全文获取类型
收费全文 | 4495篇 |
免费 | 168篇 |
国内免费 | 19篇 |
专业分类
耳鼻咽喉 | 49篇 |
儿科学 | 97篇 |
妇产科学 | 73篇 |
基础医学 | 693篇 |
口腔科学 | 80篇 |
临床医学 | 247篇 |
内科学 | 993篇 |
皮肤病学 | 46篇 |
神经病学 | 566篇 |
特种医学 | 155篇 |
外科学 | 674篇 |
综合类 | 25篇 |
预防医学 | 139篇 |
眼科学 | 72篇 |
药学 | 322篇 |
中国医学 | 10篇 |
肿瘤学 | 441篇 |
出版年
2023年 | 18篇 |
2022年 | 51篇 |
2021年 | 66篇 |
2020年 | 39篇 |
2019年 | 55篇 |
2018年 | 67篇 |
2017年 | 67篇 |
2016年 | 61篇 |
2015年 | 62篇 |
2014年 | 63篇 |
2013年 | 121篇 |
2012年 | 209篇 |
2011年 | 225篇 |
2010年 | 147篇 |
2009年 | 140篇 |
2008年 | 235篇 |
2007年 | 263篇 |
2006年 | 275篇 |
2005年 | 281篇 |
2004年 | 324篇 |
2003年 | 331篇 |
2002年 | 291篇 |
2001年 | 63篇 |
2000年 | 67篇 |
1999年 | 57篇 |
1998年 | 95篇 |
1997年 | 81篇 |
1996年 | 56篇 |
1995年 | 54篇 |
1994年 | 59篇 |
1993年 | 71篇 |
1992年 | 61篇 |
1991年 | 60篇 |
1990年 | 57篇 |
1989年 | 51篇 |
1988年 | 33篇 |
1987年 | 34篇 |
1986年 | 36篇 |
1985年 | 41篇 |
1984年 | 37篇 |
1983年 | 33篇 |
1982年 | 17篇 |
1981年 | 23篇 |
1980年 | 27篇 |
1979年 | 27篇 |
1978年 | 19篇 |
1977年 | 26篇 |
1976年 | 17篇 |
1975年 | 13篇 |
1969年 | 12篇 |
排序方式: 共有4682条查询结果,搜索用时 0 毫秒
31.
Evaluation of a Membrane Filter Assay System, Ortho HCV Ab Quik Pack, for Detection of Anti-Hepatitis C Virus Antibody
下载免费PDF全文
![点击此处可从《Journal of clinical microbiology》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Takanari Kodama Satoshi Ichiyama Kumiko Sato Toshi Nada Nobuo Nakashima 《Journal of clinical microbiology》1998,36(5):1439-1440
A simple membrane immunoassay assay system, Quik Pack, for the detection of hepatitis C virus antibody was compared with two enzyme-linked immunosorbent assays (ELISAs) in a study of 600 serum samples. Quik Pack exhibited excellent sensitivity and specificity: 96.0 and 99.7%, respectively, versus the ELISA-2 and 99.7 and 99.4%, respectively, versus the ELISA-3. 相似文献
32.
An invasion-independent pathway of blood-borne metastasis: a new murine mammary tumor model
下载免费PDF全文
![点击此处可从《The American journal of pathology》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Sugino T Kusakabe T Hoshi N Yamaguchi T Kawaguchi T Goodison S Sekimata M Homma Y Suzuki T 《The American journal of pathology》2002,160(6):1973-1980
It is generally believed that active invasion by cancer cells is essential to the metastatic process. In this report, we describe a murine mammary tumor (MCH66) model of metastasis that does not require invasion into the vascular wall of both the primary tumor and the target organ, in this case, the lung. The process involves intravasation of tumor nests surrounded by sinusoidal blood vessels, followed by intravascular tumor growth in the lung, without penetration of the vascular wall during the process. Comparative studies using a nonmetastatic MCH66 clone (MCH66C8) and another highly invasive metastatic cell line (MCH416) suggested that high angiogenic activity and sinusoidal remodeling of tumor blood vessels were prerequisites for MCH66 metastasis. Differential cDNA analysis identified several genes that were overexpressed by MCH66, including genes for the angiogenesis factor pleiotrophin, and extracellular matrix-associated molecules that may modulate the microenvironment toward neovascularization. Our analyses suggest that tumor angiogenesis plays a role in the induction of invasion-independent metastasis. This model should prove useful in screening and development of new therapeutic agents for cancer metastasis. 相似文献
33.
