卵细胞质内单精子注射治疗不孕症34例临床总结

目的总结应用单精子卵细胞质内注射（ICSI）治疗不孕症的临床效果。方法对34对男性因素、原因不明、受精障碍不孕患者采用GnRHa长周期促排卵方案,给有第一极体的卵细胞行单精子注射。结果34例34个治疗周期中,1例因反应不良取消。取卵数378个,注射卵数317（83．9％）个,正常受精卵231个（72．9％）卵裂228（98．3％）个,良好胚胎99（43．4％）个,平均移植胚胎数36个,临床妊娠12例（移植周期妊娠率为36.4％）。结论ICSI是治疗男性因素、原因不明、受精障碍等因素引起不孕症的一种切实可行的方法。     

慢性低浓度二硫化碳接触作业工人血脂的变化

对某化纤厂99名长期接触低浓度二硫化碳（CS2）作业工人和28名同厂非CS2作业工人的血脂进行流行病学横断面调查。结果发现：除接触组脂蛋白（a）［Lp（a）］浓度明显高于对照组外,其它血脂指标差别均无统计学意义;进一步通过多元逐步回归分析发现,长期低浓度CS2接触对Lp（a）的影响最大,二者呈正相关（r＝0．43,P＜0．01）。提示Lp（a）可能在CS2致脂质代谢障碍毒作用机制中起着重要作用。     

后入路手术治疗胸腰椎骨折的若干问题

目的 减少后入路治疗胸腰椎骨折的手术失误,提高手术成功率,方法及结果 对３５６例胸腰椎骨折伴脊髓损伤患者进行回顾性分析,发现治疗失误４５例,其中内固定器材选择不当２例,椎体定位错误５例,椎弓根螺钉进钉点错误２例,进钉方向不准确５例,椎管内有残留骨块致减压不彻底１４例,植骨不理想致拔钉后再移位２例,手术方式选择不当４例,医源性造成关节突骨折３例,硬膜破裂２例,骨折脱位复痊不满意２例,负重或参加体力劳     

130例国人正常胎儿手足长的测量

本文130例国人胎儿的手、足长测量结果表明:手、足长与胎龄线性相关(手∶r=0.9498;足∶r=0.963)。从而可据手或足长预测胎龄。结果还表明:左、右手长度无显著性差异(P<0.01)。     

A K ow -Based QSAR Model for Predicting Toxicity of Halogenated Benzenes to all Algae Regardless of Species

In this study, a total of 40 tests of the toxicity of 8 halogenated benzenes to five algal species were performed. The result demonstrated that the toxicity of halogenated benzenes to five algal species was directly related to the hydrophobicity of the chemicals and the lipid content of the algae. Based on the results, we developed a K(ow)-based quantitative structure-activity relationship (QSAR) model: log(1/EC??) = 1.050 logK(ow) + 1.429 log(1/lipid)-3.224 with n = 40, r2 = 0.946, S.E. = 0.211, F = 323.933 at p < 0.001. This model provides evidence that the toxicity of halogenated benzenes to these five algae tested is related to the slower transference into lipid. This model can potentially be generalized to other algal species and toxicants.     

武汉市外环境中禽流行性感冒病毒分布及禽类职业暴露人群H5N1抗体水平调查

目的 监测武汉市外环境中禽流行性感冒(简称禽流感)病毒的分布情况,了解禽类职业暴露人群高致病性禽流感病毒(H5N1)的感染状况.方法 2010年从3个野生候鸟柄息地和5个城乡活禽市场采集78份外环境标本(水、禽类粪便及笼具表面标本),从13个禽流感监测点采集禽类职业暴露人群的血清标本249份.采用实时荧光定量RT-PCR方法,对禽流感外环境标本进行甲型流感病毒检测,对于甲型流感病毒阳性的标本,进一步采用多重RT-PCR方法,进行H3、H5、H7、H9亚型禽流感病毒分型.采用马红细胞凝集抑制试验和2种禽流感灭活抗原(A/Hubei/1/10和A/Anhui/1/05)对职业暴露人群血清中高致病性禽流感H5N1抗体水平进行检测.结果 采自城乡活禽市场的50份外环境标本中,17份(34.0％)为甲型流感病毒阳性,其中H5亚型单纯阳性标本4份,H9亚型单纯阳性标本3份,H3、H5亚型混合阳性标本4份,H3、H9亚型混合阳性标本2份,H5、H9亚型混合阳性标本2份,H3、H5、H9亚型混合阳性标本2份,未检测到H7阳性标本.采自野生候鸟栖息地的28份外环境标本,未检测到甲型流感病毒阳性标本.不同类型外环境标本甲型流感病毒阳性率分别为:水标本37.5％ (6/16),笼具表面标本16.7％( 5/30),粪便标本l8.8％ (6/32).对A/Hubei/1/10抗原抑制效价≥40的血清标本共100份,占标本总数的40.2％,而对A/Anhui/1/05抗原抑制效价≥40的血清标本共36份,占标本总数的14.5％,两组间差异有统计学意义(x2=41.433,P＜0.05).249份职业暴露人群血清标本中,针对A/Hubei/1/10抗原的H5N1抗体阳性标本5份(抑制效价≥160),均来自城乡活禽市场,而针对A/Anhui/1/05抗原则无H5N1抗体阳性标本.结论 武汉市城乡活禽市场内流行着多种亚型禽流感病毒,外环境禽流感病毒分布与禽类职业暴露人群H5N1抗体水平检测结果一致.     

