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991.
急性非淋巴细胞白血病中MGMT与p53基因突变的研究 总被引:1,自引:0,他引:1
目的 探讨肿瘤抑制基因p53、MGMT在急性非淋巴细胞白血病(ANLL)中的改变。方法 用聚合酶链反应-单链构象多态性(PCR-SSCP)技术对31例ANLL患者、22例正常人p53基因第5、6、7、8外显子和MGMT基因第1、4外显子进行突变分析。结果 31例ANLL中5例存在p53基因突变、4例存在MGMT基因突变,其中2例p53与MGMT同时发生突变。结论 p53基因突变在白血病发生中起一定 相似文献
992.
中俄,中哈边境地区莱姆病自然疫源地调查研究 总被引:7,自引:0,他引:7
目的:调查边境地区莱杂姆病和自然疫源地的状况。方法;对血清学、病原学、宿主、媒介,病例和地理景观方面进行调查。结果:用IFA和ELISA法共检测人畜血清1501份,从199份人血清中检出抗体阳性18份,阳性率为9.05%,并发现莱姆病典型患者15例;检查马血清20份,阳性5份,阳性率为25.00%;检查牛血清399份,阳性69份,阳性率17.29%;检查羊血清883份,阳性199份,阳性率22.5 相似文献
993.
准分子激光原位角膜磨镶术后屈光回退与术中角膜床厚度的关系 总被引:6,自引:2,他引:4
目的 探讨准分子激光原位角膜磨镶术(excimer laser insitu keratomileusis LASIK) 矫治高度近视后屈光回退与术中角膜床厚度的关系。方法 对476 例938 只眼在我院接受Lasik 治疗的患者中,发生屈光回退的病例96 例,189 只眼与未发生屈光回退的病例380 例749 只眼术中角膜床厚度及术前、术后1 w k 、1 、3 、6 m o 屈光状态的资料进行对比分析。结果 有屈光回退眼组比无屈光回退眼组平均角膜床厚度小;屈光回退眼组屈光回退程度( 术后6 mo 时) 与角膜床厚度呈负相关系,上述结果经统计学检验均有显著性差异( P < 001) ;有屈光回退眼组术后早期(1 wk) 屈光状态略成过矫,但其后屈光回退程度在0 .5a 内呈加重趋势。结论 高度近视准分子激光原位角膜磨镶术中的角膜床厚度是影响术后屈光稳定性的重要因素 相似文献
994.
复发性单纯疱疹性角膜炎患者血清IL-2及SIL-2R水平的研究 总被引:12,自引:1,他引:11
目的研究复发性单纯疱疹性角膜炎患者血清白细胞介素-2(IL-2)及其可溶性白细胞介素-2受体(SIL-2R)水平。方法本文测定了30例复发性单纯疱疹性角膜炎患者(实验组)和25例健康人(对照组)血清白细胞介素-2(IL-2)及其可溶性白细胞介素-2受体(SIL-2R)的水平。结果对照组IL-2的水平为5.41±1.45mg·L-1,实验组IL-2R水平为2.80±1.47mg·L-1,2组比较t=2.90,P<0.01,说明复发性单纯疱疹性角膜炎患者血清IL-2水平较对照组明显降低。对照组SIL-2R水平为90.86±17.90mg·L-1,实验组SIL-2R水平为171·46±19.30mg·L-1,2组比较t=5.17,P<0.01。复发性单纯疱疹性角膜炎患者血清SlL-2R水平较对照组显著升高。结论复发性单纯疱疹性角膜炎患者体内免疫功能紊乱,血清IL-2活性降低、SlL-2R水平升高可能是其原因之一,这也为免疫治疗提供了一定依据。 相似文献
995.
目的实验性自身免疫性前葡萄膜炎(EAAU)是研究人前葡萄膜炎的有用模型。本研究旨在观察前房相关免疫偏离(ACAID)能否阻止EAAU发生。方法将溶于磷酸盐缓冲液(PBS)的牛黑色素蛋白(BMP)注射于大鼠右眼前房;对照组动物仅注射PBS。7d后,使用添加完全福氏佐剂(CFA)的BMP和百日咳毒素免疫所有动物。通过临床观察和组织病理学检测葡萄膜炎的严重程度和发病率。通过由注射BMP刺激的足垫水肿反应评估迟发型超敏反应(DTH)。结果与对照组相比,预先眼内注射BMP的大鼠,其EAAU的严重程度和发病率减低,且DTH反应受到显著抑制。结论这些资料提示ACAID可以用来阻止EAAU的发生。 相似文献
996.
增生性玻璃体视网膜病变玻璃体切割物的细胞凋亡观察 总被引:1,自引:0,他引:1
目的观察增生性玻璃体视网膜病变玻璃体切割物的细胞凋亡情况 。方法收集60例不同分级的增生性玻璃体视网膜病变患者的玻璃体切割物,核苷酸末端转移酶介导dUTP缺口翻译法(TdT-mediated dUTP nick end label ling method,TUNEL法)标记凋亡细胞,光镜观察。结果60例患者的玻璃体切割物均有细胞凋亡的特征性改变,随着病变程度的加重,非色素细胞凋亡总数逐渐减少,并出现色素细胞凋亡。结论增生性玻璃体视网膜病变存在不同类型细胞的凋亡,细胞凋亡是调控其病变程度的重要机制之一。(中华眼底病杂志,1999,15:81-83) 相似文献
997.
