全文获取类型
收费全文 | 11840篇 |
免费 | 486篇 |
国内免费 | 30篇 |
专业分类
耳鼻咽喉 | 353篇 |
儿科学 | 969篇 |
妇产科学 | 405篇 |
基础医学 | 757篇 |
口腔科学 | 650篇 |
临床医学 | 901篇 |
内科学 | 1908篇 |
皮肤病学 | 366篇 |
神经病学 | 842篇 |
特种医学 | 518篇 |
外科学 | 2951篇 |
综合类 | 72篇 |
一般理论 | 6篇 |
预防医学 | 320篇 |
眼科学 | 368篇 |
药学 | 637篇 |
中国医学 | 73篇 |
肿瘤学 | 260篇 |
出版年
2024年 | 13篇 |
2023年 | 146篇 |
2022年 | 63篇 |
2021年 | 166篇 |
2020年 | 138篇 |
2019年 | 157篇 |
2018年 | 318篇 |
2017年 | 268篇 |
2016年 | 316篇 |
2015年 | 250篇 |
2014年 | 367篇 |
2013年 | 555篇 |
2012年 | 721篇 |
2011年 | 932篇 |
2010年 | 477篇 |
2009年 | 250篇 |
2008年 | 662篇 |
2007年 | 819篇 |
2006年 | 816篇 |
2005年 | 928篇 |
2004年 | 825篇 |
2003年 | 767篇 |
2002年 | 738篇 |
2001年 | 372篇 |
2000年 | 453篇 |
1999年 | 268篇 |
1998年 | 102篇 |
1997年 | 77篇 |
1996年 | 62篇 |
1995年 | 40篇 |
1994年 | 32篇 |
1993年 | 21篇 |
1992年 | 29篇 |
1991年 | 19篇 |
1990年 | 15篇 |
1989年 | 10篇 |
1988年 | 12篇 |
1987年 | 19篇 |
1986年 | 13篇 |
1985年 | 12篇 |
1983年 | 7篇 |
1982年 | 8篇 |
1981年 | 9篇 |
1980年 | 8篇 |
1979年 | 10篇 |
1978年 | 13篇 |
1977年 | 7篇 |
1976年 | 13篇 |
1975年 | 5篇 |
1974年 | 8篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
31.
Derbent M Yilmaz Z Baltaci V Saygili A Varan B Tokel K 《American journal of medical genetics. Part A》2003,(2):129-135
This report describes the dysmorphic features and frequency of 22q11.2 deletion (del22q11) in 30 Turkish patients with conotruncal heart defects (CTHDs). Fluorescence in situ hybridization (FISH) analysis revealed deletions in the 22q11.2 region in nine (30%) individuals. The CTHDs in this group were tetralogy of Fallot (four cases), double-outlet right ventricle (DORV) (two cases), transposition of great arteries (two cases), and ventricular septal defect (VSD) associated with other CTHDs (one case). The frequency of del22q11 in the study group was relatively high because many of the patients with dysmorphic findings also had cardiac anomalies involving the pulmonary artery, ductus arteriosus, or the aortic arch and its main branches. Twenty of the 30 patients exhibited several dysmorphic findings. Two of the nine patients with del22q11 exhibited no apparent dysmorphic features other than sacral dimple. Interestingly, one of the patients with del22q11 had a phenotypic appearance similar to that seen in oculo-auriculo-vertebral spectrum (OAVS). This individual had left microtia, atresia of the external meatus, mandibular asymmetry, and peripheral facial nerve paralysis. His mental development was normal and there were no abnormalities on ophthalmological examination. The CTHDs in this patient were situs inversus dextrocardia, DORV, pulmonary stenosis, and VSD. Radiographs of this patient showed platybasia, complete fusion of C2-C3, and posterior fusion of the T1-T2 vertebrae. This particular case indicates that the phenotypic features of del22q11 and OAVS may overlap. 相似文献
32.
