全文获取类型
收费全文 | 352篇 |
免费 | 17篇 |
国内免费 | 47篇 |
专业分类
耳鼻咽喉 | 1篇 |
儿科学 | 10篇 |
妇产科学 | 11篇 |
基础医学 | 29篇 |
口腔科学 | 13篇 |
临床医学 | 77篇 |
内科学 | 52篇 |
皮肤病学 | 4篇 |
神经病学 | 44篇 |
特种医学 | 89篇 |
外科学 | 24篇 |
综合类 | 17篇 |
预防医学 | 10篇 |
眼科学 | 5篇 |
药学 | 23篇 |
肿瘤学 | 7篇 |
出版年
2021年 | 1篇 |
2020年 | 1篇 |
2019年 | 2篇 |
2018年 | 2篇 |
2017年 | 2篇 |
2016年 | 5篇 |
2015年 | 7篇 |
2014年 | 4篇 |
2013年 | 9篇 |
2012年 | 11篇 |
2011年 | 2篇 |
2010年 | 12篇 |
2009年 | 13篇 |
2008年 | 6篇 |
2007年 | 31篇 |
2006年 | 15篇 |
2005年 | 18篇 |
2004年 | 11篇 |
2003年 | 8篇 |
2002年 | 10篇 |
2001年 | 12篇 |
2000年 | 8篇 |
1999年 | 15篇 |
1998年 | 20篇 |
1997年 | 23篇 |
1996年 | 9篇 |
1995年 | 14篇 |
1994年 | 8篇 |
1993年 | 10篇 |
1992年 | 6篇 |
1991年 | 3篇 |
1990年 | 9篇 |
1989年 | 8篇 |
1988年 | 8篇 |
1987年 | 13篇 |
1986年 | 10篇 |
1985年 | 13篇 |
1984年 | 5篇 |
1983年 | 10篇 |
1982年 | 6篇 |
1981年 | 4篇 |
1980年 | 6篇 |
1979年 | 6篇 |
1978年 | 2篇 |
1977年 | 1篇 |
1976年 | 7篇 |
1975年 | 4篇 |
1974年 | 3篇 |
1973年 | 1篇 |
1972年 | 1篇 |
排序方式: 共有416条查询结果,搜索用时 15 毫秒
81.
82.
下消化道出血221例分析 总被引:2,自引:2,他引:2
0 引言 下消化道出血是消化科的常见病 ,是指十二指肠空肠移行部 ,屈氏韧带以下的小肠和大肠疾病引起的肠道出血 .有人认为一般不包括痔和肛裂出血 [1 ] .临床最常见为慢性出血 ,但有时出血量大危及生命 ,需要做紧急处理 ,现将我院1992 - 0 1/ 1999- 0 8门诊及病房收治的 2 2 1例下消化道出血进行分析 ,报告如下 :1 临床资料 男 12 0例 ,女 10 1例 ,年龄 14~ 72 (平均 42 )岁 ,病程 16 h~ 10 a.患者分别以脓血便、暗红色血便、鲜血便或果酱色血便为主诉前来就诊 ,其中有休克症状的大出血者6例 .血 Hb<110 g·L- 1 40例 ,<80 g·L- 1… 相似文献
83.
从头序木(AraliadasyphylaMiq)叶中分离到6个三萜化合物,用化学方法和波谱(UV,IR,MS,1HNMR,13CNMR,13CDEPT,HMQC,HMBC)分析,确定其中4种化合物的结构,分别为齐墩果酸(I),16β羟基18βH齐墩果酸(I),齐墩果酸28OβD吡喃葡萄糖甙(II)和16β羟基18βH齐墩果酸28OβD吡喃葡萄糖甙(IV)。用NOESY谱确定化合物I的立体构型。以上4个化合物均为首次从该植物中分离到,化合物IV为新化合物。 相似文献
84.
Jiang WZ Jin NY Li ZJ Zhang LS Wang HW Zhang YJ Han WY 《第二军医大学学报》2006,27(4):434-434
To express the core protein of HIV-1 of Chinese prevalent strain (HIV-1 (CN)) in Pichia pastoris, the fulllength gag gene was inserted into the secretory expression vector pHILS1. Linearized recombinant plasmid pHILGAG by Sail was electrotransformed into the yeast strain GS115, and the yeast transformants were identified by PCR. To induce the interest protein to be expressed, the PCR positive transformants were inoculated in the medium of BMGY and BMMY, mRNA of the strain was detected by RT-PCR, and the expressed protein was analyzed by SDS-PAGE, Western blotting and thin layer scanning. mRNA (1.3 kb) was amplified by RT-PCR. SDS-PAGE and Western blotting analysis showed that the molecular mass of the expressed protein was 55 kD, which was similar to the expected value, and the expressed protein could react with McAb to HIV-1 p24. Thin layer scanning analysis demonstrated that the whole amount of the expressed protein was approximately 13 % of the soluble protein in the supernatant. The recombinant yeast had good genetic stability. The optimal expression conditions of the engineering yeast were as follows: BMMY medium, 80-90% of dissolved oxygen, 1% methanol, and 3-day-cultivation course. Gag proteins were expressed under the optimal expression condition and purified via gel filtration chromatography. The purity of the interest protein was up to 85 %. After the purified proteins were inoculated into BALB/c mice, the anti-HIV-1 antibodies in the immunized mice could be detected by Western blotting. 相似文献
85.
