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991.
我国散发性戊型肝炎病毒细胞培养分离株的鉴定 总被引:3,自引:0,他引:3
我国散发性戊型肝炎病毒细胞培养分离株的鉴定100850北京军事医学科学院微生物流行病研究所苑锡同,李晓萸,黄如统,李德荣关键词肝炎病毒,戊型;病毒细胞培养;病毒鉴定;聚合酶链反应;核酸杂交中国图书资料分类号Q939.4我国戊型肝炎存在着流行和散发两种... 相似文献
992.
X. Wang W. Sun X. Zhu X. Wu L. Li S. Zhu T. Du Y. Liu N. Niu Y. Wang Y. Liu 《European journal of neurology》2008,15(8):827-830
Background and purpose: Prion protein (PrP) predominantly localized at synapses can modulate neuronal excitability. The prion protein gene ( PRNP ) has been considered one of the candidate genes that play a role in seizure susceptibility. A recent study demonstrated that the 129V allele in the PRNP gene was associated with susceptibility to temporal lobe epilepsy (TLE) in female patients in an Italian population. We screened variations in the open-reading frame (ORF) of the PRNP gene and also replicated the association of the M129V polymorphism with TLE in a Han Chinese population.
Methods: The M129V polymorphism was genotyped in 320 MTLE patients and 558 non-epilepsy controls. All subjects were Han Chinese.
Results: No novel polymorphism in the ORF of the PRNP gene was detected. Differences in the genotype distributions and allele frequencies of this polymorphism between cases and controls were insignificant ( P = 0.24). Further analysis with stratification of the results by gender or age and analysis of clinical features in relation to M129V genotypes also yielded negative findings.
Conclusions: The present study provides evidence that the M129V polymorphism in the PRNP gene is not associated with MTLE in a Han Chinese population. 相似文献
Methods: The M129V polymorphism was genotyped in 320 MTLE patients and 558 non-epilepsy controls. All subjects were Han Chinese.
Results: No novel polymorphism in the ORF of the PRNP gene was detected. Differences in the genotype distributions and allele frequencies of this polymorphism between cases and controls were insignificant ( P = 0.24). Further analysis with stratification of the results by gender or age and analysis of clinical features in relation to M129V genotypes also yielded negative findings.
Conclusions: The present study provides evidence that the M129V polymorphism in the PRNP gene is not associated with MTLE in a Han Chinese population. 相似文献
993.
目的比较痔上黏膜环切钉合术(PPH)与Milligan—Morgan术(MMH)治疗Ⅲ、Ⅳ度内痔的远期疗效。方法按照完全随机分组原则将100例Ⅲ、Ⅳ度内痔患者分别采用PPH(PPH组,42例)和MMH(MMH组,58例)手术,观察并比较两组患者术后的远期疗效差异。结果PPH组与MMH组术后2年比较,在出现肛门溢液(2.38%比20.69%,P=0.007)、皮赘(9.52%比25.86%,P=0.040)和细便(2.38%比18.97%,P=0.027)方面PPH组术后少见,总的并发症发生率(9.52%比25.86%,P=0.040)和总排便功能异常(9.52%比29.31%,P=0.017)方面PPH组术后也少见,两组差异有统计学意义。在痔脱垂复发(14.29%比10.34%,P=0.549)、症状改善满意度(92.86%比87.93%,P=0.636)和总的症状复发(19.05%比25.86%,P=0.424)方面两组差异无统计学意义。结论PPH与Milligan-Morgan术治疗Ⅲ、Ⅳ度内痔的远期疗效无显著差异,但PPH安全、并发症少、对排便功能影响小。 相似文献
994.
995.
A gas-solid chromatographic system using tandem silica gel and molecular sieve columns is described for the measurement of hydrogen, carbon dioxide, oxygen, and nitrogen in samples of respiratory gases. This system has a detection limit of 2 ppm of hydrogen in a 1 ml sample and can measure 120 ppm H2 and 5% CO2 with relative standard deviations of 1.3% and 1.7%, respectively. Improved sample storage and withdrawal techniques are described that give reproducible values for up to 6 weeks after collection. Finally we show that normalization of breath hydrogen values to an alveolar concentration, using the observed carbon dioxide concentrations, substantially reduces the range and variance of apparent H2 concentrations in human subjects. Normalization eliminates the need for rebreathing or end-expiratory collection techniques and substantially increases the reliability and clinical utility of hydrogen breath measurements in noninvasive tests of carbohydrate malabsorption. 相似文献
996.
