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51.
Julien Obiols Anne-Sophie Bargnoux Nils Kuster Pierre Fesler Laurence Piéroni Stéphanie Badiou Anne-Marie Dupuy Jean Ribstein Jean-Paul Cristol 《Clinical biochemistry》2013
Objectives
Analytical and clinical performances of the new standardized cystatin C particle-enhanced turbidimetric immunoassay (PETIA) using DiaSys reagents on Olympus AU2700® analyzer were evaluated.Design and methods
We have studied imprecision, linearity, limit of detection and limit of quantification of this new immunoassay. Method comparison was assessed in relation to results generated by the standardized Siemens-particle-enhanced nephelometric immunoassay (PENIA). In order to evaluate the clinical relevance of this assay, estimated glomerular filtration rate (GFR) was calculated using MDRD, CKD-EPI creatinine, CKD-EPI cystatin C 2012 and CKD-EPI creatinine–cystatin C 2012 equations and compared to GFR measured using urinary clearance of 99mTc-DTPA in 100 hypertensive patients.Results
Cystatin C measurements using DiaSys reagents have reliable analytical performances and are comparable to the standardized Siemens-PENIA method (bias of 0.01 mg/L). The mean measured GFR was 90.0 ± 29.7 mL/min/1.73 m2. Bias and accuracy of the three CKD-EPI equations were better than the MDRD. Both CKD-EPI creatinine-based and cystatin C-based formulae had similar bias, precision and accuracy. The combined creatinine–cystatin C equation was significantly more accurate and precise than the CKD-EPI creatinine equation in patients with GFR above 60 mL/min/1.73 m2.Conclusions
The use of cystatin C in a combined equation with creatinine could improve the accuracy of eGFR in the reference interval. 相似文献52.
Antoni Rosell Rosa López-Lisbona Noelia Cubero Carme Obiols Francisco Rivas Jordi Dorca 《Archivos de bronconeumologia》2011,47(7):371-373
The persistent air leaks due to alveolopleural fistula is not an uncommon complication of pneumothorax, particularly secondary ones. Unidirectional flow endobronchial valves initially designed for lung volume reduction are the latest tool in the therapeutic armamentarium.We present a patient with chronic obstructive pulmonary disease (COPD) with a persistent pneumothorax in spite of three pleurodesis, which was able to be resolved with the placement of two IBV? valves. 相似文献
53.
AC Winter K Berger JE Buring & T Kurth 《Cephalalgia : an international journal of headache》2009,29(2):269-278
We evaluated the association of body mass index (BMI) with migraine and migraine specifics in a cross-sectional study of 63 467 women aged ≥ 45 years, of whom 12 613 (19.9%) reported any history of migraine and 9195 had active migraine. Compared with women without migraine and a BMI < 23 kg/m2 , women with a BMI ≥ 35 kg/m2 had adjusted odds ratios (ORs) (95% confidence intervals) of 1.03 (0.95, 1.12) for any history of migraine. Findings were similar for active migraineurs. Women with a BMI of ≥ 35 kg/m2 had increased risk for low and high migraine frequency, with the highest estimate for women who reported daily migraine. Compared with women with the lowest associated risk (migraine frequency < 6 times/year; BMI between 27.0 and 29.9 kg/m2 ), women with a BMI ≥ 35 kg/m2 had an OR of daily migraine of 3.11 (1.12, 8.67). Among the women with active migraine, a BMI ≥ 35 kg/m2 was associated with increased risk of phonophobia and photophobia and decreased risk of a unilateral pain characteristic and migraine aura. Our data confirm previous findings that the association between BMI with migraine is limited to migraine frequency and specific migraine features. 相似文献
54.
