人睾丸cDNA文库的构建

本研究从人睾丸组织的总RNA中纯化mRNA,并以此为模板,在AMV反转录酶的作用下,制备互补的SS-cDNA。用RNaseH降解模板mRNA,经DNA多聚酶Ⅰ作用,合成ds-cDNA。用T_4DNA多聚酶制备cDNA的平末端,在T_4DNA连接酶的作用下,连接Ecorl接头并与λgt11载体重组连接,以大肠杆菌Y1090做受体菌转染,构建人睾丸cDNA文库。结果表明cDNA文库构建成功,将对生殖和避孕进一步研究发挥重要的作用。     

Glenoid labrum: preliminary work with use of radial-sequence MR imaging

The authors describe a magnetic resonance imaging method for examination of the glenoid labrum of the shoulder joint that utilizes a radial fast-imaging sequence. Seven shoulders were examined: a total of five in three healthy asymptomatic volunteers, one in a symptomatic patient not suspected of having a lesion of the glenoid labrum, and one in a patient with recurrent shoulder dislocation and surgical proof of an extensive tear of the labrum. The preliminary results suggest that this technique may advantageously demonstrate pathologic changes in the glenoid labrum and may contribute to the evaluation of the unstable and painful shoulder.