2-n-octyl-4-isothiazolin-3-one) is the main ingredient in SeaNine-211, a new antifouling agent that replaces organotin compounds to prevent the growth of fouling organisms on board. Biocides from antifoulants can cause problems for marine ecosystems by destroying non-target algal species. This study evaluated the potential adverse effects DCOIT using the Marine Chlorella sp. The concentration of DCOIT were set according to the semi-inhibitory concentrations for acute exposure experiments, and relevant oxidative stress indicators were measured to assess the acute toxic effects. The results showed that the inhibition concentrations (IC50) of DCOIT against Marine Chlorella sp was 2.522 mg/L. The genes related to photosynthesis and antioxidant capacity showed the effect of promoting low concentration and inhibiting high concentration. In addition, based on the ultrastructural observation and the expression analysis of photosynthesis related genes, it was found that DCOIT had a significant effect on plant photosynthesis.

     

Human development index is associated with mortality-to-incidence ratios of gastrointestinal cancers

AIM:To identify the role of human development in the incidence and mortality rates of gastrointestinal cancers worldwide.METHODS:The age-standardized incidence and mortality rates for gastrointestinal cancers,including cancers of the esophagus,stomach,pancreas,liver,gallbladder,and colorectum,were obtained from the GLOBOCAN 2008 database and United States Cancer Statistics(USCS)report.The human development index(HDI)data were calculated according to the 2011 Human Development Report.We estimated the mortality-toincidence ratios(MIRs)at the regional and national levels,and explored the association of the MIR with development levels as measured by the HDI using a modified"drug dose to inhibition response"model.Furthermore,countries were divided into four groups according to the HDI distribution,and the MIRs of the four HDI groups were compared by one-way ANOVA followed by the Tukey-Kramer post-hoc test.Statespecific MIRs in the United States were predicted from the estimated HDI using the fitted non-linear model,and were compared with the actual MIRs calculated from data in the USCS report.RESULTS:The worldwide incidence and mortality rates of gastrointestinal cancers were as high as 39.4and 54.9 cases per 100000 individuals,respectively.Linear and non-linear regression analyses revealed an inverse correlation between the MIR of gastrointestinal cancers and the HDI at the regional and national levels(<0;P=0.0028 for regional level and<0.0001 for national level,ANOVA).The MIR differed significantly among the four HDI areas(very high HDI,0.620±0.033;high HDI,0.807±0.018;medium HDI,0.857±0.021;low HDI,0.953±0.011;P<0.001,oneway ANOVA).Prediction of the MIRs for individual United States states using best-fitted non-linear models showed little deviation from the actual MIRs in the United States.Except for 28 data points(9.93%of282),the actual MIRs of all gastrointestinal cancers were mostly located in the prediction intervals via the best-fit non-linear regression models.CONCLUSION:The inverse correlation     

A new active compound against kidney deficiency from the fruits of Rubus corchorifolius

Constituents of the fruits of Rubus corchorifolius were investigated. A new compound, namely rubusin A (1), along with three known compounds, was isolated and characterized. Among them, the new compound exhibited significant activity against kidney deficiency, and quercetin and kaempferol were isolated from the fruits of R. corchorifolius for the first time.     

腹腔镜辅助阴式子宫切除37例临床分析

目的通过对腹腔镜辅助阴式子宫切除术（LAVH）和经腹子宫切除术（TAH）两种方法的比较,探讨腹腔镜辅助阴式子宫切除术（LAVH）的优越性。方法回顾性分析2008年1月-2009年6月在妇科施行LAVH和TAH的病例,对术后恢复情况进行比较。结果术中出血量LAVH组（103.75±46.73）ml比TAH组（181.72±54.37）ml明显减少（P〈0.05）。术后肛门排气时间LAVH组（20.48±6.20）h比TAH组（30.26±11.41）h明显缩短（P〈0.05）。术后病率LAVH组比TAH组明显减少（P〈0.05）。术后住院时间LAVH组（4.95±1.21）d比TAH组（7.18±2.62）d明显缩短（P〈0.05）。两种术式的手术时间差异无统计学意义（P〉0.05）。结论 LAVH具有创伤小、术中出血少、术后病率低、恢复快等优点,值得推广。     

急性电刺激疑核对TNBS诱导的IBD大鼠IL-6 mRNA表达的影响

目的:探讨疑核对炎症性肠病(inflammatory bowel disease,IBD)相关细胞因子IL-6的调控作用,以及在IBD发生发展过程中发挥的作用.方法:三硝基苯磺酸(TNBS)诱导IBD大鼠动物模型,采用脑立体定位技术和电生理方法,应用Real-time PCR方法来检测急性电刺激疑核前后IBD大鼠结肠、胸腺、脾脏、外周血淋巴细胞中IL-6 mRNA表达水平的变化.结果:经急性电刺激疑核后,IBD模型大鼠结肠、胸腺、脾脏及外周血淋巴细胞中IL-6的mRNA转录水平均显著升高(3.26E-02±5.83E-03 vs 5.12E-03±6.86E-04,5.52E-02±9.56E-03 vs 2.04E-02±3.58E-03,3.42E-02±7.19E-03 vs 2.91E-03±3.41E-04,8.12E-03±4.24E-04 vs 3.74E-03±5.73E-04,均P<0.01).而假刺激组和对照组没有明显变化(P>0.05).结论:疑核对IBD大鼠相关细胞因子IL-6具有一定的调控作用,且以正调控作用为主,并且在IBD发生发展过程中可能发挥着重要的作用.