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471.
CR de Andrade PF Leite AC Montezano DA Casolari A Yogi RC Tostes R Haddad MN Eberlin FRM Laurindo HP de Souza FMA Corr��a AM de Oliveira 《British journal of pharmacology》2009,157(4):568-580
Background and purpose:
There are interactions between endothelin-1 (ET-1) and endothelial vascular injury in hyperhomocysteinemia (HHcy), but the underlying mechanisms are poorly understood. Here we evaluated the effects of HHcy on the endothelin system in rat carotid arteries.Experimental approach:
Vascular reactivity to ET-1 and ETA and ETB receptor antagonists was assessed in rings of carotid arteries from normal rats and those with HHcy. ETA and ETB receptor expression was assessed by mRNA (RT-PCR), immunohistochemistry and binding of [125I]-ET-1.Key results:
HHcy enhanced ET-1-induced contractions of carotid rings with intact endothelium. Selective antagonism of ETA or ETB receptors produced concentration-dependent rightward displacements of ET-1 concentration response curves. Antagonism of ETA but not of ETB receptors abolished enhancement in HHcy tissues. ETA and ETB receptor gene expressions were not up-regulated. ETA receptor expression in the arterial media was higher in HHcy arteries. Contractions to big ET-1 served as indicators of endothelin-converting enzyme activity, which was decreased by HHcy, without reduction of ET-1 levels. ET-1-induced Rho-kinase activity, calcium release and influx were increased by HHcy. Pre-treatment with indomethacin reversed enhanced responses to ET-1 in HHcy tissues, which were reduced also by a thromboxane A2 receptor antagonist. Induced relaxation was reduced by BQ788, absent in endothelium-denuded arteries and was decreased in HHcy due to reduced bioavailability of NO.Conclusions and implications:
Increased ETA receptor density plays a fundamental role in endothelial injury induced by HHcy. ET-1 activation of ETA receptors in HHcy changed the balance between endothelium-derived relaxing and contracting factors, favouring enhanced contractility.British Journal of Pharmacology (2009) 157, 568–580; doi:10.1111/j.1476-5381.2009.00165.x; published online 9 April 2009This article is part of a themed section on Endothelium in Pharmacology. For a list of all articles in this section see the end of this paper, or visit: http://www3.interscience.wiley.com/journal/121548564/issueyear?year=2009 相似文献472.
LI LH ZHANG R ZHOU CR 《中国生物医学工程学报(英文版)》2005,14(2):85-92
Because of the incomparable merits (nontoxicity, non-remainder, fast transfer mass) of supercritical carbon dioxide fluid technique(SC-CO2), it was used to developed a series of novel biodegradable tissue engineering scaffold materials in this research. The novel PLA/chitosan composite materials could be molded to different shapes, and the porosity of the materials were over 200 lam and connected. Chondrocyte cultivation, subcutaneous and intramuscular implantation were mainly discussed this paper. The results showed that the cells could well adhere, grow and multiplicate on the surface of the materials, which indicated good biocompatibility of the composite materials. The plantation test revealed that the PLA materials had already dismissed 2 month late in the body, while the composite materials could still keep certain strength and shape, and the most important things is the response of the tissue toward the implanted PLA/chitosan composite materials was mild and had far less inflammation than PLA materials. 8 to 16 weeks later, fiber membrane was stable; degradation of the materials was seen clear and tissue had already spread into it. 相似文献
473.
A familial case of X chromosome deletion ascertained by cytogenetic screening of women with premature ovarian failure 总被引:2,自引:2,他引:2
Davison RM; Quilter CR; Webb J; Murray A; Fisher AM; Valentine A; Serhal P; Conway GS 《Human reproduction (Oxford, England)》1998,13(11):3039-3041
The association between X chromosome deletions and premature ovarian
failure is well established. Previous anecdotal reports however, have not
documented the prevalence of X deletions in women with premature ovarian
failure. We therefore performed cytogenetic analyses on 79 women with
primary or secondary amenorrhoea to assess the utility of screening for a
genetic marker for familial premature ovarian failure. A normal karyotype
was found in 77 women. One woman with primary amenorrhoea had an XY
karyotype and a woman with secondary amenorrhoea had a deletion at Xq 26.1.
This second case had a family history of premature ovarian failure, and her
mother who underwent premature ovarian failure at 28 years shared this
deletion. The early diagnosis of familial X deletions causing premature
ovarian failure allowed for the prediction of impending menopause and the
implementation of manoeuvres to advance conception. Although cytogenetic
aberrations are rare in secondary amenorrhoea, the ability to predict
premature ovarian failure can be vital.
相似文献
474.
The mini-micro-epididymal sperm aspiration for sperm retrieval: a study of urological outcomes 总被引:1,自引:5,他引:1
Nudell DM; Conaghan J; Pedersen RA; Givens CR; Schriock ED; Turek PJ 《Human reproduction (Oxford, England)》1998,13(5):1260-1265
Epididymal sperm aspiration and in-vitro fertilization (IVF) with
intracytoplasmic sperm injection is an established treatment for
obstructive azoospermia. Sperm aspiration is performed with either an
incision or percutaneously. To control costs, minimize morbidity and retain
the advantages of both approaches, we developed a mini-incision technique
for epididymal aspiration and here report sperm retrieval and
procedure-related outcomes. Twenty-six consecutive patients with
obstructive azoospermia underwent epididymal sperm retrieval through a 1 cm
incision with local anaesthesia to provide spermatozoa for concurrent IVF
cycles. The quality of retrieved spermatozoa, the quantity of spermatozoa
cryopreserved as well as anaesthetic requirement, recovery time and patient
satisfaction were evaluated. Fresh epididymal spermatozoa were retrieved in
25 of 26 (96%) patients. In one patient, testicular sperm extraction was
necessary. Excess motile spermatozoa were cryopreserved in 24 of 26 (92%)
patients; a mean total motile count of 4.8x10(6) motile spermatozoa were
banked. The procedure was performed with 62% of patients receiving minimal
i.v. sedation. Post-procedure recovery was rapid, with a median time to
return to work of 2.0 days with a median of 2.0 pain pills taken.
Procedure-related satisfaction was high. The mini-micro-epididymal sperm
aspiration achieves the goals of reliable retrieval of abundant epididymal
spermatozoa with a single, minimally morbid procedure. It appears to
combine the advantages of the incision and percutaneous approaches.
相似文献