A Case of Liposarcoma of the Cheek

A rare case of liposarcoma of the left cheek in a 51-year-oldwoman is reported. The first biopsy specimen was indicativeof lipoma. After one year, the patient consulted us again, becauseof rapid increase in size of the tumor, and then, since malignancywas suspected, another biopsy was performed and a diagnosisof a well-differentiated liposarcoma was made. The tumor wasexcised, and radiation and chemotherapy were given in the operativecourse. After one year, no evidence of recurrence or metastasiswas observed.     

Reutilization of DNA Breakdown Products from Lymphocytes in Lumen of Intestine

Migration of a very large number of lymphocytes (211.8 x 10⁶ per day)into the intestinal canal of rats, which weighed about 100 Gm., was found.Lymphocytes in the lumen of the intestine were 80.2 per cent small, 15.9 percent medium and 3.9 per cent large. Any recycling of instilling cells intothe intestine could not be observed. Lymphocytes labeled with H³-thymidine,obtained from both thymus and mesenteric lymph nodes of donor rats, werewashed and injected into the intestine of recipient rats. H³ activity of the bloodand thoracic duct lymph plasma after administration of labeled lymphocytesshowed that DNA breakdown products from the lymphocytes in the gut wereabsorbed and transferred by way of both the portal vein and the thoracic duct.Evidence that the activity was actually incorporated into the DNA of proliferating cells of the recipient was demonstrated by autoradiographic means.

Submitted on July 15, 1965 Accepted on October 8, 1966     

Role of γδ T lymphocytes in the development of Behçet's disease

Phenotypic and functional properties of γδ T cells, which play an important role in mucocutaneous immunity, were examined to elucidate whether immunological abnormality in Behc¸et's disease may be related to a specific T cell population. We found that CD45RA⁺Vγ9⁺Vδ2⁺γδ T cells, which constitute a minor population of γδ T cells in healthy individuals, were increased in number in Behc¸et's disease irrespective of disease activity. This CD45RA⁺ subset of γδ T cells in the active, but not inactive, phase of this disease expressed IL-2Rβ and HLA-DR, suggesting that they are activated in vivo in active Behc¸et's disease. In addition, the CD45RA⁺γδ T cells produced extreme amounts of tumour necrosis factor and contained perforin granules. These data indicate that a phenotypically distinct subset of γδ T cells, CD45RA⁺CD45RO⁻Vγ9⁺Vδ2⁺, may contribute to immunological abnormalities which may lead to complexity of pathophysiology in Behc¸et's disease.     

Etched-Porcelain Resin-Bonded Onlay Technique for Posterior Teeth

ABSTRACT: porcelain to enamel and dentin. This evolution has dramatically changed restorative dentistry and has produced a new, highly esthetic, and strong restoration-etched-porcelain resin-bonded restorations. These restorations can be placed with confidence on posterior teeth. They are virtually indistinguishable from natural tooth structure and, thus provide additional treatment alternatives for patients who desire natural-appearing posterior teeth. Etched-porcelain resin-bonded onlays on posterior teeth not only are an excellent restorative modality for moderately broken-down teeth but also may be used to bring caries-free teeth into occlusion. The latter can be an option for stabilizing occlusion for some patients with posterior open bite due to various causes. This article presents clinical and laboratory techniques for etched-porcelain resin-bonded onlays on posterior teeth. Remarkable advances in adhesive dentistry have made it possible to strongly bond     

脑缺血再灌注损伤后RGMb表达的变化及其对轴突生长的作用

目的探讨大鼠脑缺血再灌注损伤后RGMb(repulsive guidance molecule b)表达的变化及RGMb在体外对轴突生长的作用。方法①取出生后7-9d的Wistar大鼠提取内嗅皮层(entorhinal cortex,EC)细胞,分为:正常对照组和RGMb(10μg/ml)处理组,采用细胞培养和免疫荧光染色等方法,检测在体外培养中RGMb处理的新生大鼠EC细胞轴突长度的变化。②取正常8w的Wistar大鼠10只,随机分为:假手术组和脑缺血再灌注48h组,各5只。采用Western blot方法检测脑缺血再灌注48h后海马组织中RGMb表达的变化。结果①在体外培养中RGMb(10μg/ml)能抑制新生大鼠EC细胞轴突的生长。②脑缺血再灌注48h后成年大鼠海马组织中RGMb的表达明显上调。结论脑缺血再灌注损伤后RGMb表达明显增加,提示RGMb可能参与脑缺血再灌注损伤后的神经再生障碍、炎症和细胞凋亡等病理生理过程。     

