全文获取类型
收费全文 | 509篇 |
免费 | 26篇 |
国内免费 | 24篇 |
专业分类
儿科学 | 19篇 |
妇产科学 | 8篇 |
基础医学 | 54篇 |
口腔科学 | 4篇 |
临床医学 | 69篇 |
内科学 | 102篇 |
皮肤病学 | 2篇 |
神经病学 | 9篇 |
特种医学 | 54篇 |
外科学 | 31篇 |
综合类 | 12篇 |
预防医学 | 11篇 |
眼科学 | 39篇 |
药学 | 129篇 |
中国医学 | 1篇 |
肿瘤学 | 15篇 |
出版年
2019年 | 3篇 |
2018年 | 3篇 |
2017年 | 3篇 |
2016年 | 7篇 |
2015年 | 6篇 |
2014年 | 5篇 |
2013年 | 13篇 |
2012年 | 9篇 |
2011年 | 12篇 |
2010年 | 16篇 |
2009年 | 20篇 |
2008年 | 10篇 |
2007年 | 25篇 |
2006年 | 20篇 |
2005年 | 19篇 |
2004年 | 20篇 |
2003年 | 7篇 |
2002年 | 17篇 |
2001年 | 9篇 |
2000年 | 8篇 |
1999年 | 14篇 |
1998年 | 18篇 |
1997年 | 16篇 |
1996年 | 19篇 |
1995年 | 19篇 |
1994年 | 16篇 |
1993年 | 22篇 |
1992年 | 9篇 |
1991年 | 9篇 |
1990年 | 13篇 |
1989年 | 16篇 |
1988年 | 15篇 |
1987年 | 11篇 |
1986年 | 6篇 |
1985年 | 16篇 |
1984年 | 7篇 |
1983年 | 8篇 |
1982年 | 9篇 |
1981年 | 7篇 |
1980年 | 5篇 |
1979年 | 15篇 |
1978年 | 5篇 |
1977年 | 3篇 |
1976年 | 6篇 |
1974年 | 5篇 |
1973年 | 8篇 |
1972年 | 9篇 |
1970年 | 3篇 |
1969年 | 2篇 |
1966年 | 3篇 |
排序方式: 共有559条查询结果,搜索用时 781 毫秒
61.
Worthen DB 《Journal of the American Pharmacists Association : JAPhA》2011,51(2):310-312
From the beginning, APhA recognized and embraced the mission of keeping its members and all pharmacists informed and engaged in the progress of the profession. For the entire period encompassed by the Proceedings, print runs were for 1,000 copies or more, a far greater number than the membership list, which allowed for distribution to a broad audience both domestically and internationally. However, it was the meetings in 1856 and 1857 establishing the Report on the Progress of Pharmacy and the publication of NF in 1888 that truly moved the Association to the role of a major provider of information to the pharmacists of America. 相似文献
62.
63.
64.
Common and distinct intracellular signaling pathways in human neutrophils utilized by platelet activating factor and FMLP. 总被引:18,自引:1,他引:18
下载免费PDF全文
![点击此处可从《The Journal of clinical investigation》网站下载免费的PDF全文](/ch/ext_images/free.gif)
J A Nick N J Avdi S K Young C Knall P Gerwins G L Johnson G S Worthen 《The Journal of clinical investigation》1997,99(5):975-986
Stimulation of human neutrophils with chemoattractants FMLP or platelet activating factor (PAF) results in different but overlapping functional responses. We questioned whether these differences might reflect patterns of intracellular signal transduction. Stimulation with either PAF or FMLP resulted in equivalent phosphorylation and activation of the mitogen-activated protein kinase (MAPk) homologue 38-kD murine MAP kinase homologous to HOG-1 (p38) MAPk. Neither FMLP nor PAF activated c-jun NH2-terminal MAPk (JNKs). Under identical conditions, FMLP but not PAF, resulted in significant p42/44 (ERK) MAPk activation. Both FMLP and PAF activated MAP kinase kinase-3 (MKK3), a known activator of p38 MAPk. Both MAP ERK kinase kinase-1 (MEKK1) and Raf are activated strongly by FMLP, but minimally by PAF. Pertussis toxin blocked FMLP-induced activation of the p42/44 (ERK) MAPk cascade, but not that of p38 MAPk. A specific p38 MAPk inhibitor (SK&F 86002) blocked superoxide anion production in response to FMLP and reduced adhesion and chemotaxis in response to PAF or FMLP. These results demonstrate distinct patterns of intracellular signaling for two chemoattractants and suggest that selective activation of intracellular signaling cascades may underlie different patterns of functional responses. 相似文献
65.
66.
Abstract The objective of this study was to evaluate the utility of a polymerase chain reaction (PCR) assay in detecting Mycobacterium tuberculosis in bronchoalveolar lavage (BAL) specimens of patients suspected of having active pulmonary tuberculosis (TB) but who were sputum smear-negative. Patients undergoing investigation for suspected pulmonary TB at the University Hospital, Kuala Lumpur, and who were sputum smear-negative underwent fibreoptic bronchoscopy and BAL. One portion of each lavage specimen was submitted for smear examination for acid-fast bacilli and mycobacterial culture and the other portion assayed by PCR for the presence of a 562-base pair DNA segment belonging to the insertion sequence IS986, unique to the M. tuberculosis complex. As controls, lavage specimens from patients with other lung lesions were also similarly tested. The PCR assay gave a positivity rate of 80.9% (55 of 68) compared with 8.8% of smear examination and 7.4% of culture for detecting M. tuberculosis in BAL specimens. The assay was positive in two of 45 BAL specimens from 35 control subjects. The PCR assay was more sensitive than smear and culture in detecting M. tuberculosis in BAL specimens of patients with sputum smear-negative pulmonary TB. 相似文献
67.
