人参皂苷Rg1对大脑皮质神经细胞生物膜的影响

目的:研究人参皂苷Rg1对原代培养正常大鼠大脑皮质神经细胞生物膜的影响。方法:采用细胞培养技术,运用比色法、硫代巴比妥酸法、黄嘌呤氧化酶法(羟胺法)和微量酶标法测定人参皂苷Rg1对细胞内ACP活力、MDA含量、SOD活力及LDH释放量的影响。结果:1mg/ml、2 mg/ml及4 mg/ml人参皂苷Rg1作用30min可不同程度增加正常培养神经细胞的LDH释放量和细胞内SOD活力,降低细胞内ACP活力及MDA含量。结论:1mg/ml、2 mg/ml及4 mg/ml人参皂苷Rg1作用30min对正常培养大脑皮质神经细胞生物膜具有显著影响,其中对溶酶体膜有保护作用,对生物膜的脂质过氧化反应有抑制作用,能减轻自由基对生物膜的攻击及损伤,但对细胞膜可能有一定的损伤作用。     

胆囊结石术后胃肠功能紊乱的针刺研究

目的:观察不同针刺刺激量对胆囊结石术后胃肠功能紊乱患者胃肠功能的恢复以及血管活性肠肽(VIP)的影响。方法:将32例患者分为四组,分为提插强刺激组、提插弱刺激组、捻转强刺激组、捻转弱刺激组,观察给予不同针刺刺激后四组患者的胃肠功能和血管活性肠肽(VIP)的变化。结果:统计学处理后,对于肠鸣音恢复时间,提插强刺激组和捻转弱刺激组之间的比较有统计学意义;对于术后排气时间,提插强刺激与捻转强刺激、捻转弱刺激之间,提插弱刺激与捻转弱刺激之间均有统计学意义(P<0.05),其余无统计学意义;术后排便时间统计结果无差异(P>0.05)。对于VIP,四组病人术前血管活性肠肽无差异(P>0.05),具有可比性;术后第2天四组病人血浆血管活性肠肽含量治疗后均有回升趋势,提插弱刺激组和捻转弱刺激组病人血浆VIP含量在治疗后与提插强刺激组和捻转强刺激组比较均有明显差异(P<0.05)。结论:强刺激对血管活性肠肽的变化的影响较弱刺激好,同样的刺激量下提插较捻转的对血管活性肠肽的变化影响大,提插强刺激对于胃肠功能恢复的作用由于其他三种。     

Exploring the mechanism of Buyang Huanwu decoction in the treatment of lumbar disc herniation based on network pharmacology and molecular docking

Buyang Huanwu decoction (BYHWD), as one of the traditional Chinese medicine formulas, is widely used in the clinical treatment of lumbar disc herniation (LDH) with curative effect. It has the characteristics of multi-component, multi-target, and mutual synergy, but the mechanism of action is often unclear. It needs some research to explore the molecular mechanism of BYHWD in the treatment of LDH based on network pharmacology and molecular docking. Screen the active compounds of BYHWD and predict drug-related gene/protein targets, which could determine the specific target of BYHWD in the treatment of LDH. Construct the “Drugs-Compounds-Targets” network and search for the core targets. Use Gene Ontology functional enrichment analysis, Kyoto Encyclopedia of Genes and Genomes pathway enrichment analysis, and molecular docking verification to explore the possible molecular mechanism. Eighty-two effective compounds and 666 targets of BYHWD, 187 targets for LDH treatment, and 20 core candidate targets were excavated. A total of 3414 entries were identified by Gene Ontology enrichment analysis, 173 related signal pathways were identified by Kyoto Encyclopedia of Genes and Genomes enrichment analysis, and 5 core compounds were identified by molecular docking, which had a good affinity with core genes STAT3, JUN, AKT1, MAPK1, RELA, and PIK3CA. BYHWD may play the role of analgesic and improving function by synergistic anti-inflammatory and analgesic compounds, regulating cell metabolic differentiation, regulating immunity, and anticoagulation. BYHWD in the treatment of LDH may play a role in analgesia and improve function through multiple signaling pathways, including PI3K-Akt, mitogen-activated protein kinase, tumor necrosis factor, and interleukin-17. The PI3K-Akt signaling may be one of the key mechanisms.     

