Resveratrol accelerates wound healing by attenuating oxidative stress-induced impairment of cell proliferation and migration

BackgroundImpaired wound healing, which is due to various external and internal factors that are involved in wound pathophysiology, leads to high rates of morbidity and mortality worldwide. Oxidative stress injury is an important factor that affects wound healing by changing the whole healing process. So, resveratrol, a dietary fruits polyphenol, which is known for its antioxidant properties, maybe the candidate to accelerate the wound-healing process.MethodsThe Human Umbilical Vein Endothelial Cells (HUVECs) was used for in vitro experiments to evaluate the effect of resveratrol on hyperglycemia-induced gene expression, oxidative stress and cell proliferation. The diabetic rat model was used to evaluate the effect of resveratrol on cutaneous burn injury healing process.ResultsIncreases in H₂O₂ decreased cell viability with the 0?800 μM concentration range, and resveratrol could protect HUVECs against H₂O₂-induced injury. The scratched wound closed rate in H₂O₂ group was significantly smaller than the Control group (p < 0.05) and Resveratrol + H₂O₂ group (p < 0.05). The fluorescence intensity of ROS was lower in Control and Resveratrol + H₂O₂ groups than H₂O₂ group. Correspondingly, compared to H₂O₂ group, the expressions of Mn-SOD and nuclear Nrf2 (N-Nrf2) was up-regulated in Resveratrol + H₂O₂ group (p < 0.05). In vivo, compared with the saline group, using resveratrol could significantly accelerate wound healing of rats on Day 14 (p < 0.05) and make the regenerated skin structure more complete and inflammatory response lower. Moreover, the expressions of Mn-SOD was significantly up-regulated after using resveratrol.ConclusionsResveratrol has the positive effects on promoting the acceleration and quality of skin wound healing, which maybe at least in part caused by the up-regulation of nuclear Nrf2 and Mn-SOD that subsequently attenuated oxidative stress.     

超声造影参数成像技术诊断乳腺良恶性结节的价值

目的:探索超声造影参数成像技术诊断乳腺良恶性结节的价值。方法:回顾性分析67例经病理证实的女性乳腺结节的超声造影图像。与病理结果对比,评价参数成像技术诊断乳腺良恶性结节的准确性。比较超声造影参数成像诸参数在乳腺良恶性结节的组间差异,参数包括:定量参数Area、Intensity、AT（arrival time）、AT-SD（arrival time-standard deviation）及定性参数灌注方式、灌注均匀性。并对组间差异有统计学意义的参数进一步利用ROC曲线评估其诊断价值。结果:超声造影参数成像技术诊断乳腺良恶性结节的敏感度、特异度、准确度分别为84.6%、63.4%、71.6%。年龄、定量参数Area及AT在乳腺结节良恶性组间差异有统计学意义（P<0.05）,且对诊断乳腺良恶性结节有一定价值,ROC曲线下面积分别为0.77、0.80、0.82。联合年龄、Area及AT诊断乳腺结节良恶性的价值更高,ROC曲线下面积高达0.92。定量参数Intensity、AT-SD和定性参数灌注方式及灌注均匀性在乳腺结节良恶性组间差异无统计学意义（P>0.05）。结论:超声造影参数成像技术是一种可用于观察乳腺病变血流动力学特点的成像工具,在诊断乳腺良恶性结节方面具有一定价值。     

