重组人可溶性DR5蛋白的分离纯化及其生物活性检测

目的:提纯毕赤酵母GS115菌株表达的重组人可溶性死亡受体5(sDR5)蛋白,并研究其对肿瘤坏死因子相关凋亡诱导配体(TRAIL)所诱导细胞的阻断效应。方法:大量扩增可溶性DR5酵母表达载体转化阳性的毕赤酵母GS115菌株,收集上清,利用Ni-NTA Agarose亲和层析柱纯化获得重组可溶性DR5,用SDS-PAGE检测纯化产物的纯度。用流式细胞仪检测纯化的可溶性DR5对TRAIL诱导Jurkat细胞凋亡作用的阻断效应。结果:转化阳性的毕赤酵母GS115菌株可以成功表达分子量为23kD的可溶性DR5蛋白,经过Ni-NTAAgarose亲和层析柱纯化,SDS-PAGE鉴定显示获得了高纯度的重组人可溶性DR5蛋白,培养上清产量达28.69mg/L;体外活性检测结果显示,纯化的可溶性DR5蛋白几乎完全可以阻断TRAIL诱导的Jurkat细胞凋亡作用。结论:经纯化后的重组人可溶性DR5蛋白可以有效阻断TRAIL诱导的细胞凋亡作用,显示DR5在TRAIL诱导Jurkat细胞凋亡作用中起着十分关键的作用。     

三参调胃汤治疗慢性萎缩性胃炎85例临床观察

慢性萎缩性胃炎是以胃黏膜腺体萎缩为特征的一种常见的消化系统疾病。病程长，易反复发作，病变随年龄增大而有恶变的趋势，因此1978年（WHO）被列为胃癌的癌前状态，在其基础上伴发的不完全肠化生和中、重度异型增生则被视为癌前病变。因此积极治疗慢性萎缩性胃炎，是阻断其向癌发展以减少胃癌发病率的有效手段。笔者根据中医辨证，运用自拟三参调胃汤为基本方治疗慢性萎缩性胃炎，疗效较好。现报道如下。     

多媒体技术在仪器分析教学中的应用

随着科学技术的迅速发展，人类积累的知识量越来越多，知识更新的速度也越来越快，促使教学手段的不断更新。多媒体教学以其课堂信息量大、题材丰富形象、形式活泼多样等特点，在医学院校得到了广泛的普及。仪器分析课作为中医药院校药学专业开设的一门专业基础课，其课程内容繁多，仪器结构复杂，原理抽象，涉及应用面广，使得该课程的学习变得困难，也为教学工作提出了更高要求。多媒体技术的应用则为该课程的学习提供了有利条件。     

慢性肾功能衰竭及血液透析患者脂质代谢紊乱的分析

目探讨慢性肾功能衰竭(CRF)患者及血液透析(HD)患者脂质代谢紊乱的特点。方法慢性肾功能衰竭患者43例和正常人22例,分别取外周肝素抗凝静脉血5ml,送生化实验室检验。CRF患者按病情分期及治疗与否分为:氮质血症组、尿毒症组(未透析)、血液透析组,进行检测TG、CH、HDL、LDL、apoAⅠ、apoB-100,TG、CH采用酶法,HDL、LDL采用直接法,apoAⅠ及apoB-100均采用免疫透射比浊法。结果氮质血症组与尿毒症组(未透析)各项指标盖异不显著,均表现为LDL升高而HDL则降低,与对照组差异显著;血液透析组主要表现为TG和LDL升高,HDL则下降;apoA各组均降低,apoB升高,且与正常对照组之间差异有统计学意义。尿毒症组与血液透析组甘油三酯和高密度脂蛋白差异显著(P＜0．05),CH在各组与时照组之间无差异,无统计学意义。结论慢性肾功能衰竭患者存在脂质代谢紊乱,主要表现为TG、LDL和apoB增高,HDL和apoA降低,而且脂质代谢紊乱与其病情相关性不大,并且血液透析并不能改善脂质代谢紊乱,甚至加重。     

股骨颈骨折几种内固定方法的生物力学研究

为探讨符合生物力学原理的股骨颈骨折的固定方法，为临床应用提供理论基础。用7具成人新鲜股骨上段标本，制成内收型股骨颈头颈型骨折。随机分成4组，A组用2孔动力髋螺钉（danamic hip screw DHS）固定，B组用3枚空心加压螺钉固定（cannulated compression screw CCS），C组用三翼钉（Smith—petersen nail SPN）固定，D组为正常原始骨组（Intact bone IB）。用完整的生物力学实验测定各种固定方法在不同载荷下的载荷．应变值，头下沉位移和偏轴位移，股骨的轴向刚度和扭转刚度，以及应力遮挡率。结果表明，A组的主要生物力学指标如股骨颈张力侧、压力侧载荷．应变值，股骨头水平及下沉载荷．位移曲线，股骨的轴向刚度及应力遮挡率均优于B组和C组。差异有显著性（P〈0.01）。而股骨的扭转刚度测定结果显示，A组和B组无显著差异（P〉0．05）。认为2孔动力髋螺钉固定股骨颈骨折更符合生物力学要求。     

