全文获取类型
收费全文 | 2578篇 |
免费 | 463篇 |
国内免费 | 17篇 |
专业分类
耳鼻咽喉 | 43篇 |
儿科学 | 84篇 |
妇产科学 | 122篇 |
基础医学 | 284篇 |
口腔科学 | 133篇 |
临床医学 | 781篇 |
内科学 | 487篇 |
皮肤病学 | 78篇 |
神经病学 | 137篇 |
特种医学 | 110篇 |
外科学 | 269篇 |
综合类 | 31篇 |
一般理论 | 1篇 |
预防医学 | 240篇 |
眼科学 | 37篇 |
药学 | 119篇 |
中国医学 | 16篇 |
肿瘤学 | 86篇 |
出版年
2023年 | 5篇 |
2022年 | 47篇 |
2021年 | 79篇 |
2020年 | 40篇 |
2019年 | 44篇 |
2018年 | 128篇 |
2017年 | 148篇 |
2016年 | 164篇 |
2015年 | 175篇 |
2014年 | 148篇 |
2013年 | 198篇 |
2012年 | 174篇 |
2011年 | 208篇 |
2010年 | 138篇 |
2009年 | 161篇 |
2008年 | 161篇 |
2007年 | 175篇 |
2006年 | 102篇 |
2005年 | 105篇 |
2004年 | 90篇 |
2003年 | 73篇 |
2002年 | 53篇 |
2001年 | 36篇 |
2000年 | 40篇 |
1999年 | 33篇 |
1998年 | 32篇 |
1997年 | 27篇 |
1996年 | 22篇 |
1995年 | 13篇 |
1994年 | 10篇 |
1993年 | 11篇 |
1992年 | 21篇 |
1991年 | 14篇 |
1990年 | 25篇 |
1989年 | 18篇 |
1988年 | 18篇 |
1987年 | 16篇 |
1986年 | 7篇 |
1985年 | 19篇 |
1984年 | 12篇 |
1982年 | 7篇 |
1981年 | 3篇 |
1980年 | 5篇 |
1979年 | 3篇 |
1978年 | 4篇 |
1974年 | 5篇 |
1973年 | 5篇 |
1971年 | 4篇 |
1969年 | 6篇 |
1960年 | 3篇 |
排序方式: 共有3058条查询结果,搜索用时 0 毫秒
11.
Seventy five patients with acute and chronic active hepatitis (CAH) were studied by indirect immunofluorescence with monoclonal antibodies for the presence of hepatitis B surface antigen (HBsAg) on peripheral blood mononuclear cells (PBMC). The viral surface antigen was detected in the PBMC of all the patients with hepatitis B virus (HBV)-induced CAH and in acute patients with more than 2 months of evolution. No HBsAg was detected in the samples obtained from 12 normal controls or from 14 non-A, non-B CAH patients. Analysis of PBMC subsets revealed that HBsAg was present in non-T cells; dual fluorescence studies showed HBsAg on surface Ig-positive lymphocytes. The binding of anti-HBs monoclonal antibodies was higher than that of a goat anti-HBs serum, and the highest reactivity was observed with an antibody against the pre-S(2)-region sequence. Both HBsAg and hepatitis B core antigen (HBcAg) were also detected in lysates of PBMC by dot blot analysis. 相似文献
12.
Ernesto Mendes Açucena Raphael Norma G.S. Mota Nelson F. Mendes 《The Journal of allergy and clinical immunology》1974,53(4):223-229
Eighty-nine patients with leprosy, 65 classified as lepromatous and 24 as tuberculoid, were examined in this study. Skin test responses to protein antigens and dinitrochlorobenzene (DNCB) were depressed in lepromatous patients compared to controls. Tuberculoid patients did not exhibit a significant depression to microbial antigens, but they showed a definite depression in the ability to be sensitized with DNCB. The transfer of delayed hypersensitivity reactions to tuberculin, trichophytin, and lepromin (Fernandez and Mitsuda reactions) was accomplished in lepromatous and indeterminate leprosy patients using viable lymphocytes from donors presenting positive reactions to these antigens. The lepromin reaction was also transferred to patients with South American blastomycosis and cutaneous leishmaniasis. The positive reactions of adoptive immunity were confirmed by histologic examination of skin biopsies. 相似文献
13.
Sousa MM Ferrão J Fernandes R Guimarães A Geraldes JB Perdigoto R Tomé L Mota O Negrão L Furtado AL Saraiva MJ 《Laboratory investigation; a journal of technical methods and pathology》2004,84(7):865-873
Familial amyloid polyneuropathy (FAP) is characterized by deposition of mutated transthyretin (TTR) in the peripheral nervous system. Prior to amyloid fibrils, nonfibrillar TTR aggregates are deposited inducing oxidative stress with increased nitration (3-NT). As the major source of TTR is the liver, liver transplantation (LT) is used to halt FAP. Given the shortage of liver donors, domino LT (DLT) using FAP livers is performed. The correlation between TTR deposition in the skin and nerve was tested in biopsies from normal individuals, asymptomatic carriers (FAP 0) and FAP patients; in FAP 0, nonfibrillar TTR was observed both in the skin and nerve in the same individuals; in patients, amyloid was detected in both tissues. The occurrence of amyloidosis in recipients of FAP livers was evaluated 1-7 years after DLT: TTR deposition occurred in the skin 3 years after transplantation either as amyloid or aggregates; in one of the recipients, fibrillar TTR was present in the epineurium 6 years after DLT. Deposits were scarce and 3-NT immunostaining was irrelevant. Nerve biopsies from DLT recipients had no FAP-related neuropathy. Our findings suggest that TTR amyloid formation occurs faster than predicted and that TTR of liver origin can cross the blood-nerve barrier. Recipients of FAP livers should be under surveillance for TTR deposition and tissue damage. 相似文献
14.
