ICP—MS法测定藏药智托洁白丸中微量元素

目的：建立藏药智托洁白丸中微量元素含量的测定方法。方法：样品经微波消解后,以铑为内标,用电感耦合等离子体质谱（ICP—MS）测定样品中Cr、Mn、Ni、Co、Cu、Zn、As、Pb、Fe、Se等10种微量元素的含量。结果：该方法对各元素的检出限为0．003-0．093μg·g^-1,加标回收率在96-106％之间,国家标准物质茶标（GBW07404）和杨树叶（GBW07405）的测定值与标准值基本吻合。结论：方法快速、准确、灵敏度高,可以用于测定智托洁白丸中微量元素的含量。     

三维适形放疗结合252锎中子腔内后装照射治疗中晚期食管癌的临床研究

目的:探讨三维适形放疗结合锎(252Cf)中子腔内后装照射治疗中晚期食管癌的疗效、不良反应及晚期并发症。方法:92例中晚期食管癌患者全部采用三维适形放疗结合锎(252Cf)中子腔内后装照射治疗。三维适形放疗总剂量为45Gy-54Gy,分25-30次,5-6周完成;腔内照射3-4Gy/次,1次/周,共2-4次,总吸收剂量10Gy-16Gy。结果:治疗结束3个月进食梗阻缓解率为95%。近期疗效:完全缓解(CR)27%,部分缓解(PR)69.6%。1、2、3年局部控制率分别为82%、51%、32%;1、2、3年生存率分别为:78%、35%、18%;急性放射性食管炎发生率为54%;晚期食管狭窄发生率9%;食管穿孔发生率2%。结论:中晚期食管癌采用三维适形放疗结合锎(252Cf)中子腔内后装照射能够迅速而持久地缓解进食梗阻症状,提高局部控制率及生活质量,近期疗效显著,放疗不良反应未见明显增加。     

非晶体硒平板探测器的影像质量比较

目的 比较研究睿影WV3000T和HOLOGIC 2种非晶硒平板探测器的影像质量和解剖结构可见度.方法 分析不同厚度水模影像,评价2种探测器灵敏度,分析30对不同体位的临床影像,采用3点记分法进行观察者评分,并评价15个解剖结构的影像质量和可见度.结果 在2种非晶体硒平板探测器中,水模中铝梯和线对卡影像质量差异无统计学意义(P>0.05);低对比测试卡影像质量差异无统计学意义(P>0.05);临床医学影像质量差异无统计学意义(P>0.05).结论 睿影WV3000T和HOLOGIC非晶硒平板探测器的影像质量和解剖可见度相等.     

黑加仑提取物保护血管内皮细胞氧化损伤的实验研究

目的研究黑加仑提取物对过氧化氢所致血管内皮细胞ECV-304损伤的影响。方法建立体外过氧化氢损伤模型,测定细胞存活率、上清液中丙二醛(MDA)含量、乳酸脱氢酶(LDH)活力、一氧化氮(NO)、内皮素(ET)和前列环素(PGI2)含量,并运用流式细胞仪观察黑加仑提取物对过氧化氢对内皮细胞ECV-304凋亡的影响。结果黑加仑提取物各剂量都能显著提高H2O2损伤内皮细胞的存活率(P<0.05);显著抑制H2O2损伤引起内皮细胞的LDH和ET的释放(P<0.05);显著减少H2O2损伤的内皮细胞MDA的生成(P<0.05);增加H2O2损伤的内皮细胞NO和PGI2水平(P<0.05)。黑加仑提取物的各组、阳性对照组和正常对照组早期凋亡率和总凋亡率明显低于H2O2模型组(P<0.05)。结论黑加仑提取物对过氧化氢损伤的脐静脉内皮细胞ECV-304具有保护作用;黑加仑提取物可降低H2O2诱导的ECV-304凋亡的早期凋亡率和总凋亡率,以达到保护内皮细胞的作用。     

Cervical stimulation activates A1 and locus coeruleus neurons that project to the paraventricular nucleus of the hypothalamus

In female rats, stimulation of the uterine cervix during mating induces two daily surges of prolactin. Inhibition of hypothalamic dopamine release and stimulation of oxytocin neurons in the paraventricular nucleus (PVN) are required for prolactin secretion. We aim to better understand how stimulation of the uterine cervix is translated into two daily prolactin surges. We hypothesize that noradrenergic neurons in the A1, A2, and locus coeruleus (LC) are responsible for conveying the peripheral stimulus to the PVN. In order to determine whether projections from these neurons to the PVN are activated by cervical stimulation (CS), we injected a retrograde tracer, Fluoro-Gold (FG), into the PVN of ovariectomized rats. Fourteen days after injection, animals were submitted to artificial CS or handling and perfused with a fixative solution. Brains were removed and sectioned from the A1, A2, and LC for c-Fos, tyrosine hydroxylase (TH), and FG triple-labeling using immunohistochemistry. CS increased the percentage of TH/FG+ double-labeled neurons expressing c-Fos in the A1 and LC. CS also increased the percentage of TH+ neurons expressing c-Fos within the A1 and A2, independent of their projections to the PVN. Our data reinforce the significant contributions of the A1 and A2 to carry sensory information during mating, and provide evidence of a functional pathway in which CS activates A1 and LC neurons projecting to the PVN, which is potentially involved in the translation of CS into two daily prolactin surges.