Identification of a novel adhesion molecule involved in the virulence of Legionella pneumophila
下载免费PDF全文
![点击此处可从《Infection and immunity》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Legionella pneumophila is an intracellular bacterium, and its successful parasitism in host cells involves two reciprocal phases: transmission and intracellular replication. In this study, we sought genes that are involved in virulence by screening a genomic DNA library of an L. pneumophila strain, 80-045, with convalescent-phase sera of Legionnaires' disease patients. Three antigens that reacted exclusively with the convalescent-phase sera were isolated. One of them, which shared homology with an integrin analogue of Saccharomyces cerevisiae, was named L. pneumophila adhesion molecule homologous with integrin analogue of S. cerevisiae (LaiA). The laiA gene product was involved in L. pneumophila adhesion to and invasion of the human lung alveolar epithelial cell line A549 during in vitro coculture. However, its presence did not affect multiplication of L. pneumophila within a U937 human macrophage cell line. Furthermore, after intranasal infection of A/J mice, the laiA mutant was eliminated from lungs and caused reduced mortality compared to the wild isolate. Thus, we conclude that the laiA gene encodes a virulence factor that is involved in transmission of L. pneumophila 80-045 and may play a role in Legionnaires' disease in humans. 相似文献
34.
Issei Nishimori Nobuo Moriyama Nobuo Tsuda Akira Shimomura 《Pathology international》1974,24(4):551-567
A 19-year-old female belonging to a family of Alport's syndrome was autopsied and her kidneys were examined in detail light and electron microscopically. The basement membrane was examined chiefly and the laminated thickening and/or splitting, looseness, irregularity and rail-like appearance of lamina densa were found in the glomerular, Bowman's capsular, tubular and interstitial capillary basement membranes. These findings were strongly suggestive of Alport's syndrome, whether or not the particles are seen in the basement membrane. In addition, Japanese reports on Alport's syndrome (total 48 families) were summarized and renal lesions were examined in comparison. It has been said that the prognosis is worse in the male than in the female, but according to our Investigation on case reports in Japan, the prognosis showed no difference between male and female. 相似文献
35.
Kenichiro Karasawa Nobuo Sugiura Yusuke Hori Sakaru Suzuki Junichi Onaya Katsukiyo Sakurai Koji Kimata 《Clinical & experimental metastasis》1997,15(2):83-93
Chondroitin sulfate dipalmitoylphosphatidylethanolamine (CS-PE), when immobilized onto substratum, inhibited the adhesion of B16F10 mouse melanoma cells to fibronectin-coated dishes (anti-adhesion activity). CS-PE showed the most potent anti-adhesion activity for the melanoma cells among various GAG-PEs. CS-PE also inhibited the adhesion of B16F10 cells to Matrigel and the invasion of the cells into Matrigel. In the in vivo system of experimental metastasis, administration of B16F10 cells with CS-PE into C57BL/6 mice significantly inhibited lung metastasis. The inhibition degree of CS or hyaluronic acid-PE was lower than CS-PE. CS-PE administered intravenously into mice before the injection of B16F10 cells also inhibited metastasis. Pretreatment of B16F10 cells with CS-PE caused some but a lower degree of inhibition. When CS-PE was injected intravenously into mice, more binding in the lung was found than when CS was injected. CS-PE but not CS inhibited the retention in the lung of fluorochrome-labeled B16F10 cells when injected intravenously into mice. Since there was no significant effect of CS-PE on the viability and growth of B16F10 cells, the results suggest that CS-PE immobilized onto the subendothelial matrix may prevent melanoma cells from adhering to the subendothelial substrata of lung capillaries and inhibit subsequent invasion processes of metastasis. 相似文献
36.
Nobuo Tsuda Paritosh Roy Chowdhury Tomayoshi Hayashi Masanobu Anami Masachika Iseki Shigehiko Koga Fukuzo Matsuya Hiroshi Kanetake Yutaka Saito Yoshio Horita 《Pathology international》1997,47(11):778-783
Primary renal angiosarcoma is very rare. To our knowledge, only 15 cases have been reported to date. A 77-year-old Japanese man with a unilateral kidney presented with massive hematuria followed by renal failure. A renal tumor was suspected and a left nephrectomy was performed. The histopathological diagnosis was angiosarcoma of the kidney. A hemorrhagic tumor measuring 10 × 5 cm and clotted blood was found in the modularly area. The atypical tumor cells had a sinusoidal and solid appearance, and showed Immunohistochemically positive reactions for some of the endothelial markers. The patient died about 21 months after the nephrectomy and the autopsy revealed massive metastases to the liver and retroperitoneum. One of the differential diagnoses of the case was anglomyolipoma, because the tumor cells were relatively bland in their histological appearance with entrapped fat cells in the pelvic area. Fifteen case reports with titles that included the term 'hemangiosarcoma/anglosarcoma', 'hemangioendothelloma/endothelloma' or 'vascular sarcoma' of the kidney were reviewed and compared to the present case. 相似文献
37.
