全文获取类型
收费全文 | 13663篇 |
免费 | 659篇 |
国内免费 | 96篇 |
专业分类
耳鼻咽喉 | 188篇 |
儿科学 | 265篇 |
妇产科学 | 146篇 |
基础医学 | 1747篇 |
口腔科学 | 268篇 |
临床医学 | 784篇 |
内科学 | 3689篇 |
皮肤病学 | 176篇 |
神经病学 | 1107篇 |
特种医学 | 562篇 |
外科学 | 2588篇 |
综合类 | 67篇 |
一般理论 | 2篇 |
预防医学 | 329篇 |
眼科学 | 119篇 |
药学 | 740篇 |
中国医学 | 20篇 |
肿瘤学 | 1621篇 |
出版年
2024年 | 14篇 |
2023年 | 92篇 |
2022年 | 202篇 |
2021年 | 341篇 |
2020年 | 166篇 |
2019年 | 267篇 |
2018年 | 322篇 |
2017年 | 265篇 |
2016年 | 321篇 |
2015年 | 352篇 |
2014年 | 415篇 |
2013年 | 521篇 |
2012年 | 881篇 |
2011年 | 891篇 |
2010年 | 562篇 |
2009年 | 463篇 |
2008年 | 880篇 |
2007年 | 953篇 |
2006年 | 896篇 |
2005年 | 897篇 |
2004年 | 896篇 |
2003年 | 833篇 |
2002年 | 844篇 |
2001年 | 172篇 |
2000年 | 145篇 |
1999年 | 171篇 |
1998年 | 225篇 |
1997年 | 153篇 |
1996年 | 144篇 |
1995年 | 98篇 |
1994年 | 99篇 |
1993年 | 100篇 |
1992年 | 92篇 |
1991年 | 71篇 |
1990年 | 71篇 |
1989年 | 63篇 |
1988年 | 67篇 |
1987年 | 57篇 |
1986年 | 40篇 |
1985年 | 49篇 |
1984年 | 41篇 |
1983年 | 38篇 |
1982年 | 23篇 |
1981年 | 25篇 |
1980年 | 30篇 |
1979年 | 21篇 |
1978年 | 21篇 |
1977年 | 17篇 |
1976年 | 15篇 |
1975年 | 15篇 |
排序方式: 共有10000条查询结果,搜索用时 0 毫秒
101.
Shibuya H Hamamura K Hotta H Matsumoto Y Nishida Y Hattori H Furukawa K Ueda M Furukawa K 《Cancer science》2012,103(9):1656-1664
The expression and implications of gangliosides in human osteosarcomas have not been systematically analyzed. In this study, we showed that gangliosides GD3 and GD2 are highly expressed in the majority of human osteosarcoma cell lines derived from oral cavity regions. Introduction of GD3 synthase cDNA into a GD3/GD2-negative (GD3/GD2-) human osteosarcoma subline resulted in the establishment of GD3/GD2+ transfectant cells. They showed increased cell migration and invasion activities in wound healing and Boyden chamber invasion assays, respectively, compared to the control cells. When treated with serum, GD3/GD2+ cells showed stronger tyrosine phosphorylation of p130Cas, focal adhesion kinase, and paxillin than GD3/GD2- cells. In particular, paxillin underwent much stronger phosphorylation, suggesting its role in cell motility. Furthermore, we tried to dissect the roles of GD3 and GD2 in the malignant properties of the transfectant cells by establishing single ganglioside-expressing cells, that is, either GD3 or GD2. Although GD3/GD2+ cells showed the most malignant properties, GD2+ cells showed almost equivalent levels to GD3/GD2+ cells in invasion and migration activities, and in the intensities of tyrosine phosphorylation of paxillin. Among Src family kinases, Lyn was expressed predominantly, and was involved in the invasion and motility of GD3- and/or GD2-expressing transfectants. Furthermore, it was elucidated by gene silencing that Lyn was located in a different pathway from that of FAK to eventually lead paxillin activation. These results suggested that GD2/GD3 are responsible for the enhancement of the malignant features of osteosarcomas, and might be candidate targets in molecular-targeted therapy. 相似文献
102.
Kanto S Sugawara J Masuda H Sasano H Arai Y Kyono K 《Fertility and sterility》2008,90(5):2010.e5-2010.e7
103.
104.
