营养素摄入水平与前列腺癌发病因素的相关分析

目的:探讨营养素摄入水平与前列腺癌发病危险的关系。方法:对经病理组织学确诊的96例前列腺癌新发病例实施1∶3配比的病例-对照研究。三类对照共计288例(其中医院癌对照96例,医院非癌对照96例,正常对照96例)。配比条件为同性别、同民族和年龄相差≤±5岁。结果:经条件Logistic回归分析结果显示,总热量(OR值1.22~2.06,P值分别为0.78、0.04和0.032)、总脂肪(OR值1.06~1.89,P值分别为0.98、0.021和0.019)、饱和脂肪酸(OR值1.08~1.79,P值分别为0.92、0.023和0.032)、不饱和脂肪酸(OR值1.07~2.81,P值分别为0.88、0.027和0.007 1)以及动物脂肪(OR值1.14~2.56,P值分别为0.87、0.047和0.008)与前列腺癌发病危险呈正相关;硒与前列腺癌发病危险呈负相关(OR值0.57~0.84,P值分别为0.84、0.031和0.030)。总热量、总脂肪、饱和脂肪酸、动物脂肪与前列腺癌发病存在一定的剂量反应关系。结论:营养素摄入过程中的高脂和微量元素硒缺乏与前列腺癌发病危险存在关联。     

Prognostic and incremental value of computed tomography-based radiomics from tumor and nodal regions in esophageal squamous cell carcinoma

Objective: This study aimed to evaluate the prognostic value of preoperative radiomics and establish an integrated model for esophageal squamous cell cancer(ESCC).Methods: A total of 931 patients were retrospectively enrolled in this study(training cohort, n=624; validation cohort, n=307). Radiomics features were obtained by contrast-enhanced computed tomography(CT) before esophagectomy. A radiomics index was set based on features of tumor and reginal lymph nodes by using the least absolute shri...     

Sleep‐disordered breathing in heart failure patients with different etiologies

BackgroundThe prevalence of sleep‐disordered breathing (SDB) is closely related to the severity of heart failure (HF), and the severity of HF is different in patients with HF of different etiologies. Hypothesis: This study aimed to explore the prevalence of SDB in patients with HFof different etiologies.MethodsHospitalized HF patients were consecutively enrolled. All patients underwent portable overnight cardiorespiratory polygraphy. Patients were divided into five groups according to the etiology of HF: ischemic, hypertensive, myocardial, valvular, and arrhythmic. The prevalence of SDB and clinical data was compared among the five groups.ResultsIn total, 248 patients were enrolled in this study. The prevalence of SDB in HF was 70.6%, with the prevalence of obstructive sleep apnea (OSA) at 47.6% and central sleep apnea (CSA) at 23.0%. Patients were divided into five groups: ischemic, hypertensive, myocardial, valvular, and arrhythmic. The prevalence of SDB among the five groups was 75.3%, 81.4%, 77.8%, 51.9%, and 58.5% (p = .014), respectively. The prevalence of OSA among the five groups was 42.7%, 72.1%, 36.1%, 37.0%, and 49.1% (p = .009), whereas the CSA was 32.6%, 9.3%, 41.7%, 14.8%, and 9.4% (p < .001), respectively.ConclusionsSDB is common in HF patients. The prevalence and types of SDB varied in HF with different etiologies, which may be related to the different severities of HF. SDB was highly prevalent in patients with ischemic, hypertensive, and myocardial HF. Hypertensive HF patients were mainly complicated with OSA, while myocardial HF patients were mainly complicated with CSA. Both conditions were highly prevalent in ischemic HF patients. The prevalence of SDB was relatively low in valvular and arrhythmic HF patients, and OSA was the main type.     

Development and Primary Application of an Indirect ELISA Based on Rep Protein to Analyze Antibodies against Porcine Cocirvirus-like Virus (PCLV)

Porcine circovirus-like virus (PCLV) is a member of circovirus that contains a single-strand DNA genome, which may be one of the pathogens that causes diarrheal symptoms in pigs. The Rep protein encoded by the genome of PCLV may be responsible for viral genome replication. The development of serological detection methods for PCLV is of great necessity for clinical diagnosis, as well as epidemiological investigations. Therefore, this study attempted to build an indirect enzyme-linked immunosorbent assay (ELISA) to examine antibodies against PCLV based on the His-tagged recombinant Rep protein. Full-length PCLV Rep protein was induced and expressed in E. coli and was purified as an antigen to establish an ELISA detection kit. The purified Rep protein was used to inject into mice to produce specific antibodies. There was no cross-reaction of Rep-based ELISA with antisera against other porcine viruses. The intra-assay and inter-assay coefficient variations (CVs) were 0.644–8.211% and 0.859–7.246%, respectively, indicating good repeatability. The non-cross-reaction with TGEV, PRRSV and PCV2 testing showed high sensitivity and high specificity for this ELISA assay. A total of 1593 serum samples collected from different pig farms in Jiangxi Province were tested for anti-PCLV Rep antibodies, and 284 (17.83%) of the 1593 samples were Rep antibody positive. Altogether, the indirect ELISA detection tool developed in this study could be applied to examine serum of PCLV antibodies with good repeatability, high sensitivity and high specificity. In addition, field sample detection results suggested that the PCLV antibody has a low prevalence in pig populations in Jiangxi Province of China.     

