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511.
Isolation and characterization of a highly enriched preparation of receptosomes (endosomes) from a human cell line. 总被引:18,自引:4,他引:18 下载免费PDF全文
R B Dickson L Beguinot J A Hanover N D Richert M C Willingham I Pastan 《Proceedings of the National Academy of Sciences of the United States of America》1983,80(17):5335-5339
Receptor-mediated endocytosis proceeds by transfer of receptor-ligand complexes from clathrin-coated pits at the cell surface to uncoated endocytic vesicles termed receptosomes (or endosomes). These vesicles have now been purified more than 37-fold based on their content of newly internalized epidermal growth factor. 125I-labeled EGF was bound to human KB carcinoma cells at 4 degrees C, and then the cells were warmed to 37 degrees C for 8 min and disrupted. The purification scheme involved density gradient centrifugation on colloidal silica and sucrose and gel filtration on Sephacryl S-1000. Relative to homogenate, receptosomes are enriched 4.3-fold in their cholesterol content and depleted in enzyme markers for plasma membranes (2- to 3-fold) and lysosomes (9-fold). Receptosomes have a polypeptide composition that is different from plasma membrane, lysosome, and other homogenate fractions. They are enriched in transferrin receptors (30-fold) and in unidentified Mr 70,000-75,000 glycoprotein(s); they contain phosphomannosyl receptors. They do not contain detectable amounts of clathrin. 相似文献
512.
Nanoparticle research is currently an area of intense scientific interest due to a wide variety of potential applications. Human beings have been exposed to airborne nanosized particles throughout their evolutionary stages, and such exposures have increased dramatically over the last century. Nanoparticle can modify the physicochemical properties of the material as well as create the opportunity for increased uptake and interaction with biological tissues through inhalation, ingestion, and injection. This combination of effects can generate adverse biological effects in living cells. Nanoparticles have proved toxic to human once in the blood stream, nanoparticles, spleen, bone marrow and nervous system can be transported around the body and be taken up by organs tissue and cell cultures, resulting in increased oxidative stress, inflammatory cytokine production and cell death. Unlike larger particles, nanoparticles may be taken up by cell mitochondria and the cell nucleus studies demonstrate the potential for nanoparticles to cause DNA mutation and induce major structural damage to mitochondria, even resulting in cell death. Size is therefore a key factor in determining the potential toxicity of a particle. How these nanoparticles behave inside the body is still a major question that needs to be resolved. There is a responsibility to test and optimize these new nanomaterials early during the development process to eliminate or ameliorate identified toxic characteristics. 相似文献
513.
To understand how receptors are involved in neuronal trafficking and to be able to utilize them for specific targeting via the peripheral route would be of great benefit. Here, we describe the generation of novel lentiviral vectors with tropism to motor neurons that were made by coexpressing onto the lentiviral surface a fusogenic glycoprotein (mutated sindbis G) and an antibody against a cell-surface receptor (Thy1.1, p75NTR, or coxsackievirus and adenovirus receptor) on the presynaptic terminal of the neuromuscular junction. These vectors exhibit binding specificity and efficient transduction of receptor positive cell lines and primary motor neurons in vitro. Targeting of each of these receptors conferred to these vectors the capability of being transported retrogradely from the axonal tip, leading to transduction of motor neurons in vitro in compartmented microfluidic cultures. In vivo delivery of coxsackievirus and adenovirus receptor-targeted vectors in leg muscles of mice resulted in predicted patterns of motor neuron labeling in lumbar spinal cord. This opens up the clinical potential of these vectors for minimally invasive administration of central nervous system-targeted therapeutics in motor neuron diseases. 相似文献
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Dr. Curt Hagen Dr. Schnoor Dr. Richert Dr. Peschel Dr. M. Raede Dr. Zimmer 《Langenbeck's archives of surgery / Deutsche Gesellschaft fur Chirurgie》1894,39(5-6):577-584
Ohne Zusammenfassung 相似文献
516.
Danielle Glista PhD Robin O'Hagan BA Danielle DiFabio BHSc Sheila Moodie PhD Karen Muñoz EdD Ioan Aurelian Curca PhD Christine Meston PhD Frances Richert MSc Dave Pfingstgraef MClSc Luxshmi Nageswaran BHSc student Christine Brown MClSc Keiran Joseph MSc Marlene Bagatto PhD 《Journal of evaluation in clinical practice》2023,29(4):614-621
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Yvonne Nong MS Jessica Maloh ND Nicole Natarelli BA Hemali B. Gunt PhD Esther Tristani PhD Raja K. Sivamani MD MS AP 《Journal of Cosmetic Dermatology》2023,22(8):2166-2173
Background
Beeswax is a naturally occurring product secreted from worker bees that has varied uses in modern day. In skincare, its function ranges from its role as an occlusive, helping to create a semi-occlusive skin barrier that minimizes transepidermal water loss; as a humectant, locking in hydration; and an emollient to soften and soothe the skin. As a natural substance, its use has been shown to help alleviate symptoms associated with common cutaneous conditions like dermatitis, psoriasis, and overgrowth of normal skin flora.Aims
In this narrative review, we aim to describe current uses of beeswax in skincare that has been published in the literature.Materials and methods
A review of beeswax related publications was performed by searching the PubMed database studies.Results
A total of five clinical studies were included with three studies on animals and two studies in humans.Discussion
Several studies show the benefits of topical beeswax in supporting the skin barrier.Conclusion
Beeswax can be a low-cost, natural ingredient for use in products. Further studies with topical beeswax are warranted. 相似文献519.
Matilde Iorizzo MD PhD Nilton Gioia Di Chiacchio MD PhD Nilton Di Chiacchio MD PhD Chander Grover MD Shari R. Lipner MD PhD Bertrand Richert MD PhD Bianca Maria Piraccini MD PhD Michela Starace MD PhD Antonella Tosti MD 《Pediatric dermatology》2023,40(4):759-761
Intralesional injections of triamcinolone acetonide are widely used to successfully treat several inflammatory nail conditions. This procedure is well described in adults, but less frequently reported in children and teenagers, being largely considered too invasive and fear-provoking for pediatric patients. Our report shows how this procedure is feasible and successful in children, even without a digital block. The step-by-step technique and tips to reduce pain should encourage clinicians to offer it as an alternative option to children with inflammatory nail disorders. 相似文献