Expression and bioinformatic analysis of lymphoma-associated novel gene KIAA0372

The purpose of this study was to explore the differentially expressed genes in lymph-node cells (LNC) of lymphomas and reactive lymph node hyperplasia, and to perform an initial bioinformatic analysis on a novel gene, KIAA0372, which is highly expressed in the LNC of lymphomas. mRNA extracted from LNC of lymphomas and reactive lymph node hyperplasia were respectively marked with biotin and hybridized with Gene Expression Chips, resulting in differentially expressed genes. Initial bioinformatic analysis was then performed on a novel gene named KIAA0372, whose function has not yet been explored. Its structure and genomic location, its product’s physical and chemical properties, subcellular localization and functional domains, were also predicted. Further, a systematic evolution analysis was performed on similar proteins from among several species. Using Gene Expression Chips, many differentially expressed genes were uncovered. Efficient bioinformatic analysis has fundamentally determined that KIAA0372 is an extracellular protein which may be involved in TGF-β signaling. Microarray is an efficient and high throughput strategy for detection of differentially expressed genes. And KIAA0372 is thought to be a potential target for tumor research using bioinformatic analysis.     

简易胰岛素敏感性指数评估PCOS胰岛素抵抗

目的:寻求一个简单可靠的胰岛素敏感性指数用于评估多囊卵巢综合征(PCOS)患者胰岛素抵抗程度.方法:对47名PCOS患者(按BMI分为肥胖组和非肥胖组)和20名健康女性(正常对照组)行口服葡萄糖耐量试验(OGTT)与Botnia钳夹术.结果:在三组行两两比较Botnia钳夹术获得的M值,均有统计学意义,全部P＜0.05,而肥胖组的M值最低.来源于OGTT的胰岛素抵抗指数SumaI与Botnia钳夹术获得的M值相关性好(r=-0.642,p＜0.001).结论:在评估PCOS患者胰岛素抵抗程度时,SumaI是个比较可靠的简易指数.     

Expressions of leptin and insulin-like growth factor-I are highly correlated and region-specific in adipose tissue of growing rats

OBJECTIVE: Anatomically distinct adipose tissue regions differ in their predominant modality of growth (i.e., cellular hypertrophy vs. hyperplasia). We examined site-specific patterns of expression of two genes whose products, leptin and insulin-like growth factor-I (IGF-I), could be involved in mediating differential growth and metabolism of white adipose tissue. We also related these patterns of expression to measures of adipose depot cellularity. RESEARCH METHODS AND PROCEDURES: Male Wistar rats were fed ad libitum and studied from ages 7 weeks to approximately 12 months. Terminal measures of body weights; weights, composition, and cellularity of four white adipose depots; circulating leptin and IGF-I; and adipose depot-specific expression levels of leptin and IGF-I were measured in subsets of rats at 7, 12, 22, 42, and 46 weeks of age. RESULTS: Both leptin and IGF-I mRNAs are quantitatively expressed in a depot-specific manner, in the following order: retroperitoneal approximately equals epididymal > mesenteric > subcutaneous inguinal. Furthermore, there is a marked correlation between the expressions of these hormones in the various regions of adipose tissue of rats during the first year of life. The mechanisms that underlie the parallel expressions of leptin and IGF-I appear to be related to fat-cell volume. DISCUSSION: Because both leptin and IGF-I have been implicated in the regulation of energy homeostasis and are both expressed in adipose tissue, the depot-specific linkage between the two genes suggests interaction at the autocrine level. This interaction may have an important role in determining functional properties particular to individual adipose depots.     

硝酸甘油与丹参对心脏病患者血液动力学的影响

本文报告２０例心功能均在正常范围的心脏病患者，用Ｓｗａｎ－Ｇａｎｚ导管，对比测定了舌下含硝酸甘油０．３ｍｇ和静脉注射复方丹参注射液４ｍｌ之后，其即刻及１５、３０、４５ｍｉｎ的肺毛细血管嵌楔压（ＰＣＷ）、肺动脉平均压（ＭＰＡＰ）、心脏排血量（ＣＯ）、心排血指数（ＣＩ）、体表平均动脉压（ＳＭＰ）及心率（ＨＲ）的血液动力学变化。发现两种药物都有扩张血管、降低左室充盈压和增加心排血量的作用；但作用出现的时间及持续时间不同，特别是ＣＯ、ＣＩ的增加，丹参明显优于硝酸甘油，作用较持久，值得临床选用。     

中药材金莲花

金莲花为毛莨科金莲花TrolliuschinensisBunge或短辩金莲花T．ldebouriiReichb．等的花。前者生于海拔1000～2000m的山地草坡或疏林下，分布于河北、山西、内蒙南部。短瓣金莲花生于林间草地，分布于黑龙江和内蒙北部，以河北省承德地区的药材质量为佳，初夏时节（5月份）采下，晾干、药用。1化学成分金莲花的主要化学成分有3类。的黄酮类主要是3’，4’，5’一三经基黄酮一7一鼠车精葡萄糖忒，b）生物碱类，。）叶黄素J不氧化合物（xanthoPh－yll－ePoxyd）及金莲花费质（trollixanthin），这2种成分在短瓣金莲花中含有。其抗菌成分主要…