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排序方式: 共有163条查询结果,搜索用时 15 毫秒
41.
Effect of the nonsteroidal anti-inflammatory drug indomethacin on proliferation and apoptosis of colon carcinoma cells 总被引:8,自引:0,他引:8
Kralj M Kapitanović S Kovacević D Lukac J Spaventi S Pavelić K 《Journal of cancer research and clinical oncology》2001,127(3):173-179
Purpose: Nonsteroidal anti-inflammatory drugs lower the incidence of and mortality from colon cancer. In this paper, we present the
effect of indomethacin on growth inhibition and alterations in the expression of several genes involved in cell cycle and
apoptosis in CaCo-2 colon adenocarcinoma cells. Methods: We used the MTT test to evaluate the effect of indomethacin on the proliferation rate of colon cancer and normal fibroblast
cells in vitro. The expression of c-myc oncoprotein and p53 and p27 suppressor proteins was examined using the immunocytochemical
method. Results: We have shown that indomethacin reduces the proliferation rate of CaCo-2 colon cancer cells (up to 60% at the concentration
of 4 × 10−4 M), alters their morphology, and induces cell death by apoptosis. The most pronounced inhibitory effect was observed at the
concentration of 6 × 10−4 M where the growth was completely suppressed. However, the growth of normal fibroblasts (Hef 522) was much less inhibited
(about 30% of inhibition at the concentration of 6 × 10−4 M). Indomethacin reduces the proliferation rate and induces apoptosis in CaCo-2 colon cancer cells through enhanced expression
of c-myc, p53, and p27 proteins. Conclusions: This is the first report about p27-increased expression in colon carcinoma cells induced by indomethacin treatment. Increased
expression of p27 represents a new mechanism of apoptosis in cells treated with NSAIDs (indomethacin). This effect probably
contributes to the anti-proliferative effect on colon cancer cells in vitro.
Received: 27 April 2000 / Accepted: 7 August 2000 相似文献
42.
Ceović R Lipozenčić J Bukvić Mokos Z Stulhofer Buzina D Kostović K 《Acta dermatovenerologica Croatica : ADC》2010,18(3):195-200
Numerous treatment modalities have been used to treat keloids and hypertrophic scars, but optimal treatment has not yet been established. The failure of achieving better therapeutic results in treating keloids highlights the essential problem that the pathogenetic mechanisms causing keloids remain unclear. Increased understanding at the molecular level will lead to the development of new therapies. Prevention is the first rule in keloid therapy. Conventional and experimental therapeutic approaches are presented in this review but further investigation is needed in relation to safety, adverse effects, and therapeutic efficacy. Because of the high recurrence rate of keloid scars, a follow-up period of at least 1 year is required to enable the start of treatment of recurrences as expediently as possible and to evaluate long-term success. 相似文献
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Recent progress in the field of molecular genetics revealed a broader spectrum of dystrophin-related disorders than previously assumed. In addition, the pathogenetic basis of other types of muscular dystrophies could be identified: some autosomal-recessive limb girdle dystrophies are caused by mutations of sarcoglycan genes, others are caused by deficiency of the sarcoplasmatic enzyme calpain-3. Emery-Dreifuss muscular dystrophy is due to the deficiency of the nuclear membrane protein emerin. About 50% of congenital muscular dystrophies are related to mutations of a extracellular matrix protein merosin (alpha-laminin). A series of monoclonal antibodies for immunohistochemistry is now available recognizing many cytoskeletal muscle proteins. In combination with molecular genetics a diagnostic flow chart can be developed which allows a definite diagnosis in most cases. In this review disease entities are illustrated by case reports. We discuss the significance of immunohistochemical and molecular methods for diagnosis. 相似文献
45.
