鄱阳湖区实施"麋鹿回家计划"对血吸虫病传播影响的调查

目的　了解放归鄱阳湖区的麋鹿血吸虫感染情况,评价“麋鹿放归野外自然繁衍生长计划”（“麋鹿回家计划”）对鄱阳湖区血吸虫病传播的影响。方法　采用GPS项圈定位、人工跟踪观察等方法,于2018年4月-2019年12月调查放归鄱阳湖区麋鹿的种群分布、栖息活动和自然增殖等情况,采用塑料杯顶管孵化法检测麋鹿栖息地动物野粪血吸虫感染情况,采取系统抽样法对麋鹿栖息地草洲进行钉螺分布调查。结果　2018年,共有51头麋鹿放归鄱阳湖区,形成了银宝湖、龙口、南池湖、莲子湖和珠湖等5个麋鹿栖息地,栖息环境主要为鄱阳湖区草洲、圩内农田和山丘林地等。对银宝湖麋鹿栖息地调查显示,至2019年该地麋鹿自然增殖率为25.0%,平均钉螺密度为0.009 ~ 0.039只/0.1 m2,未发现血吸虫感染性钉螺;麋鹿和牛粪平均密度分别为4.6份/hm2和2.1份/hm2,血吸虫阳性率分别为4.35%和13.16%（P ＝ 0.236）,感染强度分别为“+++”和“+”。结论　放归鄱阳湖区的麋鹿可感染血吸虫,并可通过粪便对草洲污染而引起血吸虫病传播扩散。应积极探索麋鹿放归湖区后对当地血吸虫病传播的影响和应对策略,确保鄱阳湖区消除血吸虫病目标的实现和“麋鹿回家计划”的顺利实施。     

敲减OGT对肝细胞脂肪合成的作用研究

目的探讨敲减N-乙酰氨基葡萄糖转移酶(OGT)对肝细胞脂肪合成的影响。方法建立L02正常肝细胞脂肪变模型,蛋白免疫印迹法(Western blot)检测OGT及O-连接糖基化(O-GlcNAc)表达。建立L02细胞OGT敲减细胞系,油酸诱导后检测其脂质形成能力。实时荧光定量PCR(qRT-PCR)和Western blot检测脂肪合成相关酶的mRNA和蛋白表达。采用独立样本t检验。结果Western blot显示L02细胞脂肪变后OGT及O-GlcNAc表达均升高(P<0.05)。shOGT慢病毒感染L02细胞后,OGT mRNA相对表达水平明显下调(P<0.01)。油红O染色显示,L02 shOGT细胞内脂质减少,qRT-PCR显示系列脂肪合成酶:乙酰辅酶A羟化酶、脂肪酸合成酶和硬脂酰辅酶A去饱和酶mRNA相对表达水平均降低,差异具统计学意义(P值均<0.05),蛋白水平与mRNA相对表达量一致。结论敲减OGT后可通过降低O-GlcNAc水平抑制肝细胞脂肪合成。     

射波刀联合TACE治疗原发性肝癌108例

目的:观察射波刀联合经肝动脉化疗栓塞术(transarterial chemoembolization,TACE)治疗原发性肝癌的近期临床疗效和不良反应.方法:回顾性分析2012-01/2013-05采用TACE及射波刀联合治疗的108例肝癌患者的临床资料,并对其疗效和不良反应进行分析.结果:纳入本组分析的108例患者中男性96例,女性12例,男女比例8∶1;患者年龄32-85岁(平均年龄52.6岁).所有患者在射波刀治疗前接受过1-11次TACE治疗(平均2.3次),末次TACE与射波刀治疗间隔14-84 d(平均32 d);射波刀治疗剂量:DT 35-60 Gy/3-6 Fraction3-7 d;随访时间3-19 mo(平均12.8 mo).随访期内,完全缓解(complete response,CR)20例、部分缓解(partial response,PR)70例、病灶稳定(stable disease,SD)18例,治疗近期有效率(CR+PR)83.3%,临床受益率(CR+PR+SD)100%;108例患者中65例射波刀治疗前血清AFP>400μg/L的病例治疗后1-12 wk AFP值下降>50%者42例,其中降至正常范围者28例,治疗过程中及治疗后无3级以上不良反应.结论:射波刀联合TACE术治疗原发性肝癌近期疗效明确,且不良反应较轻,是肝癌综合治疗的安全、有效模式,其长期疗效及副反应需要进一步研究.     

Risk factors for sleep quality disturbances in patients with lumbar spinal stenosis before operation

Sleep and Breathing - We aimed to explore the risk factors of preoperative sleep quality in patients with lumbar spinal stenosis (LSS) and the association of sleep-related beliefs with sleep...     

Analysis of the myoelectric characteristics of genioglossus in REM sleep and its improvement by CPAP treatment in OSA patients

Sleep and Breathing - To reveal the characteristics of genioglossus (GG) activation in moderate and severe obstructive sleep apnea (OSA) patients during rapid eye movement (REM) sleep compared with...     

Elevated levels of T-cell immune response cDNA 7 in patients with immune thrombocytopenia

Objectives

This study aimed to investigate the expression levels of T-cell immune response cDNA 7 (TIRC7) in immune thrombocytopenia (ITP) patients before and after high-dose dexamethasone (HD-DXM) treatment.

Methods

Forty-four patients with ITP were enrolled and received dexamethasone (40 mg/day) for 4 consecutive days. Patients who had platelet counts more than 50 × 10⁹/l or less were defined as responders or non-responders, respectively. Quantitative polymerase chain reaction and enzyme-linked immunosorbent assay were used to measure RNA level and plasma level of TIRC7, respectively.

Results

TIRC7 levels (RNA and plasma level) were significantly higher in ITP than that in control (P < 0.0001). However, after treatment, TIRC7 levels were significantly decreased in responders (P < 0.0001) but not in non-responders (P > 0.05).

Discussions

TIRC7 might be associated with the pathogenesis of ITP, and TIRC7 levels could be used as an indicator to evaluate patients’ response to HD-DXM treatment.     

Noninvasive central pulse pressure is an independent determinant of renal function

The purpose of this study was to investigate the prognostic properties of different BP measurements for renal function decrement and early chronic kidney disease (CKD) in community‐dwelling populations with normal renal function at baseline. A total of 1426 participants were included and followed for a median of 4.8 years (interquartile range, 4.5‐5.2), and central hemodynamic profile and estimated glomerular filtration rate (eGFR) were evaluated. One main outcome was the rapid eGFR decline defined as a decline in eGFR of greater than 3 mL/min per 1.73 m² per year; the other was the new incidence of CKD. At the end of follow‐up, mean eGFR decreased from 93.39 ± 13.46 mL/min per 1.73 m² to 85.72 ± 14.81 mL/min per 1.73 m², and the incidence of rapid eGFR decline and CKD were 20.7% and 5.6%, respectively. In multivariate linear regression analysis, central pulse pressure (PP), age, fasting blood glucose, and concentration of homocysteine were independent determinants of the change in renal function. Not only in the prediction of rapid eGFR decline but also in the incident of CKD, baseline central PP was the only BP component that consistently independently associated with both outcomes after adjustment for various confounders. When compared with subjects in the lowest quartile of central PP, those in the highest quartile demonstrated a significantly increased risk of CKD (hazard ratio [HR], 1.57; 95% confidence interval [CI], 1.08‐2.96; P = .006). The study showed that central PP emerged as an independent predictor of the decline in renal function.