湿润烧伤膏在肛周脓肿术后创面治疗中的应用效果分析

目的观察分析湿润烧伤膏在肛周脓肿术后创面治疗中的应用效果。方法选取2018年2月至2019年7月成飞医院普外科收治的120例肛周脓肿患者作为研究对象,并根据术后干预方法将其分为试验组(60例)与对照组(60例),试验组患者于肛周脓肿根治术后行湿润烧伤膏换药治疗,对照组患者于肛周脓肿根治术后行凡士林油纱换药治疗,对比两组患者术后创面疼痛情况、住院时间及创面愈合时间。结果试验组患者术后第1、2、3天创面视觉模拟评分法(visual analogue scale,VAS)评分均明显低于对照组(t=4.763、5.310、13.730,P均=0.000),住院时间、创面愈合时间均明显短于对照组(t=12.220、5.466,P均=0.000)。结论湿润烧伤膏可有效减轻肛周脓肿术后创面疼痛,促进创面愈合,缩短创面愈合时间,值得临床推广应用。     

The influence of orally administered docosahexaenoic acid on monoamine neurotransmitter,nerve growth factor,and brain-derived neurotrophic factor in aged mice

In this study we attempt to investigate the new role of docosahexaenoic acid (DHA) supplementation on cognitive ability in aged mice. Kunming-line mice were treated with DHA (200, 400?mg/kg body weight/day) for 30 successive days. The cognitive ability of mice was assessed by learning and memory behavioral test; the levels of DHA were assessed by capillary gas chromatography; the levels of dopamine (DA), norepinephrine (NE), and 5-hydroxytryptamine (5-HT) were assessed by high-performance liquid chromatography; the levels of brain-derived neurotrophic factor (BDNF) and nerve growth factor (NGF) proteins were assessed by the enzyme-linked immunosorbent assay method. The results showed the cognitive ability of mice were significantly different between the DHA-treated groups and the aged group, and orally administered DHA can increase the levels of DA, NE and 5-HT, the content of BDNF and NGF proteins in hippocampus, frontal cortex and striatum tissues. In addition, aged-related changes in phospholipid DHA content were seen. Decreases in DHA were mostly corrected by DHA supplementation. These novel data suggest that DHA supplementation can improve the cognitive dysfunction due to aging by increasing the levels of BDNF and NGF protein and the levels of DA, NE, 5-HT to some extent. We speculate that the mechanism of DHA on cognitive ability may have a beneficial effect on signaling networks through modulation of monoamine neurotransmitters and neurotrophic factors in brain aging.     

血浆置换联合化疗治疗重症儿童噬血细胞综合征的护理

目的总结血浆置换联合化疗治疗重症儿童噬血细胞综合征的临床疗效及护理。方法回顾性总结我科2007年11月-2012年11月间收治的48例重症噬血细胞综合征患儿,采用单纯化疗（对照组）18例,血浆置换联合化疗（实验组）30例,观察临床疗效及总结护理方法。结果对照组8例病情好转转血液科继续治疗,10例死亡,存活率43．4％。实验组病例23例病人病情好转,转血液科继续治疗;7例死亡,存活率76．7％。试验组存活率明显高于对照组的单纯化疗,差异有统计学意义（P〈0．05）。结论血浆置换联合化疗治疗能提高重症儿童噬血细胞综合征的临床疗效,把握好血浆置换治疗的各个环节,熟悉化疗药物的作用及不良反应,有针对性地做好药物护理,同时做好家属的思想工作,使家属配合治疗,可提高惠儿的成活率。     

消瘀定痛散治疗中重度膝关节骨关节炎临床研究

目的评价消瘀定痛散治疗中重度膝关节骨关节炎的临床疗效。方法将符合条件的中重度膝关节骨关节炎患者72例随机分为两组,治疗组给予消瘀定痛散外敷治疗。对照组给予扶他林乳胶剂外涂治疗．治疗前和治疗结束时分别以视觉模拟疼痛评分（VAS）和WOMAC功能评分进行临床评价。结果VAS评分：治疗组治疗前后分别为（6．8±2．2）和（2．1±1．2）,对照组治疗前后分别为（6．7±2．4）和（4．8±1．5）。WOMAC功能评分：治疗组治疗前后分别为（63．8±10．2）和（22．3±11．6）,对照组分别为（62．7±11．6）和（40．8±12．1）,两组比较有统计学意义（P〈0．05）,治疗组优于对照组。结论消瘀定痛散可以缓解中重度骨关节炎引起的疼痛,改善膝关节功能,值得推广应用。     

