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81.
Ogata Norihisa; Kouro Taku; Yamada Atsuko; Koike Masamichi; Hanai Nobuo; Ishikawa Takeru; Takatsu Kiyoshi 《Blood》1998,91(7):2264-2271
82.
Impact of reflux esophagitis on the esophageal function before and after laparoscopic fundoplication
Masato Hoshino Nobuo Omura Fumiaki Yano Kazuto Tsuboi Se Ryung Yamamoto Shunsuke Akimoto Takahiro Masuda Hideyuki Kashiwagi Katsuhiko Yanaga 《Esophagus》2018,15(4):224-230
Background
High-resolution manometry (HRM), which is breakthrough testing equipment to evaluate esophageal motor function, was developed in Europe and United State and has garnered attention. Moreover, multichannel intraluminal impedance pH (MII-pH) testing has allowed us to grasp all liquid/gas reflux including not only acid but also non-acid reflux. We examined the impact of the presence of reflux esophagitis (RE) on esophageal motor function before and after laparoscopic fundoplication.Materials and methods
The subjects included 100 patients (male: 63 patients, mean age: 54.1?±?15.8) among 145 patients who underwent laparoscopic fundoplication for GERD associated diseases during a 4-year period from October 2012 to September 2016, excluding 6 patients who underwent further surgery, 32 patients on whom HRM was not performed, 3 patients who had technical errors during testing, and 4 patients for whom the status of RE was unknown. Regarding HRM, Mano Scan from Given Imaging Ltd. was used, and for the analysis, Mano View version 3.0 from the same company was used, after which data was calculated based on the Chicago Classification advocated by Pandolfino et al. Moreover, for the MII-pH testing, Sleuth manufactured by Sandhill Scientific. Inc. was used and automatic analysis was conducted by a computer. Postoperative assessments were conducted 3 months following surgery for all. Data was described in the median value and inter-quartile range, with a statistically significant difference defined as p?<?0.05 by Chi square, Mann–Whitney, and Wilcoxon tests.Results
RE+?group (Los Angeles classification A:B:C:D?=?7:9:16:12 patients) included 44 patients (44%), of older age compared to the RE? group (62 vs. 50 years, p?=?0.012) and a higher Body Mass Index value (24.0 vs. 22.5, p?=?0.045); however, no differences were observed in terms of gender and duration of symptoms. In the preoperative findings on MII-pH, the RE+?group demonstrated significantly longer acid reflux time (4.7 vs. 1.3%, p?=?0.005), while in the HRM findings, the RE? group demonstrated a significantly longer abdominal esophagus (0 vs. 0.4 cm, p?=?0.049) and maintained esophageal body motor function (DCI: 1054 vs. 1407 mmHg s cm, p?=?0.021, Intact peristalsis ratio: 90 vs. 100%, p?=?0.037). As to the comparison of the treatment effect before and after laparoscopic fundoplication (Toupet fundoplication for all), significant improvements were observed in both groups in various parameters regarding reflux including acid reflux time, total number of liquid reflux episodes and total number of reflux episodes. Moreover, for both groups, the total length of the lower esophageal sphincter (LES) (RE+?group: 2.7 vs. 3.2 cm, p?=?0.001, RE? group: 3.0 vs. 3.4 cm, p?=?0.003) and the total length of the abdominal esophagus (RE+?group: 0 vs. 1.6 cm, p?<?0.001, RE? group: 0 vs. 1.8 cm, p?=?0.001) were significantly extended following surgery; however, no change was observed in DCI before and after surgery.Conclusions
Regardless of the presence of RE, cardiac function and LES function were improved following laparoscopic Toupet fundoplication, but no changes were observed in esophageal body motor function.83.
Akiyoshi Kashii Susumu Mitani Y. Kasai M. Mito Takashi Suzuki Toshiya Ito Ryoichi Tsuchiya A. Kaneto K. Tanikawa Masanobu Ishida Tsuyoshi Miura Shiro Hayashi Hiroshi Sano Keisuke Yoshida Hiroshi Ito Nobuo Okazaki Nobu Hattori Ichio Honjo Yasuo Kuroyanagi 《Journal of gastroenterology》1973,8(4):393-401
84.