目的 骨唾液酸蛋白(Bone sialoprotein,简称BSP)是由成骨细胞分泌的一种非胶原蛋白质,反映骨转换和骨形成的指标。最近研究认为:血清BSP浓度可反映破骨细胞活性和骨吸收过程,也可能是一个骨吸收指标。本实验测定了血清BSP在人体内的生物变异性。方法 采用RIA 法测定了290例不同性别和年龄的正常人血清BSP的正常值,血清BSP的天-天变异性及24 h 生物周期。 结果 在儿童组血清BSP正常水平明显高于成人组,其最高值在新生儿期和青春期。绝经后妇女其血清浓度比绝经前妇女水平明显升高(P< 0.05)。血清BSP在每天同一时间的波动范围在 7.3% 至17.7% (平均11.7% )。24 h 内有一个明显的生物周期性变化,表现为峰值在凌晨4~8 时,然后逐渐下降直到午后14时为最低。其生物周期的最大波幅为±20% ,(平均血浓度为10.5 ng/m l)。结论 血清BSP反映了骨转换的生理变化与年龄有明显相关性,其血清水平24 h 内有一个明显的生物周期,而天与天之间变异性较小。 相似文献
86.
Justus HW Jansen Olav P van der Jagt Bas J Punt Jan AN Verhaar Johannes PTM van Leeuwen Harrie Weinans Holger Jahr 《BMC musculoskeletal disorders》2010,11(1):188
Background
Although pulsed electromagnetic field (PEMF) stimulation may be clinically beneficial during fracture healing and for a wide range of bone disorders, there is still debate on its working mechanism. Mesenchymal stem cells are likely mediators facilitating the observed clinical effects of PEMF. Here, we performed in vitro experiments to investigate the effect of PEMF stimulation on human bone marrow-derived stromal cell (BMSC) metabolism and, specifically, whether PEMF can stimulate their osteogenic differentiation. 相似文献87.
陆金春等[1]所著的"中国118家实验室精液分析状况的调查"一文主要回顾了当前在中国采用的精液分析方法. 相似文献
88.
Shao-Chieh Lin Po-Chuan Chen Chung-Ta Lee Hong-Ming Tsai Peng-Chan Lin Helen HW Chen Yuan-Hwa Wu Bo-Wen Lin Wen-Pin Su Jenq-Chang Lee 《World journal of gastroenterology : WJG》2013,19(11):1797-1804
AIM:To investigate the 10-year results of treating low rectal cancer by a single surgeon in one institution.METHODS:From Oct 1998 to Feb 2009,we prospectively followed a total of 62 patients with cT2-4 low rectal cancer with lower tumor margins measuring at 3 to 6 cm above the anal verge.All patients received neoadjuvant chemoradiation(CRT) for 6 wk.Among them,85% of the patients received 225 mg/m2/d 5-fluorouracil using a portable infusion pump.The whole pelvis received a total dose of 45 Gy of irradiation in 25 fractions over 5 wk.The interval from CRT completion to surgical intervention was planned to be approximately 6-8 wk.Total mesorectal excision(TME) and routine defunctioning stoma construction were performed by one surgeon.The distal resection margin,circumferential resection margin,tumor regression grade(TRG) and other parameters were recorded.We used TRG to evaluate the tumor response after neoadjuvant CRT.We evaluated anal function outcomes using the Memorial Sloan-Kettering Cancer Center anal function scores after closure of the defunctioning stoma.RESULTS:The median distance from the lower margin of rectal cancer to the anal verge was 5 cm:6 cm in 9 patients,5 cm in 32 patients,4 cm in 10 patients,and 3 cm in 11 patients.Before receiving neoadjuvant CRT,45 patients(72.6%) had a cT3-4 tumor,and 21(33.9%) patients had a cN1-2 lymph node status.After CRT,30 patients(48.4%) had a greater than 50% clinical reduction in tumor size.The final pathology reports revealed that 33 patients(53.2%) had a ypT3-4 tumor and 12(19.4%) patients had ypN1-2 lymph node involvement.All patients completed the entire course of neoadjuvant CRT.Most patients developed only Grade 1-2 toxicities during CRT.Thirteen patients(21%) achieved a pathologic complete response.Few post-operative complications occurred.Nearly 90% of the defunctioning stomas were closed within 6 mo.The local recurrence rate was 3.2%.Pathologic lymph node involvement was the only prognostic factor predicting disease recurrence(36.5% vs 76.5%,P = 0.006).Ne 相似文献
89.
M Houpt S Koenigsberg R G Rosivack E W Baker 《Journal of the New Jersey Dental Association》1989,60(2):41-46
This study was performed in order to record the prevalence of tooth decay in high school students in New Jersey. During the Spring of 1986, 3,556 students, aged 13 to 18 years, in 8 public high schools in 7 communities, participated in the study. The subjects were examined by 3 trained examiners with mirror, explorer, air syringe and overhead auxiliary light. A lesion was recorded as dental caries if there was a perceptible break in the enamel surface. Questionable incipient areas were not recorded as carious lesions. Mean DMFT was found to be 4.17 +/- 3.7 and mean DMFS was 7.02 +/- 7.4. Hispanic subjects had more decay (DMFT 4.98, DMFS 9.03) than black subjects (DMFT 4.13, DMFS 6.94) and white subjects (DMFT 3.53, DMFS 5.43). Females had more decay (DMFT 4.51, DMFS 7.67) than males (DMFT 3.79, DMFS 6.33). Hispanic and black subjects also had more untreated decay (24 and 22 percent decayed of the DMFT) compared with only 11% untreated decay in white subjects. Socioeconomic level was inversely related to caries activity; however, geographic location of the school within the state (north versus south) was not a factor. 相似文献
90.