轻、中度缺氧对事件相关电位的影响及不同刺激模式的比较

目的 观察轻、中度缺氧对脑事件相关电位（ＥＲＰ）的影响,比较不同刺激模式发的ＥＲＰ对缺氧的敏感性,方法１２名受试者在地面、２５００ｍ和４３００ｍ匠心同度（吸放低氧混合气）分别进行视觉Ｏｃｄｄｂａｌｌ、Ｓｔｅｒｎｂｅｒｇ记忆量（ＭＳＥＴ）为１ 和ＭＳＥＴ为３的任务测试,记录ＥＲＰ的Ｐ３潜伏期、波幅和反应时（ＲＴ）及反应错误率（ＥＲ）。结果 ４３００ｍ高度缺氧时Ｐ３潜伏期显著延长,任务难度较大时ＥＲ增     

Cancer-associated retinopathy

Patients with systemic cancer may have a variety of ocular complaints. Most commonly these are metastases or adverse effects of therapy. Paraneoplastic syndromes, like cancer-associated retinopathy, rarely cause ophthalmic symptoms. We describe a patient with a malignant mixed mullerian tumor and cancer-associated retinopathy who had circulating serum antibodies to recoverin and cells positive for recoverin in the tumor. We discuss the typical clinical symptoms as well as the pathophysiology of this uncommon disorder.     

喉鳞状细胞癌组织体外原代培养的初步研究

目的 应用体外培养技术,对喉鳞状细胞癌组织进行体外培养,探讨喉鳞癌组织原代培养中的各种影响因素,为建立人喉鳞癌组织的细胞系提供实验基础。方法采用体外组织培养技术,对24例人喉鳞状细胞癌组织进行原代培养,观察原代培养中肿瘤细胞的生长与供体的年龄、肿瘤组织的分化程度及不同培养方法的关系,分析在人喉鳞癌细胞的培养中成纤维细胞、微生物污染的影响。结果 24例人喉鳞状细胞癌组织标本,年龄小于60岁组的细胞生长率为31.25％(5/16例),年龄大于60岁组为37.5％(3/8例);高分化组为100％(2/2例),中分化组为30.8％(4/13例),低分化组25％(2/8例);组织块培养法为43.75％(7/16例),酶消化法培养为10％(1/10例);倒置显微镜下观察,在培养的第5～7天,在贴壁组织块周围可见到有上皮样细胞爬出。全部标本中,成纤维细胞的过度生长和微生物的污染是阻碍人喉鳞癌细胞生长的重要因素。结论培养组织的细胞生长率与供体的年龄关系不大;肿瘤组织的分化程度较高者,细胞的生长率较高;与酶消化分离培养法相比,贴壁组织块培养法的细胞生长率较高;成纤维细胞及微生物的污染是阻碍人喉鳞癌细胞系建立的重要因素。     

钾通道阻滞剂对大鼠支气管平滑肌细胞增殖的影响

目的探讨电压依赖性延迟整流钾通道(K_V)、Ca²⁺激活钾通道(K_Ca)和ATP敏感性钾通道(K_ATP)对大鼠支气管平滑肌细胞(BSMC)增殖的影响。 方法应用免疫细胞化学、MTT微量比色分析法及流式细胞术,观察K_V,K_Ca和K_ATP对培养中大鼠BSMC增殖的影响。结果K_V阻断剂4-氨基吡啶(4-AP)显著促进大鼠BSMC增殖细胞核抗原的表达,提高BSMC吸光度值,使S+G₂M期细胞数显著增多,并显著提高基础状态下BSMC内Ca²⁺浓度,而K_Ca阻断剂四乙铵(TEA)和K_ATP阻断剂格列本脲(Glib)均无此效应。 结论大鼠BSMC K_V活性的抑制,可提高细胞内Ca²⁺浓度,促进细胞的增殖,而K_Ca和K_ATP对BSMC的增殖均无明显影响。     

反相高效液相色谱法测定托拉噻米胶囊中托拉噻米的含量

目的建立高效液相色谱法测定托拉噻米胶囊中托拉噻米的含量。方法以岛津Sh impack CLC(M)C18(250 mm×4.6 mm,5μm)为色谱柱,以甲醇-0.02 mol.L-1磷酸二氢钾溶液(用磷酸调节pH至3.0)(65∶35)为流动相,检测波长为UV291 nm,流速为0.9 ml.min-1。结果托拉噻米在2~24μg.ml-1范围内具有良好线性关系(r=0.999 9),平均加样回收率为99.73%,相对标准差为0.17%;三批样品的标示百分含量(%)分别为99.8、100.1及100.4。结论本方法准确、灵敏度高、重现性好,可用于托拉噻米胶囊的含量测定。     

巨噬细胞泡沫化抑制剂的研究进展

胆固醇酯蓄积的巨噬细胞(泡沫细胞)是早期动脉粥样硬化斑块的主要组成和特征。它通过其细胞表面的多种受体无限制地摄取氧化型低密度脂蛋白(ox-LDL)颗粒及其他配体,结果在巨噬细胞内有大量的胆固醇酯和三酰甘油蓄积,使巨噬细胞发展成为泡沫细胞。因此,通过各种途径抑制这种在巨噬细胞的过量脂质蓄积,可以起到预防和治疗动脉粥样硬化的作用。     