Takagi S Kinouchi Y Chida M Hiwatashi N Noguchi M Takahashi S Shimosegawa T 《Digestive diseases and sciences》2003,48(10):2091-2094
Telomerase acitivity can be induced in human B lymphocytes by in vitro stimulation of their antigen receptors. To determine whether telomerase activity is induced in vivo, we analyzed telomerase activity in B lymphocytes from the mesenteric lymph nodes of patients with inflammatory bowel disease (IBD), whose lymph nodes are well known to be strongly stimulated, and from those of noninflamed controls. Seven IBD patients and 4 noninflamed controls were enrolled. Telomerase activity was assayed by telomeric repeat amplification protocol with minor modifications. The mesenteric lymph nodes from patients with IBD had stronger telomerase activity than those from controls or peripheral mononuclear cells. Isolation of CD19+ B lymphocytes from these lymph nodes showed that this strong activity resides in this lymphocytes subpopulation. This study provides the evidence that telomerase activity is induced in human B lymphocytes in human inflammatory disease. 相似文献
85.
Fumiaki Yano Nobuo Omura Kazuto Tsuboi Hideyuki Kashiwagi Katsuhiko Yanaga 《Esophagus》2006,3(3):121-124
A 55-year-old man on hemodialysis for diabetic nephropathy suffered from vomiting and hematoemesis. He was diagnosed to have
reflux esophagitis with Los Angeles classification grade D, which was accompanied by a shortened esophagus and stricture.
His reflux esophagitis was proton pump inhibitor resistant. Therefore, we performed laparoscopic Toupet fundoplication. Delayed
esophageal perforation occurred on the ninth day postoperatively, for which he had to be reoperated. To our knowledge, there
is no previous report of delayed esophageal perforation after laparoscopic fundoplication. Esophageal perforation might have
been caused by denudation or attenuation of the abdominal esophagus. 相似文献
86.
Sakabe K Fukuda N Nada T Shinohara H Tamura Y Wakatsuki T Nishikado A Oki T 《Japanese heart journal》2003,44(3):385-393
Although atrial fibrillation is a common arrhythmia, especially in the elderly, little is known about age-related changes in the electrophysiologic properties of the atrium. The aim of this study was to analyze the effect of aging on atrial vulnerability to atrial fibrillation. An electrophysiologic study was performed in 45 patients with no history of atrial fibrillation, Wolff-Parkinson-White syndrome, structural heart disease, or conditions with potential effects on cardiac hemodynamic or electrophysiologic function (15 females; mean age, 52 +/- 18 years; range, 14 to 84 years). The following atrial excitability parameters were assessed: spontaneous or paced (A1) and extrastimulated (A2) atrial electrogram widths, percent maximum atrial fragmentation (A2/A1 x 100), effective refractory period, wavelength index (ERP/A2), and inducibility of atrial fibrillation. Atrial fibrillation was induced in 9 patients. Percent maximum atrial fragmentation was greater (176 +/- 36 vs 137 +/- 26%, P < 0.001) and wavelength index was shorter (2.4 +/- 0.4 vs 3.2 +/- 0.9, P < 0.01) in the patients with than without inducible atrial fibrillation. However, age was similar in patients with and without inducible atrial fibrillation (47 +/- 11 vs 53 +/- 19 years, P = 0.36). Percent maximum atrial fragmentation and effective refractory period directly correlated with age (r = 0.32, P < 0.05 and r = 0.45, P < 0.001, respectively). On the other hand, wavelength index (3.1 +/- 0.9) did not correlate with age (r = -0.05, P = 0.77). This study suggests that the mechanism triggering atrial fibrillation may be very well different between older and younger patients with atrial fibrillation, because younger patients have no marked substrate for atrial fibrillation. 相似文献
87.
88.
Summary Radioactivity in liver nucleic acids increased to a plateau in one week, and decreased slightly after 3 weeks. Radioactivity of nucleic acids in the lungs and kidneys was less than that of liver nucleic acids for the first week, but then gradually increased to the same level as the latter. However, the amount of N7-methylguanine in nucleic acids was 2 to 4 times more in the liver than in the kidneys and lungs after 30 days. The maximal level of N7-methylguanine in DNA was found after 21 days, when approximately 0.1% of guanine residues in liver DNA were methylated. Maximum methylation of RNA was also observed after 21 days. Initially methylation of liver 4S RNA was more than that of ribosomal RNA, but later the extents of methylation to the cytoplasmic RNA species became similar.
