Cd1d-restricted cellular lysis by peripheral blood lymphocytes: relevance to the inflammatory bowel diseases

The CD1d molecule has been implicated to play a role in inflammatory bowel diseases (IBD), possibly through its presentation of an intestinal antigen trigger. To understand the role of the CD1d class I-like protein in IBD, we investigated the molecule's expression in diseased intestinal tissue and determined its potential to undergo specific recognition by intraepithelial and peripheral blood lymphocytes (PBLs) derived from IBD patients. We have observed an increase in precipitable CD1d in inflamed tissues, which suggests CD1d up-regulation in IBD; this was not accompanied by the occurrence of CD1d-specific cytotoxicity by lymphocytes isolated from the same tissue sites. In contrast, we have observed CD1d-specific cytotoxicity by PBLs from both patients and normal controls mediated by a possibly unique type of lymphocytic cell. These observations support a model in which intestinal inflammation may be initiated by circulating PBLs following the tissue-specific upregulation of CD1d. These activated PBLs may then be the source of the extraintestinal manifestations observed with IBD. We therefore propose that the cells responsible for this activity may play a role in regulating immune responses through the specific recognition of CD1d-specific antigen(s).     

充血性心力衰竭患儿血清白细胞介素-18和淋巴细胞CD54变化

目的探讨IL-18和CDs4在小儿充血性心力衰竭（CFIF）发病中的作用，及其对z],JLCFIF的诊断价值。方法CFIF患儿52例，心功能按修订的Ross和Reithman评分系统。心功能Ⅱ级（3～6分）18例、Ⅲ级（7～9分）17例，Ⅳ级（10～12分）17例。原发疾病为扩张性心肌病、心内膜弹力纤维增生症及先天性心脏病等。对照组15例为上呼吸道感染患儿。血清IL-18水平用ELISA法进行测定，采用流式细胞术检测外周血淋巴细胞CDs4表达。结果CFIF患儿血清IL-18及CDs4均显著增高（P均〈0．001），且随CFIF加重渐增加，治疗后两者均较治疗前明显降低（P均〈0．05）；且两者在心肌病组、先天性心脏病组及其他病组3组间比较差异无显著性（P均〉0．05），但均显著高于对照组（P均〈0．01）。结论IL-18和CDs4可能参与小儿CFIF发生发展。可以作为评价CHF严重程度的生物化学标记；CFIF作为一种临床综合征，无论最初病因是否相同，免疫反应可能都具有某些共同特征。     

青少年膳食钙摄入状况对骨密度的影响

目的了解青少年膳食钙摄入状况及骨量水平。方法对昆明市98名12～14岁青少年问卷调查了解膳食营养状况,特别是每日摄入牛奶量的情况,使用双能X线骨密度测定仪(DEXA)测量腰椎及髋部骨密度(BMD)。结果每日坚持喝牛奶的学生骨密度显著高于不喝牛奶的学生。结论膳食中牛奶是影响青少年骨量水平的主要因素。     

血浆游离双链DNA在原发性肝癌中的诊断价值

目的:探讨血浆中游离双链DNA(cell-free double-stranded DNA,cf-dsDNA)在原发性肝癌(primary hepa-tic carcinomas,PHC)诊断中的应用.方法:招募62例PHC患者和41例良性肝病患者以及45例健康对照,填写信息登记表.抽取治疗前患者和健康对照的空腹血样,...     

吉非替尼治疗晚期非小细胞肺癌疗效分析

目的观察吉非替尼对晚期非小细胞肺癌患者的疗效。方法对吉非替尼治疗的非小细胞肺癌22例进行分析。结果22例中可评估的14例中(5例疾病稳定,2例完全缓解,1例部分缓解)有效率21.4%,临床获益率8/14(57.2%)。结论吉非替尼对晚期非小细胞肺癌疗效较好,其副反应相对小,耐受性好。     

推拿掌振法对局部皮肤温度场红外热像的影响

目的:通过红外热像技术,分析确定振法的热效应及操作时间的客观化标准。方法:利用红外热像技术采集初学者、低年资医师及高年资医师三组操作者采用的空置、掌振及空振法所得的相应红外热像数据,比较不同组别的温度差异。结果:三组操作者在空置后3min,心俞穴和大杼穴处温度均出现变化,且与操作前温度有显著差别(P<0.05);在空置3min后,被操作者的心俞处以及大杼处的温度呈自然下降趋势;而在行掌振法时,被操作者心俞处以及大杼处的温度变化,不仅发生在3min后,而且可延续到振法停止后的1min到3min内,与操作前温度相比,有显著差别(P<0.05);在空振中,高年资医师的劳宫处和中冲处温度呈现逐渐上升趋势,在振法进行到90 s时,中冲处温度出现显著变化,与开始温度相比,有显著差别(P<0.05),而劳宫处的温度则是在120 s时,出现显著变化,与开始温度相比,有显著差别(P<0.05)。结论:推拿手法有一定的热传导作用,但振法产热不仅仅是热传导作用,还有可能是激发了受试者体内的某种产热机制(其机制有待进一步研究);从空振试验模块推拿医师手部的升温现象分析,振法的持续操作时间至少应该达到90 s,最佳操作时间可能在3min以上。     

Comparison of in vivo and in vitro percutaneous absorption of T-2 toxin in guinea pigs

The fate and distribution of T-2 were examined in 6 guinea pigs. T-2 (1.2 micrograms/cm2), in methanol or DMSO, was painted onto the shaved backs of guinea pigs, a screen barrier was applied, urine and feces were collected daily and the guinea pigs were killed after 48 hr. Disks of skin (lateral to the in vivo site of application) were excised from the guinea pigs and used for in vitro penetration studies with static diffusion cells. Skin excised from 6 additional guinea pigs was used for penetration studies with flow-through diffusion cells. For in vitro studies, T-2 dissolved in methanol or DMSO was applied to the epidermal surfaces and the appearance of penetrant in receptor fluid bathing the dermal surfaces was monitored for 48 hr. Metabolism of T-2 was measured by using thin layer radiochromatography to identify metabolites. In the in vivo study, mean cutaneous absorption (n = 3) after 48 hr (expressed as per cent dose) was 22.5 and 51.9 for the methanol and DMSO groups, respectively. In vitro cutaneous penetration for static diffusion cells was 3.9 and 38.4 for the methanol and DMSO groups. For flow-through diffusion cells, mean penetration (n = 9) was 14.6 and 42.6 for the methanol and DMSO groups. Urinary metabolites of T-2 were T-2 triol, 3' OH-HT-2, T-2 tetraol, the glucuronide conjugate of HT-2 and several more polar metabolites. The main metabolite of T-2 in the receptor fluid bathing the dermal surfaces of excised skin was HT-2.     

大黄清胃冲剂活性成分提取工艺的均匀设计优化选择

目的 探讨大黄清胃冲剂活性成分提取分离的优化工艺条件。方法 以游离蒽醌提取率为定量指标,通过４种生长黄煎煮方法的初步筛选,确定浸渍溶剂、浸渍时间、回流溶剂及回流时间为考察因素,并为Ｕ９（９＾６）均匀表设计试验。结果 大黄提取优化工艺条件为７０％乙醇浸润１４ｈ和回流提取８０ｍｉｎ。结论 工艺条件可以用于大黄清胃冲剂的生产制备。