Yukio Suzuki Akiko Sukegawa Yutaka Ueda Masayuki Sekine Takayuki Enomoto Alexander Melamed Jason D Wright Etsuko Miyagi 《JMIR Public Health and Surveillance》2022,8(5)
BackgroundProviding adequate information to parents who have children eligible for human papillomavirus (HPV) vaccination is essential to overcoming vaccine hesitancy in Japan, where the government recommendation has been suspended. However, prior trials assessing the effect of brief educational tools have shown only limited effects on increasing the willingness of parents to vaccinate their daughters.ObjectiveThe aim of this trial is to assess the effect of a cervical cancer survivor’s story on the willingness of parents to get HPV vaccination for their daughters.MethodsIn this double-blinded, randomized controlled trial (RCT) implemented online, we enrolled 2175 participants aged 30-59 years in March 2020 via a webpage and provided them with a questionnaire related to the following aspects: awareness regarding HPV infection and HPV vaccination, and willingness for HPV vaccination. Participants were randomly assigned (1:1) to see a short film on a cervical cancer survivor or nothing, stratified by sex (male vs female) and willingness for HPV vaccination prior to randomization (yes vs no). The primary endpoint was the rate of parents who agreed for HPV vaccination for their daughters. The secondary endpoint was the rate of parents who agreed for HPV vaccination for their daughters and the HPV vaccination rate at 3 months. The risk ratio (RR) was used to assess the interventional effect.ResultsOf 2175 participants, 1266 (58.2%) were men and 909 (41.8%) were women. A total of 191 (8.8%) participants were willing to consider HPV vaccination prior to randomization. Only 339 (15.6%) participants were aware of the benefits of HPV vaccination. In contrast, 562 (25.8%) participants were aware of the adverse events of HPV vaccination. Although only 476 (21.9%) of the respondents displayed a willingness to vaccinate their daughters for HPV, there were 7.5% more respondents in the intervention group with this willingness immediately after watching the short film (RR 1.41, 95% CI 1.20-1.66). In a subanalysis, the willingness in males to vaccinate daughters was significantly higher in the intervention group (RR 1.50, 95% CI 1.25-1.81); however, such a difference was not observed among females (RR 1.21, 95% CI 0.88-1.66). In the follow-up survey at 3 months, 1807 (83.1%) participants responded. Of these, 149 (8.2%) responded that they had had their daughters receive vaccination during the 3 months, even though we could not see the effect of the intervention: 77 (7.9%) in the intervention group and 72 (8.7%) in the control group.ConclusionsA cervical cancer survivor’s story increases immediate willingness to consider HPV vaccination, but the effect does not last for 3 months. Furthermore, this narrative approach to parents does not increase vaccination rates in children eligible for HPV vaccination.Trial RegistrationUMIN Clinical Trials Registry UMIN000039273; https://tinyurl.com/bdzjp4yf 相似文献
105.
Tsung-Che Chang Yoshiyuki Manabe Keita Ito Ryuku Yamamoto Kazuya Kabayama Shino Ohshima Yoshie Kametani Yukari Fujimoto Chun-Cheng Lin Koichi Fukase 《RSC advances》2022,12(29):18985
Sialyl-Tn (STn), overexpressed on various tumors, has been investigated for its application in anti-cancer vaccine therapy. However, Theratope, an STn-based vaccine, failed in the phase III clinical trial due to poor immunogenicity and epitope suppression by the foreign carrier protein. We therefore developed a self-adjuvanting STn based-vaccine, a conjugate of clustered STn (triSTn) antigen, TLR1/2 ligand (Pam3CSK4), and T-helper (Th) cell epitope, and found that this three-component self-adjuvanting vaccine effectively resulted in the production of anti-triSTn IgG antibodies. We herein analyzed immune responses induced by this self-adjuvanting vaccine in detail. We newly synthesized two-component vaccines, i.e., Pam3CSK4- or Th epitope-conjugated triSTn, as references to evaluate the immune-stimulating functions of Pam3CSK4 and Th epitope. Immunological evaluation of the synthesized vaccine candidates revealed that Pam3CSK4 was essential for antibody production, indicating that the uptake of triSTn antigen by antigen-presenting cells (APCs) was promoted by the recognition of Pam3CSK4 by TLR1/2. The function of the Th epitope was also confirmed. Th cell activation was important for boosting antibody production and IgG subclass switching. Furthermore, flow cytometric analyses of immune cells, including T cells, B cells, dendritic cells, and other monocytes, were first employed in the evaluation of self-adjuvanting vaccines and revealed that the three-component vaccine was able to induce antigen-specific immune responses for efficient antibody production without excessive inflammatory responses. Importantly, the co-administration of Freund''s adjuvants was suggested to cause excessive myeloid cell accumulation and decreased plasma cell differentiation. These results demonstrate that vaccines can be designed to achieve the desired immune responses via the bottom-up construction of each immune element.Detailed analysis of a three-component self-adjuvanting vaccine revealed that conjugate vaccines can be designed to achieve the desired immune responses via bottom-up construction of the necessary immune elements. 相似文献
106.