Koh Nakazawa Nobuo Itoh Hui-Jun Duan Yuichi Komiyama Hidekazu Shigematsu 《Pathology international》1992,42(9):662-666
A 73-year-old Japanese man with a history of partial gastrectomy due to gastric cancer 4 years previously was admitted because of intermittent fever. The patient developed abdominal pain, erythema, and myalgia in addition to the fever during the final clinical course, and died of acute heart failure. Autopsy disclosed atrophy of the left lobe of the liver and acute myocardial infarction. Neither metastasis nor recurrence of the cancer was observed. Small and medium-sized arteries of the visceral organs showed various stages of necrotizing vasculitis with narrowing of the lumina. The vasculitis was most prominent in the left lobe of the liver and in the heart. Narrowing of the portal vein due to portal tract inflammation in addition to vasculitis of the hepatic arteries may have induced ischemia and infarction, which had resulted in atrophy of the left hepatic lobe. Acta Pathol Jpn 42: 662–666, 1992. 相似文献
38.
Fu J Hato M Ohmae H Matsuoka H Kawabata M Tanabe K Miyamoto Y Leafasia JL Chinzei Y Ohta N 《Parasitology research》2000,86(5):345-351
We analyzed the relationships between levels of antibody specific for merozoite surface glycoprotein-1 (MSP1) of Plasmodium falciparum and clinical manifestations in humans. We prepared recombinant MSP1 proteins representing block 3 (M3), block 6 (M6), blocks
1–6 (M1/6), and block 17. When we divided the slide-positive individuals in Guadalcanal into symptomatic and asymptomatic
groups, the former group showed lower IgG levels against M6 and block 17, but not against M3, than did the asymptomatic group
(P < 0.01). The possibility of nonspecific suppression was unlikely, given that the levels of antibody against poliomyelitis
virus observed in the two groups were almost the same. Among the IgG subclasses tested, production of cytophilic IgG3 seemed to be dominant. When we analyzed epitopes recognized by antibodies against block 17, a peptide (SSSNFLGIS) was preferentially
recognized by sera from asymptomatic individuals. These results suggest that clinical symptoms occurring during falciparum
malaria seem to be associated with the development of levels of antibody against particular epitopes on MSP1, which is under
the control of an immunoregulatory mechanism.
Received: 1 October 1999 / Accepted: 21 October 1999 相似文献
39.
Nobukatsu Nemoto Yu Nagase Jiro Abe Hiroshi Matsushima Yasuo Shirai Nobuo Takamiya 《Macromolecular chemistry and physics.》1995,196(7):2237-2249
Side-chain functionalized polysiloxanes were prepared via polymer-analogous esterification of poly[(3-chloroformylpropyl)methylsiloxane] with 4-(4-hydroxyphenylazo)nitrobenzene ( P1 ), 4-[4-(ω-hydroxyalkyloxy)phenylazo]nitrobenzene ( P2 – P4 ), 4-{4-[N-(2-hydroxyethyl)-N-methyl]anilinoazo}nitrobenzene ( P5 ), 4-(4-hydroxypiperidino)nitrobenzene ( P6 ), or 4-[4-(2-hydroxyethyl)piperidino]nitrobenzene ( P7 )., P1 , P3 , P4 and P5 exhibit liquid crystallinity, as deduced from differential scanning calorimetry, polarized microscopic observations and X-ray diffraction measurements. The liquid-crystalline phase of P1 and P5 is a nematic phase, and that of P3 and P4 is a smectic one. The second harmonic generation (SHG) measurement of a spin-coated film of P1 was carried out by the Maker fringe method using a Q-switched Nd:YAG laser (1064 nm). The SHG profile after the heat treatment of a spin-coated film suggests a perpendicular orientation of the mesogenic molecules to the glass substrate. The SH light intensity of a corona-poled film was 20-fold higher than that of a film which was only heated, though no differences were observed in their UV-vis absorption spectra. These findings suggest that the mesogenic-molecular dipole moments are aligned to the same direction in the crystalline or liquid-crystalline phase by a poling treatment. 相似文献
40.
Statistical detection of HLA and disease association 总被引:1,自引:0,他引:1
Katsuhirci Fukuda Kazuaki Sugawa Akemi Wakisaka Junko Mokiuchi Nobuo Matsuura Yoshiharu Sato 《Tissue antigens》1985,26(2):81-86