全文获取类型
收费全文 | 2096篇 |
免费 | 200篇 |
国内免费 | 14篇 |
专业分类
耳鼻咽喉 | 20篇 |
儿科学 | 47篇 |
妇产科学 | 25篇 |
基础医学 | 359篇 |
口腔科学 | 35篇 |
临床医学 | 283篇 |
内科学 | 471篇 |
皮肤病学 | 39篇 |
神经病学 | 241篇 |
特种医学 | 43篇 |
外科学 | 251篇 |
综合类 | 40篇 |
一般理论 | 1篇 |
预防医学 | 182篇 |
眼科学 | 34篇 |
药学 | 156篇 |
中国医学 | 3篇 |
肿瘤学 | 80篇 |
出版年
2022年 | 18篇 |
2021年 | 47篇 |
2020年 | 11篇 |
2019年 | 33篇 |
2018年 | 30篇 |
2017年 | 36篇 |
2016年 | 30篇 |
2015年 | 37篇 |
2014年 | 46篇 |
2013年 | 74篇 |
2012年 | 119篇 |
2011年 | 130篇 |
2010年 | 77篇 |
2009年 | 82篇 |
2008年 | 109篇 |
2007年 | 121篇 |
2006年 | 105篇 |
2005年 | 125篇 |
2004年 | 108篇 |
2003年 | 89篇 |
2002年 | 102篇 |
2001年 | 38篇 |
2000年 | 50篇 |
1999年 | 47篇 |
1998年 | 29篇 |
1997年 | 24篇 |
1996年 | 18篇 |
1995年 | 12篇 |
1994年 | 18篇 |
1993年 | 17篇 |
1992年 | 40篇 |
1991年 | 40篇 |
1990年 | 28篇 |
1989年 | 25篇 |
1988年 | 23篇 |
1987年 | 34篇 |
1986年 | 41篇 |
1985年 | 35篇 |
1984年 | 27篇 |
1983年 | 30篇 |
1980年 | 13篇 |
1979年 | 13篇 |
1978年 | 14篇 |
1977年 | 13篇 |
1976年 | 10篇 |
1975年 | 10篇 |
1972年 | 10篇 |
1971年 | 11篇 |
1969年 | 13篇 |
1968年 | 10篇 |
排序方式: 共有2310条查询结果,搜索用时 0 毫秒
61.
Athena Hadjixenofontos Michael A. Schmidt Patrice L. Whitehead Ioanna Konidari Dale J. Hedges Harry H. Wright Ruth K. Abramson Ramkumar Menon Scott M. Williams Michael L. Cuccaro Jonathan L. Haines John R. Gilbert Margaret A. Pericak‐Vance Eden R. Martin Jacob L. McCauley 《Annals of human genetics》2013,77(1):9-21
Despite the increasing speculation that oxidative stress and abnormal energy metabolism may play a role in Autism Spectrum Disorders (ASD), and the observation that patients with mitochondrial defects have symptoms consistent with ASD, there are no comprehensive published studies examining the role of mitochondrial variation in autism. Therefore, we have sought to comprehensively examine the role of mitochondrial DNA (mtDNA) variation with regard to ASD risk, employing a multi‐phase approach. In phase 1 of our experiment, we examined 132 mtDNA single‐nucleotide polymorphisms (SNPs) genotyped as part of our genome‐wide association studies of ASD. In phase 2 we genotyped the major European mitochondrial haplogroup‐defining variants within an expanded set of autism probands and controls. Finally in phase 3, we resequenced the entire mtDNA in a subset of our Caucasian samples (~400 proband‐father pairs). In each phase we tested whether mitochondrial variation showed evidence of association to ASD. Despite a thorough interrogation of mtDNA variation, we found no evidence to suggest a major role for mtDNA variation in ASD susceptibility. Accordingly, while there may be attractive biological hints suggesting the role of mitochondria in ASD our data indicate that mtDNA variation is not a major contributing factor to the development of ASD. 相似文献
62.
63.