Masaki Uchihara Jun Ehara Keiichi Iwanami Koichi Kitamura Toshihiko Suzuki Noriyoshi Ishizuka Toru Yamada Eiji Hiraoka 《Internal medicine (Tokyo, Japan)》2022,61(13):1995
Chylous ascites (CA) is the accumulation of fluid with a high triglyceride content in the peritoneal cavity. Only two cases in the literature have reported CA with hyperthyroidism. A 28-year-old previously healthy woman presented with gradual-onset abdominal swelling, exertional dyspnea, and diarrhea. Hyperthyroidism and heart failure were diagnosed using laboratory investigation and echocardiography. Ultrasonography revealed a large amount of ascites. The ascitic fluid was milky with elevated triglyceride levels. Treatment with anti-thyroid therapy and diuretics improved all symptoms, and the free triiodothyronine (T3) level normalized after five days. Hyperthyroidism and heart failure should be considered as reversible causes of CA. 相似文献
107.
Yuta Yamamura Kengo Furuichi Tadashi Toyama Megumi Oshima Hisayuki Ogura Koichi Sato Shiori Nakagawa Taro Miyagawa Shinji Kitajima Akinori Hara Yasunori Iwata Norihiko Sakai Miho Shimizu Hiroko Ikeda Tomoko Toma Kazuya Takasawa Akihiro Yachie Takashi Wada 《Internal medicine (Tokyo, Japan)》2022,61(7):1105
We herein report a 36-year-old man with repeated necrotizing lymphadenitis due to MEFV gene mutations. The patient''s chief complaints were a fever and painful cervical lymphadenopathy. We diagnosed him with necrotizing lymphadenitis based on the pathological findings of the lymph nodes and the exclusion of other differential diseases. The same episode recurred four times. We speculated the involvement of autoinflammatory backgrounds and detected MEFV gene mutations of E148Q (homo), P369S, and R408Q. Considering the elevation of interleukin-18, these mutations probably played roles in the repeated necrotizing lymphadenitis. 相似文献
108.
Kenji Nakahama Hiroyasu Kaneda Koichi Ogawa Yoshiya Matsumoto Yoko Tani Tomohiro Suzumura Shigeki Mitsuoka Tetsuya Watanabe Kazuhisa Asai Tomoya Kawaguchi 《Internal medicine (Tokyo, Japan)》2022,61(7):1039
A 68-year-old Japanese man was diagnosed with lung adenocarcinoma stage IVB. We introduced a first-line chemotherapy of four cycles of carboplatin and pemetrexed and pembrolizumab, followed by pemetrexed and pembrolizumab maintenance therapy. Approximately four months after anticancer therapy, a small nodule appeared in the right peripheral S3 lesion. After five months, the nodule was confirmed as a Mycobacterium tuberculosis (TB) nodule. We initiated anti-TB therapy without stopping pembrolizumab, and the right S3 nodule shrank immediately. This report supports the concurrent use of anti-TB treatment with an immune checkpoint inhibitor when the TB infection area is limited. 相似文献
109.
Pancreas-Sparing Duodenectomy for a Recurrent Retroperitoneal Liposarcoma: Report of a Case 总被引:1,自引:0,他引:1
Kimura Y Mukaiya M Honma T Okuya K Akizuki E Kihara C Furuhata T Hata F Katsuramaki T Tsukamoto T Hirata K 《Surgery today》2005,35(1):91-93
Pancreas-sparing duodenectomy (PSD), which allows preservation of the pancreas in its entirety, is a promising procedure for low-grade malignancies of the duodenum, the periampullary region, and the neighboring retroperitoneum. We report a case of recurrent retroperitoneal liposarcoma involving the second and third parts of the duodenum, which was extirpated using PSD, after a right hemicolectomy for tumor invasion of part of the colonic hepatic flexura. The Roux-en-Y jejunal limb was sutured to the duodenal bulb in an end-to-end fashion, and the biliary and pancreatic duct systems were reconstructed with end-to-side anastomoses, placing the jejunal limb distal to the bulbo-jejunostomy after a septoplasty to repair the adjacent pancreatic and biliary ducts. Because retroperitoneal liposarcoma has a low incidence of lymph node metastasis, pancreaticoduodenectomy may be inappropriate, especially if minimally extensive surgery can ensure organ preservation. Pancreas-sparing duodenectomy could be the most appropriate procedure for nonepithelial malignant tumors located around the duodenum distal to the pylorus, which have no involvement with the pancreatic parenchyma or periduodenal lymph nodes. 相似文献
110.
Pohoey Fan M.D. Koichi Kimura M.D. Ph.D. Katsu Takenaka M.D. Ph.D. F.J.C.C. Aya Ebihara M.D. Ph.D. Tomoko Okano B.S. Kansei Uno M.D. Ph.D. Nobuaki Fukuda M.D. Jiro Ando M.D. Ph.D. Hideo Fujita M.D. Ph.D. Hiroyuki Morita M.D. Ph.D. Yutaka Yatomi M.D. Ph.D. Ryozo Nagai M.D. Ph.D. F.J.C.C. 《Echocardiography (Mount Kisco, N.Y.)》2012,29(4):403-403
Article Title: Speckle Tracking Global Strain Rate E/E’ Predicts LV Filling Pressure More Accurately Than Traditional Tissue Doppler E/E’ (Echocardiography, 2012;29:403) 相似文献