早期静脉注射葡萄糖酸钙对间斑寇蛛毒致痛大鼠行为学的影响

目的观察预先静脉注射葡萄糖酸钙溶液,对问斑寇蛛毒致痛大鼠行为学的影响;评估葡萄糖酸钙溶液对抑制问斑寇蛛毒致痛作用的疗效。方法将雄性SD大鼠80只随机分为葡萄糖酸钙治疗组与致痛组,治疗组大鼠预先尾部静脉注射葡萄糖酸钙溶液,两组大鼠随机分为5组,分别以低剂量（10〉L／kg）、中低剂量（40μL／kg）、中剂量（100μL／kg）、高剂量（300μL／kg）的间斑寇蛛毒素及福尔马林（10％甲醛溶液）进行足底注射,观察记录两组大鼠注射后2、15、30min及2h缩足、舔足次数。结果（1）中低剂量组和高剂量组的蛛毒致痛大鼠缩足次数在15min内均高于福尔马林致痛组,然而30rain却低于福尔马林致痛组（P〈0．05）;（2）15min时致痛组及治疗组大鼠缩足次数均与毒素浓度呈显著的正相关（r^2=0．597,P〈0．05;r^2=0．426,P〈0．05）;（3）葡萄糖酸钙治疗组与各毒素致痛组相比,大鼠缩足次数均减少（P〈O．05）。结论间斑寇蛛毒素致痛大鼠的作用相对于福尔马林在30min内更为强烈,但是30min后则较福尔马林组有所减轻,预先静脉注射葡萄糖酸钙的方法对间斑寇蛛毒致痛有明显的镇痛作用。     

四肢关节结核的X线及CT诊断

目的:探讨四肢关节结核的X线平片和CT表现,评价X线平片和CT在四肢关节结核的应用价值。材料与方法:对经手术或临床证实的24例四肢关节结核的影像学资料进行回顾性分析。所有患者均行X线平片检查,15例同时行CT扫描。结果:23例为单关节受累,1例为两个关节受累。8例发生在膝关节,7例发生在髋关节,3例发生在踝关节,2例发生在腕关节,2例发生在肘关节,发生在跖趾关节、肩关节各1例。表现为骨质破坏15例,表现为骨质疏松7例,仅表现为软组织肿胀而无骨异常改变者2例结论:X线平片在四肢关节结核中可作为初步筛选方法:,CT能提供更多的诊断信息,两者在四肢关节结核的诊断中起着不可或缺的作用。     

新疆维吾尔族妇女宫颈上皮内瘤变及宫颈癌中FHIT、P16~(INK4a)、RARβ蛋白的表达及意义

目的探讨脆性组氨酸三联体(fragile histidine triad,FHIT)、P16INK4a与视黄酸受体β(retinoic acid receptor-beta,RARβ)蛋白在新疆维吾尔族妇女宫颈上皮内瘤变(cervical intra-epithelial neoplasia,CIN)及宫颈癌中的表达及其意义。方法采用免疫组化链霉素抗生物素蛋白-过氧化物酶链接(SP)法检测20例慢性宫颈INK4a炎、30例CIN(CINⅠ、CINⅡ、CINⅢ各10例)以及40例浸润性宫颈鳞癌组织标本中FHIT、P16INK4a及RARβ蛋白的表达。结果(1)FHIT蛋白阳性表达率依次为慢性宫颈炎(90.00%)CIN(66.67%)浸润性宫颈鳞癌INK4a(27.50%)(P=0.000);P16和RARβ蛋白阳性表达率依次为浸润性宫颈鳞癌(82.50%;90.00%)CIN(46.67%;53.33%)慢性宫颈炎(0.00%;10.00%)(P=0.000;P=0.000)。(2)在宫颈各组织中,FHIT蛋白INK4a与P16INK4a蛋白表达呈负相关(r=-0.384,P=0.000);FHIT蛋白与RARβ蛋白表达呈负相关(r=-0.291,P=0.006);P16INK4a蛋白与RARβ蛋白表达呈正相关(r=0.445,P=0.000)。结论抑癌基因FHIT表达缺失与P16INK4a和RARβ过度表达与宫颈癌的发生发展密切相关,并且FHIT、P16INK4a、RARβ具有协同作用。FHIT蛋白与P16INK4a蛋白和RARβ蛋白的联合检测可作为宫颈癌早期诊断和宫颈癌进展的分子指标。     