Bernard MY Cheung THY Au SY Chan CM Lam SH Lau RP Lee SF Lee WS Lo EHF Sin MY Tang HH Tsang 《Experimental & Clinical Cardiology》2005,10(1):21-24
BACKGROUND:
Psychosocial stress can be the cause or the consequence of hypertension.OBJECTIVE:
To study the association between hypertension and anxiety or depression in adults from Hong Kong, China.SUBJECTS AND METHODS:
Patients with diagnosed hypertension (n=197) were recruited to complete the Hospital Anxiety and Depression Scale (HADS) questionnaire. The control group comprised 182 normotensive subjects recruited using random telephone numbers.RESULTS:
The score in the anxiety subscale (HADS-A) of the HADS correlated with age (r= −0.23, P<0.001) and sex (r=0.11, P=0.042), and was found to be higher in women. The score in the depression subscale (HADS-D) correlated with age (r=0.17, P=0.003) and hypertension (r=0.12, P=0.039), but not with sex (r=0.02, P=0.68). When the control subjects were matched for sex and age with the subjects with hypertension, the mean HADS-A score was 5.51±0.41 in 113 hypertensive subjects and 4.38±0.39 in 113 normotensive subjects (P=0.047). The mean HADS-D score was 5.56±0.39 in the hypertensive and 4.76±0.32 in the normotensive subjects (P=0.11). Multiple regression analysis using data from both groups indicated that the HADS-A score was related to the HADS-D score (β=0.49, P<0.001), age (β= −0.25, P<0.001) and sex (β=0.12, P=0.01) (R2=0.28), whereas the HADS-D score was related to the HADS-A score (β=0.48, P<0.001), age (β=0.30, P<0.001), positive smoking status (β=0.13, P=0.004) and lack of exercise habit (β=0.12, P=0.008) (R2=0.31). Hypertension was related to waist circumference, history of parental hypertension and age (R2=0.38, P<0.001). Anxiety and depression scores were rejected as independent variables.CONCLUSIONS:
Hypertension was associated with anxiety but not depression; however, age, history of parental hypertension and central obesity appeared to have a stronger association with hypertension in adults from Hong Kong. 相似文献68.
G S Worthen 《The American review of respiratory disease》1987,136(2):455-458
Neutrophil-mediated endothelial injury may occur in the development of an inflammatory site. The factors contributing to the severity of endothelial injury induced by neutrophils are beginning to be recognized. Close and prolonged contact between neutrophils and endothelial cell, induced by a variety of mediators, may allow for secretion of toxic products into a limited area and thus overwhelming local defense mechanisms. Similarly, the intensity of neutrophil secretion (proteases or oxygen radicals) once the neutrophil is adherent appears to be of critical importance. In this regard, the ability of bacterial lipopolysaccharides and lipid mediators such as PAF and LTB4 to "prime" the neutrophil for enhanced secretion of oxygen radicals and proteases may be relevant to endothelial injury especially in the complex environment of an inflammatory focus. 相似文献
69.
Boumsell L; Bernard A; Reinherz EL; Nadler LM; Ritz J; Coppin H; Richard Y; Dubertret L; Valensi F; Degos L; Lemerle J; Flandrin G; Dausset J; Schlossman SF 《Blood》1981,57(3):526-530
Tumor cells from eight adult patients with T-cell chronic malignancies were investigated with a series of monoclonal antibodies recognizing T- cell differentiation antigens. This series allowed definition of discrete subpopulations of mature T cells with functional specialization. All six patients with Sezary syndrome and one patient with T-chronic lymphocytic leukemia had cells with the same phenotype as normal helper/inducer T cells, whereas the other patient with T- chronic lymphocytic leukemia had cell with the same phenotype as normal cytotoxic/suppressor T cells. Some clinical manifestations observed in these patients may reflect retention of functional activities by their malignant cells. 相似文献
70.
Increased expression of major histocompatibility complex antigens on lymphocytes from aged mice 总被引:6,自引:0,他引:6
C L Sidman E A Luther J D Marshall K A Nguyen D C Roopenian S M Worthen 《Proceedings of the National Academy of Sciences of the United States of America》1987,84(21):7624-7628
Many studies have reported age-related changes in immune responses that could be due to alterations in lymphoid cell numbers or functions. Here we report the results of studies using immunofluorescent staining and in vitro assays of cellular function to compare the expression of cell surface antigens on lymphocytes from mice up to 2 years of age. No significant changes were observed in the frequencies of spleen cells bearing class I or class II major histocompatibility complex (MHC) antigens, surface immunoglobulin, or Thy-1, Ly-1, Ly-2, or L3T4 antigens. However, the densities (per cell) of both class I and class II MHC antigens were increased significantly on cells from aged as compared to young mice, whereas the densities of the other cell surface antigens studied were unchanged or slightly decreased. The increased levels of MHC antigen expression in old relative to young mice were shown to be functionally significant regarding immunological stimulation. These data suggest that T-cell clones silent in young individuals may be activated in comparable situations in older animals, leading to immunological alterations perhaps including increased autoreactivity. 相似文献