Adrian Florin Gal Vasile Rus Sanda Andrei Viorel Miclăuş 《Journal of histotechnology》2020,43(2):97-101
ABSTRACT Early detection of apoptotic cells on histological slides is of major importance for both diagnostic and research areas. In the current study, the aim was to propose a convenient method to stain the mitochondria and establish whether hepatocytes undergoing apoptosis can be identified in tissue sections using the proposed method. Liver tissue from five adult chinchillas was fixed with 10% neutral buffered formalin for Goldner’s trichrome (GT) and Groat’s iron hematoxylin and eosin (HE) stains and with Kolster’s fixative for the Heidenhain’s iron hematoxylin procedure. The HE and GT-stained sections showed the morphological features consistent with apoptosis i.e., homogenous intensely acidophilic cytoplasm, cell shrinkage with an irregular outline, nuclear shrinkage with cloudy karyoplasm, and karyopyknosis in the late stage. Sections stained with Heidenhain’s iron hematoxylin method was used to pinpoint mitochondria and revealed cells which were undergoing the first stages of the apoptosis process i.e., disappearance of mitochondria from the cell, chromatin condensation and margination, paracentral localization of nucleoli, and vacuolated nuclei. In more advanced stages of apoptosis, cells presented significant nuclear and cytoplasmic changes. It was concluded that this is the first report targeting the mitochondria, by performing inexpensive histological staining techniques, in order to assess dead cells in situ. 相似文献
33.
Oztürk G Ertaş FN Akyilmaz E Dinçkaya E Tural H 《Artificial cells, blood substitutes, and immobilization biotechnology》2004,32(4):637-645
A biosensor for specific determination of hydrogen peroxide was developed by using homogenized artichoke (Cynara scolymus L.) tissue in combination with a dissolved oxygen probe and applied in determination of hydrogen peroxide in milk samples. Artichoke tissue, which has catalase activity, was immobilized with gelatine by means of glutaraldehyde and fixed on a pretreated teflon membrane. The electrode response was maximum when 0.05 M phosphate buffer was used at pH 7.0 and at 30 degrees C. Upon addition of hydrogen peroxide, the electrode gives a linear response in a concentration range of 5.0-50 x 10(-5) M with a response time of 3 min. The method was also applied to the determination of hydrogen peroxide in milk samples. 相似文献
34.
Telomere-specific fluorescence in situ hybridization analysis of couples with five or more recurrent miscarriages 总被引:1,自引:0,他引:1
Fluorescence in situ hybridization analysis using telomere specific probes has been used to detect cryptic translocations in the chromosomal telomeric regions. This study was performed in five clinically normal couples who have had five or more spontaneous abortions and whose karyotypes were found to be normal using conventional cytogenetic techniques. Using the telomere specific probes, in one couple we determined a cryptic translocation between chromosome 3 and 10, and, in another couple, the signal in chromosome 20 was detected in another chromosome, which was probably a D group chromosome. Additionally, in the latter and also in two other couples, we observed a polymorphism. The approach will be helpful for screening cryptic translocations using telomere specific multiple probe sets in couples with recurrent miscarriages. As prenatal diagnosis will be available for these couples for future pregnancies, it will be possible to help these families to have healthy fetuses. 相似文献
35.
Gürpinar OA Tuzlakoğlu K Onur MA Tümer A Serdar MA Unal N Pişkin E 《Journal of biomaterials science. Polymer edition》2003,14(6):589-600
In this study, attachment and growth of Baby Hamster Kidney (BHK) cells on ethylene diamine (EDA)-plasma-treated poly(L-lactide/epsilon-caprolactone) biodegradable copolymer films were investigated. The co-polymer (Mw: 58000; Mn: 35000 and PI 1.60) was synthesised by ring-opening polymerization of the respective dimers with using stannous octoate as the catalyst. The final ratio of L-lactide to epsilon-caprolactone obtained by 1H-NMR was 87:13. The co-polymer films were treated with the EDA-plasma in a glow-discharge apparatus. The BHK-30 cell line was cultured on plain and EDA-plasma-treated films and their pre-wetted forms (with ethanol and/or cell culture medium before use). Cell attachment and growth were followed. Alkaline phosphatase (ALP) activity and glucose uptake in cell culture medium were also investigated. There was no attachment in the first 12 h. Glow-discharge treatment increased significantly the attachment and growth. Pre-wetting with ethanol and cell culture medium was also increase significantly both the attachment and growth. 相似文献
36.