Preferential expansion of Ly-1 B and CD4 CD8 T cells in the polyclonal lymphocyte responses to murine T.cruzi infection 总被引:4,自引:0,他引:4
Minoprio P.; Bandeira A.; Pereira P.; Santos T. Mota; Coutinho A. 《International immunology》1989,1(2):176-184
Acute murine infection with T.cruzi results in polyclonal lymphocyteresponses manifested by blast transformation of a large fractionof B, CD4+, and CD8+ cells. We describe here the finding ofsignificant increases in the splenic representation of minorpopulations, Ly-1+ B cells and CD4-CD8- T cells. These lymphocytepopulations might play an important role in the host response,as shown by T.cruzi infection of hosts that had been lethallyirradiated and reconstituted with autologous bone marrow. Underthese conditions, the splenic polyclonal PFC responses are nearlyabrogated, and not restored by the transfer of syngeneic peritonealcells which, however, reconstitute T15 idiotype production inthe same hosts. Control levels of PFC responses, however, arereconstituted by transfer of syngeneic splenic T cells. Sincebone marrow-reconstituted animals contain normal numbers ofCD4+ and CD8+ T cells which are actually activated by infection,these results suggest the participation of other T cell populationsin the host response to infection, as also suggested by themarked increases in T cell receptor and messages detectedin the spleen of infected animals. The implications of thesefindings in immunopathology of Chagas' disease are discussed. 相似文献
15.
A cell separation technique was designed which was based on the reaction of protein A of Staphylococcus aureus (SpA) with the Fc regions of IgG antibodies. SpA covalently linked to Sepharose 6MB is able to fix antibody-bearing cells, while non-fixed cells are removed by washing. The fixed cells can be released by dog IgG or by mechanical treatment. As an example of the use of the technique, Ig-bearing cells were isolated from mouse spleen cells treated with rabbit IgG anti-mouse Ig antibody; the purity of the Ig-bearing lymphocytes so isolated was better than 90%. The viability and the ability of the cells to shed the antibodies from their surface were not significantly impaired by the fractionation method. The technique can be generally applied to cell separation providing IgG antibodies against specific surface markers are available. 相似文献
16.
17.
Heterogeneity of guinea-pig homocytotropic antibodies 总被引:5,自引:0,他引:5
It is suggested that guinea-pigs produce three different homocytotropic antibodies that can be distinguished by their physicochemical and biological properties. One has the properties of an IgG antibody, is heat and mercaptoethanol resistant and persists in the passively sensitized skin for not more than 72 hours. The other has the properties of an IgE antibody, is heat and mercaptoethanol labile and persists in the passively sensitized skin for at least 20 days. The third antibody is heat stable and mercaptoethanol labile and persists in the passively sensitized skin for more than 72 hours but less than 1 week. This antibody was partially separated from γ1 antibody by chromatography on DEAE cellulose and may represent a distinct segment of the γ1 antibody population. All three antibodies were able to sensitize guinea-pig mast cells and cause degranulation of these cells on reaction with antigen. Antihistamines produced complete inhibition of PCA reactions induced with all three types of antibodies but were much less efficient in inhibition PCA reactions induced with γ1 antibody obtained from hyperimmune serum. 相似文献
18.
19.
20.
Inhibition of poly(ADP-ribose) polymerase attenuates the severity of acute pancreatitis and associated lung injury 总被引:4,自引:0,他引:4
Mota RA Sánchez-Bueno F Saenz L Hernández-Espinosa D Jimeno J Tornel PL Martínez-Torrano A Ramírez P Parrilla P Yélamos J 《Laboratory investigation; a journal of technical methods and pathology》2005,85(10):1250-1262
The severity of acute pancreatitis results from the transmigration and activation of leukocytes within the pancreas and the local synthesis and release of proinflammatory-soluble mediators that transform a local injury into a systemic inflammatory response. Poly(ADP-ribose)polymerase-1 (PARP-1) is a nuclear DNA-binding protein that has been shown to play a relevant role in cell necrosis and organ failure in various diseases associated with inflammation. Therefore, we set out to investigate whether the genetic deletion of PARP-1 or PARP-2 (a new member of the PARP family) genes, or pharmacological inhibition of PARP activity might affect the development and severity of acute pancreatitis and pancreatitis-associated lung injury. Secretagogue-induced acute pancreatitis was achieved by 12 hourly intraperitoneal injections of cerulein in mice deficient in PARP-1 or PARP-2 genes, and wild-type (WT) littermate mice untreated or treated with PARP activity inhibitors. The severity of pancreatitis was assessed by measurements of serum amylase, lipase, interleukin-1beta and IL-6, pancreatic water content, histologic grading and pancreas myeloperoxidase (MPO) activity. Lung injury was evaluated by quantifying MPO activity and morphological changes. We found that the severity of acute pancreatitis and pancreatitis-associated lung injury was significantly attenuated in mice lacking PARP-1, but not PARP-2, compared with WT mice. Interestingly, administration of PARP inhibitors, 3-aminobenzamide or PJ34 (N-(6-oxo-5,6-dihydro-phenanthridin-2-yl)-N,N-dimethyacetamide HCl), in WT mice markedly decreased acute pancreatitis severity and pulmonary-associated injury in a larger extension than genetic deletion of PARP-1. Our results support the potential therapeutic application of PARP inhibitors in the development and severity of acute pancreatitis and associated lung injury. 相似文献