全文获取类型
收费全文 | 7863篇 |
免费 | 504篇 |
国内免费 | 43篇 |
专业分类
耳鼻咽喉 | 46篇 |
儿科学 | 122篇 |
妇产科学 | 128篇 |
基础医学 | 1169篇 |
口腔科学 | 286篇 |
临床医学 | 716篇 |
内科学 | 1772篇 |
皮肤病学 | 379篇 |
神经病学 | 951篇 |
特种医学 | 410篇 |
外科学 | 998篇 |
综合类 | 35篇 |
预防医学 | 304篇 |
眼科学 | 126篇 |
药学 | 481篇 |
中国医学 | 2篇 |
肿瘤学 | 485篇 |
出版年
2023年 | 37篇 |
2022年 | 69篇 |
2021年 | 183篇 |
2020年 | 143篇 |
2019年 | 140篇 |
2018年 | 153篇 |
2017年 | 133篇 |
2016年 | 205篇 |
2015年 | 224篇 |
2014年 | 283篇 |
2013年 | 358篇 |
2012年 | 634篇 |
2011年 | 605篇 |
2010年 | 382篇 |
2009年 | 350篇 |
2008年 | 610篇 |
2007年 | 606篇 |
2006年 | 583篇 |
2005年 | 581篇 |
2004年 | 482篇 |
2003年 | 439篇 |
2002年 | 439篇 |
2001年 | 103篇 |
2000年 | 53篇 |
1999年 | 94篇 |
1998年 | 99篇 |
1997年 | 77篇 |
1996年 | 42篇 |
1995年 | 43篇 |
1994年 | 29篇 |
1993年 | 33篇 |
1992年 | 20篇 |
1991年 | 25篇 |
1990年 | 22篇 |
1989年 | 12篇 |
1988年 | 14篇 |
1987年 | 8篇 |
1986年 | 9篇 |
1985年 | 6篇 |
1984年 | 10篇 |
1982年 | 6篇 |
1981年 | 5篇 |
1980年 | 9篇 |
1979年 | 3篇 |
1977年 | 4篇 |
1976年 | 3篇 |
1973年 | 5篇 |
1971年 | 3篇 |
1969年 | 6篇 |
1968年 | 3篇 |
排序方式: 共有8410条查询结果,搜索用时 62 毫秒
51.
Sandra?Siegert Sonja?Thaler Ralf?Wagner Barbara?S?SchnierleEmail author 《AIDS research and therapy》2005,2(1):7
Background
Murine leukemia virus (MLV) vector particles can be pseudotyped with a truncated variant of the human immunodeficiency virus type 1 (HIV-1) envelope protein (Env) and selectively target gene transfer to human cells expressing both CD4 and an appropriate co-receptor. Vector transduction mimics the HIV-1 entry process and is therefore a safe tool to study HIV-1 entry. 相似文献52.
Decrease and gain of gene expression are equally discriminatory markers for prostate carcinoma: a gene expression analysis on total and microdissected prostate tissue 总被引:8,自引:0,他引:8
下载免费PDF全文
![点击此处可从《The American journal of pathology》网站下载免费的PDF全文](/ch/ext_images/free.gif)
53.
54.
Ralf Hanselmann Walther Burchard Rolf Lemmes Dieter Schwengers 《Macromolecular chemistry and physics.》1995,196(7):2259-2275
Ten DEAE (2-(diethylamino)ethyl) dextran samples were investigated by means of static and dynamic light scattering, viscometry and size-exclusion chromatography (SEC) in combination with on-line small-angle laser light scattering (LALLS) and viscometry (VISC). In dilute solution the behavior of DEAE-dextran was compared with that of unsubstituted dextran and the molecular weight M dependences of the radius of gyration Rg, hydrodynamic radius Rh, intrinsic viscosity [η], second virial coefficient A2 and z-average diffusion coefficient D z were determined. The relationships for DEAE-dextran dissolved in a 0,8 molar sodium nitrate solution were nearly the same as for dextran dissolved in water with 0,05 wt.-% sodium azide and gave the same exponents. The molecular weight dependence of the intrinsic viscosity cannot be described by a Kuhn-Mark-Houwink relationship with a constant exponent. The slope in the plot of log [η] versus log M decreases with increasing molecular weight which indicates the occurrence of branching. By means of SEC/LALLS/VISC measurements the molecular weight distributions were determined. The distributions were calculated (1) directly from the light scattering signal, (2) from a calibration line obtained by light scattering data of a DEAE-dextran sample with a broad distribution and (3) from the intrinsic viscosity distribution obtained by the on-line viscosity/refractive index detector in combination with the [η]-M relationship. In order to get the correct molecular-weight dependence of the intrinsic viscosity it is necessary to determine the molecular weight distribution directly by LALLS (technique 1) and to combine this with the appropriate intrinsic viscosity data from the viscometer. Only the third technique, which is an extension of technique 1, gave satisfactory results over the whole molecular weight region observed. 相似文献
55.
Rosenquist R Menestrina F Lestani M Küppers R Hansmann ML Bräuninger A 《Laboratory investigation; a journal of technical methods and pathology》2004,84(2):253-262
Composite lymphomas are rare combinations of Hodgkin's lymphoma (HL) and non-Hodgkin's lymphoma in the same patient, where clonal relatedness has been observed in most of the few cases analyzed. Here, we report a composite classical HL and diffuse large B-cell lymphoma (DLBCL) with interesting molecular features. Micromanipulation of single cells and analysis of V gene rearrangements revealed clonal relatedness with shared and distinct mutations, indicative of derivation from a common germinal center (GC) B-cell precursor and also of further development of both lymphomas in a GC. In the DLBCL, a very high mutation load, including inactivating mutations, and two copies of the same clonal rearrangement with different mutations in single cells were observed. Intriguingly, in the DLBCL precursor somatic hypermutation activity continued after acquisition of destructive V gene mutations, a feature previously found only in Epstein-Barr virus (EBV) infected B-cell expansions. Furthermore, we found evidence of light-chain receptor revision in the lymphoma precursor during a GC reaction. Re-expression of the V(D)J recombination machinery may enhance genomic instability in GC B cells and contribute to lymphomagenesis. 相似文献
56.