Associations between snacking and weight loss and nutrient intake among postmenopausal overweight to obese women in a dietary weight-loss intervention

Snacking may play a role in weight control. The associations of timing and frequency of snacking with observed weight change and nutrient intake were assessed in an ancillary study to a 12-month randomized controlled trial in Seattle, WA. Overweight-to-obese postmenopausal women (n=123) enrolled in the two dietary weight-loss arms from 2007 to 2008 with complete data at 12 months were included in these analyses. Generalized linear models were used to test the associations between snacking and weight loss (percent) and nutrient intake at the 12-month time point. Participants were, on average, 58 years old and mainly non-Hispanic white (84%). Ninety-seven percent reported one or more snacks per day. Weight loss (percent) was significantly lower among mid-morning (10:30 am to 11:29 am) snackers (7.0%, 95% confidence interval: 4.3 to 9.7) compared to non–mid-morning snackers (11.4%, 95% confidence interval: 10.2 to 12.6; P=0.005). A higher proportion of mid-morning snackers reported more than one snack per day (95.7%), compared to afternoon (82.8%) and evening (80.6%) snackers, although differences were not statistically significant (P>0.05). Women who reported two or more snacks per day vs one or no snacks per day had higher fiber intake (P=0.027). Afternoon snackers had higher fruit and vegetable intake compared to non–afternoon-snackers (P=0.035). These results suggest that snack meals can be a source for additional fruits, vegetables, and fiber-rich foods; however, snacking patterns might also reflect unhealthy eating habits and impede weight-loss progress. Future dietary weight-loss interventions should evaluate the effects of timing, frequency, and quality of snacks on weight loss.     

地震后汶川县映秀镇的小型兽类组成及季节动态观察

目的了解四川省汶川县地震后小型兽类的组成和季节动态,为预防地震后鼠害与鼠传疾病暴发提供参考依据。方法于2009年7月（夏）、10月（秋）,2010年1月（冬）、4月（春）采用夹夜法对四川省汶川县映秀镇进行小型兽类调查取样、统计分析。结果调查期间共布放1645夹次,捕获率为4.38%,捕获小兽隶属于2目2科6属9种,分别为四川短尾鼩、黄胸鼠、大足鼠、北社鼠、小家鼠、高山姬鼠、褐家鼠、中华姬鼠和灰麝鼩,优势种为北社鼠和四川短尾鼩;多样性指数、均匀性指数和优势度指数分别为2.574、0.205和0.812;从夏季到春季,小兽密度先上升再降低,物种数、多样性指数、均匀性指数总体降低,优势度指数总体升高,且各指数呈双峰型变化。结论映秀镇在地震后不同季节小型兽类组成不同,应根据各季节优势种和密度变化采取相应防治措施。     

临床路径在单病种限价中应用的研究

目的 为遏制医疗费用的过快增长,缓解“看病难、看病贵”现象,提高医疗质量.方法 通过对200例无基础疾病,年龄、诊断、病情、手术方式、麻醉方式、护理常规和术后并发症无差异的胆囊炎、胆结石患者实施腹腔镜手术的病例,其中100例依据临床路径实施单病种限价;另100例依常规检查、手术、治疗、护理等按项目收费的研究比较;结果 ...     

硬皮病皮损成纤维细胞整合素表达的免疫电镜观察*

目的：研究硬皮病皮损成纤维细胞整合素表达及细胞超微结构特征。方法：将培养的硬皮病皮损成纤维细胞置包被纤维粘连蛋白的培养瓶中作粘附培养,运用间接免疫胶体金技术在电镜下观察成纤维细胞表面整合素α5,β1亚基表达、分布特征及细胞超微结构变化。结果：硬皮病皮损成纤维细胞整合素α5,β1亚基表达均较正常成纤维细胞显著增加（P<0.01）,并呈簇集分布的特征;细胞内骨架成分微丝密集成束。结论：整合素α5,β1介导的纤维粘连蛋白与成纤维细胞相互作用在硬皮病时增强。