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101.
102.
G. A. Mandell 《Pediatric radiology》1978,7(3):178-179
This case demonstrates that, as part of a favourable response to therapy, the ivory vertebrae of Hodgkin's disease can revert to a normal radiographic appearance. 相似文献
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Tom Walsh Ming K. Lee Silvia Casadei Anne M. Thornton Sunday M. Stray Christopher Pennil Alex S. Nord Jessica B. Mandell Elizabeth M. Swisher Mary-Claire King 《Proceedings of the National Academy of Sciences of the United States of America》2010,107(28):12629-12633
Inherited loss-of-function mutations in the tumor suppressor genes BRCA1, BRCA2, and multiple other genes predispose to high risks of breast and/or ovarian cancer. Cancer-associated inherited mutations in these genes are collectively quite common, but individually rare or even private. Genetic testing for BRCA1 and BRCA2 mutations has become an integral part of clinical practice, but testing is generally limited to these two genes and to women with severe family histories of breast or ovarian cancer. To determine whether massively parallel, “next-generation” sequencing would enable accurate, thorough, and cost-effective identification of inherited mutations for breast and ovarian cancer, we developed a genomic assay to capture, sequence, and detect all mutations in 21 genes, including BRCA1 and BRCA2, with inherited mutations that predispose to breast or ovarian cancer. Constitutional genomic DNA from subjects with known inherited mutations, ranging in size from 1 to >100,000 bp, was hybridized to custom oligonucleotides and then sequenced using a genome analyzer. Analysis was carried out blind to the mutation in each sample. Average coverage was >1200 reads per base pair. After filtering sequences for quality and number of reads, all single-nucleotide substitutions, small insertion and deletion mutations, and large genomic duplications and deletions were detected. There were zero false-positive calls of nonsense mutations, frameshift mutations, or genomic rearrangements for any gene in any of the test samples. This approach enables widespread genetic testing and personalized risk assessment for breast and ovarian cancer. 相似文献
105.
本文研制了以四苯硼—唐松草新碱缔合物为电活性物质的变价态唐松草新碱—PVC膜电极。电极膜按电活性物质:PVC:DBP为1:8:8组成。该电极在pH 5.0~6.0,Ⅰ=0.05的NaCl—HCl溶液中Nernst响应范围为1×10-3~1×10-5mol/L。电极斜率为58.2 mV/logc。检测限为2.5×10-6mol/L。用直接电位法考察了TDH+,TDH2CF++共存时溶液pH和电极斜率S的关系。用S—pH关系,测定了25℃,Ⅰ=0.05时的Ka1值为(2.5±0.2)×10-4,用E—pH关系,测定了25℃,Ⅰ=0.05时的Ka2值为(8.1±0.9)×10-8。 相似文献
106.
Chang Hoon Lee Hesham F. Nawar Lorrie Mandell Shuang Liang George Hajishengallis Terry D. Connell 《Vaccine》2010
The potent mucosal adjuvant properties of the type II heat-labile enterotoxin LT-IIa of Escherichia coli are dependent upon binding of the B pentamer of the enterotoxin (LT-IIa-B5) to ganglioside receptors on immunocompetent cells. To evaluate the immunomodulatory activities of LT-IIa-B5, in vitro experiments employing bone marrow-derived dendritic cells (BMDC) were performed. Uptake of OVA-FITC, a model antigen (Ag), was enhanced by treatment of BMDC with LT-IIa-B5, but not by treatment of cells with the B pentamer of cholera toxin (CTB). Expression of co-stimulatory molecules (CD40, CD80, CD86, and MHC-II) and cytokines (IL-12p40, TNF-α, and IFN-γ) was increased in BMDC treated with LT-IIa-B5. The capacity of LT-IIa-B5 to enhance Ag uptake and to induce expression of co-stimulatory receptors and cytokines by BMDC was dependent upon expression of TLR2 by the cell. Increased Ag uptake induced by LT-IIa-B5 was correlated with increased Ag-specific proliferation of CD4+ T cells in an in vitro syngeneic DO11.10 CD4+ T cell proliferation assay. These experiments confirm that LT-IIa-B5 exhibits potent immunomodulatory properties which may be exploitable as a non-toxic mucosal adjuvant. 相似文献
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Chlorozotocin, an anti-tumour agent lacking bone marrow toxicity at therapeutic doses: effects on lymphocyte subpopulations in mice. 下载免费PDF全文
R I Fisher G L Mandell F Bostick M G McMenamin T Anderson 《Clinical and experimental immunology》1980,39(2):416-425
Chlorozotocin is a new nitrosourea anti-tumour drug that does not produce bone marrow suppression at therapeutic doses in mice. CDF1 mice which were injected i.p. with a dose lethal to 10% of animals within 60 days (LD10), 20 mg/kg, developed a 50% reduction in circulating peripheral blood lymphocytes without a decrease in circulating granulocytes by day 3. Spleen weight also decreased markedly. The percentage of spleen B and T cells, determined by immunofluorescence with goat anti-mouse IgG and rabbit anti-mouse brain antisera, did not differ in control and chlorozotocin-treated mice. However, the ability of residual spleen cells to proliferate in response to phytohaemagglutinin, concanavalin A, pokeweed mitogen, and allogeneic cells was significantly suppressed although the lipopolysaccharide response was not reduced. The ability of the mice to respond to a primary immunization with sheep red blood cells was not significantly impaired. Therefore, chlorozotocin has a cytotoxic effect on both B and T cells but selectively inhibits the proliferative capacity of T cells. B cell proliferation and B cell function as measured in a primary antibody response were not reduced. These studies suggest chlorozotocin may be useful as an immunosuppressive drug as well as an anti-tumour agent. 相似文献
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