We analyzed the effect of the PAF receptor antagonist (+)-cis-3,5-dimethyl-2-(3-pyridyl) thiazolidin-4-one hydrochloride (SM-12502) on the release of leukotriene B4 and IL-8 from human leukocytes. Peripheral blood from healthy donors was separated in two different fractions: polymorphonuclear leukocytes (PMN) and a lymphocyte, monocyte and basophil granulocyte cell fraction (LMB). After incubation of the cell population with different concentrations of SM-12502 the cells were subsequently stimulated with either the Ca ionophore A23187, the bacterial derived peptide fMLP, or with an activator of heterotrimetric G-proteins, the sodium fluoride (NaF, in the presence of Al3+). The PAF receptor antagonist led to a concentration and time dependent inhibition of LTB4 formation and IL-8 release from PMN and LMB. Our data clearly indicate an inhibitory effect of the PAF receptor antagonist SM-12502 on the formation of mediators of the lipoxygenase pathway and on the release of IL-8. 相似文献
57.
Ralf Thomann Chun Wang Jrg Kressler Rolf Mülhaupt 《Macromolecular chemistry and physics.》1996,197(3):1085-1091
The formation of holes during the late stage of the isothermal crystallization in thin films of isotactic poly(propylene) between two cover glasses was observed by light microscopy and atomic force microscopy. This behavior can be described consistently by the well-known negative pressure effect. Light microscopy reveals the simultaneous and sudden occurrence of a large number of small holes at the liquid-solid interface after the liquid in front of the spherulites is completely confined by other spherulites for a certain time interval. In exceptional cases only a few holes appear and finally large cavities are formed. Atomic force microscopy measurements carried out in the height mode are able to prove the hole formation in front of the spherulites. Furthermore, a substantial thinning of the two-dimensional spherulites in thin films can be observed prior to the hole formation. 相似文献
58.
Henn W Niedermayer I Ketter R Reichardt S Freiler A Zang KD 《Cancer Genetics and Cytogenetics》2003,144(1):65-68
We present karyotypes of 15 meningiomas with structural aberrations of chromosome 7, which were taken from a consecutive series of 400 cytogenetically characterized meningiomas. Twelve of these tumors (80%) displayed partial or complete monosomy 7p with a consensus deleted region of 7p12 approximately pter, in 6 of 15 cases arising from an unbalanced whole-arm t(1;7)(q11;p11), and in 4 of 15 cases from a whole-arm translocation involving other chromosomes. Other types of partial aneusomy 7 (3/15 cases) or balanced aberrations of chromosome 7 (2/15 cases) were relatively rare. In most cases (11/15), the centromeric region of chromosome 7 was involved in the rearrangements. We conclude that in meningiomas, the near-centromeric region of chromosome 7 is particularly prone to structural rearrangements most frequently resulting in monosomy 7p. The investigation of the histopathologic features of this rare cytogenetic subgroup of meningiomas showed no clear genotype/phenotype correlation. As 7 of 11 of the meningiomas with monosomy 7p belonged to World Health Organization grades II or III, which usually comprise less than 20% of all meningiomas, partial loss of 7p appears to be involved in tumor progression in meningiomas. Because monosomy 7p is typically associated with the strongly progression-associated monosomy 1p, however, monosomy 7p represents a cofactor more than a stand-alone feature of meningioma progression. 相似文献
59.
Priming of Immunological Memory by Pneumococcal Conjugate Vaccine in Children Unresponsive to 23-Valent Polysaccharide Pneumococcal Vaccine
下载免费PDF全文
![点击此处可从《Clinical and Vaccine Immunology : CVI》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Markus A. Rose Ralf Schubert Nicola Strnad Stefan Zielen 《Clinical and Vaccine Immunology : CVI》2005,12(10):1216-1222
Pneumococcal polysaccharide vaccine (PPV) is of limited immunogenicity in infants and immunocompromised patients. Our prospective randomized controlled trial investigated whether priming with pneumococcal conjugate vaccine (PCV) induced specific immunological memory in previously nonresponders to PPV. Of a total of 33 children (2 to 18 years) with polysaccharide-specific immunodeficiency (PSI), group A (n = 16) received two doses of 7-valent PCV in a 4- to 6-week interval, and a booster dose of 23-valent PPV after one year. Group B (n = 17) received two doses of PPV in a 1-year interval exclusively. Specific antibody concentrations for serotypes 4, 5, 6B, 9V, 14, 18C, 19F, and 23F were determined (enzyme-linked immunosorbent assay) before and at 7 and 28 days after administration of the PPV booster and compared to an opsonophagocytosis assay. Of group A, 64 to 100% had antibody concentrations of ≥1 μg/ml on day 28 after the booster versus 25 to 94% of group B. Group A had significantly higher antibody concentrations for all PCV-containing serotypes already on day 7, indicating early memory response. Antibody concentrations were in accordance with functional opsonic activity, although opsonic titers varied among individuals. Pneumococcal vaccination was well tolerated. The incidence of airway infections was reduced after priming with PCV (10/year for group A versus 15/year for group B). Following a PPV booster, even patients primarily not responding to PPV showed a rapid and more pronounced memory response after priming with PCV. 相似文献
60.