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排序方式: 共有359条查询结果,搜索用时 31 毫秒
61.
Arisawa T Tahara T Shibata T Nagasaka M Nakamura M Kamiya Y Fujita H Nakamura M Yoshioka D Arima Y Okubo M Hirata I Nakano H 《Journal of clinical immunology》2008,28(1):44-49
We investigated the association between ulcerative colitis (UC) and polymorphisms of IL-17A (rs2275913, G-197A) and IL-17F
(rs763780, 7488T/C) genes. We employed the multiplex PCR-SSCP method to detect gene polymorphisms. Both the numbers of -197A
(IL-17A) and 7488T (IL-17F) alleles were significantly correlated to the development of UC. The frequencies of -197A/A and
7488T/T genotypes in the UC group were significantly higher than those in the non-UC group. An adjusted analysis revealed
that -197A and 7488T alleles were independent risk factors for the developing UC. In addition, both polymorphisms were significantly
associated with the pancolitis phenotype. Furthermore, -197A allele was significantly correlated to the chronic relapsing
phenotype and -197A/A homozygote was more frequent in steroid-dependent cases, whereas 7488T allele was correlated with the
chronic continuous phenotype. Our results provided the first evidence that -197A (IL-17A) and 7488T (IL-17F) alleles may influence
the susceptibility to and pathophysiological features of UC independently. 相似文献
62.
Katsunobu?Taki Daisuke?Hashimoto Shigeki?Nakagawa Nobuyuki?Ozaki Shinjiro?Tomiyasu Masaki?Ohmuraya Kota?Arima Takayoshi?Kaida Takaaki?Higashi Keita?Sakamoto Kazuya?Sakata Hirohisa?Okabe Hidetoshi?Nitta Hiromitsu?Hayashi Akira?Chikamoto Toru?Beppu Hiroshi?Takamori Masahiko?Hirota Hideo?BabaEmail author 《Surgery today》2017,47(9):1104-1110
Purpose
Pancreatic neuroendocrine tumor (PNET) is relatively rare and has a generally better prognosis than does pancreatic cancer. However, as its prognosis in patients with lymph node metastasis (LNM) is unclear, lymph node dissection for PNET is controversial. Our study aimed to clarify the significance of LNM in PNET.Methods
We retrospectively examined 83 PNET patients who underwent pancreatic resections with lymph node dissection at Kumamoto University Hospital, Saiseikai Kumamoto Hospital, and Kumamoto Regional Medical Center from April 2001 to December 2014. Their clinicopathological parameters were analyzed by the absence or presence of LNM, and with regard to the disease-free survival (DFS) and overall survival (OS). A predictive score of LNM was also made using the age, tumor size, primary tumor location, and tumor function.Results
Although the 5-year OS was 74.8% for LNM+ and 94.6% for LNM? (P?=?0.002), LNM was not an independent risk factor for the OS in a multivariate analysis. However, tumors larger than 1.8 cm were found to be an independent prognostic factor, and the cut-off value for the predictive score was 1.69.Conclusions
Although LNM was not an independent prognostic factor, lymph node dissection is recommended for patients whose predictive score is larger than 1.69.63.
P L Di Patre S L Read J L Cummings U Tomiyasu L M Vartavarian D L Secor H V Vinters 《Archives of neurology》1999,56(10):1254-1261
OBJECTIVES: To quantify the progression of senile plaques, neurofibrillary tangles, cerebral amyloid angiopathy, and microglial activation in the cortex and white matter of patients with Alzheimer disease evaluated at both biopsy and subsequent autopsy and correlate these changes with the progression of neurologic impairment. SETTING: Academic referral center for patient with Alzheimer disease. PATIENTS: Four patients meeting the clinical criteria for Alzheimer disease, enrolled in a pilot study for the evaluation of response to intracerebroventricular administration of bethanechol chloride. The patients were followed up until death occurred and autopsy was performed. RESULTS: All 4 patients had progressive deterioration from the time of biopsy to autopsy (9-11 years). Pathological investigations showed a striking increase in the density of senile plaques and neurofibrillary tangles in 2 of 4 patients from biopsy to autopsy, and a significant increase in microglial activation in 1 of 4 cases. Severity of cerebral amyloid angiopathy varied significantly among patients, 1 of whom displayed striking amyloid deposition with associated subcortical white matter atrophy. CONCLUSIONS: These unique data demonstrate that the progressive neurologic impairment in Alzheimer disease is accompanied by a significant increase in senile plaque and neurofibrillary tangle counts in the frontal cortex and, possibly in some patients, by increased microglial cell activation. Cerebral amyloid angiopathy was associated with significant white matter disease. 相似文献
64.
