人体运动信息及其在国内科学训练中的应用

本文论述了人体运动信息的构成,获得途径及其在国内科学训练中的指导作用。     

眼科超声乳化系统中注吸技术的研究

目的介绍白内障超声乳化仪中注吸系统的特点及设计方案,重点探讨当今超声乳化技术应用高负压、低能量的特点带来的前房涌动问题及解决办法.方法研究分析前房涌动时抽吸管路中负压变化模式,对负压进行实时监测,一旦出现符合前房涌动的变化模式,立即将抽吸管路中的压力释放,瞬间消除负压,使前房涌动程度控制在初始状态,有效防止手术中前房塌陷.结果采用抽吸平稳的六轮蠕动泵,负压传感器与微机相连,浪涌模式一旦发生,高速电磁阀可在100ms内控制释放压力,达到瞬间消除负压的目的.结论实验证明,采用涌动抑制技术的测试帽仅有微小塌陷,并能在0.5 s以内恢复常态;而未采用涌动抑制时的测试帽塌陷严重,需2～3 s方能恢复.产品经国家有关部门检测,各项技术指标符合要求;应用结果证明,采用防前房涌动抑制技术后,前房稳定性明显提高.     

Modified Ureterosigmoidostomy

Objective To introduce an operation procedure and evaluate the coutinence diversion results of the modified ureterosigmoidostomy after radical cystectomy. Methods Fourteen cases of bladder cancer or prostate carcinoma were operated on with modified Sigma pouch from Feb, 1998 to Dec, 1999. A longitudinal incision about 25 cm on the sigmoid uall was done to form a low pressure pouch. The vertex of the new pouch was fixed to sacrum. Both ends of ureters were anastomosed side to side and to form a big nipple and inserted into the top of pouch for 2 to 3 centimeters. Results It took about sixty five minutes to create a new low pressure pouch after radical cystectomy. Early complication of was found in two cases postoperatively, and cured with temporary colonostomy. Hydronephrosis and hypokalemia in one patient were cured by percutaneous anterograde ureter dilatation with balloon and oral replacement of potassium salt. A~ patients displayed urinary continence. No symptomatic renal infection or hypercholoraemic acidosis occurred. Conclusion Modified ureterosigmoidostomy is a safe procedure of urinary diversion and provides a big volume, low intravesical pressure pouch. The patients are free from the troublesome urine-bag, intermittert catheterization , and upper urinary tracts are protected effectively. The quality of life is satisfied.     

结核杆菌热休克蛋白70基因的克隆与原核表达

目的：克隆结核杆菌热休克蛋白70(TBhsp70)基因，并在大肠杆菌中表达。方法：利用PCR技术从结核杆菌H37Rv中扩增Hsp70基因，并将其克隆到pUC19中，进行测序。将得到的Hsp70基因克隆到表达载体pGEX-4T-1中，构建重组表达质粒pGEX—TBhsp70，在大肠杆菌DH5α中进行表达。结果：成功地克隆了TBhsp70基因。DNA测序证实，与GenBank公布的序列一致。含pGEX-TBhsp70基因表达质粒的大肠杆菌经IPTG诱导后，能够表达相对分子质量(MR)约为96000的融合蛋白。结论：获得了TBhsp70基因，成功地构建了原核表达质粒pGEX-TBhsp70，并在大肠杆菌得到表达，为其相关研究奠定了一定的基础。     

力尔凡对晚期非小细胞肺癌化疗的影响

比较全身化疗加或不加力尔凡对晚期非小细胞肺癌(NSCLC)的疗效、不良反应及免疫功能的变化。方法:将60例晚期NSCLC患者以随机方法(开信封)分别分为并用力尔凡组(治疗组)和单用化疗组(对照组),分别接受NP方案(诺维苯、顺铂)加力尔凡或单用NP方案化疗。结果:1)治疗组部分缓解率43.3%,对照组33.3%(P>0.05),中位缓解期分别为4.8个月及3.3个月。2)治疗组疗后和疗前相比CD4及CD4/CD8值显著升高(P<0.05),而对照组则相反,两组差异显著(P<0.05)。3)不良反应:治疗组白细胞下降63.3%,对照组达100%(P<0.05)。结论:力尔凡与化疗同时应用治疗晚期NSCLC,疗效有所提高,可增强细胞的免疫功能,对化疗引起的白细胞下降有保护作用。     

In situ growth of CuS NPs on 3D porous cellulose macrospheres as recyclable biocatalysts for organic dye degradation

