慢性重型肝炎患者血清肝细胞生长因子浓度变化的动态观察

研究慢性病毒性肝炎患者血清中肝细胞生长因子(HGF)的浓度变化与病情的关系,用ELISA法检测19例慢性乙型重型肝炎患者(慢重肝)血清中的HGF浓度的动态变化,并比较生存患者和死亡患者的HGF变化,结果:1.慢重肝患者血清HGF浓度变化与血清TBil的变化相一致,生存患者HGF在达到一个峰值后随血清TBil的降低而降低;死亡患者的HGF则进行性升高,在死亡前达最高值。2.生存患者的HGF峰值浓度为(5.214±1.657)μg/L,TBil为(236±109)μmol/L,PTA为(48.26±10.15)％;而死亡患者分别为(7.521±2.152)μg/L、(412±128).μmol/L、(30.09±14.28)％,均有显著性的差异。HGF在慢重肝患者血清中有较明显的升高,而且与TBil的变化有相关性,动态观察血清HGF的变化可作为判断病情的指标之一。     

上海市不同人群人芽囊原虫感染调查

目的了解上海市不同人群人芽囊原虫感染情况。方法在上海市6个区收集居民新鲜粪便,采用Ringer溶液37℃恒温条件下培养人芽囊原虫,计算人芽囊原虫感染率。结果检查居民2 984人,人芽囊原虫阳性者88人,感染率2.95%。上海市各区人群感染率间差异无统计学意义(χ~2=9.90,P>0.05);青浦区男性感染率为5.56%,女性感染率1.57%,差异有统计学意义(χ~2=5.38,P<0.05),其他5个区男、女性感染率差异无统计学意义(χ~2=0.08～0.54,P均>0.05);农民感染率为6.87%,与其他职业人群比较差异有统计学意义(χ~2=18.57,P<0.05)。结论上海市人群对人芽囊原虫普遍易感,以农民感染率最高。体外培养法敏感性较高.可用于人芽囊原虫感染的流行病学调查。     

Effect of cardiac output reduction on rate of desaturation in obstructive apnea.

The nadir of SaO2 during an obstructive apnea is dependent upon the apnea's duration and the rate of fall of saturation (dSaO2/dt). We postulated that a low Q, such as in patients with congestive heart failure with sleep apnea, or a reduction in Q, as seen in some humans during obstructive sleep apnea, might steepen dSaO2/dt. The mechanism postulated was lowering of SvO2 with increased pulmonary capillary blood oxygen uptake and faster depletion of alveolar oxygen. This study examines dSaO2/dt following the onset of apnea in eight spontaneously breathing adult baboons. Nonrepetitive obstructive apneas (30, 45, and 60 seconds) were created by clamping an indwelling cuffed endotracheal tube at the end of expiration. Following baseline measurements, the animals were given a bolus of a rapid-acting beta-adrenergic blocker followed by continuous infusion to reduce cardiac output and to limit the cardiovascular response to obstructive asphyxia. Fiberoptic catheters were used for continuous monitoring of SaO2, SvO2, and cardiac output. Esophageal pressure and relative thoracic gas volume (Respitrace) were monitored to insure equivalence of lung volume at the onset of apnea. Beta-adrenergic blockade reduced resting Q by a mean of 25 percent. The blocked vs unblocked dSaO2/dt was 0.73 vs 0.72 percent/s, 0.76 vs 0.73 percent/s, and 0.70 vs 0.71 percent/s for 30-second, 45-second, and 60-second apneas, respectively. Thus, mean dSaO2/dt for all durations of apneas was unaffected by beta-adrenergic blockade. We concluded that dSaO2/dt is not influenced by limited Q preceding or induced by obstructive asphyxia.     

嗜人按蚊迁飞可能性的研究Ⅰ "同期突增"和"同期突发"现象的初步观察

目的观察嗜人按蚊是否存在"同期突增"和"同期突发"现象,以期探讨嗜人按蚊存在迁飞特性的可能性. 方法在浅山区约2 km2范围内,选择位于不同山沟内相互隔离的3个自然村,采用室内全夜灯光诱捕法,观察嗜人按蚊、中华按蚊和三带喙库蚊是否存在"突增突发"现象. 结果嗜人按蚊和三带喙库蚊均存在"突增突发"现象;中华按蚊未出现这种现象. 结论嗜人按蚊存在迁飞特性的可能性.     