Adeno-associated virus(AAV) is an essential instrument in the neuroscientist's toolkit, which allows delivery of DNA to provide labeling with fluorescent proteins or genetic instructions to regulate gene expression. In the field of neural regeneration, the transduction of neurons enables the observation and regulation of axon growth and regeneration, and in the future will likely be a mechanism for delivering molecular therapies to promote sprouting and regeneration after central nervous system injury. Traditional formulations of AAV preparations permit efficient viral transduction under physiologic conditions, but an improved understanding of the mechanistic limitations of AAV transduction may facilitate production of more resilient AAV strains for investigative and therapeutic purposes. We studied AAV transduction in the context of prior exposure of AAV serotype 8(AAV8) to environmental p H within the range encountered during endosomal endocytosis(p H 7.4 to p H 4.4), during which low p H-triggered structural and autoproteolytic changes to the viral capsid are believed to be necessary for endosome escape and virus uncoating. Due to the fundamental nature of these processes, we hypothesized that premature exposure of AAV8 particles to acidic p H would decrease viral transduction of HT1080 cells in vitro, as measured by fluorescent reporter gene expression using high-content imaging analysis. We found that increasingly acidic incubation conditions were associated with concomitant reductions in transduction efficiency, and that quantitative levels of reporter gene expression in transduced cells were similarly decreased. The biggest decrease in transduction occurred between p H 7.4 and p H 6.4, suggesting the possible co-occurrence of a p H-associated event and viral inactivation within that range. Taken together, these findings indicate that exposure of AAV8 to acidic p H for as little as 1 hour is deleterious to transduction ability. Future studies are necessary to understand the p H-associated causative mechanisms involved. This study was approved by the University of Miami Institutional Animal Care and Use Committee, USA(Protocol #18-108-LF) on July 12, 2018. 相似文献
64.
Sandra Rincon Lina P. Carvajal Sara I. Gomez-Villegas Aura M. Echeverri Rafael Rios An Dinh Claudia Pedroza Karen M. Ordoez Esteban Nannini Zhizeng Sun Vance G. Fowler Barbara E. Murray William R. Miller Timothy Palzkill Lorena Diaz Cesar A. Arias Jinnethe Reyes 《Journal of clinical microbiology》2021,59(4)
65.
66.
Melmed S Vance ML Barkan AL Bengtsson BA Kleinberg D Klibanski A Trainer PJ 《Pituitary》2002,5(3):185-196
Growth-hormone (GH) secreting adenomas, including acromegaly, account for approximately one-sixth of all pituitary adenomas and are associated with mortality rates at least twice that of the general population. The ultimate goal of therapy for acromegaly is normalization of morbidity and mortality rates achieved through removal or reduction of the tumor mass and normalization of insulin-like growth factor I (IGF-I) levels. Previously published efficacy results of current treatment modalities (surgery, conventional radiation, and medical therapy with dopamine agonists and somatostatin analogs) are often difficult to compare because of the different criteria used to define cure (some of which are now considered inadequate). For each of these modalities, pooled data from a series of acromegaly studies were reviewed for rates of IGF-I normalization, a currently accepted definition of cure. The results showed overall cure rates of approximately 10% for bromocriptine, 34% for cabergoline, 36% for conventional radiation, 50–90% for surgery for microadenomas and less than 50% for macroadenomas, and 54–66% for octreotide. These cure rates based on IGF-I normalization are generally less than those reported for cure based solely on GH levels. Novel new therapies for acromegaly include the somatostatin analog, lanreotide, Gamma Knife radiosurgery, and pegvisomant, the first in its class of new GH receptor antagonists. Although it does not appear that Gamma Knife radiosurgery results in significantly higher cure rates or fewer complications, it does provide a notable improvement in delivery compared with conventional radiation. Early studies have reported IGF-I normalization in 48% of lanreotide-treated patients and up to 97% of pegvisomant-treated. 相似文献
67.
68.
69.
Extensive non-small cell lung cancer treated with mitomycin, cisplatin, and vindesine (MiPE): a Southwest Oncology Group Study 总被引:1,自引:0,他引:1
Ninety-seven previously untreated patients with metastatic non-small cell lung cancer were treated with combination chemotherapy consisting of mitomycin (10 mg/m2) on Day 1, cisplatin (50 mg/m2) on Days 1 and 22, and vindesine (3 mg/m2) on Days 1 and 22 (MiPE). MiPE was repeated at 6-week intervals until disease progression or unacceptable toxicity. The overall response rate was 33%. There were seven complete responses (7%) and 25 partial responses (26%). The median progression-free interval for responding patients was 7 months. Median survival for all patients was 5 months, with 16% surviving 1 year. One patient died from sepsis while neutropenic. The results with MiPE treatment for patients with non-small cell lung cancer compare favorably to other mitomycin-vinca combinations previously tested in the Southwest Oncology Group. 相似文献
70.
Andrew Wilhelm Manigault Ian Michael Handley Summer Rain Whillock 《Journal of medical Internet research》2015,17(2)