Aiming at recyclable catalyst carriers, porous cellulose macrospheres from wood pulp dissolved in an alkaline urea system were regenerated by simple injection regeneration. After solvent exchange, porous cellulose macrospheres (CMs) with a high specific surface area of 325.3 m² g⁻¹ were obtained by lyophilization, and CuS nanoparticles (CuS NPs) were coated on CMs by in situ growth in the liquid phase to achieve CuS-supported CM macrospheres (CuS@CM). The results indicated that the CuS@CM biocatalyst was successfully prepared with an average diameter of approximately 1.2 mm. In addition, CuS@CM was further used as a heterogeneous catalyst for the catalytic degradation of methylene blue (MB) and methyl orange (MO) model dyes during the oxidation of hydrogen peroxide (H₂O₂). In the presence of low doses of H₂O₂, the degradation rate of MB reached 94.8% within 10 min, showing high catalytic activity under neutral and alkaline conditions. After five cycles, 90.1% of the original catalytic activity was still retained, indicating that the prepared CuS@CM composite possessed excellent catalytic activity and reusability.

CuS nanoparticles were grown in situ on 3D porous cellulose macrospheres for an excellent rapid cycling removal of organic dyes.     

Bayesian反馈法估算体外循环术后患儿静注头孢拉定的药动学参数

目的：用Ｂａｙｅｓｉａｎ 反馈法估算头孢拉定在体外循环术后患儿的药动学参数,为临床应用提供依据。方法：采用ＨＰＬＣ法测定头孢拉定血药浓度。选择不同取样时间的１ 个血药浓度作为反馈,用Ｂａｙｅｓｉａｎ 法估算个体药动学参数,并与经典药动学方法估算的结果比较。结果：二步法估算的群体药动学参数为,Ｔ１／２α　　０－３０ ±０－１０ｈ, Ｔ１／２β　　３－０８ ±１－６８ｈ, Ｖｃ ０－２４ ±－１５Ｌ·ｋｇ－ １, Ｖｄ １－０９ ±０－５７Ｌ·ｋｇ－１ , Ｃｌ２４８－３±５５－４ｍｌ·ｈ－１·ｋｇ－１ 。用１ 个血药浓度反馈估算的药动学参数与经典药动学方法估算结果之间的差异无显著性。结论：用Ｂａｙｅｓｉａｎ 反馈法估算个体药动学参数及预测血药浓度,可满足临床优化个体化给药方案的需要     

白血病细胞凋亡与bcl-2和bax-alpha表达的研究

目的　研究白血病细胞细胞凋亡率与 bcl- 2和bax- alpha阳性率及表达水平关系。方法　选择难治或复发的化疗耐药白血病患者和初治化疗敏感白血病患者各 10例 ,分离白血病细胞 ,柔红霉素 (5 0 0 ng/ ml)诱导凋亡 ,应用流式细胞术比较分析耐药组和敏感组白血病细胞的凋亡率与 bcl- 2和 bax- alpha阳性率和表达水平的关系。结果　化疗耐药组和化疗敏感组对柔红霉素诱导的凋亡率有显著差异 ,敏感组 (2 3.2 6 %± 12 .94% )显著高于耐药组 (2 .2 6 %± 1.44 % ) ,(P=0 .0 0 1) ;耐药组白血病细胞凋亡率与 bcl- 2阳性率呈负相关 (r=- 0 .6 47,P=0 .0 43) ,与 bax- alpha阳性率和相对荧光强度呈正相关 (r=0 .883,P=0 .0 0 1;r=0 .814,P=0 .0 0 4)及 bcl- 2 :bax- alpha比率呈负相关 (r=-0 .893,P=0 .0 0 1) ;敏感组凋亡率 bax- alpha的阳性率和荧光强度呈正相关 (r=0 .6 5 3,P=0 .0 41,r=0 .76 2 ,P=0 .0 10 ) ,与 bcl- 2 :bax- alpha比率呈负相关 (r=- 0 .741,P=0 .0 14)。结论　白血病细胞耐药性与细胞的凋亡抗性有关 ,这种凋亡抗性与 bcl- 2和 bax- alpha的表达比例增高有关 ,特别是 bax- alpha的表达水平     

儿童髋关节一过性滑膜炎的B超诊断

目的 :探讨 B超对儿童髋关节一过性滑膜炎 (TSH)的检查诊断标准。方法 :选取确诊的单侧病变 6 6例 ,采用髋关节前侧切面 ,检查股骨颈颈前间隙 ,并随机抽查 77例健康儿童双髋对照。结果 :TSH的患髋股骨颈颈前间隙增宽 ,统计学处理有显著意义 (P<0 .0 0 1)。 TSH超声声像分为关节囊肿胀型与关节腔积液型。结论 :超声声像能够准确反映正常髋关节组织结构和 TSH病变变化 ,可客观诊断该病