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61.
62.
Zainab Mohammedi Golwala Hardik Shah Neeraj Gupta V Sreenivas Jacob M Puliyel 《African health sciences》2016,16(2):356-362
BackgroundThrombocytopenia has been shown to predict mortality. We hypothesize that platelet indices may be more useful prognostic indicators. Our study subjects were children one month to 14 years old admitted to our hospital.AimTo determine whether platelet count, plateletcrit (PCT), mean platelet volume (MPV) and platelet distribution width (PDW) and their ratios can predict mortality in hospitalised children.MethodsChildren who died during hospital stay were the cases. Controls were age matched children admitted contemporaneously. The first blood sample after admission was used for analysis. Receiver operating characteristic (ROC) curve was used to identify the best threshold for measured variables and the ratios studied. Multiple regression analysis was done to identify independent predictors of mortality.ResultsForty cases and forty controls were studied. Platelet count, PCT and the ratios of MPV/Platelet count, MPV/PCT, PDW/Platelet count, PDW/PCT and MPV × PDW/Platelet count × PCT were significantly different among children who survived compared to those who died. On multiple regression analysis the ratio of MPV/PCT, PDW/Platelet count and MPV/Platelet count were risk factors for mortality with an odds ratio of 4.31(95% CI, 1.69–10.99), 3.86 (95% CI, 1.53–9.75), 3.45 (95% CI, 1.38–8.64) respectively. In 67% of the patients who died MPV/PCT ratio was above 41.8 and PDW/Platelet count was above 3.86. In 65% of patients who died MPV/Platelet count was above 3.45.ConclusionThe MPV/PCT, PDW/Platelet count and MPV/Platelet count, in the first sample after admission in this case control study were predictors of mortality and could predict 65% to 67% of deaths accurately. 相似文献
63.
Isolation of a lytic bacteriophage against virulent Aeromonas hydrophila from an organized equine farm
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64.
Pelvic fractures carry a considerable risk for morbidity and mortality. Half or more of the early deaths in these patients have been attributed directly to haemorrhage. The transfusional requirements are four times higher for open pelvic fractures compared with a similar group of closed pelvic fractures. The loss of the tamponade effect by disruption of the pelvic soft tissues and the energy imparted play a central role in this potentially life threatening situation. We reported a case of open pelvic fracture in which persistent haemorrhage was stopped by giving recombinant activated coagulation factor VIIa as our last resort. 相似文献
65.
66.
Habituation is a nonassociative learning mechanism, in which an initial response toward repeated stimuli gradually wanes. This is amongst the simplest and most widespread forms of behavioral plasticity. So far, neither the underlying molecular mechanisms nor the precise neural networks of habituation are well understood. We have developed a novel paradigm to quantify habituation of the olfactory jump reflex in Drosophila. We present data demonstrating several behavioral properties of this phenomenon, generally observed in other species. We also show that the dunce and rutabaga memory mutants behave abnormally in this assay, suggesting that this assay might be used in behavioral screens for new mutants with defects in this simpler form of behavioral plasticity. 相似文献
67.
Liu Y Purvis J Shih A Weinstein J Agrawal N Radhakrishnan R 《Annals of biomedical engineering》2007,35(6):1012-1025
We describe a hierarchical multiscale computational approach based on molecular dynamics simulations, free energy-based molecular
docking simulations, deterministic network-based kinetic modeling, and hybrid discrete/continuum stochastic dynamics protocols
to study the dimer-mediated receptor activation characteristics of the Erb family receptors, specifically the epidermal growth
factor receptor (EGFR). Through these modeling approaches, we are able to extend the prior modeling of EGF-mediated signal
transduction by considering specific EGFR tyrosine kinase (EGFRTK) docking interactions mediated by differential binding and
phosphorylation of different C-terminal peptide tyrosines on the RTK tail. By modeling signal flows through branching pathways
of the EGFRTK resolved on a molecular basis, we are able to transcribe the effects of molecular alterations in the receptor
(e.g., mutant forms of the receptor) to differing kinetic behavior and downstream signaling response. Our molecular dynamics
simulations show that the drug sensitizing mutation (L834R) of EGFR stabilizes the active conformation to make the system
constitutively active. Docking simulations show preferential characteristics (for wildtype vs. mutant receptors) in inhibitor
binding as well as preferential enhancement of phosphorylation of particular substrate tyrosines over others. We find that
in comparison to the wildtype system, the L834R mutant RTK preferentially binds the inhibitor erlotinib, as well as preferentially
phosphorylates the substrate tyrosine Y1068 but not Y1173. We predict that these molecular level changes result in preferential
activation of the Akt signaling pathway in comparison to the Erk signaling pathway for cells with normal EGFR expression.