甜高粱秸秆与酒糟青贮品质变化及饲喂效果初评

[目的]探索甜高粱酒糟作为青贮原料的可能性,并通过饲喂试验研究了甜高粱青贮的增重效果,以期为甜高粱酒糟饲料化利用提供理论依据.[方法]本试验以甜高粱秸秆和酒糟为青贮原料,通过感官得分、理化指标变化评价甜高粱青贮的品质,探讨青贮菌剂对甜高粱秸秆青贮过程中pH值、粗纤维、总酸、总糖、干物质等成分的影响,并结合饲喂试验,分析了甜高粱青贮饲喂绵羊的增重效果.[结果]结果表明,甜高粱酒糟的青贮品质略低于秸秆,等级为Ⅱ级尚好,具有作为青贮原料的潜力;添加菌剂组甜高粱秸秆的总糖和还原糖含量分别比对照组高了125%和239%,粗纤维比对照组降低了21.4%,青贮菌剂可以提高总酸含量、降低青贮pH值;从饲喂试验结果来看,利用甜高粱青贮饲喂绵羊可以得到较高的日均增重,其中6月龄绵羊的日均增重大于4月龄和2月龄.6月龄绵羊的日均增重为84 g,显著高于对照组.[结论]青贮菌剂可以提高甜高粱秸秆和酒糟的发酵品质、改善营养品质,延长秸秆和酒糟的贮存时间,甜高粱青贮是一种很好的动物饲料.     

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??OBJECTIVE To prepare the transferrin modified harmine magnetoliposomes and investigate its in vitro properties and antitumor effect. METHODS Transferrin modified harmine magnetoliposomes was prepared by reverse phase evaporation method. The size, Zeta potential, entrapment efficiency, drug loading efficiency, release rate and plasma stability of liposomes were investigated. Furthermore, cytotoxicity to liver cancer and glioma were investigated too. RESULTS The transferrin modified harmine magnetoliposomes were successfully prepared. The average size of liposomes was (184.7??8.08)nm.The average Zeta potential was (-12.4??0.896) mV and average entrapment efficiency was (84.67??6.26)%. Drug loading efficiency was (9.14??4.54)%. Transferrin modified harmine magnetoliposomes released more slowly and were more stable in rat plasma than harmine solution. The results of cytotoxicity test showed that the cell inhibitory effect of transferrin modified harmine magnetoliposomes was enhanced compared with other groups, and the cell inhibitory effect of this preparation was more obvious underwhen cells were put under magnetic field compared with those under the non-magnetic field(P<0.05). CONCLUSION This method can be successfully used to prepared transferrin modified harmine magnetoliposomes, and liposomes can inhibit the cell visbility of liver cancer and human glioma cells significantly.     