置管溶栓治疗恶性肿瘤患者急性下肢深静脉血栓的疗效分析

目的:探讨Unifuse导管接触溶栓治疗恶性肿瘤患者急性下肢深静脉血栓形成(deep venous thrombosis,DVT)的临床效果。方法:将我院2014年1月至2016年8月经多普勒超声确诊的16例恶性肿瘤合并急性下肢DVT患者为研究对象,采用下腔静脉滤器置入加置管溶栓,应用尿激酶高压注射器冲击溶栓剂量为30万U,微量泵溶栓（236±42.34）万U,以及球囊扩张等方法,以术前、后的健侧、患肢周径差、静脉通畅度评价疗效。结果:治疗后,膝上周径减少（8.6±0.9）cm,vs （2.7±0.6）cm,膝下周径减少（3.5±0.3）cm,vs （1.3±0.5）cm。术前、后静脉通畅度评分显著改善（8.3±1.1 vs 2.0±0.6,P<0.05）,治疗过程中无严重并发症。结论:急性下肢深静脉血栓可能是恶性肿瘤病变的一种局部临床表现,要重视其合并恶性肿瘤的可能,减少漏诊率。应用尿激酶经腘静脉置管溶栓治疗急性下肢深静脉血栓形成患者具有见效快、治愈率高等特点,效果显著,症状缓解明显,能够迅速恢复深静脉血流,值得临床推广。     

Is it necessary to perform prehabilitation exercise for patients undergoing total knee arthroplasty: meta-analysis of randomized controlled trials

Objectives: This study was designed to test whether it is necessary to perform prehabilitation exercise for patients undergoing total knee arthroplasty (TKA).

Methods: Literatures were identified from Pubmed, Clinicaltrials, Cochrane library, and SpringerLink. All studies that compared a prehabilitation exercise group with control group before TKA were included. The primary outcome was length of hospital stay. Secondary outcomes were quadriceps strength and functional ability in short term (1.5 to 3 months) after TKA.

Results: There was significant difference in the length of hospital stay, knee range of motion (ROM) and sit-to-stand test (P＜0.05). No statistical differences were found in quadriceps strength, 6-minute walk, ROM, knee extension, knee flexion, WOMAC pain, WOMAC function, WOMAC stiffness between the two groups in short term after TKA (P＞0.05).

Conclusions: Our meta-analysis found that prehabilitation exercise was effective in reducing length of hospital stay. Importantly, it was an effective method for improving knee ROM and sit-to-stand test after TKA. However, there was no effect of prehabilitation exercise on the improvement of quadriceps strength, 6-minute walk, pain and functional recovery following TKA.     

傣药麻三端的育亨宾植物资源研究

“麻三端”（云南萝芙木）Rauvolfia yunnanensis Tsiang为云南傣族的药用植物种类，本文对其植物资源进行了初步研究并测定出其育亨宾成分含量高于育亨宾的药源之一萝芙木的含量。结果表明：“麻三端”不仅较广泛分布于我国南部并在分布区域内也常见，且育亨宾的含量较高，是较为理想的育亨宾药用植物资源。     

微量元素硒对实验性动脉粥样硬化消退的影响

本研究观察了亚硒酸钠对高胆固醇饮食造成鹌鹑动脉粥样硬化消退的影响。结果表明,硒使动物血清总胆固醇,甘油三酯和脂质过氧化物持续显著降低,并明显提高高密度脂蛋白胆固醇/总胆固醇比值。实验15周末动物动脉组织胆固醇含量降低,动脉粥样硬化指数显著减少。提示,硒有明显加速实验性动脉粥样硬化症消退的作用。     

Effect of Interleukin-1β on the Elevation of Cytoplasmic Free Calcium of the Cultured Hippocampal Neurons Induced by L-Glutamate

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Inhibitory effects of ketamine and L--NAME on expression of c-fos and NOS in hypothalamic paraventricular nucleus after acute hypothermia and hypoxia in rats

Thehypothalamusisaverycomplexbrainregionencompassingnucleiwithdifferentrolesinregulationofendocrine,autonomicandbehavioralactivities.Hypothermiaandhypoxiaisacomplicatedsystematichypoxicenvironment.AcutemoderatehypoxiacaninducestrongneurosecretoryreactioninthehypothalamusincludingCRFmRNAexpressionintheparaventricularnucleus(PVN)andincreaseofthelevelofACTHandcortisolinplasma['j.Ithasbeenreportedthathypoxiacaninducethereleaseoflargeamountofaminoacids,especiallyglutamateandasparate,inthebrain…