For cells with EGFR over expression, the mutant over activates both Erk and Akt pathways, in comparison to wildtype. These
results are consistent with qualitative experimental measurements reported in the literature. We discuss these consequences
in light of how the network topology and signaling characteristics of altered (mutant) cell lines are shaped differently in
relationship to native cell lines.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users.
These authors contributed equally 相似文献
68.
Gurpreet Kaur Varsha Dogra Rajeev Kumar Sandeep Kumar Gaurav Bhanjana Neeraj Dilbaghi Nitin Kumar Singhal 《International journal of pharmaceutics》2018,535(1-2):95-105
In the present study, we have synthesized mixed cuprous/copper oxide nanosuspensions by metallosurfactant based microemulsion technique. Three metallosurfactants were synthesized which includes two non-ionic double chained metallosurfactants with C12, C16 chains with coordinated copper i.e. Cudda and Cuhexa, respectively. Another cationic double chained metallosurfactant with loosely bound metal (Cuctac) was also prepared. The prepared metallocomplexes were characterized using FTIR, elemental analysis, and NMR. The effect of the position of metallosurfactant in microemulsion on the fabrication and properties of nanosuspensions was elucidated. In this method, no external reducing agent and capping agent were added and tween 80 acted both as reducing and stabilizing agent for the nanoparticles. The synthesized nanoparticles were characterized and it was observed that mixed copper and cuprous oxide particles are present in colloidal suspension for all the three studied metallosurfactants. The kinetics of formation of mixed copper/cuprous oxide nanosuspensions (Ns) and their stability was estimated using Uv-visible spectroscopy. Further, the binding and interactions of copper nanosuspensions with calf Thymus DNA (CT-DNA) were assessed using Uv–vis spectroscopy, circular dichroism and gel electrophoresis. Additionally, the antioxidant activity of the Cu Ns was checked using DPPH assay. The role of positive charge on nanoparticles as evaluated from Zeta potential was responsible for DNA affinity. The DNA conformational changes in the presence of nanosuspensions and relevant scavengers were investigated. Further, the anti-proliferative activity of copper Ns was assessed using HeLa cells and Cuhexa derived Ns were proved to be active with highest activity at a low concentration and were nontoxic towards normal cell lines. In summary, this work demonstrates a softer approach for the synthesis of copper nanosuspensions with a size range of 2–5?nm and evaluated the role of type and structure of metallosurfactant on size, stability of particles and anti-proliferative activity. 相似文献
69.
Galhotra V Pandit IK Srivastava N Gugnani N 《Journal of dentistry for children (Chicago, Ill.)》2007,74(1):73-75
The purpose of this paper was to report an unusual case of multirooted permanent maxillary canine. A 16-year-old female patient presented with pain and swelling related to the upper right permanent canine. Radiographic examination revealed a multirooted permanent maxillary canine--an unusual finding. Endodontic treatment was performed after amputation of 2 extra roots, and then the tooth was intentionally reimplanted. The prevalence of birooted permanent mandibular canines in the Japanese population has been reported, but the prevalence of this 3-rooted maxillary canine is still unknown. This report also states the potential etiological factors, effects on the developing dentition, and various treatment options for the multirooted maxillary permanent canine. 相似文献
70.
Amarjeet Bisla Rupali Rautela Vinay Yadav Praveen Singh Abhishek Kumar Subrata Ghosh Ajay Kumar Sadhan Bag Brijesh Kumar Neeraj Srivastava 《Andrologia》2020,52(9):e13709
The study consisted of application of anti-ubiquitin antibodies (Abs)-coated iron oxide-nanoparticles (IONPs) for minimisation of oxidative stress to contemporary live spermatozoa from the raw semen. Round-shaped IONPs (12.09 ± 0.91 nm) after two-stage functionalisation (silanisation and pegylation) were conjugated with Abs. Four aliquots from each of the 24 ejaculates (4 buffalo bulls) formed Control (Group I) and treatment (II, III and IV) groups; each containing 150 ± 25 million dead/damaged spermatozoa. IONPs-Abs complex were added at ratio of 1:1 (0.5 µg/ml), 1:2 (1.0 µg/ml) and 1:4 (2.0 µg/ml), respectively, in Groups II, III and IV. The semen quality parameters showed improvement at lag-stage (post-nano-purification before processing for cryopreservation). The mean post-thaw motility (%) in Group IV was found to be greater (p < .05) than Group I. Moreover, the overall DNA integrity (%) at post-thaw stage was improved in the nano-purified semen samples. The value of malondialdehyde was greater (p < .001) in Group I than Groups II, III and IV. The mean total antioxidant capacity and superoxide dismutase (U/mg protein) activity values in Group IV was greater (p < .05) than Group I. The study results show that IONPs conjugated with anti-ubiquitin Abs at 2.0 µg/ml can be an effective dose for depletion of dead/damaged spermatozoa from buffalo ejaculates to minimise oxidative stress. 相似文献