优化美罗培南治疗老年脓毒血症的临床分析

目的 分析血药浓度监测(TDM)优化美罗培南治疗老年脓毒血症的临床疗效。方法 选择2021年1月至2022年2月我院收治的脓毒血症老年患者64例,随机分为对照组31例,观察组33例。对照组接受常规美罗培南治疗,观察组接受TDM优化美罗培南,对比治疗1周后两组病情变化、生化指标、肺功能、实验室指标与不良反应等。结果 治疗1周后,观察组APACHEⅡ评分/SOFA评分低于对照组(P<0.05),观察组肌酐、胆红素、AST、ALT、EVLW、PVPI下降(P<0.05),观察组白细胞、降钙素原、C-反应蛋白水平低于对照组(P<0.05),观察组美罗培南药物谷浓度与峰浓度高于对照组(P<0.05),观察组PK/PD参数最低抑菌浓度(MIC)为1、2、4时血清药物浓度高于MIC的时间(%T>MIC)优于对照组(P<0.05)。结论 65岁以上老年脓毒血症患者,实施TDM优化美罗培南治疗,可降低病情严重水平,调控机体生化指标、炎症指标,提高药物抗菌活性、药物有效浓度,改善患者肺功能,减少药物不良反应,具有临床意义。     

硫酸长春新碱传递体的制备及其离体透皮研究

目的:筛选制备硫酸长春新碱传递体(VCR-T)的最佳工艺,预测其作为VCR新制剂的可行性。方法:采用干膜超声法通过正交试验优化制备工艺;激光散射粒径分析仪测量粒径及其分布,透射电镜观察形态,HPLC测定包封率;改良的Franz扩散池进行体外透皮试验。结果:最优处方及工艺:卵磷脂:去氧胆酸钠为70:20,载体:VCR为45:10;pH7.3,水合30min;制备的硫酸长春新碱传递体为淡黄色透明胶体溶液,平均粒径94nm,外形圆整光滑,分布均匀,包封率为90%;以零级速率透过皮肤,24h累积透皮吸收率为63.8%。结论:VCR-T有望成为VCR临床给药的一种新给药系统。     

尼卡地平静脉点滴用于妊娠期高血压急症紧急降压的疗效观察

目的 观察静脉用尼卡地平治疗孕期高血压急症的降压效果及对母婴的安全性。方法 观察16例妊娠期高血压急症病人接受静点尼卡地平治疗,心电监护仪观察24h动态血压、心率并评价其稳定性,电子监护仪监测胎心率用药前后变化,B超测定脐动脉血流S/D值并对新生儿进行Apgar评分、出生体重及新生儿预后的分析,了解其安全性。结果 全部患者静点尼卡地平后5～40min内血压可降至预期血压(140～150/90～100)mmHg,并能保持血压平稳。血压下降初始可伴心率加快,但多无症状,2h内即恢复。治疗前后胎心监护、胎儿脐血流无明显改变。结论 尼卡地平用于重度妊高症降压治疗是有效的,可平稳维持血压,未发现有严重不良反应,对胎儿安全性与其他钙拮抗剂相似。     

Exploring the mechanism of Buyang Huanwu decoction in the treatment of lumbar disc herniation based on network pharmacology and molecular docking

Buyang Huanwu decoction (BYHWD), as one of the traditional Chinese medicine formulas, is widely used in the clinical treatment of lumbar disc herniation (LDH) with curative effect. It has the characteristics of multi-component, multi-target, and mutual synergy, but the mechanism of action is often unclear. It needs some research to explore the molecular mechanism of BYHWD in the treatment of LDH based on network pharmacology and molecular docking. Screen the active compounds of BYHWD and predict drug-related gene/protein targets, which could determine the specific target of BYHWD in the treatment of LDH. Construct the “Drugs-Compounds-Targets” network and search for the core targets. Use Gene Ontology functional enrichment analysis, Kyoto Encyclopedia of Genes and Genomes pathway enrichment analysis, and molecular docking verification to explore the possible molecular mechanism. Eighty-two effective compounds and 666 targets of BYHWD, 187 targets for LDH treatment, and 20 core candidate targets were excavated. A total of 3414 entries were identified by Gene Ontology enrichment analysis, 173 related signal pathways were identified by Kyoto Encyclopedia of Genes and Genomes enrichment analysis, and 5 core compounds were identified by molecular docking, which had a good affinity with core genes STAT3, JUN, AKT1, MAPK1, RELA, and PIK3CA. BYHWD may play the role of analgesic and improving function by synergistic anti-inflammatory and analgesic compounds, regulating cell metabolic differentiation, regulating immunity, and anticoagulation. BYHWD in the treatment of LDH may play a role in analgesia and improve function through multiple signaling pathways, including PI3K-Akt, mitogen-activated protein kinase, tumor necrosis factor, and interleukin-17. The PI3K-Akt signaling may be one of the key mechanisms.     

健择加顺铂联合化疗治疗晚期鼻咽癌的疗效观察

目的:观察健择加顺铂治疗晚期鼻咽癌的疗效和毒副作用。方法:吉西他滨1000 mg/m2d1、d8 生理盐水100 ml静脉点滴30 min完成;DDP 100 mg 生理盐水500 ml静脉滴注,d1,或DDP 30 mg 生理盐水250 ml静脉滴注,d1~d3,方案每3周为一周期。结果:17例患者完全缓解(CR)2例,部分缓解(PR)11例,总有效率(CR PR)达76.5%。主要毒副反应为骨髓抑制(对血小板抑制尤为明显)、消化道反应、轻度肝肾功能损及口腔炎等。结论:健择联合顺铂方案治疗复发或转移性鼻咽癌有效率高和耐受性良好,值得临床进一步研究。