In a retrospective study, clinical risk factors of the neonatal period were correlated with the severity of regressed retinopathy of prematurity (ROP) in a population of preterm infants (bw less than 1500 g and or gestational age less than 33 weeks). At the age of 5-11 years 134 out of 528 preterm born infants (25.4%) were found to be under ophthalmic care. Reliable information on eye fundus status could be obtained in 105 of them. Regressed ROP was found in 61, the moderate form in 48 (9.1%) and the severe form in 13 (2.5%) patients. Twelve patients (2.3%) had visual acuity of less than 0.3 on the worst eye and two (0.4%) of these patients were blind from ROP. Twenty-four clinical factors of the newborn period were correlated with the severity of regressed ROP. The results suggest that long oxygen exposure in combination with other factors interfering with retinal vasotonus are associated with the degree of the disease developed. 相似文献
55.
Martínez-Rodríguez J Iranzo A Santamaría J Genís D Molins A Silva Y Meléndez R 《Neurología (Barcelona, Spain)》2002,17(2):113-116
Introduction: Cataplexy is one of the main narcoleptic symptoms and is characterized by sudden loss of muscle tone triggered by emotional stimuli while consciousness is mantained. Clomipramine is an effective treatment of cataplexy. Cataplexy that occurs repeatedly for hours or days is referred to as status cataplecticus.Patients: We report three adults with narcolepsy in whom cataplexy was chronically and effectively treated with clomipramine (75-150 mg/day). For diverse reasons, these three patients had an abrupt withdrawal of clomipramine, and after 2-9 days patients showed an invalidant status cataplecticus characterized by a marked increase of the frequency, duration and severity of their cataplectic attacks that were now elicited by mild emotional stimuli. After introduction of anticataplectic agents (clomipramine in two patients and fluoxetine in one patient), status cataplecticus was resolved in less than a week.Conclusion: In patients with narcolepsy, abrupt withdrawal of chronic treatment with clomipramine may be associated with status cataplecticus. This condition may be resolved with the reintroduction of anticataplectic agents. 相似文献
56.
背景:阿德福韦双酯(ADV) 是一种有效治疗野生型和耐拉米夫定乙肝病毒(HBV)的核苷酸类药物。在使用核苷酸类药物治疗慢性乙肝时,当治疗时间为 48、96、144周时,耐ADV变异体出现的累积发生率分别为0、 0.8-3%和0-5.9%。目的:研究67例对拉米夫定耐药且接受ADV治疗的慢性乙肝患者耐ADV病毒变异体的表型和基因型特点。方法:HBV DNA含量采用实时定量PCR技术。ADV变异体检测采用基质辅助激光解吸电离/飞行时间质谱为基础的基因分型 相似文献
57.
58.
Resistance of Copenhagen rats to chemical induction of glutathione S- transferase 7-7-positive liver foci 总被引:2,自引:0,他引:2
Copenhagen (Cop) rats are completely resistant to the chemical induction of
mammary adenocarcinomas, but their susceptibility to hepatocarcinogenesis
is virtually unknown. Rat liver is a well- characterized and easily
manipulated tissue in which to study carcinogenesis. Therefore, if Cop rats
are resistant to hepatocarcinogenesis, studies into resistance mechanisms
may be feasible. Male Cop and F344 rats, 7-8 weeks old, were initiated
using either N-nitrosodiethylamine (DEN) (200 mg/kg, i.p.) or a two-thirds
partial hepatectomy (PH) followed by N-methyl-N-nitrosourea (MNU) (60
mg/kg, i.p.). The rats were then promoted using a modified resistant
hepatocyte (RH) protocol (a combination of four doses of 2-
acetylaminofluorene (2-AAF) and a single dose of CCl4 that provides a
selective mitotic stimulus for initiated cells). Six weeks after initiation
the rats were killed and liver sections were stained for glutathione
S-transferase 7-7 (GST 7-7), a marker for putative preneoplastic
hepatocytes. Cop rats were found to be highly resistant, having a
approximately 9- and approximately 27-fold smaller percentage of liver area
occupied by GST 7-7-positive foci than susceptible F344 rats following
initiation by DEN and MNU respectively. Furthermore, gross liver nodules
did not form in any of the Cop rats, whereas all F344 rat livers contained
nodules. Hepatic necrosis caused by DEN during initiation, and CCl4 during
promotion is necessary to stimulate compensatory hepatocyte division. We
demonstrated that these agents do indeed increase serum transaminase levels
and produce histologic evidence of necrosis in Cop rats. In order for liver
foci to grow rapidly in the RH protocol, the surrounding normal hepatocytes
must be mito-inhibited by 2-AAF. We found that the degree of
mito-inhibition of normal hepatocytes by 2-AAF is the same in Cop and F344
rats. These results show that the Cop rat is highly resistant to the
chemical induction of putative preneoplastic liver foci and nodules.