Berge A. Minassian Jesus Sainz Jose M. Serratosa Manyee Gee Lise M. Sakamoto Saeed Bohlega Guy Geoffroy Cathy Barr Steve W. Scherer Uwamie Tomiyasu Stirling Carpenter Karen Wigg A. V. Sanghvi Antonio V. Delgado-Escueta 《Annals of neurology》1999,45(2):262-265
In 1995, we mapped a gene for Lafora's progressive myoclonus epilepsy in chromosome 6q23-25. In 1997 and 1998, we reduced the size of the locus to 300 kb, and an international collaboration identified mutations in the protein tyrosine phosphatase gene. Here, we examine for heterogeneity through the admixture test in 22 families and estimate the proportion of linked families to be 75 to 85%. Extremely low posterior probabilities of linkage (Wi), exclusionary LOD scores, and haplotypes identify 4 families unlikely to be linked to chromosome 6q24. Ann Neurol 1999;45:262–265 相似文献
65.
66.
Akiyuki Tomiyasu Masayuki Nakamura Mio Ichiba Shuichi Ueno Shinji Saiki Mizuki Morimoto Jan Kobal Yasufumi Kageyama Toshio Inui Koichi Wakabayashi Tatsuo Yamada Yuji Kanemori Hans H. Jung Haruhiko Tanaka Satoshi Orimo Zaid Afawi Ilan Blatt Jan Aasly Hiroshi Ujike Dusica Babovic‐Vuksanovic Keith A. Josephs Rie Tohge Guilherme Riccioppo Rodrigues Nicolas Dupré Hidetaka Yamada Fusako Yokochi Katya Kotschet Takanobu Takei Monika Rudzińska Andrzej Szczudlik Silvana Penco Masaki Fujiwara Kana Tojo Akira Sano 《American journal of medical genetics. Part B, Neuropsychiatric genetics》2011,156(5):620-631
Chorea‐acanthocytosis (ChAc) is a rare autosomal recessive neurodegenerative disorder caused by loss of function mutations in the vacuolar protein sorting 13 homolog A (VPS13A) gene that encodes chorein. It is characterized by adult‐onset chorea, peripheral acanthocytes, and neuropsychiatric symptoms. In the present study, we performed a comprehensive mutation screen, including sequencing and copy number variation (CNV) analysis, of the VPS13A gene in ChAc patients. All 73 exons and flanking regions of VPS13A were sequenced in 35 patients diagnosed with ChAc. To detect CNVs, we also performed real‐time quantitative PCR and long‐range PCR analyses for the VPS13A gene on patients in whom only a single heterozygous mutation was detected. We identified 36 pathogenic mutations, 20 of which were previously unreported, including two novel CNVs. In addition, we investigated the expression of chorein in 16 patients by Western blotting of erythrocyte ghosts. This demonstrated the complete absence of chorein in patients with pathogenic mutations. This comprehensive screen provides an accurate and useful method for the molecular diagnosis of ChAc. © 2011 Wiley‐Liss, Inc. 相似文献
67.
Okubo M Tahara T Shibata T Nakamura M Yoshioka D Maeda Y Yonemura J Ishizuka T Arisawa T Hirata I 《Journal of gastroenterology》2011,46(2):175-182
Background
Magnifying narrow-band imaging (NBI) endoscopy visualizes superficial gastric mucosal and capillary patterns. We aimed to investigate changes in gastric mucosal patterns seen by magnifying NBI endoscopy after Helicobacter pylori eradication. 相似文献68.