31 cases of atherosclerosis (AS) were treated with Jiang Zhi Tong Mai Fang ([symbol: see text], formula of JZTMF), and its effect was compared with 30 cases treated with lovastatin in the control group. Clinically, the JZTMF formula showed an effect of regulating blood lipids, and therefore it was antiatherosclerotic. The mechanism is, probably, restoration of the function of endothelial cells (EC) by increasing the synthesis of 6-keto-PGF1 alpha and decreasing the release of endothelin (ET) as evidenced in the experimental study. 相似文献
997.
Li Shao-qiu 李绍裘 I-Iua Ming花明 Cao Kai-heng曹开恒and Niu Yi-min牛义民Department of Surgery Zunyi Medical. CoZlege Zunyi 《中华医学杂志(英文版)》1985,98(12):887-888
Valve failure is rate after using glutaraldehyde
stabilized bovine pericardial bioprosthesis in the
mitral position.i This article reports a case of spon-
taneous disruption of bovine pericardial xenograft
after mitral valve replacement five years after opera-
tion. 相似文献
998.
Liu Yan-fang刘彦仿 Hu Ming-qin胡明钦 Departme''nt of Patholog''y th Militar''g Medical College Niu Shu-miao牛恕淼 Department of Pathology Xian Medical Col.lege Xian Wu Yan-ling吴延玲Department of Patlzology Shaanxi Medical Coll ege for Advanced Stud''y Xia}z 《中华医学杂志(英文版)》1984,97(6):429-432
ChroLnic lymphocytic thyroiditis and lympho-
cyte infiltration were studied in 2,053 cases of
endemic goiter. Only 7 cases of chronic lympho-
cytic thyroiditis and 3 0f focal thyroiditis
showed diffused enlargement of thyroid. Among
2,053 cases, 150 had thyroiditis (7.3%), of whom
137 (7.1%) were accompanied by nodular goiters.
If focal thyroiditis was excludetl, the incidence
rate of chronic lymphocytic thyroiditis would be
2.39T%
The increased incidence of Hashimoto's
disease was considered to he due to the decreased
incidence of endemic goiter and the neglect of
chronic lymphocytic thyroiditis accompanied by
nodular goiters. Thyroiditis occurs in the sur-
rounding tissues of the nodule, and it is why
the changes in thyroiditis were overlooked iii
excised nodules. 相似文献
999.
Yao Chong-zheng么崇正 Zeng Xian-jiu曾宪九and Niu Zhan-po牛占坡CapitaZ Hospital Chinese Academ''yo Medical Sciences Beijing 《中华医学杂志(英文版)》1984,97(1):57-60
Intraoperative rapid insulin assay has been
successfully used for a year in our hospital for
monitoring completeness of insulinoma excision
during operations on 5 patients* According to
our experience, portal blood insulin levels fell
sharply shortly after removal of insulinoma.
Wicth rapid immunoreactive insulin assay9 the
time between sample taking and final reading
was about 45 minutes. As blood sugar monitoring
is influenced by many factors and correct result
can only be obtained in an average of 70 minutes
or even longer after complete remoival of tumor,
rapid immunoreactive insulin assay has evident
advantages over blood sugar monitoring. 相似文献
1000.
氧化型低密度脂蛋白诱导主动脉和心内膜内皮细胞凋亡 总被引:1,自引:1,他引:0
目的:研究氧化型低密度脂蛋白(ox-LDL)诱导血管和心内膜内皮细胞凋亡。方法:用超速离心法分离健康人血浆低密度脂蛋白(LDL),以CuSO410μmol.L^-1氧化,观察ox-LDL对培养新生小牛主动脉内皮细胞及心内膜细胞的损伤作用,琼脂糖凝胶电泳和Hoechst33258荧光密度法定性与定量分析DNA降解,结果:ox-LDL诱导血管内皮细胞及以内膜细胞典型凋亡形态学改变,DNA降解呈时间和剂 相似文献