菟丝子定向诱导成骨细胞向神经元细胞转化的研究

目的：开展菟丝子醇提物诱导大鼠成骨细胞向神经元细胞转化的定向诱导研究．方法：采用菟丝子醇提物刺激成骨细胞的分化，用RT-PCR检测和倒置显微镜下观察菟丝子水提物对成骨细胞刺激后的变化。结果：RT-PCR检测有神经元细胞和神经胶质细胞的特异性蛋白神经元烯醇酶（NSE）和胶质纤维酸性蛋白（GFAP）表达，在倒置显微镜下观察到神经元样细胞生长．结论：在体外，菟丝子醇提物具有诱导大鼠成骨细胞向神经元细胞定向分化的作用。     

VVS患者心率变异性、CK、CK-MB变化及治疗前后变化的意义

目的：探讨血管迷走性晕厥(VVS)患者的心率变异性指标、血清肌酸激酶(CK)、肌酸激酶同工酶(CK-MB)的变化及其临床意义。方法：选取我院心血管内科收治的VVS患者67例作为VVS组、领选取70例同龄健康体检对象作为对照组;对比两组的心率、超声心动图参数、心率变异性指标及血清CK、CK-MB水平,并对比67例患者在接受射频导管消融术治疗前后的上述指标变化情况。结果：VVS组患者的最大心率与对照组差异无统计学意义;VVS组患者的最慢心率、平均心率均低于对照组;VVS组的心率总体标准差、差值标准差、差值均方根均高于对照组;VVS组患者的左心房前后径、左心室舒张末内径、左心室射血分数与对照组差异无统计学意义;VVS组患者的血清CK、CK-MB均低于对照组;经射频导管消融术治疗后,VVS组患者的平均心率较治疗前提高;VVS组的心率总体标准差、差值标准差、差值均方根均较治疗前降低;经射频导管消融术治疗后,VVS组血清CK、CK-MB均较治疗前提高。结论：VVS患者的心率变异性更加明显、血清CK、CK-MB水平降低,采用射频导管消融术治疗能显著改善患者的心率变异性。     

SUMOylation of Kir7.1 participates in neuropathic pain through regulating its membrane expression in spinal cord neurons

AimsPotassium (K⁺) channels have been demonstrated to play a prominent involvement in nociceptive processing. Kir7.1, the newest members of the Kir channel family, has not been extensively studied in the CNS, and its function remains largely unknown. The present study investigated the role of spinal Kir7.1 in the development of pathological pain.Methods and ResultsNeuropathic pain was induced by spared nerve injury (SNI). The mechanical sensitivity was assessed by von Frey test. Immunofluorescence staining assay revealed that Kir7.1 was predominantly expressed in spinal neurons but not astrocytes or microglia in normal rats. Western blot results showed that SNI markedly decreased the total and membrane expression of Kir7.1 in the spinal dorsal horn accompanied by mechanical hypersensitivity. Blocking Kir7.1 with the specific antagonist ML418 or knockdown kir7.1 by siRNA led to mechanical allodynia. Co‐IP results showed that the spinal kir7.1 channels were decorated by SUMO‐1 but not SUMO‐2/3, and Kir7.1 SUMOylation was upregulated following SNI. Moreover, inhibited SUMOylation by GA (E1 inhibitor) or 2‐D08 (UBC9 inhibitor) can increase the spinal surface Kir7.1 expression.ConclusionSUMOylation of the Kir7.1 in the spinal cord might contribute to the development of SNI‐induced mechanical allodynia by decreasing the Kir7.1 surface expression in rats.     

