白及不同提取部位对家兔血小板聚集的影响

目的探讨白及不同提取部位对家兔血小板聚集的影响.方法比浊法测定血小板聚集率.结果白及正丁醇提取部位和水溶性部位可显著升高腺苷二磷酸（ADP）诱导的血小板最大聚集率,而乙酸乙酯提取部位可显著抑制ADP诱导的血小板聚集,石油醚提取部位对ADP诱导的血小板聚集无显著影响.各提取部位对血小板数无显著影响.结论白及正丁醇提取部位和水溶性部位是白及止血作用的主要有效部位,其止血作用与其促进血小板聚集作用有关.     

Deep veins of the calf: assessment with color Doppler flow imaging

The authors studied the ability of real-time ultrasound aided by color Doppler flow imaging to demonstrate the deep veins of the calf in ten healthy subjects and 49 patients (91 limbs) with suspected deep venous thrombosis. Posterior tibial and peroneal veins demonstrated flow accentuation in 98% (74 of 75) and 96% (72 of 75), respectively, of the limbs without thrombosis and in all 20 normal limbs. Sixteen legs were involved with deep venous thrombosis. Peroneal veins could not be seen in three legs with marked calf swelling due to proximal thrombosis. There were two cases of thrombosis limited to the calf, nine of popliteal thrombosis (seven with contiguous spread of thrombus), and five of thrombosis limited to above the knee (four with sparing of the deep veins of the calf). Visualization of anterior tibial veins, achieved in 65% of all legs, did not correlate with thrombosis. The authors conclude that color Doppler flow imaging can demonstrate patency of posterior tibial and peroneal veins in most patients without deep venous thrombosis and aid in detection of below-the-knee thrombosis.     

反转录病毒载体介导RNA干扰抑制EphA2在Hela细胞中的蛋白表达

目的：课题前期发现EphA2和配体EphrinAl在宫颈鳞癌细胞和肿瘤相关血管内皮细胞均呈高表达，为基因治疗宫颈癌提供了理想的靶向。以此为基础，利用反转录病毒载体介导的RNA干扰抑制EphA2在Hela宫颈癌细胞中的蛋白表达。方法：实验于2006—01／06在河南省肿瘤病理重点实验室完成。①实验材料：PA317细胞、Hela229细胞购自上海生化细胞所，小鼠NIH3T3细胞为本室保存。EphA2兔抗人多克隆抗体，Actin兔抗人多克隆抗体（美国SantaCruz公司）。②实验方法：从EphA2的mRNA全序列中筛选出1个19nt的靶序列，设计双链发夹结构。以siRNA互补双链寡核苷酸，构建反转录病毒表达载体pSIREN—EphA2，提取质粒，用BglⅡ和EcoRⅠ双酶切进行鉴定。选择经酶切鉴定的正确克隆测序。挑选pSIREN．EphA2抗性克隆细胞扩增，检测转染后Hela细胞中EphA2蛋白的表达水平。结果：@pSIREN-EphA2重组质粒的酶切鉴定：双酶切后形成328bp和6182bp两条电泳条带。②pSIREN-EphA2重组质粒测序：结果与已知序列完全一致。（DEphA2蛋白的表达：经Westernblot分析，重组pSIREN—EphA2转染的Hela细胞中EphA2蛋白表达水平明显降低。结论：通过反转录病毒载体介导的RNA干扰，能够抑制EphA2在Hela细胞中的蛋白表达，为以EphA2为靶点的宫颈癌基因治疗提供新的思路和手段。     

中药缓控释制剂的研究现状及研发思路

中药缓控释制剂研究起步较晚,发展也较慢,处在初级阶段,但近年来,中药复方缓控释制剂的研究报道逐年增加,研究广度和深度显著加强,已成为当前中药制剂研究的前沿和热点。现就中药缓释制剂的研究进展情况、制剂的类型进行综述并提出研究思路,为中药缓控释制剂的研究和开发提供参考。     

Suppression of mouse skin tumor promotion and induction of apoptosis in HL-60 cells by Alpinia oxyphylla Miquel (Zingiberaceae)

There have been considerable efforts to search for naturally occurring substances for the intervention of carcinogenesis. Many components from dietary or medicinal plants have been identified that possess substantial chemopreventive properties. An example is curcumin (Curcuma longa Linn., Zingiberaceae), which has been shown to inhibit tumor promotion in experimental carcinogenesis. Alpinia oxyphylla Miquel, another plant of the ginger family used in oriental herbal medicine, contains diarylheptanoids whose structures are analogous to that of curcumin. In the present study, we have tested A.oxyphylla for its ability to suppress tumor promotion. Thus, topical application of the methanolic extract of dried fruits of A.oxyphylla significantly ameliorated 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced skin tumor promotion as well as ear edema in female ICR mice. In another study, treatment of HL-60 cells with the methanolic extract of A.oxyphylla significantly reduced the viability of the cells and also inhibited DNA synthesis. Microscopic examination of the treated cells showed characteristic morphology of apoptosis. Furthermore, cells treated with the extract of A.oxyphylla exhibited internucleosomal DNA fragmentation in time- and concentration-dependent manners. TPA- stimulated generation of superoxide anion in differentiated HL-60 cells was also blunted by A.oxyphylla. Taken together, these findings suggest that A.oxyphylla possesses potential chemopreventive and antitumorigenic activities.      

对陈香露白露片检验中有关问题的探讨

目的：探讨部颁标准陈香露片检验中存在的问题。方法：Ｅ和部颁标准和改进方法对陈香露片中的铋盐进行定性一定量、镁盐进行定性。结果：该片中的铋盐磺四鉴别反应。用稀硝酸代替盐酸做溶剂,呈正反应、镁盐氯化铵＝磷酸氢二钠鉴别反应,加氨试液后,析出的白色沉淀不足Ｍｇ（ＯＨ）２,而是Ｂｉ（ＯＨ）３,该深沉在氯化铵试液中不溶,干扰镁盐鉴别;铋盐含量测定项,用加抗坏血酸掩蔽剂的方法,排除了Ｆｅ＾３＋的干扰,使终点颜色     

The action of topical application of Vitamin B12 ointment on radiodermatitis in a porcine model

Radiodermatitis is an inevitable side effect of radiotherapy in cancer treatment and there is currently no consensus on effective drugs for treating the condition. Vitamin B₁₂ is known to be effective for repairing and regenerating damaged skin. However, there are few studies on the use of Vitamin B₁₂ for treating radiodermatitis. This study explored the therapeutic efficacy and mechanism of action of Vitamin B₁₂ ointment on radiodermatitis. A porcine model of grade IV radiodermatitis was established. The ointment was applied for 12 weeks after which histological staining, transmission electron microscopy, RT-qPCR, western blotting, and gene sequencing were performed for the evaluation of specific indicators in skin samples. After 12 weeks of observation, the Vitamin B₁₂ treatment was found to have significantly alleviated radiodermatitis. The treatment also significantly reduced the expression levels of NF-κB, COX-2, IL-6, and TGF-β in the skin samples. The pathways involved in the effects of the treatment were identified by analysing gene expression. In conclusion, Vitamin B₁₂ ointment was found to be highly effective for treating radiodermatitis, with strong anti-radiation, anti-inflammatory, and anti-fibrosis effects. It is thus a promising drug candidate for the treatment of severe radiodermatitis.