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101.
Zhi-Qin Mao Mei Sun Department of Pediatrics Second Affiliated Hospital China Medical University Shenyang Liaoning Province China Ying Huang Hong Gao Department of Pediatric Surgery Second Affiliated Hospital China Medical University Shenyang Liaoning Province China Qiang Ruan Rong He Ying Qi Yu-Jing Huang Yan-Ping Ma Yao-Hua Ji Zheng-Rong Sun Virus Laboratory Second Affiliated Hospital China Medical University Shenyang Liaoning Province China 《World journal of gastroenterology : WJG》2007,(32)
AIM:To explore the genetic diversities of UL144 open reading frame (ORF) of cytomegalovirus DNA detected in colon tissue from infants with Hirschsprung's disease (HD) by sequencing UL144 DNA in 23 aganglionic colon tissue and 4 urine samples from 25 HD infants. METHODS:Nest PCR was performed for amplification of the UL144 gene. The UL144 gene was analyzed with softwares,such as DNAclub,BioEdit,PROSITE database,and DNAstar. RESULTS:The strains from HD patients were distributed among three genotypes of UL144:group 1A (64%),group 2 (24%),and group 3 (12%). The UL144 genotypes between strains from HD and control group were compared by chi square test (χ2 = 1.870,P = 0.393). Strains from the colon were sporadically distributed in UL144 genotypes. CONCLUSION:There are genetic diversities of UL144 ORF in colon tissue of infants with HD. However,cytomegalovirus UL144 genotypes are not associated with clinical manifestations of HD. 相似文献
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Q. Mao P. I. Terasaki J. Cai K. Briley P. Catrou C. Haisch L. Rebellato 《American journal of transplantation》2007,7(4):864-871
Longitudinal studies were conducted over a five-year period for HLA antibodies on 493 sera tested from 54 kidney transplant patients. HLA single antigen beads were employed to establish donor specificity of the antibodies. Only 3 of 22 patients without antibodies rejected a graft in contrast to 17 out of 32 patients with posttransplant antibodies (p = 0.003). Using a serum creatinine value of 4.0 mg/dL as the cut-off for a failed graft, 4 of 22 patients without antibodies failed compared to 21 of 32 with antibodies (p = 0.0006). Among patients with donor-specific antibodies (DSA) 13 of 15 failed (p = 0.000004). Even among patients with non-donor specific antibodies (NDSA), 8 of 17 failed (p = 0.05). Among patients who could be identified as making de novo antibodies (since they developed antibodies while not having antibodies for more than six months after transplantation), 6 of 11 failed (p = 0.03). Sequential testing for HLA antibodies shows that antibodies appear prior to a rise in serum creatinine and subsequent graft failure. The very strong association between the production of HLA antibodies after transplantation and graft failure indicates the importance of monitoring for posttransplant HLA antibodies. 相似文献
104.
阿弗菌素产生菌SIPI-AV-99081的选育 总被引:1,自引:0,他引:1
阿弗菌素链霉菌SIPI-AV-99081采用UV和NTG进行诱变处理。选育得到一高产菌株和几个典型的突变株。高产菌株AV-h-360的阿弗菌素B1的生产能力较出发菌株提高了3倍,AV-m-486和AV-m-796为阿弗菌素合成阻断突变株,它们都不能产生天然的阿弗菌素。 相似文献
105.
Wei Han Ming-Xing Xie Qing Lv Xin-Fang Wang Li Zhang 《Frontiers of Medicine in China》2010,4(1):71-76
The recent development of 2-dimensional strain (2D strain) imaging can provide a powerful alternative for assessing left ventricular
(LV) torsion. This study was conducted to evaluate the global and regional left ventricular twist by 2D strain in patients
with anterior wall myocardial infarction (AMI). A total of 55 AMI patients were divided into two groups according to their
ejection fraction (EF) values (group A: LVEF ⩾ 50%; group B: LVEF < 50%), and 35 normal people served as the control group.
Using 2-dimensional strain software, global and regional LV rotation and displacement were obtained at two planes. Compared
with the control group, patients of group A showed reduced peak LV twist of the anterior and anterior-septal wall (9.26±1.89
vs 10.74±2.67; 9.71±1.71 vs 11.36±2.29, both P < 0.05), but the radial displacement and global twist were maintained (P > 0.05). Differently, regional and global LV twist and radial displacement in patients of group B deceased significantly,
especially in the anterior and anterior-septal wall, as compared with patients in the control or group A (both P < 0.05). Moreover, a strong correlation was noted between peak twist and radial displacement; the twist-displacement loop
was markedly distorted in patients of group B. This study demonstrated that 2D strain has a potential ability for quantification
of left ventricular global and segment twist and radial displacement in patients with coronary artery disease. 相似文献
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109.
用下睑眼轮萜肌蒂瓣与鼻唇沟皮瓣一次性修复下睑全层缺损三例。男性1例,女性2例,平均年龄50岁,均为下睑1/2 ̄1/3全层缺损。基底细胞癌、睑板腺癌、鳞状细胞癌各1例,皮瓣全部成活。术后随访1年,下睑外形良好,闭眼功能正常。 相似文献
110.
作者对膝关节痛和休息痛病人21例做了股、胫骨内压和关节镜检查,其均值股骨髁0.143±0.01kPa,16例(76.19%)超过对照组0.130kPa(P<0.01);胫骨髁0.180±0.022kPa,18例(85.7%),超过对照组0.105kPa。同时测量膝关节腔内压21例。均值为0.135±0.035kPa,18例(85.7%)高于对照组0.120kPa。认为骨内压增高是引起膝关节痛的原因。本文还就膝关节特发性骨内高压症的临床表现、镜下特点、个体临界骨内压和治疗方法进行了探讨。 相似文献