八角莲的TLC鉴别和HPLC指纹图谱研究

目的建立八角莲TLC鉴别方法和HPLC指纹图谱,为其质量控制提供专属性更好的测试方法。方法采用乙酸乙酯超声提取制备供试品溶液,以氯仿-甲醇-水（9∶1∶0.1）作为展开系统,展距13cm,10%硫酸乙醇溶液为显色剂;采用HPLC法,Thermo AcclaimTM 120 C18（4.6×250 mm,5 μm）色谱柱,乙腈-0.05%磷酸水溶液为流动相,梯度洗脱,流速为1.0mL/min,检测波长为203nm,柱温36℃;采用中药色谱指纹图谱评价系统进行相似度分析。结果建立了八角莲药材的TLC鉴别方法和HPLC指纹图谱,确定11个共有色谱峰,方法学验证结果符合指纹图谱相关要求。结论该方法简单、可靠,可用于八角莲药材的鉴别和质量控制。     

Development of peptidic dopamine transporter inhibitors via aromatic modification-mediated conformational restriction

The dopamine transporter plays an important role in the molecular mechanism of cocaine dependence. It is suggested that inhibitors of the dopamine transporter would have strong therapeutic potential. Here we report that aromatic modification can constrain a linear peptide into the beta-turn conformation, which is preferred by the dopamine transporter. On the basis of this finding, a novel selective and competitive peptidic inhibitor of the dopamine transporter was developed. The peptide binds to the dopamine- and cocaine-binding site of the dopamine transporter and has behavioral effects different from those of cocaine in mice.     

High-performance liquid chromatographic method for the bioequivalence evaluation of desloratadine fumarate tablets in dogs

A simple HPLC method was developed for the determination of desloratadine in dog plasma and was used for evaluating the bioequivalence of desloratadine fumarate tablets and desloratadine tablets in dogs. Chromatographic separation was performed on a Hypersil CN column (150 mm×5.0 mm, 5 μm) using a mixture of methanol, acetonitrile and phosphate buffer (pH 5.5; 0.01 mol/l) (35:35:30, v/v/v) as mobile phase delivered at a flow rate of 0.8 ml/min. The detection was set at 241 nm. The limit of quantitation was 5.0 ng/ml. The calibration range was from 5.0 to 800.0 ng/ml. Inter- and intra-day precision ranged from 1.8 to 3.8% and from 2.2 to 9.0%, respectively. The recovery of desloratadine from dog plasma ranged from 78.8 to 82.0%. The developed method was applied to the bioequivalence studies of desloratadine fumarate tablets (test preparation) and desloratadine tablets (reference preparation) in five dogs. Pharmacokinetic parameters t_max, C_max, AUC_0–t, AUC_0–∞, t_1/2 were determined from plasma concentration-time profiles of both preparations. The analysis of variance (ANOVA) did not show any significant difference between the two preparations and 90% confidence intervals fell within the acceptable range for bioequivalence. Based on these statistical inferences it was concluded that the two preparations exhibited comparable pharmacokinetic profiles and that desloratadine fumarate tablets was bioequivalent to desloratadine tablets.     

Determination of ibuprofen in dog plasma by liquid chromatography and application in pharmacokinetic studies of an ibuprofen prodrug in dogs

A liquid chromatography (LC) method for the determination of ibuprofen in dog plasma is described. Chromatographic separation was performed on a Diamonsil C18 column with a C18 guard column using a binary mixture of acetonitrile and 0.02 mol/l phosphate buffer (pH 6.5) (35:65, v/v) delivered at a flow rate of 1.2 ml/min. The linear range for ibuprofen was from 1.0 to 40.0 microg/ml with a limit of quantitation of 1.0 microg/ml. Within-run accuracy and precision ranged from -0.1% to 4.0% and from 1.1% to 5.5% and between-run accuracy and precision ranged from -1.1% to 4.7% and from 1.3% to 7.0%, respectively. The mean extraction recoveries of ibuprofen determined over the concentrations of 1.0, 10.0, and 40.0 microg/ml were (100.5+/-1.8)%, (99.8+/-1.0)%, and (99.2+/-2.3)%. The developed LC method greatly simplified the sample preparation and adopted mild conditions to prevent the possible hydrolysis of the prodrug and was successfully applied to the pharmacokinetic studies of an ibuprofen prodrug in dogs.     

富锗灵芝的临床与实验研究

为探讨富锗灵芝的抗衰老作用，取普通灵芝用雄性ＩＣＲ小鼠８０只作对照实验。两种灵芝均能明显增加小鼠体重，延长游泳时间和增强小鼠记忆力，但二者之间无显著差异。临床用于老年虚证患者４８例，试验组服富锗灵芝胶囊，３０例，对照组服普通灵芝胶囊，１８例。结果试验组有效率达９６．７％，并在改善乏力、心悸、腰酸等方面均明显优于普通灵芝组，ＣＤ３、ＣＤ４、自然杀伤细胞（ＮＫ细胞）、超氧化物歧化酶（ＳＯＤ－１）亦明显     

网络环境中医院图书馆的导读工作与文献资源建设

网络环境对医院医务人员图书馆的导读工作提出了新的挑战，面对新的环境、新的问题，导读工作应该采取相应的对策和策略，并建设相应的文献资源。