More than 100 sera from patients with scleroderma CREST (calcinosis, Raynaud phenomenon, esophageal dismotility, sclerodactyly, telangiectasia) were tested in order to detect antigenic nuclear components of the field beanVicia faba (2n=12). Kinetochores of mitotic chromosomes and prekinetochores of interphase cells from root-tip meristems were specifically labelled via an indirect immunofluorescence procedure by antibodies of one of these sera. In 44% of interphase nuclei in which centromeres could be identified, only half (6) of the number of expected prekinetochores (12) was detected, circumstantially indicating at least transient association of homologous centromeres. Some nuclei showed clustering of centromeres at one pole (Rabl configuration). In metaphase chromosomes, each sister kinetochore contained a fluorescent spot. Western blotting of field bean nuclear proteins revealed four antigenic proteins of 28, 30, 64 and 68 kDa. 相似文献
46.
Nives Pećina-Ślaus Krešimir Pavelić Jasminka Pavelić 《Journal of molecular medicine (Berlin, Germany)》1999,77(5):446-453
This study evaluated the potential contribution of the APC gene to malignant transformation in patients with renal cell carcinoma.
We tested 36 human renal cell carcinoma samples and 18 adjacent normal kidney tissues for the expression of APC protein, both
wild and truncated types, by western blot using antibodies that recognize either the carboxy or the amino epitope of the APC
protein. The same tumor samples together with autologous peripheral blood were also analyzed at the DNA level. Using specific
oligonucleotide primers for exons 11 and 15, gene instability was followed by polymerase chain reaction/loss of heterozygosity
(LOH) (on the basis of restriction fragment length polymorphism). Molecular data were also compared to pathohistological diagnosis,
TNM stage, and patient’s age using multivariate statistical methods. All normal renal tissues revealed expression of the wild-type
APC protein. Neither wild nor mutant type proteins were found in 36% (13/36) of tumor samples; the rest of tumor tissues expressed
the wild-type protein (312 kDa). Mutated APC protein, with a molecular weight of 117 kDa, was found in only one tumor sample.
From 36 tumor samples 16 (44.4%) were informative for RsaI exon 11 polymorphic site, while only half of these (8/16) demonstrated LOH. From 13 tumor samples that had no detectable
protein product by western blot analysis eight were homozygous for the exon 11 polymorphism and were tested for another polymorphic
site, MspI/exon 15. The overall proportion of LOH cases for both polymorphisms tested was 52.9% (9/17). Pathohistological diagnosis
and molecular data showed no correlation. However, multivariate analysis determined a stage strong positive correlation of
age and TNM with the presence of LOH and the absence of the wild-type APC protein. Out results suggest that the APC tumor
suppressor gene plays a role in renal carcinogenesis. Alterations in this gene are responsible for tumor evolution and progression,
but cannot be considered as a first event in tumor initiation.
Received: 20 May 1998 / Accepted: 23 November 1998 相似文献
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Krešimir Pavelić Mirko Hadžija Ljiljana Bedrica Jasminka Pavelić Ivan Ðikić Maša Katić Marijeta Kralj Maja Herak Bosnar Sanja Kapitanović Marija Poljak-Blaži Šimun Križanac Ranko Stojković Mislav Jurin Boris Subotić Miroslav Čolić 《Journal of molecular medicine (Berlin, Germany)》2001,78(12):708-720
Natural silicate materials, including zeolite clinoptilolite, have been shown to exhibit diverse biological activities and have been used successfully as a vaccine adjuvant and for the treatment of diarrhea. We report a novel use of finely ground clinoptilolite as a potential adjuvant in anticancer therapy. Clinoptilolite treatment of mice and dogs suffering from a variety of tumor types led to improvement in the overall health status, prolongation of life-span, and decrease in tumors size. Local application of clinoptilolite to skin cancers of dogs effectively reduced tumor formation and growth. In addition, toxicology studies on mice and rats demonstrated that the treatment does not have negative effects. In vitro tissue culture studies showed that finely ground clinoptilolite inhibits protein kinase B (c-Akt), induces expression of p21WAF1/CIP1 and p27KIP1 tumor suppressor proteins, and blocks cell growth in several cancer cell lines. These data indicate that clinoptilolite treatment might affect cancer growth by attenuating survival signals and inducing tumor suppressor genes in treated cells. 相似文献
50.