新疆和田地区内科医师支气管哮喘认知状况的调查分析

目的 了解新疆和田地区内科医师对支气管哮喘(简称哮喘)基本概念、治疗及管理的认知状况,为基层少数民族医师的培训提供依据.方法 对和田地区26家不同级别医院共246名内科医师进行有关哮喘相关知识问卷调查,其中二级甲等医院1家,内科医师68名;二级乙等医院7家,内科医师122名;一级医院18家,内科医师56名.问题涉及:哮喘定义及哮喘炎症本质、哮喘的症状和诊断、哮喘的药物治疗和首选治疗方法、哮喘防治中存在的误区、全球哮喘防治创议(GINA)知晓情况及医院是否备有吸入激素药物等.结果 对哮喘属气道慢性炎症及哮喘治疗方面如首选治疗方法、治疗目标及控制水平分级,回答正确率仅分别为11.8％、21.5％、8.5％和2.0％,且上述问题在二级与一级医院间差异有统计学意义(P＜0.05).对哮喘GINA知晓率、吸入方法的掌握及慢性疾病理念方面正确率分别为9.3％、13.8％和9.8％.二级甲等医院与二级乙等医院及一级乡镇医院相互间差异有统计学意义(P＜0.05).关于医院备有吸入支气管扩张剂或糖皮质激素方面,全地区七县一市共8家二级医院均备有药物(1家二级甲等地区医院、7家二级乙等县医院).一级乡镇医院均无短效支气管扩张剂和糖皮质激素吸入剂的备药.结论 我国边疆基层医院,尤其是新疆南疆一、二级县乡镇医院对哮喘的认知防治管理方面存在较多问题,知识陈旧,对GINA知晓率低,不利于基层大量哮喘患者的治疗及管理,规范培训基层少数民族医务人员,使其掌握哮喘防治指南,从而有效指导哮喘患者是基层哮喘管理亟待解决的问题.     

新发现的内脏利什曼病流行区新疆民丰县Ⅰ. 流行病学调查

目的 对新疆民丰县一个新发现的内脏利什曼病（黑热病）流行区进行现场流行病学调查。 方法 2007年6月采用回顾性调查和现况调查相结合,对民丰县安迪尔乡所有居民进行逐户入户调查。内容包括家庭成员中20年来的健康状况、既往有无疑似内脏利什曼病史（包括已过世者）,并对15岁以下儿童进行体查（触诊肝、脾）;对部分居民进行利什曼素皮内试验和rk39免疫层析试条检测。 结果 共调查居民313人,未见现症病例。既往有疑似内脏利什曼病史者60例,其中13例已过世。171人进行利什曼素皮内试验检测,阳性率为99.4%（170/171）,其中有疑似内脏利什曼病病史者的阳性率为96.6%（28/29）。197人进行rk39免疫层析试条检测,阳性率为10.2%（20/197）,其中有疑似内脏利什曼病史者的阳性率为19.4%（6/31）,追溯诊断为既往内脏利什曼病病例。 结论 新疆民丰县为内脏利什曼病流行区。     

Autophagy is involved in oral rAAV/Aβ vaccine-induced Aβ clearance in APP/PS1 transgenic mice

The imbalance between β-amyloid(Aβ) generation and clearance plays a fundamental role in the pathogenesis of Alzheimer 's disease(AD). The sporadic form of AD is characterized by an overall impairment in Aβ clearance. Immunotherapy targeting Aβ clearance is believed to be a promising approach and is under active clinical investigation. Autophagy is a conserved pathway for degrading abnormal protein aggregates and is crucial for Aβ clearance. We previously reported that oral vaccination with a recombinant AAV/Aβ vaccine increased the clearance of Aβ from the brain and improved cognitive ability in AD animal models, while the underlying mechanisms were not well understood. In this study, we first demonstrated that oral vaccination with rAAV/Aβ decreased the p62 level and up-regulated the LC3BII/LC3B-I ratio in APP/PS1 mouse brain, suggesting enhanced autophagy. Further, inhibition of the Akt/m TOR pathway may account for autophagy enhancement. We also found increased anti-Aβ antibodies in the sera of APP/PS1 mice with oralvaccination, accompanied by elevation of complement factors C1 q and C3 levels in the brain. Our results indicate that autophagy is closely involved in oral vaccination-induced Aβ clearance, and modulating the autophagy pathway may be an important strategy for AD prevention and intervention.     

LncRNA CASC15通过miR-153-3p/Nrf2反馈环调控胃癌细胞增殖、侵袭和上皮间质转化

目的:探讨LncRNA CASC15通过miR-153-3p/Nrf2反馈环对SGC-7901细胞增殖、侵袭和上皮间质转化(EMT)的影响.方法:采用实时荧光定量PCR(RT-qPCR)法分析胃癌组织、癌旁正常组织、SGC-7901细胞、GES-1细胞中LncRNA CASC15、miR-153-3p和Nrf2的表达量...