Clinical Evaluation of Endoscopic Gastric Mucosal Resection

Abstract: Endoscopic gastric mucosal resection (EGMR), by which a large specimen can be obtained, has been developed for the diagnosis and treatment of the borderline lesion (B. L.) or early gastric cancer (EGC), since biopsy specimens obtained by conventional techniques are too small to make an accurate diagnosis in number of cases. Thirty-eight lesions in 35 cases (26 lesions in 23 cases with B. L. and 12 lesions in 12 cases with EGC) were resected for the purpose of treatment by EGMR, and 24 lesions (6 submucosal tumors, 3 B. L. s, 14 cases of EGC and 1 malignant lymphoma) for the purpose of diagnosis. By means of histological examination of the bite biopsy specimens following EGMR, twenty-three lesions in 26 cases (89%) with a B. L. and 8 in 12 cases (67%) with EGC for treatment by EGMR were found to have been completely resected. After further histological examination of EGMR specimens obtained for diagnosis, one out of 3 B. L. s was diagnosed as EGC (IIa) and the others as bengin lesions. Out of 14 EGC cases, 6 were diagnosed as carcinoma, 3 as B. L. s and 5 as benign lesions. In Group III lesions obtained by conventional endoscopic biopsy, 8 out of 40 lesions (20%) were defined as EGC, 28 lesions (70%) as a B. L. and 4 lesions (10%) as benign lesions respectively by means of histological examination of the EGMR specimens. Although obvious side effects were observed in 4 cases, these patients could be treated by endoscopic management or drug therapy. It can be concluded that EGMR, by which large specimens can be obtained, is a useful procedure for the purpose of diagnosing and treating gastric mucosal lesions without causing major risk.     

Identification of the epidural space in children

We have used an intravenous micro-drip infusion set to identify the epidural space in 350 infants and children. The infusion set was prepared with saline and connected to the hub of an epidural needle. Free dripping of saline was regarded as a sign that the needle tip had entered the epidural space. The overall success rate of this method was 97.7%.     

Significant changes in intestinal lymphatic system and immune response elicited by Peyer's patch excision in adult rats

Abstract The effect of deprivation of Peyer's patches (PP) on transport of lymphocytes through intestinal lymph and intestinal mucosal immune responses was investigated in rats. All visible PP in the rat small intestine were excised in order to examine the roles of PP in the intestinal lymphatic system and mucosal immune responses of the intestine. Two weeks after the experimental excision of PP, lymphocyte transport in intestinal lymph was significantly decreased in PP-excised rats without significant changes in lymphocyte subsets as compared with sham operated control rats. Lymphocyte subsets as determined morphometrically in the intestinal mucosa showed no significant alteration in PP-excised rats. There was a significant decrease in the number of immunoglobulin A (IgA) containing cells in the intestinal mucosa of PP-excised rats, while IgM and IgG containing cells showed no statistically significant changes in number. Conversely, the macrophages in the intestinal mucosa increased in number, suggesting the enhanced accessory functions of these macrophages. Antigen-specific immune response was further studied in PP-excised rats using intraduodenal priming and challenge with cholera toxin (CT). Both the determinations of cells producing antigen-specific antibody in the intestinal mucosa using anti-CT antibody and those of cells secreting anti-CT Ig in the intestinal lymph by enzyme-linked immunospot (ELISPOT) assay showed a significant reduction of CT-specific antibody production in PP-excised rats compared with controls. Peyer's patches appear to have an important role in lymphocyte transportation through intestinal lymph and also in mucosal immune responses.