Combined prognostic value of preoperative serum thyrotrophin and thyroid hormone concentration in papillary thyroid cancer

BackgroundA growing number of studies have found a close association between thyroid hormones and thyrotrophin (TSH), and they also have prognostic significance in some cancer types; this study aimed to investigate the prognostic value of free triiodothyronine (fT3), free thyroxine (fT4), fT3/fT4, TSH, and their combination in patients with papillary thyroid carcinoma (PTC).MethodsThis study retrospectively analyzed the relevant data of 726 newly diagnosed PTC patients. Both univariate and multivariate analyses were used to predict the recurrence rate, and a risk score was established. In addition, with the use of a random survival forest, a random forest (RF) score was constructed. After calculating the area under the curve (AUC), the diagnostic efficacy of risk score, RF score, and four indicators was compared.ResultsfT3, fT4, fT3/fT4, and TSH were strongly associated with some invasive clinicopathological features and postoperative recurrence. Patients with high expression of fT4 and TSH have a high risk of recurrence. By contrast, patients with high expression of fT3 and fT3/fT4 have a low risk of recurrence. At the same time, the combined use of various indicators is more helpful for establishing an accurate diagnosis. By comparison, we found that the RF score was better than the risk score in terms of predicting the recurrence of PTC.ConclusionThe diagnostic accuracy of a combination of fT3, fT4, fT3/fT4, and TSH can help improve our clinical estimate of the risk of recurrent PTC, thus allowing the development of a more effective treatment plan for patients.     

Preparation and photodynamic antibacterial/anticancer effects of ultralong-lifetime room-temperature phosphorescent N-doped carbon dots

Room-temperature phosphorescent (RTP) N-doped carbon-dots (CNDs) featuring eco-friendliness, low cost and high biocompatibility, are ideal photodynamic antibacterial and anticancer nanomaterials. However, the existing CNDs are limited by low singlet oxygen (¹O₂) quantum yield, which has become a bottleneck in the development of CNDs. One basic reason is the short T₁-state exciton lifetime of CNDs. Herein, triethylenetetramine hexaacetic acid was used to synthesize CNDs via a one-step hydrothermal method. CNDs are characterized with low toxicity, high biocompatibility and ultralong-lifetime RTP (URTP). In addition to the URTP (average lifetime 414 ms) under solid conditions, CNDs even had URTP (average lifetime 320 ms) in a water environment. The ultralong T₁ exciton lifetime largely extends the collision time between T₁ state excitons and O₂ and prolongs the energy transfer time, not only improving the quantum yield (0.63) of singlet oxygen (¹O₂) in solution, but also facilitating the photodynamic antibacterial and anticancer effects.

The URTP CNDs largely extends the collision time between T₁ state exciton and O₂, which improve quantum yield of singlet oxygen (¹O₂) in solutions, facilitating the photodynamic antibacterial and anticancer effects.     

乳猪肝制备肝水解肽的工艺研究

目的以乳猪肝为原料制备肝水解肽，分析产品活性。方法应用酶水解／超滤法提纯制备肝水解肽，采用Folin-酚法、四氮甲唑蓝（MTT）法测定含量及活性，并与成年动物肝脏对比。结果乳猪肝组收率明显高于成年动物肝组，产品活性与后者的比较，极显著性差异。结论采用乳猪肝制备肝水解肽，产品的生物活性高，收率好，具有一定的应用价值？     

非小细胞肺癌survivin和Flk1表达及关系分析

目的:观察survivin蛋白和血管内皮细胞生长因子(VEGF)受体FIk1在不同病理类型及不同临床分期的非小细胞肺癌(nonsmall-cell lung cancer,NSCLC)中的表达规律并探讨其相互关系。方法:采用临床NSCLC患者病理标本进行研究,NSCLC组为56例非小细胞肺癌肿瘤病理标本,患者临床资料分类中,临床Ⅰ期15例、Ⅱ期28例、Ⅲ期13例,病理分级分类中高分化肿瘤16例、中分化肿瘤22例、低分化肿瘤18例,对照组为18例癌旁组织。采用SABC法进行survivin蛋白和FIk1蛋白表达的免疫组织化学检测,分析染色结果确定阳性和阴性。结果:56例NSCLC中44例survivin表达阳性,survivin阳性率为78.6%(44/56),显著高于癌旁组。survivin阳性表达在不同NSCLC肿瘤病理分级中相差显著,在不同临床分期、不同淋巴结转移中显著不同。56例NSCLC中42例FIk1表达阳性,占75.0%(42/56),显著高于癌旁组。FIk1阳性表达在不同NSCLC肿瘤病理分级中相差显著,在不同临床分期、不同淋巴结转移中显著不同。NSCLC中survivin与FIk1阳性表达显著相关。结论:Survivin和FIk1共同高表达与NSCLC发生发展及浸润转移有显著关系。