The Abbreviations used are DMN dimethylnitrosamine - EDTA ethylenediaminetetraacetic acid disodium - SSC 0.15 M NaCl-0.015 M sodium citrate This work was partly supported by a grant from the Ministry of Education of Japan. 相似文献
Die Bildung von N7-Methylguanin in der Kern-DNS und cytoplasmatischen RNS bei Mäusen während kontinuierlicher oraler Gabe einer Lösung von Dimethyl-nitrosamin-3H
Zusammenfassung Die Aktivität der Nucleinsäuren stieg in der Leber in einer Woche zu einem Plateau an und nahm nach 3 Wochen wieder leicht ab, in Lungen und Nieren war sie in der ersten Woche geringer als in der Leber, stieg aber später zur selben Höhe wie in der Leber. Die Menge von N7-Methylguanin in den Nucleinsäuren war jedoch nach 30 Tagen in der Leber 2-bis 4mal größer als in Nieren und Lunge. Der höchste Spiegel von N7-Methylguanin in DNS fand sich nach 21 Tagen, wenn etwa 0.1% der Guaninreste in der Leber DNS methyliert waren. Maximale Methylierung der RNS wurde ebenfalls nach 21 Tagen beobachtet. Die anfängliche Methylierung von 4S RNA1 war größer als die der ribosomalen RNS; später wurde das Ausmaß der Methylierung ähnlich dem der cytoplasmatischen RNS.
The Abbreviations used are DMN dimethylnitrosamine - EDTA ethylenediaminetetraacetic acid disodium - SSC 0.15 M NaCl-0.015 M sodium citrate This work was partly supported by a grant from the Ministry of Education of Japan. 相似文献
89.
Reactive oxygen species are considered to play a role in the development of diabetes mellitus and its complications. Human MTH1 (mutT homologue 1) has 8-oxo-7,8-dihydrodeoxyguanosine triphosphatase activity, which repairs oxidized forms of dGTP. This enzyme is known to have a thermolabile Met83 variant. We examined whether Val83Met polymorphism of human MTH1 gene is associated with type 1 diabetes mellitus. We recruited 156 type 1 diabetic patients (59 males and 97 females). The polymorphism was analyzed by restriction fragment length polymorphism analysis with Nsi I. The Met/Met genotype at codon 83 was very rare in both control and patient groups. Val/Met genotype tended to be more frequent in the whole type 1 diabetic patients than in controls. When subjects were divided into subgroups according to gender, there were no differences in the genotype and allele frequencies between patients and controls in males. On the other hand, in female type 1 diabetic patients, the Val/Met genotype was more frequent than in female controls (corrected P = 0.102). The Met allele was significantly more frequent in female type 1 diabetic patients than in female controls (corrected P = 0.022). Our results suggested that the Met allele at codon 83 of MTH1 gene might be involved in the development of type 1 diabetes mellitus in the Japanese female population. 相似文献
90.
Oki T Fukuda N Iuchi A Tabata T Manabe K Kageji Y Sasaki M Yamada H Ito S 《Echocardiography (Mount Kisco, N.Y.)》1996,13(6):623-626
A patient of cardiac amyloidosis was found to have mid-to late diastolic retrograde flow from the left atrium (LA) to the pulmonary vein. Congo-red staining was positive for amyloid in the rectal tissue. M-mode and two-dimensional echocardiograms revealed symmetric hypertrophy and typical speckled pattern of the left ventricle (LV). The LV pressure curve showed a dip and plateau configuration during diastole, and end-diastolic pressure was 28 mmHg. In addition, the LV pressure was high at mid-diastole, surpassing the pulmonary capillary wedge pressure from mid-to late diastole. The transmitral flow velocity revealed "restrictive" pattern, and the pulmonary venous flow velocity showed retrograde flow from the LA to the pulmonary vein during mid-diastole and atrial systole. It is suggested that recording of the pulmonary venous flow velocity by transesophageal pulsed Doppler echocardiography is useful for understanding the mechanism of the development of pulmonary congestion or edema. 相似文献