相似文献
59.
60.
PG GIBSON JE STUART J WLODARCZYK LG OLSON MJ HENSLEY 《Journal of paediatrics and child health》1996,32(2):143-147
Objective : Chronic middle ear disease is common in Aboriginal children, and may be linked to nasal inflammation and Eustachian tube dysfunction. The pattern of nasal inflammation is unknown. The study reported here was performed to define the role of allergy and infection in causing nasal inflammation in Aboriginal children with chronic middle ear disease.
Methodology : Thirty-one Aboriginal children aged between 3 and 7 years underwent clinical assessment, audiometry and allergy skin tests. Nasal swabs for bacterial culture and cytology were performed during the winter and again in spring to identify any seasonal variation. A randomized trial of nasal beclomethasone for 8 weeks was conducted in children with abnormal tympanometry to identify the effect of therapy upon nasal cytology.
Results : Twenty-six of the 31 children had abnormal tympanograms. Average hearing levels were reduced in nine children. Pathogenic organisms were isolated from most children: Streptococcus pneumoniae (82%), Haemophilus influenzae (79%), Moraxella catarrhalis (39%) and Staphylococcus aureus (29%). Eight of the 31 children (26%) were atopic. Nasal cytology disclosed a marked neutrophil infiltrate (80% of cells) during the winter, which fell significantly in spring to 52% of cells. Only two subjects had nasal eosinophilia of >10%. There was no effect of beclomethasone on nasal cytology.
Conclusions : Chronic ear disease in Aboriginal children is associated with nasal inflammation, neutrophil infiltration and the presence of bacteria. These features suggest respiratory infection as the main cause of chronic nasal inflammation in Aboriginal children with middle ear disease. There is a seasonal variation in the severity of the nasal infiltrate, consistent with increased infections during winter. Despite a high prevalence of atopy, allergic nasal disease was uncommon. 相似文献
Methodology : Thirty-one Aboriginal children aged between 3 and 7 years underwent clinical assessment, audiometry and allergy skin tests. Nasal swabs for bacterial culture and cytology were performed during the winter and again in spring to identify any seasonal variation. A randomized trial of nasal beclomethasone for 8 weeks was conducted in children with abnormal tympanometry to identify the effect of therapy upon nasal cytology.
Results : Twenty-six of the 31 children had abnormal tympanograms. Average hearing levels were reduced in nine children. Pathogenic organisms were isolated from most children: Streptococcus pneumoniae (82%), Haemophilus influenzae (79%), Moraxella catarrhalis (39%) and Staphylococcus aureus (29%). Eight of the 31 children (26%) were atopic. Nasal cytology disclosed a marked neutrophil infiltrate (80% of cells) during the winter, which fell significantly in spring to 52% of cells. Only two subjects had nasal eosinophilia of >10%. There was no effect of beclomethasone on nasal cytology.
Conclusions : Chronic ear disease in Aboriginal children is associated with nasal inflammation, neutrophil infiltration and the presence of bacteria. These features suggest respiratory infection as the main cause of chronic nasal inflammation in Aboriginal children with middle ear disease. There is a seasonal variation in the severity of the nasal infiltrate, consistent with increased infections during winter. Despite a high prevalence of atopy, allergic nasal disease was uncommon. 相似文献