Further genetic evidence implicates the vasopressin system in childhood-onset mood disorders

Studies in both animals and humans advocate a role for the vasopressin (AVP) system in the aetiology of depressive symptoms. Attention has particularly focused on the role of AVP in the overactivation of the hypothalamic-pituitary-adrenal (HPA)-axis in mood disorders. Elevated AVP plasma levels have been found in mood disorder patients, which are often positively correlated with the severity of symptoms. We recently reported an association between childhood-onset mood disorders (COMD) and polymorphisms in the receptor responsible for the AVP-mediated activation of the HPA-axis ( AVPR1B ). As genetic variation in the vasopressinergic system could provide a mechanism to explain the endocrine alterations observed in mood disorders, we investigated other genes in this system. The gene encoding AVP is the strongest candidate, particularly as genetic variation in this gene in rodents is associated with anxiety-related behaviours. Six single-nucleotide polymorphisms (SNPs) were genotyped across the AVP gene in a sample comprised of 586 Hungarian nuclear families ascertained through affected probands with a diagnosis of COMD. In addition, AVP coding and putative regulatory regions were screened for mutations using denaturing high-performance liquid chromatography. One SNP, 3' to the AVP, gene reached significance ( P  =   0.03), as did the overtransmission of a five-marker haplotype with a frequency of 22% ( P  =   0.0001). The subsequent mutation screen failed to identify any putative functional polymorphisms. The outcome of this study, combined with our previous association between COMD and AVPR1B , implicates genetic variation in vasopressinergic genes in mediating vulnerability to COMD.     

Dynamic force spectroscopy of single DNA molecules

To explore the analytic relevance of unbinding force measurements between complementary DNA strands with an atomic force microscope, we measured the forces to mechanically separate a single DNA duplex under physiological conditions by pulling at the opposite 5'-ends as a function of the loading rate (dynamic force spectroscopy). We investigated DNA duplexes with 10, 20, and 30 base pairs with loading rates in the range of 16-4,000 pN/s. Depending on the loading rate and sequence length, the unbinding forces of single duplexes varied from 20 to 50 pN. These unbinding forces are found to scale with the logarithm of the loading rate, which is interpreted in terms of a single energy barrier along the mechanical separation path. The parameters describing the energy landscape, i.e. , the distance of the energy barrier to the minimum energy along the separation path and the logarithm of the thermal dissociation rate, are found to be proportional to the number of base pairs of the DNA duplex. These single molecule results allow a quantitative comparison with data from thermodynamic ensemble measurements and a discussion of the analytic applications of unbinding force measurements for DNA.     

Auditory event-related potentials in poor readers.

Although poor readers (PR) are considered the major group among reading-disabled children, there are not event-related potentials (ERP) studies reported of PR on the subject. In this study, attentional and memory processes were studied in an auditory oddball task in PR and normal controls. ERP to auditory stimuli were recorded in 19 leads of the 10/20 system, using linked earlobes as references, in 20 normal children (10 female) and 20 PR (10 female) of the same age (10-12 years old). Two pure tones (1000 and 3000 Hz) were used in an oddball paradigm. No significant differences were observed in the amplitudes and latencies of N100 between the groups. However, N200 to frequent stimuli and P200 to both frequent and infrequent stimuli were of higher amplitude in poor readers than in normal children. There were no differences between groups in the latency and amplitude of P300. The results suggest that PR use more attentional resources in the components occurring before P300 to both frequent and infrequent stimuli than the normal children, and this finding is particularly marked for PR girls.     

Radionuclide studies in chronically hemodialyzed patients. Bone scintigraphy for the evaluation and control of renal osteopathy

The clinical applicability of bone scintigraphy (Tc99m MDP) was evaluated in 42 patients on maintenance hemodialysis. Typical scintigraphic findings are shown which were related to hormonal and biochemical parameters of calcium and phosphate metabolism. Visual grading of representative regions for metabolic bone disease in bone scans was compared to scintimetry which applies a bone to soft tissue ratio to grade osseous abnormalities. It could be shown that visual interpretation and grading of the findings according to a score is sufficient to assess the degree and extent of renal bone disease. Semiquantitative analysis of bone scintigrams by scintimetry did not improve the diagnostic information.     

Clinical and laboratory chemical examination in the diagnostic demarkation of chronic lymphatic leukemia

In its conventional meaning, chronic lymphatic leukemia (CLL) describes a variety of clinical and prognostical aspects. Since it was found using immunohistological methods that a remarkable number of lymphoplasmacytoid lymphoma (immunocytoma) were initially considered to belong to the CLL-group according to cell morphology, cell count and clinical status, the purpose of this study was to set up clinical and laboratory parameters separating CLL and immunocytoma. This was accomplished by categorizing 71 patients of preliminary CLL by age, sex, distribution of B and T lymphocytes, cell morphology, symptoms at the time of diagnosis, clinical course, electrophoresis and quantitative immunoglobulines. According to these features, the sample of patients was divided into three groups: Group 1: chronic lymphatic leukemia probable. Group 2: immunocytoma probable. Group 3: clear-cut separation is not possible. In these patients a lymphnode extirpation and a fluorescent examination of surface immunoglobulines should be performed.