STRUCTURAL CHARACTERISATION OF AN IMMUNOMODULATING POLYSACCHARIDE ISOLATED FROM AQUEOUS EXTRACT OF PLEUROTUS FLORIDA FRUIT-BODIES

The water-soluble glucan was obtained from Pleurotus florida fruit bodies by hot water extraction, ethanol precipitation, DEAE cellulose dialysis and Sephadex G-75 gel filtration. The structural information of the glucan was achieved by chemical (hydrolysis, methylation, periodate oxidation) and spectroscopic (¹H and ¹³C) analyses, indicated a repeating unit built up of (1→6)-linked D-glucose. The following structure has been determined for the repeating unit: →6)-α-D-Glcp-(1→ This fraction exhibited significant macrophage activity through the release of nitric oxide     

枳术汤对脾虚便秘小鼠结肠肥大细胞与胃肠激素的影响

目的观察枳术汤对脾虚便秘小鼠结肠粘膜肥大细胞(MC)的影响及其与P物质(SP)、生长抑素(SS)免疫反应阳性的相关性,探讨该方治疗脾虚便秘的可能作用机制.方法采用饥饱失常和过度疲劳配合燥结便秘的方法复制小鼠脾虚便秘模型,甲苯胺蓝改良法及LSAB免疫组织化学标记法进行MC和SP、SS的检测,用IMS型彩色图像系统和免疫组化分析软件进行分析.结果脾虚便秘模型小鼠结肠粘膜肥大细胞密度明显降低;其MC密度与结肠粘膜SP免疫反应阳性强度均值(r=0.6508,P＜0.01)、面积(r=0.684,P＜0.01)呈正相关;而与SS免疫反应阳性强度均值(r=-0.7568,P＜0.01)、面积(r=-0.683,P＜0.01)呈负相关.大、中剂量的枳术汤能使脾虚便秘小鼠结肠粘膜MC密度增加,并一定程度上使SP免疫反应阳性增强,SS的免疫反应阳性减弱.结论调节肠壁粘膜SP、SS免疫反应阳性强度,影响MC释放5-HT、组胺从而调整胃肠运动功能,这可能是枳术汤治疗脾虚便秘的机制之一.     

Day-case local anaesthetic radiofrequency thermal ablation of benign prostatic hyperplasia: a four-year follow-up

OBJECTIVE: This study reports long-term (median 4 years) clinical effectiveness, safety and patient acceptance of transurethral ablation prostatectomy (TURAPY) for symptomatic benign prostatic enlargement (BPE) using a radiofrequency technique. MATERIAL AND METHODS: 25 men were treated as day-case procedures under local anaesthesia for 1 h. The age range was 55-88 years (mean age 65.5 years) and all were suffering from symptomatic BPE with urinary flow rates of 12 ml/s or less. The treatment was carried out with a computer-based device using a special heating element mounted on a Foley-like catheter for prostatic ablation and the temperatures were monitored continuously in the prostatic region, sphincteric area and rectum for safety purposes. RESULTS: TURAPY was carried out in all patients with temperatures ranging from 70 to 82 degrees C depending on the tolerance of the patient. The post-treatment mortality was nil and only 19 patients complained of mild dysuria, passing of some debris and/or minor bleeding on voiding which settled spontaneously in 2-3 weeks, except in two patients who had proven urinary infection requiring antibiotic therapy. At 4 years the International Prostate Symptom Score (I-PSS) improved from a mean value of 16.0 to 7.2 (p < 0.01) with quality-of-life score falling from 4 to 1.9 (p < 0.05); mean flow rate increased from 8.5 to 11.7 ml/s and mean residual volume decreased from 185.04 to 52.8 ml (p < 0.05). Post-treatment pressure flow studies in 14 patients showed normal pressure voiding in one, with four being in an equivocal range, but the rest were obstructed. Prostate ultrasound scanning before and after treatment in 15 patients showed a mean reduction of volume of 16.7 ml suggesting cavitation of prostate following TURAPY. CONCLUSIONS: TURAPY provides lasting subjective but modest objective benefits in symptomatic BPE patients. It is worthy of consideration in elderly patients, or in those who are unfit for surgery or who do not wish to undergo surgery. It is a safe procedure with little transient morbidity but no mortality.     

从玄府气液理论探讨偏头痛的病机及治疗

偏头痛是一种常见的神经-血管功能障碍性疾病。玄府是遍布机体各处的一种至微至小的组织结构,其主要功能包括流通气液、渗灌气血和运转神机。脑玄府开阖不利是头痛各种证候形成的共同基础,气郁玄府、浊停玄府、血瘀玄府或玄府气血不足,是头痛重要的发病机制。治以开通玄府法,以畅达人体阻滞之气、血、津、液,用药多选用祛风、理气、活血、化痰、通络之品。