Myoepithelial carcinoma is an extremely rare, malignant epithelial tumor which is usually encountered in the parotid region. In this report, a myoepithelial carcinoma arising from a minor salivary gland in the nasopharynx is presented, along with a discussion of the clinical, histopathological and immunocytochemical characteristics of this rare disorder. Larger clinical series and longer follow-up periods are needed in order to establish the best therapy option for these patients. 相似文献
37.
Somatic mosaicism for a MECP2 mutation associated with classic Rett syndrome in a boy 总被引:4,自引:0,他引:4
Topçu M Akyerli C Sayi A Törüner GA Koçoğlu SR Cimbiş M Ozçelik T 《European journal of human genetics : EJHG》2002,10(1):77-81
Rett syndrome is a severe neurodevelopmental disorder that arises from mutations in the X-linked MECP2 gene. It is almost exclusively seen in girls due to the predominant occurrence of the mutations on the paternal X-chromosome, and also the early postnatal lethal effect of the disease causing mutations in hemizygous boys. We identified a boy with features of classic Rett syndrome who is mosaic for the truncating MECP2 mutation R270X. Chromosome analysis showed normal karyotype. These results indicate that a MECP2 mutation associated with Rett syndrome in females could lead to a similar phenotype in males as a result of somatic mosaicism. 相似文献
38.
Poly(D,L-lactide/epsilon-caprolactone)/hydroxyapatite composites as bone filler: an in vivo study in rats 总被引:1,自引:0,他引:1
Senköylü A Ural E Kesencì K Sìmşek A Ruacan S Fambri L Migliaresi C Pìskìn E 《The International journal of artificial organs》2002,25(12):1174-1179
In this study, a novel composite bone substitute was implanted in animal models (rats) and their in vivo characteristics were examined. A D,L-lactide and E-caprolactone copolymer (Mw: 80,000; Mn:40,000, and PI:2.00) was synthesized by ring-opening polymerization of the respective dimers using stannous octoate as the catalyst. The final ratio of D,L-lactide to epsilon-caprolactone obtained by 1NMR was 60/40. Hydroxyapatite (HA) powder was loaded in the copolymer. The HA/copolymer ratio was 60/40 (w/w). These composites were easily shaped by hand. Animal tests were performed on mature wistar rats (n=30). Defects were created on the proximal, the thickest part of the femur. The bone defects of the first group were filled with polymer/HA composite, the second group filled with only HA and the third group was left empty. Histologic examination of bone tissues showed new bone formation around the yellow-green polymer/HA composite material in the first group of animals whereas no evidence of new bone growth was observed in other groups. 相似文献
39.
B. Şahin S. Paydaş E. Coşar K. Biçakçi B. Hazar 《European journal of clinical microbiology & infectious diseases》1996,15(11):866-869
Several problems in the management of life-threatening mucormycosis remain unresolved, necessitating new methods of management. Four patients with histopathologically proven rhinocerebral mucormycosis were treated with high cumulative doses of granulocyte colony-stimulating factor (G-CSF). All had multiple predisposing factors for mucormycosis, particularly leukemia and neutropenia. Two patients refractory to fluconazole therapy were treated with liposomal amphotericin B. The improvement in clinical manifestations was closely related to neutrophil recovery, and all patients were alive at the end of therapy. In addition to surgical debridement and antifungal therapy, G-CSF seems to have played a role in their survival. 相似文献
40.