抗日本血吸虫单克隆抗体的制备及其在诊断中的应用

The present paper reported on an anti-CCA monoclonal antibody, McAb-IIID 10, which could be used in determinations of both parasite-oriented circulating antigens and specific anti-CCA antibodies. The established competitive ELISA (C-ELISA) using McAb-IIID 10 to detect schistosome-antibodies showed high sensitivity and specificity in the diagnosis of schistosomiasis with few cross-reactions. In field trials, coincident rates in 3 separate batches of serum samples when subjected to double-blind detections were obtained. A total of 1,915 serum samples had been determined by C-ELISA, among them 113 acute cases achieved a 100% positive rate, 765 chronic and 25 late cases showed 96.3% and 72% positive respectively. 70% of the 66 cured schistosomiasis cases turned to be negative. None of the 750 normal individuals showed positive reactions. No cross reaction was found in 27 sera from hydatidosis, whereas 1 and 2 positive reactions were found in 43 paragonimiasis sera and 126 clonorchiasis sera respectively. The established McAb-IIID 10 involved Dot-ELISA was found of value in the assessment of effective chemotherapy and showed a high negative conversion rate of 97.9% in 48 cured schistosomiasis patients. In 16 experimentally infected rabbits, 12 became negative in Dot-ELISA determinations at the 8th week post treatment, and the remaining 4 treated ones, the titer as well as the reaction intensity were also found reduced. A good coincidence rate was also found between C-ELISA and Dot-ELISA, their detection results may be complementary each other.     

Function of HAb18G/CD147 in invasion of host cells by severe acute respiratory syndrome coronavirus

To identify the function of HAb18G/CD147 in invasion of host cells by severe acute respiratory syndrome (SARS) coronavirus (CoV), we analyzed the protein-protein interaction among HAb18G/CD147, cyclophilin A (CyPA), and SARS-CoV structural proteins by coimmunoprecipitation and surface plasmon resonance analysis. Although none of the SARS-CoV proteins was found to be directly bound to HAb18G/CD147, the nucleocapsid (N) protein of SARS-CoV was bound to CyPA, which interacted with HAb18G/CD147. Further research showed that HAb18G/CD147, a transmembrane molecule, was highly expressed on 293 cells and that CyPA was integrated with SARS-CoV. HAb18G/CD147-antagonistic peptide (AP)-9, an AP of HAb18G/CD147, had a high rate of binding to 293 cells and an inhibitory effect on SARS-CoV. These results show that HAb18G/CD147, mediated by CyPA bound to SARS-CoV N protein, plays a functional role in facilitating invasion of host cells by SARS-CoV. Our findings provide some evidence for the cytologic mechanism of invasion by SARS-CoV and provide a molecular basis for screening anti-SARS drugs.     

丹参通脉胶囊对脑缺血再灌注损伤大鼠的作用

目的 观察丹参通脉胶囊对脑缺血再灌注大鼠的影响,探究其脑组织恢复程度。方法 取SD大鼠40只,制备脑缺血再灌注损伤模型（MCAO）,实验大鼠随机分为假手术组、模型组、血塞通组及丹参通脉胶囊组,观察脑缺血再灌注24 h后大鼠神经行为学评分、脑组织含水量、脑梗死体积、局部脑皮层血流量（rCBF）和局部微血管内皮细胞的变化情况。结果 与模型组及血塞通组比较,丹参通脉胶囊能显著降低大鼠的神经行为学评分（P<0.01）;降低脑组织含水量（P<0.01）;减小脑梗死体积（P<0.01）;提高局部脑组织血流量（P<0.01）;增加微血管内皮细胞的数量（P<0.01）。结论 丹参通脉胶囊对大鼠脑缺血再灌注损伤具有保护作用,可加速脑组织恢复。