Functional promoter polymorphisms of macrophage migration inhibitory factor in peptic ulcer diseases
Shiroeda H Tahara T Shibata T Nakamura M Yamada H Nomura T Hayashi R Saito T Fukuyama T Otsuka T Yano H Ozaki K Tsuchishima M Tsutsumi M Arisawa T 《International journal of molecular medicine》2010,26(5):707-711
Macrophage migration inhibitory factor (MIF) is a key proinflammatory mediator, which plays a pivotal role in inflammatory and immune diseases. We attempted to clarify the association of functional polymorphisms of MIF gene promoter with the development of gastro-duodenal ulcer. The study was performed in 471 stocked DNAs obtained from the subjects, including 93 healthy volunteers, with no evidence of gastric malignancy. We employed the PCR-SSCP method to detect gene polymorphisms. In all 471 DNAs, 92 and 43 were obtained from gastric and duodenal ulcer patients, respectively. By an unadjusted analysis, infection with Helicobacter pylori (H. pylori), male gender and non-steroidal anti-inflammatory drug (NSAID/aspirin) use were significantly associated with a risk for developing a gastric ulcer, whereas MIF promoter polymorphisms were not. On the other hand, infection with H. pylori, male gender and 7-CATT repeat at position -794 were significantly associated with the development of a duodenal ulcer, whereas NSAID/ aspirin use was not. By the analysis after adjustment for age, gender, NSAID/aspirin use and H. pylori infection status, 7/7-CATT homozygote had a significantly increased risk for the development of duodenal ulcers (OR, 6.31; 95% CI, 1.50-26.6; p=0.012). No factors were significantly associated with the development of peptic ulcers in NSAID/aspirin users. Our results suggested that tetranucleotide repeat polymorphism of MIF gene promoter might be associated with the development of duodenal ulcers. 相似文献
69.
Anna Nakamura Takato Ueno Yumihiko Yagi Koji Okuda Toshiro Ogata Toru Nakamura Takuji Torimura Hideki Iwamoto Sivakumar Ramadoss Michio Sata Victor Tsutsumi Kaori Yasuda Yumi Tomiyasu Kenichi Obayashi Kosuke Tashiro Satoru Kuhara 《Medical molecular morphology》2010,43(2):107-115
We compared the morphological and functional characteristics of cultured unfrozen hepatic stellate cells (HSCs) and cryopreserved HSCs obtained from human livers. We used liver tissues obtained by surgical resection from patients with metastatic liver cancer or with hepatocellular carcinoma. HSCs were isolated and allowed to spread in culture. Comparison of morphological and functional features between the unfrozen HSCs and cryopreserved HSCs was performed at each passage using the following techniques: light microscopy, immunohistochemistry, cell growth curve, metallothionein (MTT) assay, and PI staining, Western blot, real-time polymerase chain reaction (PCR), and gene expression analysis using microarrays. The purity of HSCs was more than 90% in all passages. α-Smooth muscle actin (SMA-)positive HSCs gradually increased in successive passages, and the positive cell rate and rate of increase in cell number were similar in both groups. Expression of platelet-derived growth factor (PDGF) receptor, transforming growth factor (TGF)-β receptor, and α-SMA mRNAs and protein was similar during each passage in the two groups. Gene expression was nearly identical at each passage in unfrozen and frozen/thawed samples obtained from the same patient. In conclusion, an adequate protocol for the cryopreservation of human primary cultured HSCs could be established. 相似文献
70.
Nobuyuki Toshikuni Mikihiro Tsutsumi Tomiyasu Arisawa 《World journal of gastroenterology : WJG》2014,20(26):8393-8406
Alcoholic liver disease (ALD) and nonalcoholic fatty liver disease (NAFLD) are serious health problems worldwide. These two diseases have similar pathological spectra, ranging from simple hepatic steatosis to steatohepatitis, liver cirrhosis, and hepatocellular carcinoma. Although most subjects with excessive alcohol or food intake experience simple hepatic steatosis, a small percentage of individuals will develop progressive liver disease. Notably, both ALD and NAFLD are frequently accompanied by extrahepatic complications, including cardiovascular disease and malignancy. The survival of patients with ALD and NAFLD depends on various disease-associated conditions. This review delineates the clinical characteristics and outcomes of patients with ALD and NAFLD by comparing their epidemiology, the factors associated with disease susceptibility and progression, and the predictors and characteristics of outcomes. A comprehensive understanding of the characteristics and outcomes of ALD and NAFLD is imperative in the management of these chronic liver diseases. 相似文献