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排序方式: 共有719条查询结果,搜索用时 31 毫秒
711.
Puri RK; Leland P; Obiri NI; Husain SR; Kreitman RJ; Haas GP; Pastan I; Debinski W 《Blood》1996,87(10):4333-4339
We have previously shown that human renal cell carcinoma (RCC) cells express large numbers of interleukin-13 receptors (IL-13R), a newly described hemopoietic growth factor receptor. To target tumor cells that express IL-13R, we have produced a chimeric protein composed of human IL-13 and a derivative of Pseudomonas exotoxin A, termed PE38QQR. We report here that IL13-PE38QQR is highly cytotoxic to many human RCC cell lines. IL-13R-negative cell lines or cell lines expressing low numbers of IL-13R ( < 300 sites/cell) that include human bone marrow- derived cells were not susceptible to the cytotoxic effect of IL 13- PE38QQR. The sensitivity of RCC cells to IL13-PE38QQR correlated positively with the density of IL-13R. The cytotoxic activity of IL13- PE38QQR was competed by an excess of IL-13 in a protein synthesis inhibition assay and confirmed by a clonogenic assay. Even though IL-13 and IL-4 are homologues and IL-4R and IL-13R have been proposed to share a receptor subunit, IL-4 did not compete for the cytotoxicity mediated by IL13-toxin on RCC. IL13-PE38QQR competes for [125I]-IL-13 binding sites on RCC cells, although at a lower affinity than the wild- type recombinant cytokine. Human T-cell, B-cell, and monocytic cell lines are unresponsive to the cytotoxic action of IL13-PE38QQR. Thus, our results indicate that IL13-PE38QQR is highly cytotoxic to human RCC cells, although it is not cytotoxic to a variety of normal hematopoietic cells. IL13-PE38QQR should be further investigated preclinically for the treatment of human RCCs. 相似文献
712.
Analysis of antibodies against components of the autonomic nervous system in diabetes mellitus 总被引:1,自引:0,他引:1
Stroud CR; Heller SR; Ward JD; Hardisty CA; Weetman AP 《QJM : monthly journal of the Association of Physicians》1997,90(9):577-585
Antibodies to autonomic nervous system structures have previously been
detected using a complement fixation immunofluorescence test in the sera of
patients with insulin-dependent diabetes mellitus (IDDM) and non-insulin
dependent diabetes mellitus (NIDDM). These antibodies might play a role in
the aetiology of autonomic neuropathy. Sera from 45 IDDM, 40 NIDDM and 52
control subjects were tested by immunofluorescence for antibodies to human
sympathetic ganglia, human adrenal medulla and rabbit vagus nerve. The use
of human sympathetic ganglia was compared with rabbit tissue for the
detection of sympathetic ganglia antibodies; the results for these
autonomic nervous system antibodies were also compared with results using
an ELISA. There was no relationship between the presence of antibodies
detected by ELISA and those detected by immunofluorescence, but of 14 IDDM
patients with thyroid antibodies, 12 had autonomic nervous system
antibodies detected by either immunofluorescence or ELISA (p < 0.005
compared to patients without thyroid antibodies). To further characterize
the autoantigen(s), immunoblotting was performed. An adrenal antigen
corresponding to 74 kDa was detected in sera from three patients, only one
of whom had antibodies detectable by ELISA and immunofluorescence. One IDDM
serum showed specific binding to a vagus nerve antigen corresponding to 33
kDa. No specific binding to sympathetic ganglia antigen was demonstrated.
Antibodies against autonomic nervous system antigens are an inconsistent
feature of diabetes, and appear more associated with coincidental
autoimmunity against other organs such as the thyroid.
相似文献
713.
M Psichogiou ; A Katsoulidou ; E Vaindirli ; B Francis ; SR Lee ; A Hatzakis 《Transfusion》1997,37(8):858-862
BACKGROUND: The study of the sensitivity of screening assays is greatly facilitated by testing the sequential changes in seroconverting individuals. The aim of this study was to investigate the early immunologic response after hepatitis C virus (HCV) infection and to evaluate whether HCV envelope (E2) recombinant antigen would provide a significant increase in sensitivity for detection of anti-HCV. STUDY DESIGN AND METHODS: Twenty hemodialysis patients who were seroconverting to anti-HCV were included in this study. They were followed up for a mean period (+/− SD) of 10.5 +/− 3.3 months, in which 13 to 46 serum samples per case were collected. Each sample was tested for anti-HCV by second- and third-generation enzyme immunoassay (EIA-2 and EIA-3) and recombinant immunoblot assay (RIBA-3). E2 antibodies were tested by a prototype EIA in which E2 was expressed as a recombinant antigen in Chinese hamster ovary cells. RESULTS: Alanine aminotransferase elevation was observed in 18 of 20 cases. Reactivity against c100, c33c, c22, NS5, and E2 was detected in 15 (75%), 19 (95%), 15 (75%), 2 (10%), and 17 (85%) patients, respectively; c33c was the most immunogenic antigen, followed in descending order by E2, c22, c100, and NS5. E2 antibody reactivity resolved the two RIBA-3- indeterminate cases. However, there was no case in which E2 reactivity preceded all other HCV antigens. Anti-E2 was found to react in all patients of genotypes 1a, 1b, and 3a but in only 2 of 4 patients of genotype 4a. CONCLUSION: In this group of seroconverting individuals, E2 antigen was shown to be highly immunoreactive and did resolve some RIBA-3-indeterminate samples as being positive, on the basis of reactivity to multiple antigens, but it did not improve early detection of seroconversion. 相似文献
714.
高效液相色谱法同时测定盐酸维拉帕米及其主要代谢产物 总被引:6,自引:0,他引:6
建立了反相高效液相色谱法同时测定人血浆中维拉帕米及其主要代谢产物去甲维拉帕米血药浓度.以甲醇—水—三乙胺(67∶33∶0.4,pH6.7)为流动相,乙吗噻嗪(ethmosine)为内标,样品用正己烷—正丁醇混合液提取浓缩后进样,紫外检测器检测(279nm)。此法操作简便,精密度好,日内、日间误差:维拉帕米<8.6%,去甲维拉帕米<7.6%;方法回收率高,维拉帕米、去甲维拉帕米回收率均>92%。两者血药浓度在25~1000ng·ml-1范围内呈线性关系,最小检测浓度维拉帕米:2.5ng·ml-1,去甲维拉帕米:5.0ng·ml-1。应用该法测定了6名志愿者口服盐酸维拉帕米片剂后的血药浓度。 相似文献
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