目的 :探讨B超在近视眼巩膜后葡萄肿中的应用价值。方法 :用B型超声仪检查 >3.0 0D的 38例76眼进展性近视眼的巩膜后葡萄肿的发生情况。结果 :76眼中 5 8眼发生后葡萄肿。其中各级后葡萄肿的平均屈光度及眼轴差异均有非常显著性意义 (P <0 .0 1和P <0 .0 0 1)。 1~ 4级后葡萄肿与 0级葡萄肿的弧形斑发生率比较差异有非常显著性意义 (P <0 .0 1)。结论 :B超是诊断巩膜后葡萄肿最安全、快速、可靠的方法 ,对近视眼的预后判断有着重要作用。近视眼的屈光度和眼轴随巩膜后葡萄肿的加重而增加 ,眼底弧形斑的形成与后葡萄肿的形成有密切关系 相似文献
998.
Summary A reliable method for obtaining high-resolution R-banded chromosomes from lymphoblastoid cell lines is described. The cell cultures are subjected to S-phase synchronization in the presence of excess thymidine (300 g/ml) for 17 to 19 hr, followed by BrdU treatment (30 g/ml) for 6.5 hr. Prior to harvest, they are exposed to ethidium bromide (7.5 g/ml) for 1.5 hr and Colcemid (0.02 g/ml) for 30 min. Using this method, high-resolution R-banded chromosomes at the 550–850 band level were obtained with frequencies at high as 70% of all mitotic cells. 相似文献
999.
Feran Agachan Jae Sik Joo Eric G. Weiss Steven D. Wexner 《Diseases of the colon and rectum》1996,39(10):S14-S19
PURPOSE: The aim of this study was to assess various intraoperative and postoperative complications associated with laparoscopic colorectal surgery. Specifically, the impact of surgical experience and procedure type on complications was analyzed. METHODS: All patients who underwent laparoscopic surgery were analyzed by age, sex, surgical indications, procedure performed, procedure length, intraoperative and postoperative complications, incidence and causes for conversion, duration of postoperative ileus, and length of hospital stay. Patients were classified for type of procedure and chronologically into four consecutive groups. Procedures were also categorized into four different groups: GI, total abdominal colectomies; GII, segmental resections; GIII, diverting procedures; GIV, others (abdominoperineal resection, Hartmann's creation or closure, anterior resection, and rectopexy). RESULTS: Between August 1991 and October 1995, 167 patients of a mean age of 49.6 (15–88) years underwent laparoscopic colorectal procedures. All procedures were electively performed. Common indications for surgery included inflammatory disease in 70 (42 percent), neoplasia in 56 (33 percent), functional bowel disorders in 30 (18 percent), and other forms of colorectal disorders in 11 (7 percent) patients. The most significant variable affecting intraoperative laparoscopic complication rate was surgical experience measured as the time interval during which surgery was performed (P=0.02). Total complication rate decreased from 29 percent during the first period to 11 percent by the second period (P<0.04) and 7 percent during the third period (P<0.005). Thus, the learning curve appeared to have required more than 50 cases to achieve. Moreover, even after performance of 94 (1991–1993) procedures in GI and GIV, these procedures were associated with higher complication rates than were those procedures in GII and GIII (P=0.04). CONCLUSION: Surgical experience and case selection are the most critical variables by which the surgeon can decrease the intraoperative laparoscopic complication rate. 相似文献
1000.
Morris E. FranklinJr. Daniel Rosenthal Daniel Abrego-Medina James P. Dorman Jeffrey L. Glass Richard Norem Antonio Diaz 《Diseases of the colon and rectum》1996,39(10):S35-S46
Laparoscopy for colonic diseases began in 1990 and has established a role in benign disease. Early observations and experiences demonstrated feasibility of laparoscopic surgery for a variety of colonic disease processes, but the applicability to colonic carcinoma was unclear. METHODS: In 1990, we began a comparative study of open (OCR)vs.laparoscopic (LCR) approach to colon cancer. The study progressed 65 months, with 224 patients in OCR group and 191 patients in LCR group. Parameters studied are stage, location, length of specimen, number of lymph nodes resected, margins, postoperative course, wound complications, recurrence rates, and immediate and long-term survival. OCR were standardized by one group, and LCR were standardized by a second group. All patients undergoing LCR were given freedom to choose either OCR or LCR, and informed consent was obtained. RESULTS: Equal or greater lymph node retrieval, resections, and distal margins were evident with LCR. Benefits with LCR were shown with shorter hospitalization (5.7vs.9.7 days), less blood loss, less wound problems (1vs.14), and quicker return of bowel function. Survival, recurrence, and death rates were essentially the same. There were no trocar implants in the LCR group. CONCLUSION: After five years, this study shows that laparoscopy does no harm to the patient, offers comparable oncologic resections, and seems to be patient-friendly, with less pain, quicker return of bowel functions, shortened hospitalization, and quicker return to full activity. 相似文献